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Saved by @Daoyu15

@WisdomRebel https://t.co/iHKwUoNLJ5

https://t.co/sxm17Eu9hV

https://t.co/FtjifSGItc
PRRA is highly purified in the CaLu-3 cell line when FBS is added to inhibit trypsin. Stu lied about that in his defense on the FCS identity. https://t.co/GzM7uA6vup the Gallaher article contain glaring

 @AngloScot2 @WisdomRebel Mistakes and is completely impossible as HKU9 + RaTG13 only lead to a frameshift inactivated S and not a furin cleaved S.
https://t.co/gmDXK9OluN
The serological test the WIV claimed to be negative in Shi’s addendum is the exact same test they claimed positive in 2012 in the phD
@AngloScot2 @WisdomRebel thesis, and Stu once again lied about it. https://t.co/Uiy8U9BTYp significant evidence in the form of contaminated SRA datasets suggest that the DEFUSE grant have been funded in China, as the full-length HKU4 is not a part of the 2016-2019 grant, but is found before the start
@AngloScot2 @WisdomRebel Date of the 2020-2024 grant of June 2020. The WIV also contained several HKU3-like Coronaviruses, particularly in mice in an 2017 GEO experiment, which is consistent with the published HKU3 and batified mice experiment in the DEFUSE document (evidence from an already conducted
@AngloScot2 @WisdomRebel Experiment). Either DEFUSE or fast-tracked form of the 2020-2024 grant in 2019 would have lead to full-length Sarbecovirus clones being used, leading to SARS-CoV-2 in the lab—they used HKU4 in stead of HKU5 for the MERS spike experiment indicating that backbone sequence distance
@AngloScot2 @WisdomRebel Can not be too high for CoV chimeras, requiring the 4991 backbone or full-length 4991 to be used. In addition, https://t.co/sxm17Eu9hV the 201801-202102 grant of the WIV fits perfectly to DEFUSE since this is consistent with neither the Understanding risk of bat coronavirus
@AngloScot2 @WisdomRebel Emergence grant nor the EID hotspots in southeast asia grant.

