https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.
![](https://pbs.twimg.com/media/E9MJvBxUYAQe5ZE.jpg)
![](https://pbs.twimg.com/media/E9MLiVcVgAYtqaC.jpg)
![](https://pbs.twimg.com/media/E9MM9bHUUAMsAWe.jpg)
![](https://pbs.twimg.com/media/E9MPRHYUcAAhisM.jpg)
![](https://pbs.twimg.com/media/E9MShA2VIAMqwR0.jpg)
https://t.co/s70jNIvUb4
As for why “55% of the December 2019 cases were connected to the huanan seafood market”? “Contact history to the huanan seafood market” was literally written into the standard for reporting a case as SARS-CoV-2 infection in Wuhan
https://t.co/sqsq5x3YRw
In fact, they were still defending their claim of the seafood market as the absolute index case in Wuhan—they only admit “uncertainty” in the case reporting, while still attempt to defend
2/ Early MERS/CoV-1 spread showed a 50/50 rate of human versus animal transmission; lots of posterior diversity. With CoV-2, 1001 early Chinese genomes show No... ZERO posterior diversity. Flipping 1001 coins & getting heads every time? That's a one in 10 with 84 zeros chance
— Dr Steven Quay (@quay_dr) December 8, 2020
WHO Report:
— Dr Steven Quay (@quay_dr) June 11, 2021
10,000 archived blood samples in Wuhan & no seroconversion
80,000 animals & no CoV-2
1.9 MM viruses in GISAID & no posterior diversity
All c/w lab origin
People keep saying we don't have evidence for the origin of CoV-2@mattwridley @Ayjchan @AlexBerenson #COVID19
A zoonosis, whether community or laboratory acquired, have 3 elements in common: infected animal(s), viruses, and humans.
— Dr Steven Quay (@quay_dr) May 26, 2021
The differences are natural diversity versus a laboratory singularity.
What do the facts say?#COVID19 pic.twitter.com/K2kEAxGsR0
True but following data must be in the prediction: its not the market, not Hubei bats, not Hubei farmed animals, not pangolins, no Wuhan sero+ background as with SARS-1 & MERS, no post. diversity as with MERS, 1st 50 pts <15 km from world's expert on lab made chimeric CoVs
— Dr Steven Quay (@quay_dr) September 20, 2020
Given that the zoonoses, SARS1 and MERS, had >85% positive tests in markets, the likelihood CoV-2 is a zoonosis like SARS1 or MERS is less than 1 in a 1,000,000.
— Dr Steven Quay (@quay_dr) May 27, 2021
1/ A hallmark of a zoonotic epidemic is community undetected infection pre "Patient Zero." This is detected retrospectively with diagnostic tools looking at archived specimens.
— Dr Steven Quay (@quay_dr) November 1, 2020
Example: Of 7461 archived blood specimens from 2011-2016 in Saudi Arabia, 2.3% were positive for MERS
CoV-1 v. CoV-2:
— Dr Steven Quay (@quay_dr) December 12, 2020
CoV-1: 91 civets & 15 raccoon dogs in wet markets tested: 106/106, 100% positive. https://t.co/BKB4F3p7ev
CoV-2: 0 of 336 specimens from wildlife sold at Hunan market are positive for CoV-2. @gdemaneuf 's nice annotation of the WHO ToR https://t.co/k5r5Hnskbm
2/ 1271 nasal swab & 357 matched blood specimens from patients in Shanghai 1 Dec 2018 to 31 Mar 2020 were examined for CoV-2 by PCR. First positive is 25 Jan 2020.
— Dr Steven Quay (@quay_dr) November 1, 2020
This is not c/w a zoonotic epidemic but is consistent with a lab escape origin.https://t.co/fE9WSpeXS4
https://t.co/wDDy1s6Tmk
On SARS-CoV and MERS-CoV: Highly prevalent in Civets, Raccoon dogs in market, as well as in camel herds.
https://t.co/WeI8UOx9R8
On seroprevalence of SARS-CoV and MERS-CoV.
https://t.co/18qKGTXn0y
https://t.co/Xx7GjNOiEc
(And other clades of SARS-CoV-2)
— Daoyu (@Daoyu15) July 21, 2021
Observation 4: All tested ACE2 favors H,Y or F on position 498 over Q498. The only RBD that is better with Q498 is Rs7327. However, it is not relevant to SARS-CoV-2, and carried a F498 by default, indicating that other constraints (folding) is at pic.twitter.com/e1WN3aFoXc
The RdRp had an anomalously high resistance to Remdesivir—best explained by passaging in the presence of RDV/RTP.
Mojiang miners. This is why the oral serological claims made by the WIV can not be trusted in any way. No Data for the Shi addendum, or any of the claimed seronegativity of the WIV workers.
