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Happy Tuesday! The latest focal S:N501 build is now updated with sequences from 11 Jan.
There are 101 new sequences in the 501Y.V1 (B.1.1.7 #b117) & 501Y.V2 variants.

Additionally, I'll include some updates on S:E484K in this thread.

1/20

https://t.co/dXx6nk6Llz

 There are 97 new non-UK sequences in 501Y.V1, including the first sequences in the cluster from Slovakia, Mexico, & Belgium, plus additional sequences from the Netherlands, Denmark, Australia, Spain, Ireland, & Sweden.

2/20
Slovakia has 10 sequences that fall in 501Y.V1 for the first time. These are closely linked, & likely indicate a single introduction and ongoing transmission (remember: vertical distance is not meaningful on the same branch - position of the 2 English samples is arbitrary).

3/20
Belgium has 5 sequences in 501Y.V1 for the 1st time, indicating 3 separate intros. 3 seqs cluster together (zoomed, divergence), indicating a single introduction. The connection to Netherlands might indicate transmission, or 2 transmissions from same diversity in the UK.

4/20
Mexico also has a sequence that falls into 501Y.V1 for the first time.

5/20
Ireland has 36 new sequences. Many represent separate introductions, but some do cluster with older sequences & may represent local transmission, or introductions from the same UK diversity.

6/20
Denmark has 29 new sequences (orange). These represent both new introductions, and further expansion of the large cluster (zoomed view).

7/20
The Netherlands have 6 new sequences. All fall within the section of the tree marked in red.
Zooming in, the new sequences are marked in red: 2 are separate introductions. Zooming in further (divergence view), cluster at the top are identical to an older sequence.

8/20
Australia has 6 new sequences, including the first from Northern Territory and Victoria. 1 pair of sequences from Victoria are identical to each other, representing one introduction.

9/20
Spain has 2 new sequences (yellow). One represents a separate introduction. The other links to older Spanish sequences, but it's unclear from sequences alone if this is local transmission or transmissions from a common source.

10/20
Sweden has 2 new sequences, marked in red. Both represent independent introductions. The second one is hard to see, but zooming in shows this more clearly.

11/20
Now turning to 501Y.V2:

There are 4 new non-South African sequences in 501Y.V2, including the first from Ireland (3) and the Netherlands (1).

12/20
Ireland has 3 sequences in 501Y.V2 for the first time. They cluster together, and may indicate either local transmission, or a common exposure (pictures 1 & 2).

The Netherlands has its first sequence in 501Y.V2 (picture 3).

13/20
As usual, the updated country graphs are also available. As always, interpret with care: countries are selectively sequencing to track 501Y.V1, so proportions of sequences are not representative for many countries.

14/20

https://t.co/c7wlQOLRiq
Finally, an updated focal S:E484 build is also up.

S:E484K is found predominantly in the 501Y.V2 variant, & in a cluster of sequences primarily in Brazil, though it has appeared numerous times in the past.

15/20

https://t.co/Od95D6DL74
S:E484K has been discussed recently as there are suggestions it may impact how well the immune system can recognise the virus, particularly if you've been infected with SARS-CoV-2 before.

Links to the studies are on https://t.co/SmanZBHpse

16/20
Additionally, over the weekend, travellers from Brazil to Japan were found to be infected with a novel variant with many mutations, including S:E484K & S:N501Y.
Translated report linked below.

17/20

https://t.co/G1ITeT3MoV
To help viewers identify these new samples from Japan, I've added a *temporarily* label to the S:E484K build: 20B/S.484K.501Y.

Additionally, I've added a *temporary* label to the Brazilian cluster with S:E484K: 20B/S.484K.

18/20
Interestingly, though the recent Japanese sequences carry S:E484K & seem to originate from Brazil, they do *not* branch from the larger 484K cluster in Brazil, but instead sit on a long branch separately within the 20B Clade.

19/20
Getting case numbers down saves #LivesAndLivelihoods, and reduces #SARSCoV2's opportunities to explore situations with unique selection pressures, potentially resulting in mutations expanding that we'd rather not see.

Help make my updates more boring: bring cases down!

