https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.

@franciscodeasis https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV
@franciscodeasis https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.
@franciscodeasis Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.
@franciscodeasis The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
@franciscodeasis Large-scale transmission and the first report of death in wuhan—which need substantial amount of active cases in order to begin getting reported. This mean that https://t.co/cn4QuN4v6P the highest population density area is most likely to report the first death—this is especially
@franciscodeasis True since an excess pneumonia death record in 01/2020 is already equiliberated, and https://t.co/ZuWTPOW60b it is found that when accounting for population density and aging degree, the JiangAn and the HanYang districts are the two locations with the largest absolute number of
@franciscodeasis Aged population in the entirety of Wuhan. SARS-CoV-2 mortality https://t.co/SNnZjPvxuG is strongly positively associated with age—the seafood market served as a superspreader, and https://t.co/BU4ehyLHMm raise to the major location of cases and the major lineage (B lineage) by
@franciscodeasis Jan 19th. This will of course skew the excess pneumonia deaths to the wrong side of the Yangtze river—after all, the districts that first showed an raise of excess pneumonia deaths were the districts where the majority of Wuhan’s aged population was located.
@franciscodeasis In fact, the highest absolute spacial density of aged population in Wuhan is also in the place near the market—those 3 districts had the highest number of retirement homes in the whole of wuhan—community transmission of SARS-CoV-2 lead to most https://t.co/Rv81pEaEpE
@franciscodeasis excess deaths in retirement homes. This is a far more parsimonious explanation than having the Huanan seafood market, which have no sequence of the ancestral A lineage, as the absolute origin of the SARS-CoV-2 index case.
@franciscodeasis https://t.co/Ib59PY7krz

https://t.co/s70jNIvUb4
As for why “55% of the December 2019 cases were connected to the huanan seafood market”? “Contact history to the huanan seafood market” was literally written into the standard for reporting a case as SARS-CoV-2 infection in Wuhan
@franciscodeasis In the December 2019 standard. “《不明原因的病毒性肺炎入排标准》”