![](https://pbs.twimg.com/media/FEjH-fKVIAQBqw8.png)
![](https://pbs.twimg.com/media/FEjKmjFVkAQY8Mn.jpg)
![](https://pbs.twimg.com/media/FEjONKJUUAMVgYS.jpg)
Note that there were 2 genomes in Wuhan and 15 out of the 91 genomes from Sichuan before 2021 carrying intermediate between A and B lineage, with sampling outside the market generally returning much greater diversity than inside the market.
Then, there is an increase in the amt of S seropositivity in sera collected in France in December 23 2019, which would have required the first widespread transmission of SARS-CoV-2 in france beginning at 09/2019--with substantial amt of infection well
Another clue came from italy, which have shown consistent qRT-PCR positivity from wastewater (this is not the november claim with just one sample. this is consistent PR-PCR positivity) since 18/12/2019, which once again
Similarily, consistent detection of SARS-CoV-2 have been reported in Brazil between 27/11/2019 and 11/12/2019. Note that A lineage have been found just south of the location being sampled, and is found in South america in general.
In deed, this would have been consistent with SARS-CoV-2 having a tMRCA in November 17 2019, which is consistent with WIV and WIBP as the origin, with the first outbreak happening in Wuchang, in stead of with the Huanan market as the origin.
In deed, before the more widespread control on the narrative for SARS-CoV-2 origins were established in China, there were reports of contact tracing for first case as early as in 17 November 2019, which is once again earlier than the market, but would have
Considering the extreme censorship of early cases in China, and the existence of consistent
In deed, much higher diversity exist outside of the market than inside the market for the earliest
Certain florida cases also have an onset date at Jan 1 2020.
There are other possible points before, but they are dubious at best. The first confirmed PCR positivity in Italy and Brazil pushes the arrival date of SARS-CoV-2 at these 2 countries (A, possibly A or B) at 27/11/2019.
https://t.co/nUBbhzXisU
![](https://pbs.twimg.com/media/FEj4iEAVEAc_IDU.png)
Very similarly, this happened as well. as many as 4/5 times the active cases were simply removed (not recognized) before the beginning of 2020, simply because they are not epimediologically linked to the Huanan seafood market--you aren't going to find
![](https://pbs.twimg.com/media/FEj6VKaVIAI-mN-.png)
More from Daoyu
https://t.co/QnMiOrdNhx
https://t.co/ZK2vfwYEFj
in fact, adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures. P681 guards the cardin-weintraub motif against cleavage in
cell lines.
https://t.co/vytn6YVRYQ
https://t.co/fjg6ZXc2KN
the FCS is perfectly stable in anything that isn't VERO E6 classic or 293T-ACE2. anything that had TMPRSS2 and grown in trypsin-free media stably maintains the FCS.
https://t.co/NUrJ8AndTx
in fact, the PRRARS, as opposed to other mutated cleavage sites--even the "perfect" H5CS--confers the greatest infectivity in CaLu-3 cells.
https://t.co/TVXC4QOjt0
in fact, even P681R or S686G changes were less fit in CaLu-3 compared to PRRA virus--the P681R virus show either no difference or is slightly less effective compared to the P681 virus, and the S686G virus
https://t.co/DNtIR17r4S
https://t.co/nDNNw9OaWd
![](https://pbs.twimg.com/media/FAtZkQoVkAUFHz_.jpg)
@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017. https://t.co/Vh4dKy6rvI there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of
@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things
@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.
@pathogenetics @ggronvall https://t.co/2679qQiA0P
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
https://t.co/sxm17Eu9hV
https://t.co/FtjifSGItc
PRRA is highly purified in the CaLu-3 cell line when FBS is added to inhibit trypsin. Stu lied about that in his defense on the FCS identity. https://t.co/GzM7uA6vup the Gallaher article contain glaring
@WisdomRebel Mistakes and is completely impossible as HKU9 + RaTG13 only lead to a frameshift inactivated S and not a furin cleaved S.
https://t.co/gmDXK9OluN
The serological test the WIV claimed to be negative in Shi’s addendum is the exact same test they claimed positive in 2012 in the phD
@WisdomRebel thesis, and Stu once again lied about it. https://t.co/Uiy8U9BTYp significant evidence in the form of contaminated SRA datasets suggest that the DEFUSE grant have been funded in China, as the full-length HKU4 is not a part of the 2016-2019 grant, but is found before the start
@WisdomRebel Date of the 2020-2024 grant of June 2020. The WIV also contained several HKU3-like Coronaviruses, particularly in mice in an 2017 GEO experiment, which is consistent with the published HKU3 and batified mice experiment in the DEFUSE document (evidence from an already conducted
@WisdomRebel Experiment). Either DEFUSE or fast-tracked form of the 2020-2024 grant in 2019 would have lead to full-length Sarbecovirus clones being used, leading to SARS-CoV-2 in the lab—they used HKU4 in stead of HKU5 for the MERS spike experiment indicating that backbone sequence distance
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
![](https://pbs.twimg.com/media/EvloxDFVkAA3qbW.jpg)
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
![](https://pbs.twimg.com/media/Evlr5vaVkAEE8sX.jpg)
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