BC ALL
Saved by @BillyBostickson

Keep dwelling on this:
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.

 The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains
(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK
In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa
Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs
Y674QTQTNSPRRAR685
Reference 21:
A nicotinic hypothesis for Covid-19
https://t.co/EWmVWI5jYr
Above all, fantastic news for chain smokers! (if true)
Keep on Puffing, Ignore the Guys in White Coats!
https://t.co/EWmVWI5jYr
Anyway, this is where Cheng et al (2020) got the neurotoxin motifs from
Superantigenic character of insert unique to SARS-CoV-2
https://t.co/EwwJOSa8RK
Reference 22 (from preprint) reveals more...
Bracci L., Ballas S. K., Spreafico A., Neri P., Molecular mimicry between the rabies virus glycoprotein and human immunodeficiency virus-1 GP120: cross-reacting antibodies induced by rabies vaccination.
https://t.co/UZbe658OGR
Something important here but I can't connect the dots
(apart from being good news for smokers...)
Maybe someone else can unravel the implications?
@Parsifaler @w_mccairn @Rossana38510044
The Rubber Policeman..
"For binding experiments, cells were harvested mechanically with a rubber policeman & centrifuged"
Binding of HIV-1 gp120 to the nicotinic receptor (1992)
https://t.co/6veoEkVbVp
Referenced in Bracci et al (1997)
https://t.co/UZbe658OGR
earliest reference to this would be in 1990 by same authors (Bracci et al)
Sequence homology between HIV gp120, rabies virus glycoprotein, and snake venom neurotoxins - Is the nicotinic acetylcholine receptor an HIV receptor?
https://t.co/XGVUNyORP3
HIV GP120 /Snake Venom/Rabies GP Neurotoxin loop confirmed
(Sönnerborg & Johansson, 1993)
The neurotoxin-like sequence of human immunodeficiency virus GP120: A comparison of sequence data from patients with and without neurological symptoms
https://t.co/s8DIeMNMmD
Some loose references I don't have time to explore now:
1. Molecular mimicry of HIV gp120 (2005) https://t.co/KzfEpbjbvk
2. McCoy cell line as a possible model containing CD4+ receptors for the study of HIV-1 replication
https://t.co/iG8NSrGrlI
Some loose references I don't have time to explore now:
3. Snake envenomation https://t.co/uJy1bi7NEU
4. Modified venom and venom components as anti-retroviral agents (PATENT)
https://t.co/VRH0gJlbqM
Loose references I don't have time to explore now:
5. Recombinant Newcastle disease virus (NDV) strain rL-RVG expressing the rabies virus glycoprotein
https://t.co/31SNcPXNYJ
6. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein
https://t.co/Ce9cAEOCQH
One way that SARS-COV-2 gets in your brain
Role of HIV gp120 in crossing blood brain barrier
Interactions of SARS-CoV-2 with the Blood–Brain Barrier
https://t.co/oZWRqn5cGr
HIV-1 protein gp120 crosses the blood-brain barrier role of adsorptive endocytosis
https://t.co/9pdkqSt0xz
Jack Sim's Final Thesis before something happened to him down at the Cardiff Docks...
Final Thesis Jack Sim
In-vitro Characterisation of Targeting Ligands for
Enhanced Delivery Across the Blood-Brain Barrier
https://t.co/GAURhTA376
A potential interaction between the SARS-CoV-2 spike protein and nicotinic acetylcholine receptors
https://t.co/XQ71CTh93k
Binding Confirmed Again
Simulations support the interaction of the SARS-CoV-2 spike protein with nicotinic acetylcholine receptors and suggest subtype specificity
https://t.co/umDn3S549X
Above paper also here:
Simulations support the interaction of the SARS-CoV-2 spike protein with nicotinic acetylcholine receptors
https://t.co/1Qg0tKg3YI
Media Review:
https://t.co/2yyYJw3zVv
1. SAg-like motif on SARS-CoV-2 spike distinguished by its high sequence- & structural-similarity to a segment of SEB.
2. SARS-CoV-2 spike may act as a superantigen to trigger the development of MIS-C as well as cytokine storm in adult COVID-19 patients
https://t.co/sXQyd7kMx5
SAg and SEB gonna get you!
COVID-19–associated multisystem inflammatory syndrome in children (MIS-C): A novel disease that mimics toxic shock syndrome—the superantigen hypothesis
https://t.co/NxmPM719VB
Can COVID-19 pandemic boost the epidemic of neurodegenerative diseases?
https://t.co/b8PssOG6my
SEB confirmed again
Smoking Break
1. What is Happening with Smokers and COVID-19?
https://t.co/wXGYU9EjAp
2. Cytokine Release Syndrome (CRS) and Nicotine in COVID-19 Patients
https://t.co/3oA9sWOTaU
and
https://t.co/4yGz47igy2
3. Involvement of nicotine receptors in COVID-19
https://t.co/0GtJwhu9tU
Back to the SEB Mystery - @w_mccairn @Parsifaler
Who put the Benzedrine in Mrs Murphy's Ovaltine?
Who put SAg & SEB in SARS COV-2's Spike Protein?
Note:
A monoclonal antibody against staphylococcal enterotoxin B superantigen inhibits SARS-CoV-2 entry
https://t.co/x7iAVt4Zvm
Nasty, very Nasty!
But a solution is proposed...
A monoclonal antibody against staphylococcal enterotoxin B superantigen inhibits SARS-CoV-2 entry in vitro
https://t.co/x7iAVt4Zvm
and
https://t.co/NgZ7aNd2KS
A nice summary of the issue of mimicry between HIV-1 gp120, Rabies Glycoprotein, Snake Neurotoxin, as well as the role of Nicotinic Receptors, hence smoking as prophylaxis:

Molecular mimicry between rabies virus glycoprotein & HIV-1 GP120
https://t.co/Ahp5mE27uI
This concept has even evolved into a vaccine
A Novel Vaccine Employing Non-Replicating Rabies Virus Expressing Chimeric SARS-CoV-2 Spike Protein Domains: Functional Inhibition of Viral/Nicotinic Acetylcholine Receptor Complexes
https://t.co/Zp6gHLPE1x
Superantigens and Long Covid?
Persistent SARS-2 infections contribute to long COVID-19
https://t.co/R5UI7pOynJ
For the Curious:
Dampened Bat Immune System Interferon Response?
Let's finish with a question:
Why COVID-19 Transmission Is More Efficient and Aggressive Than Viral Transmission in Previous Coronavirus Epidemics?
https://t.co/yNTOP9Lf37
Good Luck with finding the answers!
Serial passage is likely a factor in this issue, as @Harvard2H pointed out:
https://t.co/9iQQBiUjaS
References 145, 146 & 147 for the curious:
https://t.co/yNTOP9Lf37
Host resistance influences patterns of experimental viral adaptation
https://t.co/gnLm32z0G6

More from Billy Bostickson 🏴👁&👁 🆓

Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
Emergent Bio-Kramer-BARDA-HHS-Kadlec-Fauci
U.S. Bet Big on Covid Vaccine Manufacturer Even as Problems Mounted https://t.co/o2lbFoXF5U
“Viral cross-contamination confirmed in the control cells for JANSSEN GMP Lot #8.”


an examination by The @nytimes of manufacturing practices at the Baltimore facility found serious problems, including a corporate culture that often ignored or deflected missteps and a government sponsor, BARDA, that acted more as a partner than a policeman."


https://t.co/3TszTYVhry


https://t.co/ng1oV1xrKI


https://t.co/TNQMo8LZ83
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
PCR Drosten Fail Compilation
1. Ten Fatal Errors: Scientists Attack Paper That Established Global PCR Driven Lockdown
https://t.co/25p88F2xu6
2. Review report Corman-Drosten et al.
https://t.co/8rlT4MWoyI
3. Consequences of false