https://t.co/sqsq5x3YRw
In fact, they were still defending their claim of the seafood market as the absolute index case in Wuhan—they only admit “uncertainty” in the case reporting, while still attempt to defend
@franciscodeasis The market claim by using excess pneumonia deaths—while completely ignoring the fact that the districts near the market had the highest amount of retirement homes—the absolute biggest source of SARS-CoV-2 related deaths in the earliest stages of SARS-CoV-2.
@franciscodeasis On the index cases: https://t.co/IZztF2siZq
@franciscodeasis https://t.co/fPp0ihuQhZ
@franciscodeasis https://t.co/4xtzGonSaB
@franciscodeasis https://t.co/YA9BShS9UF
@franciscodeasis https://t.co/dCO7hedYqG
@franciscodeasis https://t.co/LvAvsif4gk
@franciscodeasis https://t.co/id0RNXug8k
@franciscodeasis https://t.co/6yp9kCXKWm
@franciscodeasis
@franciscodeasis
@franciscodeasis
@franciscodeasis If "Most SARS-CoV-2 index case infections will not have resulted in sustained onward transmission" serological and PCR evidence before the outbreak should be present in Wuhan China. However no such evidence were present in either Wuhan or in other places in China.
@franciscodeasis As any local clusters will cause illness, and will be visible to the surveillance system, leading to sample collection and positive results. however, all 92 cases that were screened to be compatible with SARS-CoV-2 infection turned out to be negative.
@franciscodeasis https://t.co/llMGT0JLx5 " Poliovirus is, however, strictly a human pathogen, and does not naturally infect any other species (although chimpanzees and Old World monkeys can be experimentally infected).[46]" https://t.co/ZxJaD9sWn8
@franciscodeasis Unfortunately, They got the entire basic of Polio wrong. Polio is a pathogen that is certified to exclusively infect humans.https://t.co/BeDXMpLYUa
@franciscodeasis "HCV has a narrow host range infecting only humans and chimpanzees." https://t.co/BeDXMpLYUa The same applies to Hepatitis C virus, which is also a certified human pathogen that exclusively infect humans.
@franciscodeasis "Hepatitis C virus displays a narrow host species tropism, naturally infecting only humans, although chimpanzees are also susceptible to experimental infection. To date, there is no evidence for an animal reservoir of viruses closely related to hepatitis C virus which may have
@franciscodeasis crossed the species barrier to cause disease in humans and resulted in the current pandemic. In fact, due to this restricted host range, a robust immunocompetent small animal model is still lacking,
@franciscodeasis hampering mechanistic analysis of virus pathogenesis, immune control and prophylactic vaccine development."https://t.co/tKFaqESIRn Unfortunately, TWO exclusively human and decidedly non-zoonotic pathogens were defaulted as being of "zoonotic origin" in the holmes "article".
@franciscodeasis "Most of the genera in this family infect primarily humans and Old World anthropoids (Fig. 10), with nonprimate strains appearing to derive from their human counterparts (Rodrigo and Dopazo, 1995; Gromeier et al., 1999). " https://t.co/eYwQmdnzKP Polio and other Picornaviruses
@franciscodeasis infecting primates ORIGINATED in and COEVOLVED with humans--there will never be any animal origin for these.
@franciscodeasis https://t.co/AYYICizVd4
https://t.co/wDDy1s6Tmk
On SARS-CoV and MERS-CoV: Highly prevalent in Civets, Raccoon dogs in market, as well as in camel herds.
@franciscodeasis https://t.co/UJQrANyLkE
https://t.co/WeI8UOx9R8
On seroprevalence of SARS-CoV and MERS-CoV.
@franciscodeasis https://t.co/mb8Rfwnhg6
@franciscodeasis https://t.co/Kp6qfNl5P6
https://t.co/18qKGTXn0y
https://t.co/Xx7GjNOiEc
@franciscodeasis https://t.co/Egj9ZdAp1i for everything else.
@franciscodeasis https://t.co/dEbk3F3WjC
On RaTg13.
@franciscodeasis https://t.co/8kHw65luEH Why a mutation in humans generated a RdRp that is more permissive to RDV binding than the original? https://t.co/R0YmWMsOx3
The RdRp had an anomalously high resistance to Remdesivir—best explained by passaging in the presence of RDV/RTP.
@franciscodeasis https://t.co/jkdByR7Ia8
Mojiang miners. This is why the oral serological claims made by the WIV can not be trusted in any way. No Data for the Shi addendum, or any of the claimed seronegativity of the WIV workers.
@franciscodeasis https://t.co/0vmXzMDU0B
SARS-CoV-2 LAI covered up for a year and a half.
@franciscodeasis Also important is that the cases that were found in the Huanan market was sampled about the same time as the lineage A genomes in Wuhan, and that at that time (December-January 2020, before 01/19/2020 where PCR testing kits becomes available), contact tracing to the wet market
@franciscodeasis (worked, entered, or have contacted others that have visited) was required for the diagnosis of SARS-CoV-2 infection--and only patients that had geological proximity to the market were sampled for sequencing before 19 January 2020. (as contact traced history to the Huanan market
@franciscodeasis was required for diagnosis of the "unknown pneumonia cases" in wuhan at that time, making the sampling of the earliest lineage A genomes necessarily biased to cases that just happened to have geographic proximity to the market. https://t.co/clKNfnJnZ5
@franciscodeasis The first official case had an symptom onset date that is consistent with the incubation period of SARS-CoV-2 Wuhan strain, which is approximately one week after initial exposure, indicating that he was in fact exposed in 08/Dec/2019. https://t.co/D85r2bHHNR
@franciscodeasis Neither the March 2020 interview nor the WHO interview indicate contact history that are closely related to the Huanan seafood market, which "木兰山" was in fact way outside of Wuhan, with visit time that was way in the beginning of November 2019, and zero contact history to the
