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Saved by @Daoyu15

https://t.co/TVXC4QOjt0
https://t.co/QnMiOrdNhx
https://t.co/ZK2vfwYEFj
in fact, adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures. P681 guards the cardin-weintraub motif against cleavage in

 cell lines.
https://t.co/vytn6YVRYQ
https://t.co/fjg6ZXc2KN
the FCS is perfectly stable in anything that isn't VERO E6 classic or 293T-ACE2. anything that had TMPRSS2 and grown in trypsin-free media stably maintains the FCS.
https://t.co/NUrJ8AndTx
in fact, the PRRARS, as opposed to other mutated cleavage sites--even the "perfect" H5CS--confers the greatest infectivity in CaLu-3 cells.
https://t.co/TVXC4QOjt0
in fact, even P681R or S686G changes were less fit in CaLu-3 compared to PRRA virus--the P681R virus show either no difference or is slightly less effective compared to the P681 virus, and the S686G virus
https://t.co/DNtIR17r4S
https://t.co/nDNNw9OaWd
show significantly less entry compared to PRRAR/S681 virus.https://t.co/9HCxNjyNAC similarily, both CaLu-3 and HELA-hACE2 show significantly reduced viral entry upon mutation of the FCS.
https://t.co/tAP9OQ9FYl
P681 is favored specifically becuse of the cardin-weintraub motif it leaves behind (pRRaRs(O-hexNAc)) compared to R681.
https://t.co/LqKsreg1tu
in deed, even the optimized RRRKR site fares LESS well compared to the unmodified PRRAR site for CaLu-3.
https://t.co/6XFCMUPUqe
once agaiP681 and P681 with RRARS in particular, optimizes CaLu-3 cell entry for the SARS-CoV-2 S.
https://t.co/9HX97A9T5S

https://t.co/H7UqiJ15v9
The final piece of the puzzle: it turned out that CaLu-3 cells DO glycosylate the S1-S2 in the presence of P681, which is necessary for all the previous mechanisms to function—in deed the live SARS-CoV-2 virus on CaLu-3
Cells leads to a significant fraction of S proteins that have not been cleaved, whereas almost no S remain uncleaved on pseudoparticles produced on HEK293T cells. https://t.co/UDOU3kDGlT
These uncleaved fractions indicate that intact cardin-weintraub motifs are formed during
CaLu-3 cell culture, and that these motifs are formed as the result of interference of furin cleavage in the cells by P681-mediated glycosylation of T678 and S685.
https://t.co/qbaazFZTIF

https://t.co/9O3WLoNiY0
In addition, G614 eliminated these motifs by allowing complete cleavage of the S protein, which is why it will not form during cell passage in CaLu-3. As O-linked glycosylation in The live virus is incomplete, it can not perform any of the proposed immunoevasive function
As claimed by Anderson et al, as it (with fraction <25% on CaLu-3) will not be able to shield most of the S trimers on virion (only <1/64 of the S trimers will be fully shielded given the fraction observed on CaLu-3) against Antibody or T cell recognition on the S1-S2 junction,
Whereas the intact Cardin-Weintraub motifs formed on each virion (~50 Heparan Sulfate binding sites formed for 100 S trimers per virion), alongside with the O-glycan epitopes ares sufficient to both confer an selective advantage (inhibition of cleavage in at least 20% of the S,
Binding to Heparan Sulfate proteoglycan coreceptors in culture) in the CaLu-3 cell line and confer an selective disadvantage in the presence of an innate immune system (SIGLEC binding by O-linked HexNAc glycans, PRR recognition of intact cardin-weintraub motif by macrophages) in
Live hosts—if P681 is so critical to shield the virus from the immune system, why it is destroyed in humans by P681R and P681H mutations? If P681 can not arise in passage, why it is so conserved in CaLu-3 cells? It is important to notice that while CaLu-3 cells are used
As a model for airway epithelial cells for passage experiments, viral stock preparation (after each passage) is still only performed in VERO E6 cells. This cell line had a much more pronounced preference of P681 over R681 compared to CaLu-3, especially if TMPRSS2 is expressed in
The cell line—therefore, if virus stocks are regularly prepared between passage experiments, per the PREEMPT proposal, the conjugate potential formed by VERO E6 and CaLu-3 cell lines will fixate P681 on the PRRA insert even faster than CaLu-3 alone, while the CaLu-3 passages
In between completely eliminates any VERO-derived mutations between passages.
In fact, the presence of mutations that both abolish S1-S2 glycosylation (destruction of the P681 residue) and disrupts the Cardin-Weintraub motif in all major human Variants-Of-Concern (B.1.1.7, B.1.617.2,A.23.1) in the form of an additional basic residue appended before the
XBBXBX motif, during the course of immune selection in live human hosts, runs directly against the idea that P681 and cell-specific O-linked glycosylation associated with it could “only be the result of immune selection in the presence of a functional immune system”—in stead it
is the exact opposite, specifically favored in the two PREEMPT-specified cell types (airway epithelial and VERO) and abhorred by immune selection in-vivo. https://t.co/elcdQBF9z3
https://t.co/tAP9OQ9FYl
O-linked glycosylation is only found in uncleaved fractions, which contains an intact XBBXBX Cardin-weintraub Heparan Sulfate binding motif that is favored above furin cleavage specifically in cell cultures. Uncleaved fraction is found both in VERO and CALU-3 cells, which
O-glycosylates the S1-S2 with fractions that are consistent with the uncleaved S in total S for these cell lines.