PCR Drosten Fail Compilation
4. Files for download
https://t.co/SqDrZjB9em
5. Addendum
https://t.co/IygCWvnAsP
6. False positives in reverse transcription PCR testing for SARS-CoV-2
https://t.co/HxpSZkzZ7Q
7. Discussion on Retraction

PCR Drosten Fail Compilation
8. 124 Tweet Thread by Bobby Rajesh Malhotra
@Bobby_Network
https://t.co/cdot5yIeuf
9. Another shorter thread by @Bobby_Network
https://t.co/kqFW9GsSeD
10. Thread by Abir Ballan

PCR Drosten Fail Compilation
11. Mini Thread by @Kevin_McKernan
https://t.co/pntydnd80L
12. Another longer thread by @Kevin_McKernan
https://t.co/5hBYIlXFnQ
13.
ALL
Advertisement
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1/ x Military Games - Wuhan - Coronavirus Reports
@Rossana38510044 @TheSeeker268 @PaulGodolphin
Can anyone follow up with the reporters on the following news stories about military athletes claiming to have coronavirus infection after the Wuhan Military Games in October 2019?

2/ x

3/x

4/ x Italy
“When we talked to a military medic recently, he told us: ‘I think you already had it, because most of the delegation who went to Wuhan got

5/ x India:
"Sunday Guardian wrote to MOD, India, seeking details like whether personnel who returned from Wuhan were checked for Covid-19 symptoms following outbreak of Covid-19. However, no response was received from the
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
@HL3133 @BrendanEich @USRightToKnow ECohealth - DTRA

1. by @samhusseini https://t.co/Yg3HSTnUat

2. https://t.co/EKR0WMFpgn

Very Complete by @AlexisBadenMaye and @OrganicConsumer

3. https://t.co/pAF8IjPqjZ


@BrendanEich @USRightToKnow @samhusseini @AlexisBadenMaye @OrganicConsumer More detailed documents
4. EcoHealth Alliance – Global Rapid Identification Tool Set (GRITS) $4,479,678.00 (Apr 2015) https://t.co/NLyYY8P3BE
5. https://t.co/v7CRmkZqS7
6.
https://t.co/MlePfQbnWF
7. EcoHealth Funding as of December 2020

@BrendanEich @USRightToKnow @samhusseini @AlexisBadenMaye @OrganicConsumer 8. EcoHealth Funding as of 01/10/202
https://t.co/kwD5GFv2My
9. Comparative Spillover Dynamics of Avian Influenza in Endemic Countries
https://t.co/WK3ZhwFhUY
10. https://t.co/KMTeZgQP0F
11. https://t.co/lScigjAWXy
12.

@BrendanEich @USRightToKnow @samhusseini @AlexisBadenMaye @OrganicConsumer 13 https://t.co/b84I413mey
(Bat research with Epstein, Olival & Martha Stokes DTRA)
Stokes is currently working in SE Asia
Evidence for SARS-CoV-2 related coronaviruses circulating in bats & pangolins in SE Asia
https://t.co/40E5gnowoO
Soem Projects
https://t.co/0quX6R44T5


@BrendanEich @USRightToKnow @samhusseini @AlexisBadenMaye @OrganicConsumer 14. Some useful threads
1. Pasteurised Cambodian Bat Fudge https://t.co/2HZREXlI83
2. Project 1742 (EcoHealth/DTRA)
Risks of bat-borne zoonotic diseases in Western
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
Tip from the Monkey
Pangolins, September 2019 and PLA are the key to this mystery
Stay Tuned!


1. Yang


2. A jacobin capuchin dangling a flagellin pangolin on a javelin while playing a mandolin and strangling a mannequin on a paladin's palanquin, said Saladin
More to come tomorrow!