20/20
PS - For related but slightly-different-angle updates on 501Y.V1 (B.1.1.7) & 501Y.V2 (B.1.351), you should follow @AineToole & keep an eye on her tweets about updated reports!

https://t.co/fZDQqwvJmr

21/20

More from Dr Emma Hodcroft

Dr Emma Hodcroft
Dr Emma Hodcroft
@firefoxx66
The latest focal S:N501 build is now up! It includes data from 4 Jan 2021.

We have new sequences in 501Y.V1/B.1.1.7 from Denmark, Singapore, Australia, Italy, Spain, France, Portugal, Germany, Brazil, Jamaica, Luxembourg, Pakistan & the USA.

1/12

https://t.co/FTjvV7T7wL


Denmark has 43 new sequences (in orange). These indicate additional introductions, as well as further expansion of the existing large local transmission cluster we saw previously (shown in zoomed divergence view).

2/12


10 new sequences from Portugal (orange) indicate additional, separate introductions.

3/12


Singapore has 5 new sequences (yellow). These indicate at least 5 separate introductions for the total of 6 sequences, with two identical sequences likely being 1 introduction.

4/12


5 new sequences from the US, from Florida & California (older sequences crossed through).

Zooming in (divergence view) on the California cluster, the relationship between these samples is difficult to infer from phylogenies alone.

5/12
WORLD
Dr Emma Hodcroft
Dr Emma Hodcroft
@firefoxx66
To add to the excitement of your Saturday evening, the focal S:N501 build is now updated with data from 8th Jan, including 48 new seqs in 501Y.V1 (B.1.1.7 #b117) & 501Y.V2.

1/12

https://t.co/AbdhO1rqpw


There are 46 new non-UK sequences in 501Y.V1, from the Netherlands, Italy, Israel, Ireland, Norway, Sweden, the USA, and Luxembourg.
And 2 new non-South African sequences in 501Y.V2, from Australia for the first time.

2/12


In 501Y.V1:

Ireland has 4 new sequences (orange). 2 sit separately but are identical, indicating an additional introduction. 2 link to a previous sequence (green), 1 mutation away each. This could indicate location transmission, unsampled diversity, or a common exposure.

3/12


The Netherlands has 10 new seqs (orange). Many sit apart on the tree, indicating additional introductions. 3 cluster together separated by a few mutations. 2 are identical to an older seq. 1 falls in a cluster of older seqs. Some of these could indicate local transmission.

4/12


Israel has 22 new sequences (orange). 1 links to an older sequence, but is separated by 3 mutations, so may be separate introductions. Another is identical to an older sequence. But most new sequences cluster together (blue), clearly indicating local transmission.

5/12
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Dr Emma Hodcroft
Dr Emma Hodcroft
@firefoxx66
The focal S:N501 build is now updated with data from 13 Jan!

There are a total of 247 non-UK & non-South African sequences in 501Y.V1 (B.1.1.7 #b117) & 501Y.V2.

I'll do an additional thread later on, covering S:E484 & the 'Ohio variants'.

1/18

https://t.co/3bdttjKVl4


There are 244 new sequences in 501Y.V1 (B.1.1.7) from Australia, Belgium, Denmark, Germany, India, Israel, Italy, Netherlands, New Zealand, Norway, Portugal, Singapore, Spain & USA, as well sequences as from Ecuador and Greece for the first time.

2/18


Greece has 3 new sequences in 501Y.V1 for the first time. They indicate 2 separate introductions.

Ecuador has 2 sequences for the first time. They also indicate 2 separate introductions.

3/18


The USA has 61 new sequences (orange), from Florida, Minnesota, California, Pennsylvania, & New York.

A few of these represent separate introductions. Others form distinct clusters that may indicate ongoing local transmission.

4/18


Israel has 35 new sequences (orange). Some of these show separate introductions, but many link with older sequences, indicating ongoing local transmission.

5/18
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Dr Emma Hodcroft
Dr Emma Hodcroft
@firefoxx66
The S:N501 build is now updated with data from 25th Jan.

Remember, you can always check on any country by just typing that country name in the 'Filter' box on the left menu!

1/18

https://t.co/FTjvV7T7wL


In the 501Y.V1 (B.1.1.7) variant, here are a few of the non-UK countries with new sequences in this variant, including Denmark, Italy, Spain, France, Portugal, Norway, Germany, the USA, South Kora, & Switzerland, & the UAE, Austria, & Czech Republic for the 1st time

2/18


Denmark has 52 new samples (some yellow), showing both separate introductions and further spread of existing local clusters.

3/18


Spain has 62 new sequences (orange & yellow). Many of these are separate introductions, and a few new sequences form distinct clusters. Some new sequences also cluster closely with older sequences (green).

4/18


France has 72 new sequences (orange & yellow). These show both separate introductions and clustering with older sequences.

5/18
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Dr Emma Hodcroft
Dr Emma Hodcroft
@firefoxx66
A brief update to the focal S:N501 builds to start your Friday off right! This one includes data from 7 Jan 21.

We have about 20 new sequences each in 501Y.V1 (B.1.1.7 #b117) & 501Y.V2.

Let's take a look at the new sequences, starting with 501Y.V1

1/14

https://t.co/kT1gFsSZn0


There are 22 new non-UK sequences in 501Y.V1, including for the first time from Luxembourg (3), & additional seqs from Singapore (1), Australia (4), France (12), & Switzerland (2).

2/14


First, Luxembourg has 3 sequences in 501Y.V1 for the first time. Each represents a separate introduction.

3/14


France has 12 new sequences (orange). Many of these represent independent introductions.

Zooming in (divergence view), we can see 2 new sequences are identical (possible local transmission), & 2 differ by 2 mutations (1 each).

4/14


Australia has 4 new sequences (red and orange) which are spread across the tree, representing separate additional introductions.

5/14
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\U0001f1fa\U0001f1f8 Jules \U0001f1fa\U0001f1f8
🇺🇸 Jules 🇺🇸...
@mayweliveasONE
2021 Predictions‼️

Made by James Howard Kunstler

👇🏻👀

Kunstler is said to be one of the best thinkers and authors of our modern era. He’s well known as an expert in energy economics, among other topics.

He is NOT a fan of Trump which makes his predictions even more interesting.

1) The election is re-adjudicated, fraud subtracted from the tally, and President Trump is declared the winner.

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4) The CIA is purged and reduced to a strictly analytical role for advising the executive.
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Mau Abata \U0001314a
Mau Abata 𓅊
@MauAbata
Read through a forum thread on Chastity Mansion to see who is talking about the Edge-o-Matic and figure out what more to address regarding frequent questions, as well as scout out user feedback. Here's some thoughts you can carry back to the forums!

My website doesn't have any product reviews, but people scatter them everywhere else in forums, tweets, xtube vids, etc.

If you ARE a previous customer, and you like the thing, can you drop a review on my site to help others?

The price is steep, but that's the amount of coins I calculated to make these. I have several spreadsheets calculating every expense involved with this project.

The current cost sustains production and development of new tech, and is helping create jobs. We are 2 people now!!

It IS 3D printed, because injection molding is orders of magnitude more expensive for the initial tooling, and I am still making small revisions to the enclosures.

Injection molded plastics are a six thousand dollar commitment for fifteen cent parts. Great for mass mfg.

Also, if you didn't know, 3D printing technology allows for some shapes which would be difficult, expensive, or impossible on injection molding or milled parts!!

Undercuts, which I use in this design, require special lifters to mold that cost a tremendous fortune.
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Thinking Patriot
Thinking Patriot
@ThinkinPatriot
1⃣
At the moment #POTUS is sitting on a stack of #Trump cards, he's just waiting to unleash a royal flush!
He has court cases that will go to #SCOTUS and thanks to the Texas case, he's now aware how to file them properly under article 3 not 2, so SCOTUS will be forced to listen.

2⃣
#Trump now has the #DNI report. #Barr stepped down and can now be a witness, he did his job. #Durham is special counsel and can prosecute, in any state.
He’s letting civil, criminal and federal courts fail to handle the situation properly so he can use military tribunals.

3⃣
#Trump has ALL the data from the #NSA, the #Kraken supercomputer, the #Alice #supercomputer, and likely many more computers, unknown to us.
He has the dueling electors from 7 state legislatures. He has VP #Pence, as the final arbiter of which ballots to accept.

4⃣
#Trump has the #InsurrectionAct, the #NDAA, the national emergency, the 14th amendment, the 2018 executive order, the 2017 very first EO, the #Patriot Act, the #FISA warrants, the Declassification of everything, people swearing affidavits by the 1000s.