https://t.co/YwSTyD3N8B

https://t.co/ySjXCH0k2V
In addition, the claim that the first infection was connected to the wet market is incorrect since the WHO report and the Zenodo-cell article used a resolution
@AngloScot2 @WisdomRebel Of cases that were far too low (which resulted in assigning cases to the wrong side of the yantgtze river) and have intentionally omitted the headquarters of the WIV https://t.co/w7d6bwbAn9 in Wuchang. In addition, the first patient on the WHO report went only to a supermarket in
@AngloScot2 @WisdomRebel Jiangxia, not any wet markets, and it just happened that the RT-mart he visited https://t.co/khQSq8ULAp is in Jiangxia, right next to the BSL-4 building of the WIV.
@AngloScot2 @WisdomRebel In addition, in contradiction to the Stu’s another claim that 4991 is “sequenced only after 2020”, the full-length sequence of 4991 was already discussed in 2018, and the S protein show significant divergence from RaTG13 when compared against SARS-CoV Urbani. https://t.co/VI7I3PwJoK
@AngloScot2 @WisdomRebel https://t.co/ejV0sIlzj5
@AngloScot2 @WisdomRebel https://t.co/ELNPYGVn00
In addition, other anomalies are found in the RBD, especially that of T403 and D501.
@AngloScot2 @WisdomRebel https://t.co/sxsPdd3kBd
@AngloScot2 @WisdomRebel https://t.co/bJma8XExej
In addition, evidence in the form of out-of-whack substitution ratios was found in the RBD of RaTg13, indicating that accelerated evolution in the form of 5-Fluorouracil usage was present in the S protein—which they then go on to compensate in a completely
@AngloScot2 @WisdomRebel Unnatural way in the S2 and the ORF1b using loads and loads of dS changes, throwing the ratios of dS/dN out of whack in both locations. https://t.co/J2YFuLKOqY
@AngloScot2 @WisdomRebel https://t.co/KmOFPkqo1H
@AngloScot2 @WisdomRebel The CGG-CGG pair is at the end point of RNA editing and can not be mutated in the CoV system except for very rare circumstances of RdRp error—it mutates at a rate that is less than 1/10 the rate others can, and no immediately ZAP-beneficial mutation lead to a dS on the CGG pair.
@AngloScot2 @WisdomRebel If you count this low rate of mutation that is possible in the CGG-CGG pair, it is under neutral to slightly positive selection, and not negative or purifying selection. https://t.co/jDy6RoYdSM as for the multi-market hypothesis? Sichuan had loads and loads (1/4 of all sequences)
@AngloScot2 @WisdomRebel That had only T8782C but not C28144T, four of such genomes were found in early Wuhan as well. The 8782/28144 distinction in Wuhan appears in-host before other changes begin to show, so there is no need of two separate introductions for A and B lineage. It happened in humans.
@AngloScot2 @WisdomRebel The FCS PRRARS is highly purified in CaLu-3 cell cultures. Any change to the AA gives significantly reduced infectivity. Therefore, a canonical FCS of RRARR will simply mutate and revert into PRRAR within just 3 passages in the CaLu-3 cell line.
@AngloScot2 @WisdomRebel https://t.co/53dG1S7LnL
@AngloScot2 @WisdomRebel https://t.co/pNwWQ8a7jy
@AngloScot2 @WisdomRebel And “when clear mismatches occur, we will introduce appropriate human-specific cleavage sites and evaluate growth potential in VERO and HAE cultures”. The PRRA is most conserved upon conjugate gradient selection with CaLu-3 as main passage cells and VERO E6 for stock preparation https://t.co/c7UNQR1cnY
@AngloScot2 @WisdomRebel between passages. https://t.co/nRTIU93WGP
@AngloScot2 @WisdomRebel https://t.co/Ff9XAc4ao3
In deed, CaLu-3 have 1/4 O-linked glycosylation that inhibits cleavage upon P681, saving the heparan sulfate binding peptide without compromising TMPRSS2 usage or immunogenicity. VERO E6 had even more O-linked glycosylation and HS binding as it have even
@AngloScot2 @WisdomRebel More uncleaved fractions with the PRRA compared to CaLu-3, and show a more pronounced advantage with PRRARS compared to RRRARS.
@AngloScot2 @WisdomRebel https://t.co/UpOM1kGzTK
@AngloScot2 @WisdomRebel In addition to the extremely high stability of PRRA in CaLu-3 and it's lack of stability in-vivo, some of the earliest patients in Wuhan harbored, by digital PCR and nanopore sequencing, large deletions across the QTQTN and SPRRARS https://t.co/YPHooePHko that are specific to
@AngloScot2 @WisdomRebel VERO E6 cell cultured SARS-CoV-2 strains--indicating that some of the quasispecies from the original cell culture were held over into the very first patients of SARS-CoV-2, strongly supporting the hypothesis that the PRRARS sequence being the result of composite selection
@AngloScot2 @WisdomRebel pressure generated from serial passage in CaLu-3 with stock preparation and storage in VERO E6 between passages, standard laboratory practices when it comes to the serial cell passage of viruses, which may also be employed if a transmissible vaccine (respiratory transmission with
@AngloScot2 @WisdomRebel no pathogenesis in aged WT BalB/C mice).

More from Daoyu

Daoyu
Daoyu
@Daoyu15
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get


A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from

The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals

Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.

The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
SCIENCE
Daoyu
Daoyu
@Daoyu15
@stevenemassey Only a sample is there. This cell line don’t have NGS. But it was consistent with a cell culture, and the RBD of the virus is not suitable for binding to the ACE2 found there. The ACE2 is same as Ra4331 and Ra9479.

And the RBD have a T403 that prevents infection of intestinal cells (hACE2 intestinal organoids, infection is restored upon T403R, but weaker than SARS-CoV-2) or lung cells (R.affinis primary, with supplemented hACE2. Infection is restored upon T403R).


Also that there exist no Sarbecovirus RBD with a D501.
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Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
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Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.

https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV

https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.

Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.

The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
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Daoyu
Daoyu
@Daoyu15
@JackDempsey2_2 @pathogenetics @ggronvall

@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017. https://t.co/Vh4dKy6rvI there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of

@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things

@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.

