1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice https://t.co/TNE4tuLkqN
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
Same covid-19 mutations are appearing in different places, which may or may not be strange....
41,000 mutations documented in 880 lineages.
Most in spike protein
What explains it?
2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2 https://t.co/B3eR764c1Z
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
1. Reversion of molecularly engineered, partially attenuated, very virulent infectious bursal disease virus during infection of commercial chickenshttps://t.co/wOg1Ekl3Xp
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
2. Reversion to Virulence of Attenuated Canine Distemper Virus In Vivo and In Vitrohttps://t.co/xhjOEYEWTY
3. pathogenic reversion of simian immunodeficiency virus SIVmac239Deltanef increases viral replicationhttps://t.co/jHmujgDVAC
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
4. Genetic suppression in
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
reovirus: Ramig et al. 1977
papovavirus: Shortle et al. 1979
picornavirus: King et al. 1980
vaccinia virus: McFadden et al. 1980
influenza virus: Tolpin et al. 1981
herpesvirus: Hall and Almey 1982
adenovirus: Kruijer et al. 1983https://t.co/4zARJZWBzk
5. reversion to virulence of live attenuated vaccines
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
1. Evolutionary reversion of live viral vaccines: Can genetic engineering subdue it?https://t.co/9lY6ji88Gr
2. Mechanisms Causing Reversion to Virulence in an Attenuated SARS-CoVhttps://t.co/ogvKaqiQj8
6. reversion to virulence of live attenuated vaccines
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
3. The double-edged sword: How evolution can make or break a live-attenuated virus vaccinehttps://t.co/jRJNTAR6tn
4. Selection Versus Mutation: Reducing the Risk of Vaccine Reversionhttps://t.co/dkVs3wTkve
7. NSP16 mutant reverts in immune-compromised model
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021
and SARS-CoV dNSP16 can revert to virulence
Menachery, Yount & Baric 2018
Combination attenuation offers strategy for live-attenuated coronavirus vaccineshttps://t.co/q2oQQzc1SG pic.twitter.com/RhnBtrGhVW
Was sars-cov-2 or its progenitor adapted to hACE2 during serial passage in human xenograft lung-transplanted mice at WIV?
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 20, 2021
We know that several mink variants and B.1.1.7 are well adapted to murine ACE2.
This may imply reversion of sars-cov-2 to a murine adapted progenitor.
Just to annoy people
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
Virology: the problem with \u2018leaky\u2019 vaccines
Fresh evidence supports the theory that some vaccines lead to the evolution of more virulent viral strainshttps://t.co/ttyM9J2idI
B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.https://t.co/9Z4oJmkcKj pic.twitter.com/1Q65DpHboK
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
Mice!
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
A mink-associated variant carrying \u0394(69-70)-Y453F-F486L-N501T-M1229I mutations was also able to utilize mouse Ace2. In addition, all variants carrying an N501Y mutation, a shared feature of UK, South Africa, & Brazil VOC strains, efficiently use mouse Ace2 orthologs.
Topolino!
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
"Moreover, the K417N-E484K-N501Y mutations found in the South Africa variant 501Y.V2 even enable the virus to utilize rat Ace2 more efficiently than using human ACE2. These data suggest that rats and mice may (have) be(en) able to harbor and spread these variants"? pic.twitter.com/kC3dVwmTyg
Above taken from:
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
Circulating SARS-CoV-2 variants B.1.1.7, 501Y.V2, and P.1 have gained ability to utilize rat and mouse Ace2 and altered in vitro sensitivity to neutralizing antibodies and ACE2-Ighttps://t.co/j9qEcUz4ea
Well worth a read! pic.twitter.com/w6VLjhpjf4
even the mink variants were mouse ACE2 adapted, what a surprise...now, who would have expected that? pic.twitter.com/AVPwXeJ1tT
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021
More from Billy Bostickson 🏴👁&👁 🆓
Another look at some key people in the GVP & their media statements since January 2020 about "natural origin" and impossibility of a "lab leak"
Full List of 18 scientists in photograph is here:
https://t.co/yHoAjwQjI8
2/X
We have already looked closely at Dennis Carroll, Peter Daszak, George Gao, W. Ian Lipkin & seen their public statements supporting "natural origin" & hysterical attacks on "lab leak" theories.
This thread focuses on:
Michael Kurilla
Jonna Mazet
Edward Rubin (Metabiota)
3/X
If we look at the leadership team of GVP, you will see some familiar faces:
https://t.co/WdT8sNnfmy
Most familiar is probably Peter Daszak, the wealthy Treasurer, also with the key role of Secretary.
Note: USAID PREDICT Project - EcoHealthAlliance
4/X
The "Chair" is Dr. Dennis Carroll
We will have a look at his media statements on the coronavirus pandemic by inputting his name in Twitter search box (very easy!)
