Keep dwelling on this:
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.
https://t.co/EwwJOSa8RK
https://t.co/BsY8BAIzDa
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs
Keep on Puffing, Ignore the Guys in White Coats!
https://t.co/EWmVWI5jYr
Anyway, this is where Cheng et al (2020) got the neurotoxin motifs from
Superantigenic character of insert unique to SARS-CoV-2
https://t.co/EwwJOSa8RK
Bracci L., Ballas S. K., Spreafico A., Neri P., Molecular mimicry between the rabies virus glycoprotein and human immunodeficiency virus-1 GP120: cross-reacting antibodies induced by rabies vaccination.
https://t.co/UZbe658OGR
(apart from being good news for smokers...)
Maybe someone else can unravel the implications?
@Parsifaler @w_mccairn @Rossana38510044
"For binding experiments, cells were harvested mechanically with a rubber policeman & centrifuged"
Binding of HIV-1 gp120 to the nicotinic receptor (1992)
https://t.co/6veoEkVbVp
Referenced in Bracci et al (1997)
https://t.co/UZbe658OGR
Sequence homology between HIV gp120, rabies virus glycoprotein, and snake venom neurotoxins - Is the nicotinic acetylcholine receptor an HIV receptor?
https://t.co/XGVUNyORP3
(Sönnerborg & Johansson, 1993)
The neurotoxin-like sequence of human immunodeficiency virus GP120: A comparison of sequence data from patients with and without neurological symptoms
https://t.co/s8DIeMNMmD
1. Molecular mimicry of HIV gp120 (2005) https://t.co/KzfEpbjbvk
2. McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
https://t.co/iG8NSrGrlI
3. Snake envenomation https://t.co/uJy1bi7NEU
4. Modified venom and venom components as anti-retroviral agents (PATENT)
https://t.co/VRH0gJlbqM
5. Recombinant Newcastle disease virus (NDV) strain rL-RVG expressing the rabies virus glycoprotein
https://t.co/31SNcPXNYJ
6. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein
https://t.co/Ce9cAEOCQH
Role of HIV gp120 in crossing blood brain barrier
Interactions of SARS-CoV-2 with the Blood–Brain Barrier
https://t.co/oZWRqn5cGr
HIV-1 protein gp120 crosses the blood-brain barrier role of adsorptive endocytosis
https://t.co/9pdkqSt0xz
Final Thesis Jack Sim
In-vitro Characterisation of Targeting Ligands for
Enhanced Delivery Across the Blood-Brain Barrier
https://t.co/GAURhTA376
https://t.co/XQ71CTh93k
Simulations support the interaction of the SARS-CoV-2 spike protein with nicotinic acetylcholine receptors and suggest subtype specificity
https://t.co/umDn3S549X
Simulations support the interaction of the SARS-CoV-2 spike protein with nicotinic acetylcholine receptors
https://t.co/1Qg0tKg3YI
Media Review:
https://t.co/2yyYJw3zVv
2. SARS-CoV-2 spike may act as a superantigen to trigger the development of MIS-C as well as cytokine storm in adult COVID-19 patients
https://t.co/sXQyd7kMx5
COVID-19–associated multisystem inflammatory syndrome in children (MIS-C): A novel disease that mimics toxic shock syndrome—the superantigen hypothesis
https://t.co/NxmPM719VB
https://t.co/b8PssOG6my
SEB confirmed again
1. What is Happening with Smokers and COVID-19?
https://t.co/wXGYU9EjAp
2. Cytokine Release Syndrome (CRS) and Nicotine in COVID-19 Patients
https://t.co/3oA9sWOTaU
and
https://t.co/4yGz47igy2
3. Involvement of nicotine receptors in COVID-19
https://t.co/0GtJwhu9tU
Who put the Benzedrine in Mrs Murphy's Ovaltine?
Who put SAg & SEB in SARS COV-2's Spike Protein?
Note:
A monoclonal antibody against staphylococcal enterotoxin B superantigen inhibits SARS-CoV-2 entry
https://t.co/x7iAVt4Zvm
But a solution is proposed...