5⃣
#Trump has all the statistical data being analyzed along with the videos, emails, phone calls and bank transfer statements showing the coordination of the #coup d'etat.
He has #RICO and he has the #CrimesAgainstHumanity videos. #Wikileaks just dropped extensive information
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Jo Luehmann
Jo Luehmann
@JoLuehmann
Christianity has been a weapon used by systems of oppression to call that which is harmful and abusive; good and God’s will. It has been a weapon to normalize hegemonic power, and keep people from questioning it or challenging it because “it comes from God.”

1/

Gender “norms,” harmful ideas about sexuality, social hierarchy, the construct of race, notions of nuclear family, toxic ideologies of work, bodies, finances, government, relationships, parenting, or criminal justice; are all rooted in Christianity and...

2/

backed up by verses of the Christian Bible taken out of context, to assert that this isn’t just normal, but what God desires. And who dares challenge God?

Christianity has allowed for people w/power to use the idea of god, and *his* “word,” to pass their ideas as God ideas.
3/

This is one of the reasons why people react so violently when we say the Bible is not the word of God, they can’t accept that everything they’ve been given as normal, good, ideal, God’s will... may just not be so.

4/

Their nervous systems tells them it is better to maintain the certainty of what is in their hands; even though it causes harm; than to give into the uncertainty of imagining a different world where equity is possible.

And then their ego gets involved, and admitting they’ve

5/
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Boz Tchividjian
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@BozT
The evils committed by Ravi Zacharias were enabled by a toxic organizational leadership that is evidenced by how these same leaders responded when confronted about the widespread and ongoing abuses being perpetrated by the namesake of the institution. 1/29

Please take time to read this piece by @DavideAFrench and the 26-page letter written by a first-hand witness. Here are just some of the excerpts: 2/29

"At one point, Ravi suggested to me that the Task Force “issue a statement that they [the Thompsons] are hooligans, they are scoundrels, they are thugs, they are serial extortioners.” If we didn’t do that, he told me, he would ask his attorney to do so on his behalf." 3/29

"...I distinctly recall Ravi & Sanj mocking the Thompsons’ marriage & scornfully predicting how soon Brad & Lori Anne would get divorced. “I predict their marriage won’t survive the year,” Ravi asserted with laughter. “A year?” replied Sanj. “I wouldn’t give it six months!” 4/29

"In 2017, in the early stages of his RICO against the Thompsons, Ravi would often invoke Kelly [his attorney], making statements such as “my attorney said we are going to crush them.”" 5/29
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🎉🎉 PHP 7.3 is here!

Here are the things I'm excited about.

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MINUTE DETAILS ABOUT THE ATTACK ON SHAISTA KHAN AT LAL MAHAL

Remarkable Commando op

A long 🧵 but worth your time

Aurangzeb sent his maternal uncle Shaista Khan with powerful army over 1,50,000 on the request of Badibegum Sahiba, Adilshahi sultanate

1/


Shaista Khan entered Deccan to kill Chhatrapati Shivaji Maharaj & to destroy his empire. Shahista Khan captured a lot of province and fort of Chakan near Pune.

2/


Till 3 years he gave lot of troubles to the subjects of Chhatrapati Shivaji Maharaj and he personally made Lal Mahal his destination to live at , which was the property of Chhatrapati Shivaji Maharaj . Lal Mahal, where Chhatrapati Shivaji Maharaj spent his childhood.

3/

1. In the April 1663 Chhatrapati Shivaji Maharaj personally made a surprise attack on Shahista Khan in the Lal Mahal Pune.

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Anu Satheesh \U0001f1ee\U0001f1f3
Anu Satheesh 🇮🇳...
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Cherthala Karthyayani Kshetram
#Alappuzha, Kerala
#KeralaTemples 🚩

Cherthala Karthyayani Temple Prathishta was done by Vilwamangalam Swamy. Chertala Pooram is very famous. Devi is Mangalya Dayini here removes obstacles relating to marriages. Sthala Puranam says that, once 👇


Vilwamangalam Swamiyar was passing this area after Prathista of Padmananbhaswamy Temple, he felt Devi chaitanyam and recognized as Karthyayani Devi. But Devi seeing Vilwamangalam went inside the pond. Swamy searched for Devi continously for 6 days and on 7th day Vilwamangalam


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