@franciscodeasis market in any way. there is no "travel to the north of the huanan seafood market shortly before symptoms begin". Notably, at time of the case tallying by RT-PCR have begun, the distribution of cases have already fully matched that of the population density pattern for Wuhan.
@franciscodeasis And the density pattern of elderly people in Wuhan. A serological survey indicate that https://t.co/SIxHjAHFh7 the highest amt of asymptomatic PCR positivity was detected in Wuchang, https://t.co/Ua7FQdqHNv which is consistent with the location being the highest amt of
@franciscodeasis SARS-CoV-2 positivity based on past Influenza prevalence data and COVID-19 positivity (symptomatic)/influenza positivity rates. remember that the only patient samples that hit the sequencers in December 2019 are the samples that were connected (epidemiology, contact tracing)
@franciscodeasis To the Huanan seafood market--in order to be even diagnosed as a case of COVID-19 and have your sample sequenced before 19/01/2020, you must either be in direct contact or be geographically near the Huanan seafood market, within reasonable contact tracing ranges.
@franciscodeasis The Jiangxia case have also denied any contact or travel history to the location where the Huanan seafood market was located at, 15 days before symptom onset. This makes contact with the Huanan market extremely unlikely.
@franciscodeasis In addition, as both of the "earliest A lineage genomes" sampled near, but not actually contacting, the Huanan seafood market (only cases near the market gets to be sequenced. https://t.co/XDgdgLxn70 some hospital sequenced cases from the same period, again right atop the
@franciscodeasis WIBP dormitories, have lineage A genomes as well as other highly diverse genomes. samples were Dec-Jan 2020), were sampled at the same time as the actual cluster case in the Huanan seafood market, the temporal range of these samples overlap in the same course of human SARS-CoV-2
@franciscodeasis infection, Should A lineage be actually present in the market within community transmission time range (market to community), necessary to assign a market origin for these 2 A lineage cases, then there is no reason that the lineage was not present in the market samples--since
@franciscodeasis they would have been sampled within the range of viral persistence in the human body considering their overlapping sampling dates. In stead, despite all the cases within the market being sequenced, even including environmental isolates, none of the genomes were lineage A,
@franciscodeasis which would have been wholly inconsistent with an origin in the Huanan seafood market. as lineage A genomes were also found in the RenMin hospital approximately the same time, it rules out the seafood market as the source of the ancestral A lineage genomes.
@franciscodeasis https://t.co/BZykqhlwu6
Note that there were 2 genomes in Wuhan and 15 out of the 91 genomes from Sichuan before 2021 carrying intermediate between A and B lineage, with sampling outside the market generally returning much greater diversity than inside the market.
@franciscodeasis As the consensus sequence in animals is that of lineage A, T8782/C28144, And since there exist a statistically significant fraction of genomes (1/4 out of all genomes in Sichuan, 2 in Wunan) that matches the exact intermediate lineage from A to B using a rooted tree based on
@franciscodeasis USA/WA-1, Guangdong/HKU/SZ or Shandong/Ly005/2, and that intermediate genomes between A and B lineages are found abundantly within the stool samples of chidren sick with SARS-CoV-2 and respiratory samples of SARS-CoV-2 patients, A is the ancestor of B and there are no need for
@franciscodeasis "two spillovers" to happen at all. all happened in humans. Also there is another issue with the early SARS-CoV-2 case data--as contact tracing to the Huanan seafood market is necessary for the initial (between 20/Dec to 04/Jan) recognition of SARS-CoV-2 cases for sequencing and
@franciscodeasis for the recognition and archival of early cases of SARS-CoV-2(which, as per the deleted SRR dataset that were found right atop the dormitories of the WIBP, a lot of SARS-CoV-2 cases, especially A lineage genomes, w/mutations indicative of substantial diversity in early Jan 2020)
@franciscodeasis , and given the effort of obfuscating and destruction of case records and sequence data outside of the Huanan seafood market for samples that were taken before 04/Jan/2020, and the fact that https://t.co/WiGSze3POM there exist french patients that have no travel history to China
@franciscodeasis , whose throat swab have a positive PCR test in 27/Dec/2019 (requiring at least 1 round of community transmission from a person that recently traveled abroad, pushing the date of first wuhan case onset to at least two weeks before that at ~10 Dec 2019 in case of travel)
@franciscodeasis https://t.co/UyZkEJktqF, as well as some swab PCR positivity in Italy with a date of collection of 05 December, which is before the first Huanan seafood market case, indicate that cases exist in China before the official first case in the Huanan seafood market.
@franciscodeasis https://t.co/QiCSDcKeCD
Then, there is an increase in the amt of S seropositivity in sera collected in France in December 23 2019, which would have required the first widespread transmission of SARS-CoV-2 in france beginning at 09/2019--with substantial amt of infection well
@franciscodeasis past patient zero at that time. https://t.co/FU8RKB1052
Another clue came from italy, which have shown consistent qRT-PCR positivity from wastewater (this is not the november claim with just one sample. this is consistent PR-PCR positivity) since 18/12/2019, which once again
@franciscodeasis would have required that the beginning of transmission (substantial amount of infection, take about two weeks to achieve from patient zero) in both cities to begin well before the claimed 11 December symptom onset of the first Huanan seafood market vendor in 2019.