3. Yigang Tong
https://t.co/CYtqYorhzH
Archived: https://t.co/ncz5ruwE2W


4. YT Interview
Some bats & pangolins carry viruses related with SARS-CoV-2, found in SE Asia and in Yunnan, & the pangolins carrying SARS-CoV-2 related viruses were smuggled from SE Asia, so there is a possibility that SARS-CoV-2 were coming from
ALL

More from All

Liz (Welsh) Beechinor \U0001f605
Liz (Welsh) Beechinor ...
@LizTweetsTech
My dad led an organization of over 300,000 employees.

When I took on my biggest marketing & events team to date (35 employees) I asked him how the *heck* he did it. Here's what he said and what helped me every day. 🧵

Remember you're leading all the employees under you, but you're not *managing* them all. You're managing your direct reports, which should be 5-7 individuals max. Focus on them.

When you interact with the employees you don't manage directly, get to know them as people first, that'll be the most valuable information in leading them. Remembering everything you learn about them is hard, but doing so will make you a superhero.

If you're not going to empower the managers under you, why do you have them? If you see room for improvement, let the manager know and let them make the change on their teams and come to you with questions/concerns. This will save you SO much time.

If you're the smartest person in any room - you're doing it wrong. As a leader, your job is bringing together the best people to get the job done - your skillset is identifying those people, not being one of them.
ALL , WORK , A CATAGORY
Liz (Welsh) Beechinor \U0001f605
Liz (Welsh) Beechinor ...
@LizTweetsTech
My dad led an organization of over 300,000 employees.

When I took on my biggest marketing & events team to date (35 employees) I asked him how the *heck* he did it. Here's what he said and what helped me every day. 🧵

Remember you're leading all the employees under you, but you're not *managing* them all. You're managing your direct reports, which should be 5-7 individuals max. Focus on them.

When you interact with the employees you don't manage directly, get to know them as people first, that'll be the most valuable information in leading them. Remembering everything you learn about them is hard, but doing so will make you a superhero.

If you're not going to empower the managers under you, why do you have them? If you see room for improvement, let the manager know and let them make the change on their teams and come to you with questions/concerns. This will save you SO much time.

If you're the smartest person in any room - you're doing it wrong. As a leader, your job is bringing together the best people to get the job done - your skillset is identifying those people, not being one of them.
ALL , WORK , A CATAGORY
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Liz (Welsh) Beechinor \U0001f605
Liz (Welsh) Beechinor ...
@LizTweetsTech
My dad led an organization of over 300,000 employees.

When I took on my biggest marketing & events team to date (35 employees) I asked him how the *heck* he did it. Here's what he said and what helped me every day. 🧵

Remember you're leading all the employees under you, but you're not *managing* them all. You're managing your direct reports, which should be 5-7 individuals max. Focus on them.

When you interact with the employees you don't manage directly, get to know them as people first, that'll be the most valuable information in leading them. Remembering everything you learn about them is hard, but doing so will make you a superhero.

If you're not going to empower the managers under you, why do you have them? If you see room for improvement, let the manager know and let them make the change on their teams and come to you with questions/concerns. This will save you SO much time.

If you're the smartest person in any room - you're doing it wrong. As a leader, your job is bringing together the best people to get the job done - your skillset is identifying those people, not being one of them.
ALL , WORK , A CATAGORY
\u0936\u094d\u0930\u0940Murali\U0001f1ee\U0001f1f3
श्रीMurali🇮🇳...
@mayamadhava
ஸ்ரீ ராம் ஜெயம் 🙏🚩

ஸ்ரீமத் ராமாயணம் 16 வார்த்தைகளில்.

1. பிறந்தார்
2. வளர்ந்தார்
3. கற்றார்
4. பெற்றார்
5. மணந்தார்
6. சிறந்தார்
7. துறந்தார்
8. நெகிழ்ந்தார்
9. இழந்தார்
10. அலைந்தார்
11. அழித்தார்
12. செழித்தார்
13. துறந்தார்
14. துவண்டார்
15. ஆண்டார்
16. மீண்டார


1. பிறந்தார் - ஸ்ரீராமர் கௌசல்யா தேவிக்கு தசரதரின் ஏக்கத்தைப் போக்கும்படியாக பிறந்தது.

