Keep dwelling on this:
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.
https://t.co/EwwJOSa8RK

https://t.co/BsY8BAIzDa

https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs

Keep on Puffing, Ignore the Guys in White Coats!
https://t.co/EWmVWI5jYr
Anyway, this is where Cheng et al (2020) got the neurotoxin motifs from
Superantigenic character of insert unique to SARS-CoV-2
https://t.co/EwwJOSa8RK

Bracci L., Ballas S. K., Spreafico A., Neri P., Molecular mimicry between the rabies virus glycoprotein and human immunodeficiency virus-1 GP120: cross-reacting antibodies induced by rabies vaccination.
https://t.co/UZbe658OGR

(apart from being good news for smokers...)
Maybe someone else can unravel the implications?
@Parsifaler @w_mccairn @Rossana38510044

"For binding experiments, cells were harvested mechanically with a rubber policeman & centrifuged"
Binding of HIV-1 gp120 to the nicotinic receptor (1992)
https://t.co/6veoEkVbVp
Referenced in Bracci et al (1997)
https://t.co/UZbe658OGR

Sequence homology between HIV gp120, rabies virus glycoprotein, and snake venom neurotoxins - Is the nicotinic acetylcholine receptor an HIV receptor?
https://t.co/XGVUNyORP3

(Sönnerborg & Johansson, 1993)
The neurotoxin-like sequence of human immunodeficiency virus GP120: A comparison of sequence data from patients with and without neurological symptoms
https://t.co/s8DIeMNMmD

1. Molecular mimicry of HIV gp120 (2005) https://t.co/KzfEpbjbvk
2. McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
https://t.co/iG8NSrGrlI
3. Snake envenomation https://t.co/uJy1bi7NEU
4. Modified venom and venom components as anti-retroviral agents (PATENT)
https://t.co/VRH0gJlbqM
5. Recombinant Newcastle disease virus (NDV) strain rL-RVG expressing the rabies virus glycoprotein
https://t.co/31SNcPXNYJ
6. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein
https://t.co/Ce9cAEOCQH
Role of HIV gp120 in crossing blood brain barrier
Interactions of SARS-CoV-2 with the Blood–Brain Barrier
https://t.co/oZWRqn5cGr
HIV-1 protein gp120 crosses the blood-brain barrier role of adsorptive endocytosis
https://t.co/9pdkqSt0xz

Final Thesis Jack Sim
In-vitro Characterisation of Targeting Ligands for
Enhanced Delivery Across the Blood-Brain Barrier
https://t.co/GAURhTA376
https://t.co/XQ71CTh93k

Simulations support the interaction of the SARS-CoV-2 spike protein with nicotinic acetylcholine receptors and suggest subtype specificity
https://t.co/umDn3S549X

Simulations support the interaction of the SARS-CoV-2 spike protein with nicotinic acetylcholine receptors
https://t.co/1Qg0tKg3YI
Media Review:
https://t.co/2yyYJw3zVv
2. SARS-CoV-2 spike may act as a superantigen to trigger the development of MIS-C as well as cytokine storm in adult COVID-19 patients
https://t.co/sXQyd7kMx5

COVID-19–associated multisystem inflammatory syndrome in children (MIS-C): A novel disease that mimics toxic shock syndrome—the superantigen hypothesis
https://t.co/NxmPM719VB

https://t.co/b8PssOG6my
SEB confirmed again

1. What is Happening with Smokers and COVID-19?
https://t.co/wXGYU9EjAp
2. Cytokine Release Syndrome (CRS) and Nicotine in COVID-19 Patients
https://t.co/3oA9sWOTaU
and
https://t.co/4yGz47igy2
3. Involvement of nicotine receptors in COVID-19
https://t.co/0GtJwhu9tU

Who put the Benzedrine in Mrs Murphy's Ovaltine?
Who put SAg & SEB in SARS COV-2's Spike Protein?
Note:
A monoclonal antibody against staphylococcal enterotoxin B superantigen inhibits SARS-CoV-2 entry
https://t.co/x7iAVt4Zvm

But a solution is proposed...
A monoclonal antibody against staphylococcal enterotoxin B superantigen inhibits SARS-CoV-2 entry in vitro
https://t.co/x7iAVt4Zvm
and
https://t.co/NgZ7aNd2KS

Molecular mimicry between rabies virus glycoprotein & HIV-1 GP120
https://t.co/Ahp5mE27uI

A Novel Vaccine Employing Non-Replicating Rabies Virus Expressing Chimeric SARS-CoV-2 Spike Protein Domains: Functional Inhibition of Viral/Nicotinic Acetylcholine Receptor Complexes
https://t.co/Zp6gHLPE1x

Persistent SARS-2 infections contribute to long COVID-19
https://t.co/R5UI7pOynJ
For the Curious:
Dampened Bat Immune System Interferon Response?