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Daoyu
Daoyu
@Daoyu15
@VanGennepD @_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 While they are allowed to engage with U.S. scientists, all their Q&A are pre-trained, pre-rehersed and all their speech and demonstration are carefully pre-scripted. You can even hear the long pauses between Shi’s words in all her webinars and interviews, indicative of a script

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 Being handed to her and then read. No word that are outside of the script or are bad to the CCP’s official narrative (they are carefully trained and rehearsed before doing any webinars, meetings or interviews to U.S. scientists) are allowed in these engagements—they are allowed

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 To engage but are not free. If they disclose anything that hurts the CCP narrative they and their families are disappeared.
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Daoyu
Daoyu
@Daoyu15
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Daoyu
Daoyu
@Daoyu15
@danwalker9999 https://t.co/o8zqouq0HB
Bias, however, is an important problem in the WHO data. Also, an important problem seen in the points being scattered around is that they seems to be pulled toward the Huanan market—it was likely that it was used as the point of origin for the coordinate

System used. https://t.co/KnPRvIN8uK At this level of imprecision, it becomes impossible ti distinguish the Huanan market, the Wuhan CDC, and the Hankou railway station—the last of which is the main transport hub in Wuhan and the only transport hub reachable from the WIV within


1 transfer on the metro system. Cases “unlinked” were mainly clustered north of the Hankou station—which was one of the major exits through the hankou station and one of the transfer stations next to the Hankou railway station. Even connor reed, which is on the east of the

Yangtze river, supposedly have onset in November 2019 (whistleblower case #2), was connected to the WIV directly—it was on the same metro line, line 8, as the WIV. As he is an education worker, he need to commute to Wuchang where the universities are—exposing him to the WIV

Every time he takes line 8 to commute. https://t.co/5TIqOjt2p1
In addition to enforced ascertainment bias toward the market with a retrospective case search that specifically targeted the immediate surrounding of Huanan market, symptomology bias with lineage B created an
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Daoyu
Daoyu
@Daoyu15
@jbloom_lab It seems that it is a deeply cultured, but in deed ancestrally derived, sequence of SARS-CoV-2, that have many of the VERO cell specific mutations and deletions already in.

but it is not likely a clinical specimen from China because of the USA/WA-1 base which is not in Wuhan.

It had T22657C, T3346C, A21562C and G487T. all of which is in RaTg13 but not in WuHu-1. also T1963C and T22963C in BANAL-52. not all of the reads were H655Y and del I68-T76.

And it is actually clustered closely to the A/North America lineage, which shouldn't have appeared in a Chinese lab. also not there in Wuhan either.

also only one of the SRRs harbored T18060C it seemed.
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Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.

https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV

https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.

Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.

The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
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𝕍𝕠𝕪𝕒𝕘𝕖𝕣...
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Ｇａｕ Ｍａｔａ Ｉｎ Ｖｅｄｉｃ
Ｃｕｌｔｕｒｅ

It is said that the one who bears the dust thrown from the hoof of Gaumata on his head, as if taking a bath in the water of the teerth sthalam and getting rid of all sins.


Ved and all other Hindu scriptures venerate the cow. The Vedic dictionary, Nighantu, gives amongst other synonyms of 'Gau' [ or cow] the words 'Aghnya', 'Ahi', and 'Aditi'.

Yaska the vedic decoder and commentator on Nighantu, defines these as-

Aghnya —— the one that ought not to be killed
Ahi —— the one that must not be slaughtered.
Aditi —— the one that ought not to be cut into pieces.

These three names of cow signify that this is an animal which should not to be put to tortures. These words appear frequently throughout the Veda in context of the cow.

But in due course the falsehood of killing cows spread far and wide and they became even more deep rooted when western scholars with their half baked knowledge of Sanskrit transliterated these interpretations of commentaries of Sayanacharya ,
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@VIBHU_Tweet
RANI NAIKI DEVI AND THE BATTLE OF KASAHRADA

Mohammad Ghori, the young Ghurid Prince conquered Ghaznavids in Afghanistan in the year 1173 CE. He conducted some successful raids into the Indian territory, which even Mahmud Ghazni & the Persians couldn't do.


First&foremost,Ghori invaded states of Multan &Uch in India.After this he turned to southern states of Gujarat &Rajputana.Everybody knows this about Ghori,but what most pple r unaware of is the defeat he suffered at hands of Chalukyan Queen Naiki Devi in 1178 Battle of Kasahrada.