2.வளர்ந்தார் - தசரதர் கௌசல்யை சுமித்திரை கைகேயி ஆகியோர் அன்பிலே வளர்ந்தது.

3. கற்றார் - வஷிஷ்டரிடம் சகல வேதங்கள் ஞானங்கள் கலைகள் முறைகள் யாவும் கற்றது.

4.பெற்றார் - வஷிஷ்டரிடம் கற்ற துனுர்வேதத்தைக் கொண்டு விஸ்வாமித்ரர் யாகம் காத்து விஸ்வாமித்ரரை மகிழ்வித்து பல திவ்ய அஸ்திரங்களை பெற்றது.

5.மணந்தார் - ஜனகபுரியில் சிவனாரின் வில்லை உடைத்து ஜனகர்-சுனயனாவின் ஏக்கத்தை தகர்த்து மண்ணின் மகளாம் சீதையை மணந்தது.

6.சிறந்தார் - அயோத்யாவின் மக்கள் மற்றும் கோசல தேசத்தினர் அனைவர் மனதிலும் தன் உயரிய குணங்களால் இடம் பிடித்து சிறந்து விளங்கியது.

7.துறந்தார் - கைகேயியின் சொல்லேற்று தன்னுடையதாக அறிவிக்கப்பட்ட ராஜ்ஜியத்தை துறந்து வனவாழ்வை ஏற்றது.

8. நெகிழ்ந்தார்
🏹அயோத்தியா நகரின் மக்களின் அன்பைக்கண்டு நெகிழ்ந்தது.
🏹குகனார் அன்பில் நெகிழ்ந்தது.
🏹பரத்வாஜர் அன்பில் நெகிழ்ந்தது.
🏹பரதரின் அப்பழுக்கற்ற உள்ளத்தையும் தன் மீது கொண்டிருந்த பாலனைய அன்பினையும் தன்னலமற்ற குணத்தையும் தியாகத்தையும் விசுவாசத்தையும் கண்டு நெகிழ்ந்தது.
ALL
sriram
sriram
@SriramKannan77
thEvudaiyameenamaay_aamaiyaay_Enamaay
ariyaaykkuRaLaay
moovuruviniraamanaayk
kaNNanaaykkaRkiyaaymudippaankOyil
sEvalodupedaiyannam
sengamalamalarERi_oosalaadi
poovaNaimElthudhaindhezhu
sempodiyaadiviLaiyaadumpunalarangamE.
(Periyazhwar Thirumozhi on Srirangam)


The One who took avataram of Mathsyam, Koormam, Narasimham, Vaamanam, three
Ramams (Parasu Raman, Bala Raman, Kothanda Raman), Krishnan, and Kalki et
al, - taking such avatharams to protect the virtue and saadhus
and,
to destroy the wicked (asurAs) –

Such grandest Lord’s temple is Srirangam divya
Desam-

where the swans (male and females) jointly swim on the red lotus flowers, climb to the bank of the river and drop the powders stuck on their
bodies on the flower bed on land.

EmperuMAn’s avatharams are based on only His krupaa and His sankalpam.

Along with His appearance, the krupa roopi PiraaTTi (the embodiment of daya-
mercy) also takes the respective avatarams to be with the Lord as Nithya anapaauinee
ALL

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Trader knight
Trader knight
@Traderknight007
Pyramiding 2.0

A thread-


What is pyramiding?

It is simply adding to your winners.

Why use pyramiding?

1. It makes your winners bigger than your losers.

• Because you are adding positions when your trades are going your way, you are increasing your gains but your losers will be small comparatively.

2. You lose less during Draw-down time ( it saves you from losing big during whipsaws )

• Many times when the market is in sideways mode, Entering a trade with full risk might not be the best idea.

• So, you test the waters with a small position first, it could be ⅓ or ¼ of your total risk.

• So if the Breakout fails or whipsaws come, then you will only lose a small part of your overall risk.

• Then when position starts to go in your favor you can add to it.

3. It helps you to concentrate on a few trades,

They say that don’t put all your eggs in one basket, but don’t put them in too many either.

• See, Everyone can see charts and find breakouts, pullbacks etc.
ALL , SWING TRADING , PYRAMIDING
Naomi Davis
Naomi Davis
@NaomisLitPix
I want to talk about the editorial support you should expect from an agent who says they work editorially, &what you should not expect, too. (As always these are my opinions; others will think differently; you can take any opinion you encounter or burn it in a trash can.) #Thread

Most agent offer calls come with a discussion about editorial support and expectations on both sides. If an agent says they work editorially, both of you can talk about what you hope this will look like. This may include:

Rounds of overall revision to plot/character/structure/setting, or tightening to a genre guideline, based on an "edit letter."
Line edits/sidebar comments by your agent. I always stress these are suggestions to achieve a goal. How author arrives at that goal is up to to them, but

I should make it clear to you what the goal of the point is: heighten emotional moment, untangle complicated scene, drive a relationship, clarify stakes, etc. I may suggest HOW and you can use my suggestion or come up w/your own solution. As long as the goal is attained.

But I'll always be honest if the changes came up short, too. It's a conversation, a development, open-door for challenge.
I DO put foot down abt blatant "rookie mistakes." Or major arc problems,sensitivity issues. I don't do this to be obstinate;but you should stay flexible too.
WRITING
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Dani\xeblle Stotijn
Daniëlle Stotijn
@DDay35962863
If it doesn,t make sense...
something else is going on.....

#xrp
#xrpcommmunity

Expand your thinking

This thread is updated and can be approached from multiple perspectives.
That is how big Platform 2020 is
#TheGreatAwakeningWorldwide

Connect the dotes
FOR LATER READ , THREAD PLEASE
Biz Stone
Biz Stone
@biz
Originally, tweet length was 160 minus username length. One day, I turned to Jack and pointed out that I got one more character than him. He said, "That's not fair. We should standardize." I agreed. He said, "How about 140?" I said, "Sure." And he updated the code.

Last year, we upgraded tweet length to 280 and guess what? Use of 'please' is up 54% and 'thank you' is up 22%. I love that! Also, people are asking more questions and having more conversations. All this, and the majority of tweets are still under 140. It worked.
TECH
Anarchist \U0001f4a3Federation \u2764\U0001f5a4
Anarchist 💣Federation ...
@AnarchistFed
While watching the GameStop stuff has been mildly hilarious (I mean who doesn't like watching the financial elite squirm?) it's pretty clear that a whole bunch of folk have convinced themselves this is ACTUALLY some kind of revolutionary activity. It isn't. 1/


Sure, it's highlighted yet another way in which the bastards profiteer but that's about it, stick it on the pile with the fifty other pro tips to keep the poor, well poor... It's todays banter and tho a few folks might have egg on their faces, literally nothing will happen. 2/


GameStop's three largest shareholders have earned £1.5bn+ during this. They sit at the top of some 200 institutions which are rolling. Meanwhile the ersatz rebels are now watching the bubble, realising there is profit to be made and having a wibble. 3/


No doubt some TRULY believe they are taking part in some kind of revolution but frankly it's utterly absurd and the vast majority are in it for the lulz and to cash in quick.

I'm sorry to tell you this, but you don't own GameStop.

4/


The reality is, the scale of the economic disparity and corruption is simply vast. Billionaires loosing a few millions means nothing. They buy one less yacht and you continue to live meal to meal.

The "Free Market" changes a few systems and the abuse continues. 5/
TRADING