@franciscodeasis These aggregated data all suggest that SARS-CoV-2 transmission have begun in Europe in the same time as, or even before, the Huanan seafood market cluster, which as international travel for pathogen spread requiring even more time than domestic spread, would be consistent
@franciscodeasis with the lineage A of SARS-CoV-2 emerging before Lineage B and spread to high diversity in Wuchang, and Lineage B emerging in humans in Wuhan (with samples suggesting intermediate lineage in Wuchang) and entering europe at the same time or before the Huanan seafood market.
@franciscodeasis https://t.co/Gq6qPDPaak
Similarily, consistent detection of SARS-CoV-2 have been reported in Brazil between 27/11/2019 and 11/12/2019. Note that A lineage have been found just south of the location being sampled, and is found in South america in general.
@franciscodeasis A lineage also exist in Italy and Spain where the detection of SARS-CoV-2 have been reported that would have pushed the initial outbreak date in Wuhan far before that of the Huanan seafood market, indicating that clade A have in deed been circulating at that time.
@franciscodeasis https://t.co/ButKs5Hoc7
In deed, this would have been consistent with SARS-CoV-2 having a tMRCA in November 17 2019, which is consistent with WIV and WIBP as the origin, with the first outbreak happening in Wuchang, in stead of with the Huanan market as the origin.
@franciscodeasis https://t.co/QKfMWWuljR
In deed, before the more widespread control on the narrative for SARS-CoV-2 origins were established in China, there were reports of contact tracing for first case as early as in 17 November 2019, which is once again earlier than the market, but would have
@franciscodeasis coincided perfectly with the calculated global MRCA of SARS-CoV-2. As samples of SARS-CoV-2 before 18/Jan/2021 was only diagnosed, archived and sequenced if they had geographical or epidemiological link to the huanan seafood market, It shouldn't be surprising that
@franciscodeasis "the earliest patient of SARS-CoV-2 onset was in the huanan seafood market"--that will be the first case that meets the criterion of documentation in that time. https://t.co/0BPmegrJwc
Considering the extreme censorship of early cases in China, and the existence of consistent
@franciscodeasis PCR and serological evidence of SARS-CoV-2 circulation in Italy, France and Brazil at the same time or before the beginning of circulation at the Huanan seafood market, between the end of November 2019 and the beginning of December 2020, The idea that SARS-CoV-2 started in the
@franciscodeasis Huanan seafood market is still inconsistent with actual epidemiological data, and may have willingly precipitated in the CCP's cover-up of earlier cases of SARS-CoV-2 in wuhan.
In deed, much higher diversity exist outside of the market than inside the market for the earliest
@franciscodeasis Wuhan cases, which would be consistent with another source in Wuhan generated the first SARS-CoV-2 human genomes much earlier than the Huanan seafood market, and only one of the branches then entered the seafood market and triggered the monophylectic B lineage outbreak.
@franciscodeasis https://t.co/Y6qK4FoVjR
Certain florida cases also have an onset date at Jan 1 2020.
@franciscodeasis https://t.co/T6QJXgqxvq
There are other possible points before, but they are dubious at best. The first confirmed PCR positivity in Italy and Brazil pushes the arrival date of SARS-CoV-2 at these 2 countries (A, possibly A or B) at 27/11/2019.
https://t.co/nUBbhzXisU
@franciscodeasis This would have been more than a week before the beginning of the first case at the Huanan seafood market--but would have been perfectly fit the 17 November 2019 global TMRCA of SARS-CoV-2 indicated at time before censorship on early contact-tracing and origin-tracing of Wuhan
@franciscodeasis SARS-CoV-2 have took place in China. circa April 2020.
@franciscodeasis another proof that only cases that were market-traced were registered before https://t.co/ZognD2v2jb the beginning of 01 Jan 2020, is that there is a huge gap in the amt of confirmed cases before and after 01/2020, as per the report from CDC. With this in mind, up to
@franciscodeasis 6 times as many cases that weren't associated with the Huanan seafood market were simply erased from the record--they were simply never registered, should they have an epidemiology that is inconsistent with a market-derived origin--if they came before the onset date of the first
@franciscodeasis case in the Huanan seafood market, if they did not contact any person that have visited the market, if they does not live close enough to the market should condition 1 and condition 2 weren't met. You can't just look at data that have been carved out from https://t.co/xLNR9tyJ5J
@franciscodeasis a larger set of (6 times as much!) data based on the connection to the market, and then relying on the coincidence that the HSWM is also where the densest population of people, especially aged people, was located, after that date, to justify your claim of "market origin".
@franciscodeasis https://t.co/yrcb9ki0ke
Very similarly, this happened as well. as many as 4/5 times the active cases were simply removed (not recognized) before the beginning of 2020, simply because they are not epimediologically linked to the Huanan seafood market--you aren't going to find
@franciscodeasis anything that were inconsistent with a plausible "contact traced" epidemiological link with the Huanan seafood market from data that derived before that date--given that epidemiological link to the market was used as the criterion of diagnosis before successful symptom- and PCR-
@franciscodeasis testing were conducted. The CCP feeds you manipulated data, and you will always derive manipulated result from it!
@franciscodeasis