Why COVID-19 Transmission Is More Efficient and Aggressive Than Viral Transmission in Previous Coronavirus Epidemics?
https://t.co/yNTOP9Lf37
Good Luck with finding the answers!
https://t.co/9iQQBiUjaS
References 145, 146 & 147 for the curious:
https://t.co/yNTOP9Lf37
Host resistance influences patterns of experimental viral adaptation
https://t.co/gnLm32z0G6

More from Billy Bostickson 🏴👁&👁 🆓
1. WIV Research groups (archived pages) for Reference
dsRNA viruses molecular biology
https://t.co/lwrQoo6ygG
2. Emerging Viruses Group
https://t.co/ND56gVGOAn
3. Structural Biology of Viral Genome Replication
https://t.co/wUqc0YIP3X
4. Molecular Biology of hepatitis Viruses & Gene Therapy
https://t.co/snBbxkhNsC
5. Insect Virus Genetic Engineering Lab
https://t.co/S68mVOA2Ob
dsRNA viruses molecular biology
https://t.co/lwrQoo6ygG

2. Emerging Viruses Group
https://t.co/ND56gVGOAn

3. Structural Biology of Viral Genome Replication
https://t.co/wUqc0YIP3X

4. Molecular Biology of hepatitis Viruses & Gene Therapy
https://t.co/snBbxkhNsC

5. Insect Virus Genetic Engineering Lab
https://t.co/S68mVOA2Ob

1. Messy Thread exploring connections between
@DARPA @doddtra PREDICT & @EcoHealthNYC
What happened to FLIRT?
https://t.co/wgttFlgcP1
EcoHealth Alliance analyzed all airports in the US by assessing how often passengers trickle in from areas with confirmed cases of Zika virus.
2. “I believe this is something that people should know,” said Andrew Huff, vice-president of group, who oversees efforts to forecast the spread of infectious diseases. “If you know where an infectious disease is, you can take precautions.”
Andrew Huff used to run their database
3. Andrew Huff
https://t.co/yqP8NbiN5X
Data papers:
https://t.co/mCdcAMTHNW
FLIRT (Zika)
https://t.co/MyUM3mt6c3
MANTLE https://t.co/dBsFAixRXv
MANTLE
4. GRITS and EcoHealth Alliance and DTRA
Global Disease Warning Software - May 28, 2015
EcoHealth Alliance and its Partners Create Biosurveillance Application with $2.2 million grant from the DTRA to continue the development of disease warning software
Global Rapid Identification Tool System (GRITS) software developed with ProMED, International Society for Infectious Disease, & Kitware
Biosurveillance app that enables infectious disease analysts to monitor textual data sources for infectious disease threats in near real time.
@DARPA @doddtra PREDICT & @EcoHealthNYC
What happened to FLIRT?
https://t.co/wgttFlgcP1
EcoHealth Alliance analyzed all airports in the US by assessing how often passengers trickle in from areas with confirmed cases of Zika virus.
2. “I believe this is something that people should know,” said Andrew Huff, vice-president of group, who oversees efforts to forecast the spread of infectious diseases. “If you know where an infectious disease is, you can take precautions.”
Andrew Huff used to run their database

3. Andrew Huff
https://t.co/yqP8NbiN5X
Data papers:
https://t.co/mCdcAMTHNW
FLIRT (Zika)
https://t.co/MyUM3mt6c3
MANTLE https://t.co/dBsFAixRXv
MANTLE
4. GRITS and EcoHealth Alliance and DTRA
Global Disease Warning Software - May 28, 2015
EcoHealth Alliance and its Partners Create Biosurveillance Application with $2.2 million grant from the DTRA to continue the development of disease warning software
Global Rapid Identification Tool System (GRITS) software developed with ProMED, International Society for Infectious Disease, & Kitware
Biosurveillance app that enables infectious disease analysts to monitor textual data sources for infectious disease threats in near real time.
Zhengli Shi and Deyin Guo in 2015
Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Increase or Decrease Replication and Pathogenesis
https://t.co/YMlsqFOvZp
Deyin Guo 2016:
Molecular mechanisms of coronavirus RNA capping and methylation
https://t.co/xdEU5goLB6
Deyin Guo 2012
Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2′-O-methyltransferase activity of nsp10/nsp16 complex
https://t.co/x30jPYFDTa
Deyin Guo 2013
Structure-function Analysis of SARS Coronavirus RNA Cap Guanine-N7 Methyltransferase.
https://t.co/NG57b85hcs
Deyin Guo 2016
Identification and Characterization of a Ribose 2’-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales
https://t.co/zZ5V7erseD
Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Increase or Decrease Replication and Pathogenesis
https://t.co/YMlsqFOvZp

Deyin Guo 2016:
Molecular mechanisms of coronavirus RNA capping and methylation
https://t.co/xdEU5goLB6

Deyin Guo 2012
Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2′-O-methyltransferase activity of nsp10/nsp16 complex
https://t.co/x30jPYFDTa

Deyin Guo 2013
Structure-function Analysis of SARS Coronavirus RNA Cap Guanine-N7 Methyltransferase.
https://t.co/NG57b85hcs

Deyin Guo 2016
Identification and Characterization of a Ribose 2’-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales
https://t.co/zZ5V7erseD

November 3 - 9, 2019 -Wuhan Institute of Virology
International Training Course on Biosafety Laboratory Management and Technology
1. Daniel Feakes, head of UN Biological Weapons Convention implementation support agency
2. René Courcol, quality control system expert, France's BSL
List of Lecturers with short bios at
November 3 - 9, WIV Training Course: https://t.co/Bsd9yO40JP
YUAN Zhiming Professor, Wuhan Institute of Virology, CAS (Deputy Director)
SONG Donglin, Professor, Wuhan Institute of Virology, CAS ( Deputy Director of Wuhan BSL-4 Laboratory.)
Dr. TONG Xiao Senior Engineer at WIV (Molecular Virology & (HIV) infection, mechanical & vaccine immunology) On-site Manager & Chief engineer for WIV BSL-4 Lab construction, maintenance & biosafety equipment R&D.
Zhengli Shi, Director of WIV Center for Emerging Infectious Diseases, now in charge of scientific activity in BSL3 & BSL4. Viral pathogen discovery through sequencing techniques, wildlife-borne viral pathogens, particularly bat-borne viruses since 2004.
DENG Fei, Principle Investigator in Research Group on Viruses Resources & Biotechnology in WIV, also Director of Microorganisms & Viruses Resource Center.
Graduate of ECUST, went to Great Lakes Forestry Center, Canada in 2001 & Wageningen University, NL to study baculovirus.
International Training Course on Biosafety Laboratory Management and Technology
1. Daniel Feakes, head of UN Biological Weapons Convention implementation support agency
2. René Courcol, quality control system expert, France's BSL

List of Lecturers with short bios at
November 3 - 9, WIV Training Course: https://t.co/Bsd9yO40JP
YUAN Zhiming Professor, Wuhan Institute of Virology, CAS (Deputy Director)
SONG Donglin, Professor, Wuhan Institute of Virology, CAS ( Deputy Director of Wuhan BSL-4 Laboratory.)
Dr. TONG Xiao Senior Engineer at WIV (Molecular Virology & (HIV) infection, mechanical & vaccine immunology) On-site Manager & Chief engineer for WIV BSL-4 Lab construction, maintenance & biosafety equipment R&D.
Zhengli Shi, Director of WIV Center for Emerging Infectious Diseases, now in charge of scientific activity in BSL3 & BSL4. Viral pathogen discovery through sequencing techniques, wildlife-borne viral pathogens, particularly bat-borne viruses since 2004.
DENG Fei, Principle Investigator in Research Group on Viruses Resources & Biotechnology in WIV, also Director of Microorganisms & Viruses Resource Center.
Graduate of ECUST, went to Great Lakes Forestry Center, Canada in 2001 & Wageningen University, NL to study baculovirus.
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