@pathogenetics @ggronvall https://t.co/2679qQiA0P
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
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More from All

Ankur Patel
Ankur Patel
@AnkurPatel59
FEW USEFUL CHARTINK SCANNERS :

This Scanner will be More than Enough for any Swing / Positional Trader to find Good Stocks.

Few Important Chart ink Scanners which can help you to find Setups and boost up your Trading Account : 🧵

Do Retweet If you If it Useful

1. Momentum Stocks

This Scanner can be helpful for Short term Swing Trades . you can easily find Stocks which are in Momentum.

Stocks which are up by 20 % in 5 Days
Stocks which are up by 30 % in 30 Days
Stocks which are up by 30 % in 30 Days

2. Volume Spike

We always Look for Price and Volume Confirmation for Breakout Trading. This scanner will help you to find the Stocks Which has given a move with Good Volume . You can keep this Stock in Radar and catch the Next move in them :

3 . Reversal Scan

Stocks which are Down by 20 % + in 5 days

When Market Corrects for sometime , You can find Stocks where selling is Exhausted and they can make a Good Reversal move .

4. Reversal Scan - Stock Down continues 5 days .

If you Know the Concept of 3-5 Days move . you might catch few stocks from this scanner. Always try and Pick stocks which are in strong momentum , you have more Chances of Getting Right in Reversal Trade.
TRADING SCREENERS , TRADING SCREENER , ALL
Khatvaanga
Khatvaanga
@khatvaanga
Starting my 2nd thread on Sringeri Parampara. This will cover the Jagadgurus after Sri Vidyaranya to Sri Shivabhinava Nrisimha Bharati.

It is really very tough to match the brilliance and achievements of Vidyaranya. After whom came 15 - Chandrashekara Bharati [1386-1389].

The next Acharya was Nrisimha Bharati [1389-1408]. Harihara raya found a town for this Acharya - Narasimhapuram. Acharya helped the king by guiding him in political affairs. He also initiated the kind with mahamantras for spiritual development.

Subsequent Acharyas were
15-Puroshottama Bharati [1408 - 1448]
16-Shankara Bharati[1448 - 1455]
17-Chandrasekhara Bharati II [1455 - 1464]
18- Nrisimha Bharati II [1464 - 1479]
19-Puroshottama Bharati II [1479 - 1517]

20-Ramachandra Bharati [1517 - 1560]. Sadashiva Raya was emporer of Vijayanagara during this time.
21-Nrisimha Bharati III [1560 - 1573]
22-Nrisimha Bharati IV [1573 - 1576]
23-Nrisimha Bharati V [1576 - 1600]

24-Abhinava Nrisimha Bharati [1600-1623]. When he visited Malahanikareswara temple for first time he noticed there was no Vinayaka there so he took piece of turmeric and drew outline of Ganesha on a pillar and offered puja.
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𝕍𝕠𝕪𝕒𝕘𝕖𝕣...
@V0YAGERTWEETS
Ｇａｕ Ｍａｔａ Ｉｎ Ｖｅｄｉｃ
Ｃｕｌｔｕｒｅ

It is said that the one who bears the dust thrown from the hoof of Gaumata on his head, as if taking a bath in the water of the teerth sthalam and getting rid of all sins.


Ved and all other Hindu scriptures venerate the cow. The Vedic dictionary, Nighantu, gives amongst other synonyms of 'Gau' [ or cow] the words 'Aghnya', 'Ahi', and 'Aditi'.

Yaska the vedic decoder and commentator on Nighantu, defines these as-

Aghnya —— the one that ought not to be killed
Ahi —— the one that must not be slaughtered.
Aditi —— the one that ought not to be cut into pieces.

These three names of cow signify that this is an animal which should not to be put to tortures. These words appear frequently throughout the Veda in context of the cow.

But in due course the falsehood of killing cows spread far and wide and they became even more deep rooted when western scholars with their half baked knowledge of Sanskrit transliterated these interpretations of commentaries of Sayanacharya ,
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anirudhsinh parmar
anirudhsinh parmar
@anirudhparmar68
Read this. U may get it for free.
Short straddle :
What is ?? Sell an atm call and put.
Instrument: mostly bnf and nf.
Now there are versions of it.
1. Best version :
Enter around 9.15-20 and exit around 3.20. No adjustment nothing.
1/n.