Note: Previously was Director of USAID Emerging Pandemic Threats Program
5/X Dennis Carroll's video media statements (1):
CNN
(note Qinghai Thangka positioned in background which Daszak also shows off in his videos obtained during EcoHealth virus hunt with
Warnings ignored, and the stunning fact that the wealthiest country in the world is short of masks and gowns and ventilators - and possibly, hospital beds. Dr Dennis Carroll says it's time to rip off the bandaid on harsh #coronavirus\xa0measures. #COVID19 pic.twitter.com/ZNZnhZnmPs
— Michael Holmes (@holmescnn) March 21, 2020
2 papers:
Human–viral molecular mimicry
https://t.co/irfH0Zgrve
Molecular Mimicry
https://t.co/yLQoUtfS6s
2. Must Read! Confirmation with supporting evidence via @flavinkins
https://t.co/NEf9HK6ReB
"ONLY place where this thing could emerge, naturally or Artificially, is by passage in HUMANIZED MICE"
3. Springer Article
The PEPTIDES again!
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) October 21, 2020
"Such a peptide commonality is unexpected and highly improbable from a mathematical point of view"
Molecular mimicry between SARS-CoV-2 spike glycoprotein and mammalian proteomes: implications for the vaccinehttps://t.co/yLQoUtxtv2
via @gerdosi https://t.co/lsCv2j4gf9 pic.twitter.com/hdgM5KZd39
4. Takeaway most significant quotes from Springer Article, burn this into your brain:
"Such a peptide commonality is unexpected and highly improbable from a mathematical point of view"
5. One image to explain it all!
International Training Course on Biosafety Laboratory Management and Technology
1. Daniel Feakes, head of UN Biological Weapons Convention implementation support agency
2. René Courcol, quality control system expert, France's BSL
List of Lecturers with short bios at
November 3 - 9, WIV Training Course: https://t.co/Bsd9yO40JP
YUAN Zhiming Professor, Wuhan Institute of Virology, CAS (Deputy Director)
SONG Donglin, Professor, Wuhan Institute of Virology, CAS ( Deputy Director of Wuhan BSL-4 Laboratory.)
Dr. TONG Xiao Senior Engineer at WIV (Molecular Virology & (HIV) infection, mechanical & vaccine immunology) On-site Manager & Chief engineer for WIV BSL-4 Lab construction, maintenance & biosafety equipment R&D.
Zhengli Shi, Director of WIV Center for Emerging Infectious Diseases, now in charge of scientific activity in BSL3 & BSL4. Viral pathogen discovery through sequencing techniques, wildlife-borne viral pathogens, particularly bat-borne viruses since 2004.
DENG Fei, Principle Investigator in Research Group on Viruses Resources & Biotechnology in WIV, also Director of Microorganisms & Viruses Resource Center.
Graduate of ECUST, went to Great Lakes Forestry Center, Canada in 2001 & Wageningen University, NL to study baculovirus.
dsRNA viruses molecular biology
https://t.co/lwrQoo6ygG
2. Emerging Viruses Group
https://t.co/ND56gVGOAn
3. Structural Biology of Viral Genome Replication
https://t.co/wUqc0YIP3X
4. Molecular Biology of hepatitis Viruses & Gene Therapy
https://t.co/snBbxkhNsC
5. Insect Virus Genetic Engineering Lab
https://t.co/S68mVOA2Ob
More from All
https://t.co/VUYbsKGncx
Kristian G. Andersen
Andrew Rambaut
Ian Lipkin
Edward C. Holmes
Robert F. Garry
2. Thanks to @newboxer007 for forwarding the link to the research by an Australian in Taiwan (not on
3. K.Andersen didn't mention "competing interests"
Only Garry listed Zalgen Labs, which we will look at later.
In acknowledgements, Michael Farzan, Wellcome Trust, NIH, ERC & ARC are mentioned.
Author affiliations listed as usual.
Note the 328 Citations!
https://t.co/nmOeohM89Q
4. Kristian Andersen (1)
Andersen worked with USAMRIID & Fort Detrick scientists on research, with Robert Garry, Jens Kuhn & Sina Bavari among
Our Hans Kristian Andersen working with Jens H. Kuhn, Sina Bavari, Robert F. Garry, Stuart T. Nichol,Gustavo Palacios, Sheli R. Radoshitzky from USAMRIID and Fort Detrick to tell more fairy tales? Full emails listed for queries...https://t.co/kLRoQTxiGD pic.twitter.com/uHNuGraPP2
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) August 26, 2020
5. Kristian Andersen (2)
Works at Scripps Research Institute, which WAS in serious financial trouble, haemorrhaging 20 million $ a year.
But just when the first virus cases were emerging, they received great news.
They issued a press release dated November 27, 2019: