Let Uncle Ralph educate you
Synthetic Viral Genomics:
Risks and Benefits for Science and Society

These enzymes can be used to create unique interconnecting junctions, which can be subsequently removed from final assembly product allowing seamless reconstruction of an exact sequence




The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
This powerful technique provides bioterrorists with a “scapegoat” option; leaving a sequence signature that misdirects efforts at tracking the true originators of the crime. Even better, the approach could be used to build mistrust &/or precipitate open warfare

Hence Ecohealth DARPA/DTRA spooks & virus thieves collaboration with Baric (UNC) Lipkin (Mailman) NIchols (Atlanta CDC) and USAMRIID (Bavari, Totura et al) & Jonathan Epstein's palpable concern about dual use references in the @USRightToKnow FOIA emails

Delving into the cold and calculating mind of a twisted genius?
https://t.co/MrwxYK93cx
Bring Uncle Ralph and his transgenic mice in for questioning!
unroll @threadreaderapp

https://t.co/rjNZ8aDNDl

1. Synthetic Genomics: Options for Governance (2008) https://t.co/A32jEfL1TQ
2. Sequence Screening - Robert Jones (2005)
https://t.co/zOqMroYbVU
3. Synthetic Biology as a Field of Dual-Use Bioethical Concern - Alexander Kelle
https://t.co/Va1movPqvs
4. Sanghvi Y. A Roadmap to the Assembly of Synthetic DNA from Raw Materials.
https://t.co/4kWu4vzcXC
5. Collett MS. Impact of Synthetic Genomics on the Threat of Bioterrorism with Viral Agents.
https://t.co/OcaTmuCUBy
6. Fleming DO. Risk Assessment of Synthetic Genomics: A Biosafety & Biosecurity Perspective.
https://t.co/JBcLVLr005
7. Risk Governance of Synthetic Biology
https://t.co/9BE9X5s0Fb
8. US Competitiveness in Synthetic Biology
https://t.co/OCZJdsXSjl
9. Ensuring security of synthetic biology
https://t.co/7kPE1OpbrS
10. Synthetic biology: emerging research field in China
https://t.co/T3BcaDvUsa
11.
What rough beast? Synthetic biology, uncertainty,& the future of biosecurity (2016)
https://t.co/XTlBXILEWh

Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus (2003)
https://t.co/k8PI1rbcm8
Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 (2008)
https://t.co/bWhAv1wsC0

In 2016, Shi and her team at the WIV, in conjunction with the New York-based EcoHealth Alliance, constructed a full-length clone of a bat coronavirus called SL-CoV WIV1. They assembled it in discrete segments.
“Thus, several options exist to remove the desired insert DNA with a single restriction digestion.”
This shows that researchers at the WIV have the ability to genetically engineer viruses and remove the signatures of the genetic engineering.
They showed how they can insert new spikes into viruses. The researchers state:
“Then any spike could be substituted into the genome of SARSr-CoV WIV1 through this strategy.”
COVID 19: The Spike and the Furin Cleavage
https://t.co/bDOMmhpJ1t
More from Billy Bostickson 🏴👁&👁 🆓
41,000 mutations documented in 880 lineages.
Most in spike protein
What explains it?

https://t.co/MvHzUb4peo
1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice https://t.co/TNE4tuLkqN
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
https://t.co/PAwgC3lgsO
2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2 https://t.co/B3eR764c1Z
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
https://t.co/f9rnyFI0vq
1. Reversion of molecularly engineered, partially attenuated, very virulent infectious bursal disease virus during infection of commercial chickenshttps://t.co/wOg1Ekl3Xp
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
2. Reversion to Virulence of Attenuated Canine Distemper Virus In Vivo and In Vitrohttps://t.co/xhjOEYEWTY
https://t.co/wAlyNzuI0W
3. pathogenic reversion of simian immunodeficiency virus SIVmac239Deltanef increases viral replicationhttps://t.co/jHmujgDVAC
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
A, Wuhan Seafood Market Positive Environmental Samples, B. Early Local Reports about Animals at the Market
C. CCP Censorship and Police harassment of local activists uploading censored news reports to GitHub.
The original thread was a mess!
2. CCP Censorship & Police harassment of local activists uploading censored news reports to GitHub.
It all started with a simple search query:
"market" "animal" on a github database:
https://t.co/q2HxuUuHKa
Only to find that it had been deleted on github due to CCP pressure!
3. Github is Censorship proof, right?
It does not get deleted! But..
https://t.co/k7BJWUgWDq
Arms get Twisted!
https://t.co/ckzicj7NlE
Please remember these brave young activists for creating a permanent record of censored news from the early days while CCP rewrites history