A monoclonal antibody against staphylococcal enterotoxin B superantigen inhibits SARS-CoV-2 entry in vitro
https://t.co/x7iAVt4Zvm
and
https://t.co/NgZ7aNd2KS
Molecular mimicry between rabies virus glycoprotein & HIV-1 GP120
https://t.co/Ahp5mE27uI
A Novel Vaccine Employing Non-Replicating Rabies Virus Expressing Chimeric SARS-CoV-2 Spike Protein Domains: Functional Inhibition of Viral/Nicotinic Acetylcholine Receptor Complexes
https://t.co/Zp6gHLPE1x
Persistent SARS-2 infections contribute to long COVID-19
https://t.co/R5UI7pOynJ
For the Curious:
Dampened Bat Immune System Interferon Response?
Why COVID-19 Transmission Is More Efficient and Aggressive Than Viral Transmission in Previous Coronavirus Epidemics?
https://t.co/yNTOP9Lf37
Good Luck with finding the answers!
https://t.co/9iQQBiUjaS
References 145, 146 & 147 for the curious:
https://t.co/yNTOP9Lf37
Host resistance influences patterns of experimental viral adaptation
https://t.co/gnLm32z0G6
More from Billy Bostickson 🏴👁&👁 🆓
@snip96581187 @Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen Clearly, because as I have been saying for 8 months now, DTRA and DARPA have been using Ecohealth and UC Davis to collect novel pathogens for gain of function work back in the USA. I have documented this in many threads which I will post here just to annoy everyone.
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
28. Before moving on to DARPA, let's look at DTRA:
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) July 31, 2020
A must read!
It is astonishing the number of pies they had their dirty little fingers poking into:
Note John Epstein and Kevin Olival from EcoHealth Alliance are key figures in DTRA:https://t.co/O4QwVWrm7m pic.twitter.com/cnNGZ7AApj
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
24. DTRA Network for Collection of Viruses
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 9, 2021
7. DTRA - Metabiota - One Health - Ecohealth
Bat Research Networks and Viral Surveillance: Gaps and Opportunities in Western Asia pic.twitter.com/SOqSSXF3pa
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
That is the key question
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 5, 2021
1. DARPA/DTRA use NGOs like Ecohealth or Metabiota to collect new pathogens
2. They are sent to US labs (Mailman, Rocky Mountain, Atlanta CDC, UNC, USAMRIID) for GOF work by Lipkin, Nichols, Rasmussen, Baric, Dension, Munster, etchttps://t.co/wqhHK7uZO6
@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
1. I wonder why Dr. Angela Rasmussen is so so upset & full of almost palpable venom about a Hypothesis and a "What if" question by @nicholsonbaker8 in the @NYMag https://t.co/a6lxtJLpKR
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 5, 2021
Did I hear someone say "DARPA"?
Did I hear someone say "DTRA"?https://t.co/i27mpxJDw2 pic.twitter.com/x4X3QPnTMS
@EricTopol @NBA @StephenKissler @yhgrad B.1.1.7 reveals clearly that SARS-CoV-2 is reverting to its original pre-outbreak condition, i.e. adapted to transgenic hACE2 mice (either Baric's BALB/c ones or others used at WIV labs during chimeric bat coronavirus experiments aimed at developing a pan betacoronavirus vaccine)
@NBA @StephenKissler @yhgrad 1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice
@NBA @StephenKissler @yhgrad 2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2
@NBA @StephenKissler @yhgrad B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.
https://t.co/9Z4oJmkcKj
@NBA @StephenKissler @yhgrad We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the ... Thus, this mouse-adapted strain and associated challenge model should be ... (B) SARS-CoV-2 genomic RNA loads in mouse lung homogenates at P0 to P6.
https://t.co/I90OOCJg7o
@NBA @StephenKissler @yhgrad 1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice
1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 30, 2021
"we generated a mouse model expressing hACE2 by using CRISPR/Cas9 knockin technology. In comparison with wild-type C57BL/6 mice, both young & aged hACE2 mice sustained high viral loads... pic.twitter.com/j94XtSkscj
@NBA @StephenKissler @yhgrad 2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2
1. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2!
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 2, 2021
2 papers:
Human\u2013viral molecular mimicryhttps://t.co/irfH0Zgrve
Molecular Mimicryhttps://t.co/yLQoUtfS6s https://t.co/lsCv2iMEQz
@NBA @StephenKissler @yhgrad B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.
https://t.co/9Z4oJmkcKj
@NBA @StephenKissler @yhgrad We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the ... Thus, this mouse-adapted strain and associated challenge model should be ... (B) SARS-CoV-2 genomic RNA loads in mouse lung homogenates at P0 to P6.
https://t.co/I90OOCJg7o
1/10 What's all this then?
Biotech or biohazard?
A proposed federal lab in Butner would study the world's deadliest diseases
https://t.co/krnmueAd80
is it being built there or elsewhere, at Kansas University?
2/10 Shades of Plum Island?
New Proposal: https://t.co/Lv3FgBgyas
GAO reports on security and safety lapses at Plum Island Animal Disease Research Center
Plum Island 1: https://t.co/pXSHgg1KKN
Plum Island 2:
https://t.co/O9oS0WXYOz
3/10 National Bio and Agro-Defense Facility
By memo dated December 4, 2008, the Department of Homeland Security named the Kansas site as the preferred location for the NBAF. On January 16, 2009, the record of decision was published in the Federal
4/10 Lucky ole Kansas!
The $1.25 billion facility is a biosafety level-4 laboratory and will replace the aging Plum Island Animal Disease Center.
NBAF is expected to be operational by 2022-2023.
Manhattan, Kansas, was selected as the location for NBAF.
https://t.co/kiokkFsT6v
5/10 The facility continues forward - Apr 19, 2020
https://t.co/JlstInKIRS
https://t.co/DtbLD9Tvxb
The 500,000 square foot building will have a $3.5 billion impact on the Kansas economy and many lab
Biotech or biohazard?
A proposed federal lab in Butner would study the world's deadliest diseases
https://t.co/krnmueAd80
is it being built there or elsewhere, at Kansas University?
2/10 Shades of Plum Island?
New Proposal: https://t.co/Lv3FgBgyas
GAO reports on security and safety lapses at Plum Island Animal Disease Research Center
Plum Island 1: https://t.co/pXSHgg1KKN
Plum Island 2:
https://t.co/O9oS0WXYOz
3/10 National Bio and Agro-Defense Facility
By memo dated December 4, 2008, the Department of Homeland Security named the Kansas site as the preferred location for the NBAF. On January 16, 2009, the record of decision was published in the Federal
4/10 Lucky ole Kansas!
The $1.25 billion facility is a biosafety level-4 laboratory and will replace the aging Plum Island Animal Disease Center.
NBAF is expected to be operational by 2022-2023.
Manhattan, Kansas, was selected as the location for NBAF.
https://t.co/kiokkFsT6v
5/10 The facility continues forward - Apr 19, 2020
https://t.co/JlstInKIRS
https://t.co/DtbLD9Tvxb
The 500,000 square foot building will have a $3.5 billion impact on the Kansas economy and many lab
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1/ 👋 Excited to share what we’ve been building at https://t.co/GOQJ7LjQ2t + we are going to tweetstorm our progress every week!
Week 1 highlights: getting shortlisted for YC W2019🤞, acquiring a premium domain💰, meeting Substack's @hamishmckenzie and Stripe CEO @patrickc 🤩
2/ So what is Brew?
brew / bru : / to make (beer, coffee etc.) / verb: begin to develop 🌱
A place for you to enjoy premium content while supporting your favorite creators. Sort of like a ‘Consumer-facing Patreon’ cc @jackconte
(we’re still working on the pitch)
3/ So, why be so transparent? Two words: launch strategy.
jk 😅 a) I loooove doing something consistently for a long period of time b) limited downside and infinite upside (feedback, accountability, reach).
cc @altimor, @pmarca
4/ https://t.co/GOQJ7LjQ2t domain 🍻
It started with a cold email. Guess what? He was using BuyMeACoffee on his blog, and was excited to hear about what we're building next. Within 2w, we signed the deal at @Escrowcom's SF office. You’re a pleasure to work with @MichaelCyger!