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https://t.co/TVXC4QOjt0
https://t.co/QnMiOrdNhx
https://t.co/ZK2vfwYEFj
in fact, adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures. P681 guards the cardin-weintraub motif against cleavage in

cell lines.

https://t.co/vytn6YVRYQ
https://t.co/fjg6ZXc2KN
the FCS is perfectly stable in anything that isn't VERO E6 classic or 293T-ACE2. anything that had TMPRSS2 and grown in trypsin-free media stably maintains the FCS.

https://t.co/NUrJ8AndTx
in fact, the PRRARS, as opposed to other mutated cleavage sites--even the "perfect" H5CS--confers the greatest infectivity in CaLu-3 cells.

https://t.co/TVXC4QOjt0
in fact, even P681R or S686G changes were less fit in CaLu-3 compared to PRRA virus--the P681R virus show either no difference or is slightly less effective compared to the P681 virus, and the S686G virus
https://t.co/DNtIR17r4S
https://t.co/nDNNw9OaWd
@JackDempsey2_2 @pathogenetics @ggronvall

@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017.
https://t.co/Vh4dKy6rvI there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of

@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things

@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.

@pathogenetics @ggronvall https://t.co/2679qQiA0P
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
@AngloScot2 @WisdomRebel https://t.co/iHKwUoNLJ5

https://t.co/sxm17Eu9hV

https://t.co/FtjifSGItc
PRRA is highly purified in the CaLu-3 cell line when FBS is added to inhibit trypsin. Stu lied about that in his defense on the FCS identity. https://t.co/GzM7uA6vup the Gallaher article contain glaring

@WisdomRebel Mistakes and is completely impossible as HKU9 + RaTG13 only lead to a frameshift inactivated S and not a furin cleaved S.
https://t.co/gmDXK9OluN
The serological test the WIV claimed to be negative in Shi’s addendum is the exact same test they claimed positive in 2012 in the phD

@WisdomRebel thesis, and Stu once again lied about it. https://t.co/Uiy8U9BTYp significant evidence in the form of contaminated SRA datasets suggest that the DEFUSE grant have been funded in China, as the full-length HKU4 is not a part of the 2016-2019 grant, but is found before the start

@WisdomRebel Date of the 2020-2024 grant of June 2020. The WIV also contained several HKU3-like Coronaviruses, particularly in mice in an 2017 GEO experiment, which is consistent with the published HKU3 and batified mice experiment in the DEFUSE document (evidence from an already conducted

@WisdomRebel Experiment). Either DEFUSE or fast-tracked form of the 2020-2024 grant in 2019 would have lead to full-length Sarbecovirus clones being used, leading to SARS-CoV-2 in the lab—they used HKU4 in stead of HKU5 for the MERS spike experiment indicating that backbone sequence distance
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get


A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from

The Taq.
https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals

Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.

The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process

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