4. What a shock!
But thanks to the very nature of GitHub, "forks" and "clones" had been made & saved.
The best one is here:
https://t.co/ZWiyWK9SAa
Obviously all in Chinese ;) but tks to Google Chrome we can right click translate to English & find archived scrubbed Chinese news

5. "This is an archive of in-depth reports and personal narratives on the COVID-19 outbreak in China"
Contains media reports, non-fictional works and personal narratives related to the 2020 coronavirus pneumonia.
Part of the archive is provided by netizens, I am grateful.
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.
The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains
(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK

In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa

Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs

Document:
https://t.co/M9cgCOXFsP

2. Supporting Documents
3. Supporting Documents
4. Supporting Documents
5. Supporting Documents (4)
https://t.co/5OVM8F3huO
and
https://t.co/g1tOkKYVCK
Confirmed:
https://t.co/7Mm9LuyoT1
Disputed based on doctored soviet papers by Milton
More from Science
https://t.co/mzS7vVSREJ
https://t.co/353PdAX2fa
https://t.co/3yBImjOdd4
In some cases, almost 100% of the light energy can be converted to the second harmonic frequency. These cases typically involve intense pulsed laser beams passing through large crystals, and careful alignment to obtain phase matching.

The physicist Hugh Everett III was born #OTD in 1930. His \u201crelative state\u201d formulation of quantum mechanics, which we now call the \u201cMany Worlds Interpretation,\u201d was published in 1957. pic.twitter.com/ZqMsZcPJDG
— Robert McNees, the bastegod (@mcnees) November 11, 2020
We look at the night sky and see photons arriving to us, emitted by distant stars. Let's contrast two different theories about how stars emit photons.
One theory says, we know how stars shine, and our equations predict that they emit photons roughly uniformly in all directions. Call this the "Many-Photons Interpretation" (MPI).
But! Others object. That is *so many photons*. Most of which we don't observe, and can't observe, since they're moving away at the speed of light. It's too ontologically extravagant to posit a huge number of unobservable things!
So they suggest a "Photon Collapse Interpretation." According to this theory, the photons emitted toward us actually exist. But photons that would be emitted in directions we will never observe simply collapse into utter non-existence.
😭
The new answer to a 77-year-old problem in data analysis, published today in @naturemethods. Instead of significance tests, use estimation graphics. Our software suite DABEST makes it easy for everyone to visualize effect sizes.https://t.co/UzwXJ7EUC5 pic.twitter.com/VtxyY0xaRM
— Adam Claridge-Chang (@adamcchang) June 19, 2019
https://t.co/hm9NoaU4nr
Open letter to journal editors: dynamite plots must die. Dynamite plots, also known as bar and line graphs, hide important information. Editors should require authors to show readers the data and avoid these plots. https://t.co/0GNKEIUCJL pic.twitter.com/OS9ytEFRZN
— Rafael Irizarry (@rafalab) February 22, 2019
https://t.co/8fKDiKjSWc
Couldn't find D3 code for grouped horisontal box plots that show data points so I made this @mbostock @thisisalfie https://t.co/cQjDPhyZdw pic.twitter.com/y6RNmDB2p3
— Ulrik Lyngs (@ulyngs) June 28, 2017
https://t.co/jkaicC1F2x
made a pkg for pirate plots in ggplot: add any of points/means/bars/CIs/violins \u2013 better than ye olde bar/box plotshttps://t.co/Z2m2kW3hsl pic.twitter.com/npAirPQexM
— Mika Braginsky (@mbraginsky) September 28, 2017
https://t.co/PpxWT4Jef4
See the new #PowerBI visual awesomeness for data points & sources, box-&-whisker plots! https://t.co/dOmgoxWfDE pic.twitter.com/HAUOAMJEJW
— Microsoft Power BI (@MSPowerBI) February 1, 2016