5/ @ycombinator's invite for the in-person interview arrived that evening. Quite a day!
Thanks @patio11 for the thoughtful feedback on our YC application, and @gabhubert for your directions on positioning the product — set the tone for our pitch!
Week 1 highlights: getting shortlisted for YC W2019🤞, acquiring a premium domain💰, meeting Substack's @hamishmckenzie and Stripe CEO @patrickc 🤩
2/ So what is Brew?
brew / bru : / to make (beer, coffee etc.) / verb: begin to develop 🌱
A place for you to enjoy premium content while supporting your favorite creators. Sort of like a ‘Consumer-facing Patreon’ cc @jackconte
(we’re still working on the pitch)
3/ So, why be so transparent? Two words: launch strategy.
jk 😅 a) I loooove doing something consistently for a long period of time b) limited downside and infinite upside (feedback, accountability, reach).
cc @altimor, @pmarca
4/ https://t.co/GOQJ7LjQ2t domain 🍻
It started with a cold email. Guess what? He was using BuyMeACoffee on his blog, and was excited to hear about what we're building next. Within 2w, we signed the deal at @Escrowcom's SF office. You’re a pleasure to work with @MichaelCyger!
5/ @ycombinator's invite for the in-person interview arrived that evening. Quite a day!
Thanks @patio11 for the thoughtful feedback on our YC application, and @gabhubert for your directions on positioning the product — set the tone for our pitch!
THIS.
Russia hasn't been a willing partner in this treaty for almost 3 decades. We should have ended the pretense long ago.
Naturally, Rand Paul is telling anyone who will listen to him that Trump is making a HUGE MISTAKE here.
Rand is just like his dad, Ron. 100% isolationist.
They've never grasped that 100% isolationist is not 'America First' when you examine it. It really means 'America Alone'.
The consistent grousing of pursuing military alliances with allies - like Trump is doing now with Saudi Arabia.
So of course Rand has also spent the last 2 days loudly calling for Trump to kill the arms deal with Saudi Arabia and end our alliance with them.
What Obama was engineering with his foreign policy was de facto isolationism: pull all the troops out of the ME, abandon the region to Iranian control as a client state of Russia.
Obama wasn't building an alliance with Iran; he was facilitating abandoning the ME to Iran.
Obama wouldn't even leave behind a token security force, so of course what happened was the rise of ISIS. He also pumped billions of dollars into the Iranian coffers, which the Mullah's used to fund destabilizing activity [wars/terrorism] & criminal enterprises all over the globe
Russia hasn't been a willing partner in this treaty for almost 3 decades. We should have ended the pretense long ago.
Naturally, Rand Paul is telling anyone who will listen to him that Trump is making a HUGE MISTAKE here.
Arms control agreements are good when you have willing partners. Lightens the load on our military.
— John Noonan (@noonanjo) October 20, 2018
Russia hasnt been a willing partner in years. There will be gnashing of teeth from people who do arms control advocacy full time, but this is right movehttps://t.co/WmQE43ERCB
Rand is just like his dad, Ron. 100% isolationist.
They've never grasped that 100% isolationist is not 'America First' when you examine it. It really means 'America Alone'.
The consistent grousing of pursuing military alliances with allies - like Trump is doing now with Saudi Arabia.
So of course Rand has also spent the last 2 days loudly calling for Trump to kill the arms deal with Saudi Arabia and end our alliance with them.
What Obama was engineering with his foreign policy was de facto isolationism: pull all the troops out of the ME, abandon the region to Iranian control as a client state of Russia.
Obama wasn't building an alliance with Iran; he was facilitating abandoning the ME to Iran.
Obama wouldn't even leave behind a token security force, so of course what happened was the rise of ISIS. He also pumped billions of dollars into the Iranian coffers, which the Mullah's used to fund destabilizing activity [wars/terrorism] & criminal enterprises all over the globe
