A brief update to the focal S:N501 builds to start your Fri…

There are 22 new non-UK sequences in 501Y.V1, including for the first time from Luxembourg (3), & additional seqs from Singapore (1), Australia (4), France (12), & Switzerland (2).

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First, Luxembourg has 3 sequences in 501Y.V1 for the first time. Each represents a separate introduction.

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France has 12 new sequences (orange). Many of these represent independent introductions.

Zooming in (divergence view), we can see 2 new sequences are identical (possible local transmission), & 2 differ by 2 mutations (1 each).

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Australia has 4 new sequences (red and orange) which are spread across the tree, representing separate additional introductions.

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Switzerland has 2 new sequences. One (marked 1st pic) is a clear separate introduction. The other (marked 2nd pic, zoomed, divergence) is from Bern & identical to an earlier sequence from Zurich ('ZH'). This could be local transmission or a common exposure.

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There is 1 new sequence from Singapore (orange).

Zooming in (divergence view), we can see it is identical to 2 older sequences, which may indicate local transmission or a common exposure.

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There are ~19 new non-South African sequences in 501Y.V2, including for the first time from Botswana (6), South Korea (1), & France (1), as well as additional sequences from the UK (~9).

(UK data may be affected by subsampling due to large sequence numbers.)

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At least 9 new sequences from the UK are now in 501Y.V2 (UK data may be impacted by subsampling).

These are a mix of separate introductions & local transmission.

Connections to French sequences are in the next tweet...

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France has 2 sequences in 501Y.V2. They cluster together, which may indicate local transmission or a common source.
They are related to seqs from England, but whether this is European transmission or exposure to common diversity in S Africa can't be distinguished clearly.

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Botswana has 6 sequences in 501Y.V2. One sits apart from the others, indicating at least 2 introductions.

The other 5 cluster together, but are separated by a 1-3 mutations. This could indicate local transmission or transmission from unsampled South African diversity.

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Lastly, South Korea has a sequence in 501Y.V2 for the first time.

12/14

As previously, the country plots are also updated. Lithuania is now added!

Remember: many countries are currently focusing sequencing efforts on S:N501 variants, so frequencies are often not true representations of the 501Y variants.

13/14

https://t.co/c7wlQOLRiq

Finally, we've added a new label to help identify a cluster of sequences with a S:N501*T* mutation currently circulating in Australia.

This is another independent appearance of a S:N501 (again, to T, not Y) mutation, & is not related to 20C/501Y.V2 or 20B/501Y.V1.

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