If u r doing it on zero leverage(meaning u have full capital of around bnf's notional contract,i.e. 40000*25 = 10lacs), then u r most likely going to make better than fd or anything like that.
Simple rules : friday,monday, tuesday do for weekly. Wednesday and thrusday next week

So, gamma moves can't impact much.
Another better version of mahesh chander kaushik is to do it for monthly expiry.
Bigger premiums and never hav to worry about 200-300 points sudden moves.
2. 2nd version : have combined stoploss of premiums and re-entry criteria. Example :

Let say 40000 straddle is at 600. Sell at 600 and hav combined sl at 650. Many algos are providing this. U can even place stoploss in % terms. Like, if sold straddle at 400 and placed 10% sl, means if 440 combined premium touch, it will be stoploss.
Let say, in morning,

U sold straddle at 600 and it sl hit at 650. Now, u placed reentry at 600 with same sl. This time may be linear decay happens and u may cover morning's loss and make some profit.
3rd version : stoplosses at each leg and trail sl to cost type.
Example , 40000 straddle at 400.
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Buzz Chronicles
Buzz Chronicles
@buzz_chronicles
@SaveToBookmarks Hello! Pleasure is mine. Original tweet by @buzz_chronicles: "@SaveToBookmarks Salut! Pleasure is mine. Original tweet by @AchuthArora: "@FitF...". Thanks! ✌

Hola! Of course! Original tweet by @SaveToBookmarks: "@buzz_chronicles @AchuthArora @FitFounder @rattibha Cool!

I saved this tweet fo...". Enjoy 👌
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itrade(DJ)
itrade(DJ)
@ITRADE191
Here is the detailed information of about strategy,

Entry time : 9.30 - 10
Exit : Upto you

Strategy :
Sell weekly ATM CE & PE at almost equal price
For ex : Sell Nifty 17250 CE at 50 and Nifty 17250 PE at 48 so it will become short straddle

Buy monthly ATM or near ATM CE & PE at matching price (5-10 points diff should be fine) as hedge
For ex : Buy Nifty 17250 CE at 150 and Nifty 17300 PE at 155 so it will become long straddle.

Weekly Short straddle + Monthly Long straddle

Adjustment:

• If you feel slightly bullish/bearish then no adjustment needed
• If Nifty moves 50 up or down and sustain then just simply roll up or down half of qnty and keep rest of the quantity. If market goes up or down further then roll up or down rest.

• If Nifty moves one side or about to breach BEP then add equal amount of lot. This can balance tested side. For ex: If I bought 17300 CE 4 lot as hedge then add 4 lot so you will have only one side risk . Note: Remember to keep SL at cost price (only for additional lot bought)

Capital requirement:
2.2 L for 4 lot
Adjustment requires additional capital upto 1L

Note : High risk high reward strategy, please don’t execute until you fully understand. Kindly do paper trading or backtest.
RR : 1:1
POP : 45-55%
OPTIONSLEARNINGS , ALL
WORLD OF SANATAN DHARMA
WORLD OF SANATAN DHARM...
@world_sanatan
LESSER KNOWN DETAILS ABOUT THE BATTLE OF PRATAPGAD - THIS YOU ALL SHOULD READ

In the 1659 Adilshah sent Afzalkhan with the army of 75000 soldiers to destroy Chhatrapati Shivaji Maharaj with his empire. He was the main soldier, warrior and minister in Adilshahi Sultanate.


He was the Subhedar of Wai province.

He was very powerful physically. His height was 7.3 ft. due to same he was very popular as a strong man from Adilshahi Sultanate.

Whole India was not daring to fight with Adilshahi due to Afzal Khan.

Badi Begum Sahiba sent him to kill Chhatrapati Shivaji on any cost.

Voluntary he decided to destroy Chhatrapati Shivaji’s empire with him. He started his operation against Chhatrapati Shivaji from "wai". He destroyed many temples like as Tulja Bhavani Temple.

He destroyed so many villages, killed villagers to torture Chhatrapati Shivaji.

DETAILED VIEW- Running commentary

1. Chhatrapati Shivaji chose Pantaji as a lawyer to communicate with Afzal Khan. Pantaji was very sharp minded, who did his job very nicely.

2. He presented Chhatrapati Shivaji is fearing Afzal Khan and he wanted to invite him in his kingdom near Pratapgad.

3. Pantaji succeed in the communication with Afzal Khan and he brought him as per the planning of Chhatrapati Shivaji.
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Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
Santkumaras-The four adorable kids of the Universe.

The Kumaras r the four sages who roam the Universe as children from the Puranic texts of Hinduism named,Sanaka, Sanatana,Sanandana& Sanatkumara.They r described as the first mind-born creations &sons of the creator God Brahma.


Brahma,who had received the knowledge of Puranas from the Universal being(God Himself), imbibed this to his 4 Kumaras.
Sanat Kumaras then taught the Puranas to Narada.Narada transmitted it to Krishna Dwaipayana who in turn scripted them in monumental epic documents of 18 Puranas.

The Vishnu Purana is recorded in two parts,the Vishnu Purana & Naradiya Purana.The teachings of Sanaka of the Kumara brothers r contained in the Naradiya Purana which is also divided in two parts, the first part containing the teachings of Sanaka &second part,teachings of others.


Born from Brahma's mind,4 Kumars took lifelong vows of celibacy against their father's wishes.They r said to wander throughout materialistic &spiritualistic universe without any desire but with the aim to teach.All 4 brothers studied Vedas from their Childhood&travelled together.


The Bhagwata Purana lists the Kumaras among the 12 Mahajanas(great devotees or bhaktas) who although being eternally liberated souls from birth, still became attracted to the devotional service of Vishnu from their already enlightened state.
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Ramsay Rippers \U0001f354
Ramsay Rippers 🍔...
@RamsayRippers
🧵EMA + VWAP STRATEGY

Every trader has a go-to strategy that works best for them. My trading is all about keeping it simple stupid and revolves around two indicators - Exponential moving averages and vwap.

Here's the strategy that I utilize on nearly 80% of my trades.

👇

What are Exponential Moving Averages?

EMA's are a type of moving average that places a greater weight on more recent data points.

8EMA = Exponential Moving Averages of the last 8 candle closing prices.

EMA's adjust with respect to chart time frame.

What is VWAP?

Volume Weighted Average Price - A key intraday indicator in my trading that offers the true average price based on price & volume.

Calculation: (Price x # of shares traded) / total shares traded

EMA's I use

- 8
- 21
- 50

While I do utilize the 50EMA, the 8 & 21 EMA are most important for this strategy. Some people use the 9 and 20 which are essentially the same thing. REMEMBER: EMA'S adjust with respect to time frames.

A+ Set Up Criteria - (What I look for)
STRATEGY
Anu Satheesh \U0001f1ee\U0001f1f3
Anu Satheesh 🇮🇳...
@AnuSatheesh5
Eri Katha Ramar Temple
Maduranthakam

Eri Katha Ramar temple is located at Maduranthakam near Chengalpattu of Tamil Nadu. Beautiful Kodanda Ramar of about 8ft holding Ma Sita's hand & with Lakshmana. This temple story is connected with Sri Ramanujar.
@LostTemple7


It was here Periya Nambigal performed Pancha Samskaaram to Ramanujar under Magizham tree.
Sri Ramanujar was on his way from Kanchipuram to meet Sri Periya Nambigal at Sri Rangam to become his disciple. Sri Periya Nambigal also started towards Kanchipuram to meet Ramanujar.


They both met here at Madurantakam.
Small Mandapam where Ramanujar undertook Pancha Samskaaram is seen in the temple. Separate sannidhi is for Periya Nambigal & Ramanujar & this is the only temple where Ramanujar is seen as a Grahastha (married person) and not in Sanyasa kolam.


Story says about an incident which occurred during British rule. The Madurantakam lake bund was about to breach, due to heavy rain & village will be flooded if it happened. Dt collector was Cl. Lionel Blaze who took efforts to protect the bund & asked for the piles of stones


in the temple to protect it. But it was meant for Janakavalli ( sita) Thayar sannidhi which faced shortage of fund. Lionel Blaze prayed Sri Ramar to save the bund and if so he will provide fund to construct.
That night he saw in the lightning, Sri Rama & Lakshmana walked along
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