When he marched southwards, his first target was the prosperous fortified town of Anhilwara Patan.
It was the capital of Chalukyas(also known as Solankis of Gujarat) which was established by Vanraj of Chapotkata Dynasty in 8th century.


American historian Tertius Chandler states that it was the 10th largest city in the world at that time. The history of Chalukyas has been written by many historians, but the valour of the Great Queen Naiki Devi remains unsung.


Born to the Kadamba King Paramardin of Goa,Naiki Devi was well trained in sword fighting, horse riding, strategy & every other subject of Statesmanship. She was married to King Ajaypala of Gujarat who sat on throne in 1171CE. Unfortunately,the King died within 4 yrs of his rule.
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itrade(DJ)
itrade(DJ)
@ITRADE191
Here is the detailed information of about strategy,

Entry time : 9.30 - 10
Exit : Upto you

Strategy :
Sell weekly ATM CE & PE at almost equal price
For ex : Sell Nifty 17250 CE at 50 and Nifty 17250 PE at 48 so it will become short straddle

Buy monthly ATM or near ATM CE & PE at matching price (5-10 points diff should be fine) as hedge
For ex : Buy Nifty 17250 CE at 150 and Nifty 17300 PE at 155 so it will become long straddle.

Weekly Short straddle + Monthly Long straddle

Adjustment:

• If you feel slightly bullish/bearish then no adjustment needed
• If Nifty moves 50 up or down and sustain then just simply roll up or down half of qnty and keep rest of the quantity. If market goes up or down further then roll up or down rest.

• If Nifty moves one side or about to breach BEP then add equal amount of lot. This can balance tested side. For ex: If I bought 17300 CE 4 lot as hedge then add 4 lot so you will have only one side risk . Note: Remember to keep SL at cost price (only for additional lot bought)

Capital requirement:
2.2 L for 4 lot
Adjustment requires additional capital upto 1L

Note : High risk high reward strategy, please don’t execute until you fully understand. Kindly do paper trading or backtest.
RR : 1:1
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Siddharth Karamcheti
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How can we use language supervision to learn better visual representations for robotics?

Introducing Voltron: Language-Driven Representation Learning for Robotics!

Paper: https://t.co/gIsRPtSjKz
Models: https://t.co/NOB3cpATYG
Evaluation: https://t.co/aOzQu95J8z

🧵👇(1 / 12)


Videos of humans performing everyday tasks (Something-Something-v2, Ego4D) offer a rich and diverse resource for learning representations for robotic manipulation.

Yet, an underused part of these datasets are the rich, natural language annotations accompanying each video. (2/12)

The Voltron framework offers a simple way to use language supervision to shape representation learning, building off of prior work in representations for robotics like MVP (https://t.co/Pb0mk9hb4i) and R3M (https://t.co/o2Fkc3fP0e).

The secret is *balance* (3/12)

Starting with a masked autoencoder over frames from these video clips, make a choice:

1) Condition on language and improve our ability to reconstruct the scene.

2) Generate language given the visual representation and improve our ability to describe what's happening. (4/12)

By trading off *conditioning* and *generation* we show that we can learn 1) better representations than prior methods, and 2) explicitly shape the balance of low and high-level features captured.

Why is the ability to shape this balance important? (5/12)
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@SaveToBookmarks Namaste! There you go! Original tweet by @buzz_chronicles: "@SaveToBookmarks Namaste! You can find it here. Original tweet by @AchuthArora: ...". Enjoy 👌

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...". See You soon! 👉
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Make them irrelevant > Hold a grudge

Holding a grudge is the easy thing to do.

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A grudge puts poison in your body & rewires your subconscious mind.

You consume the poison & destroy your reality while your attacker sleeps like a baby.
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The kingdom of Sindh was ruled by its last Hindu king Raja Dahir during the 8th century. When Muhammad-bin-Qasim invaded Sindh, he decimated Sindh in his savage bloodlust. The chronicles of his advent are described in the Chachnama.


2/n
The Chachnama describes in detail how the Arabs led by butcher Qasim conquered Sindh. It tells the terrible tale of bravery, treachery, & carnage marking the entry of Islamic conquest of India’s Hindus.


3/n
When Muhammad-bin-Qasim attacked Sindh, Dahir had collected an army of 50,000 horses and marched from Brahamanabad to Rawar to face the invader. Qasim had an army that numbered in the tens of thousands with camels & horses.

4/n
Earlier Arab Alafis - Syeds from Prophet Mohammed’s own family had fled from Mecca with 500 men to Sindh and Raja Dahir had given them asylum. Trusting these Muslims would prove to be the fatal mistake for the downfall of Sindh & pave the way for Islamic invasion.

5/n
On an “auspicious day” in A.D. 711, fixed by astrologers, Mohammed Bin Qasim started for Sindh at the head of the Iraqi, Syrian, and other Arab soldiers. His horses and camels were disguised to look like lions and elephants. Rebel Jats also joined Qasim’s army.
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Professor
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Some ofthe biggest breakouts occur after Volatility Contraction, the analogy is that more a spring is pressed, higher it jumps whenever it gets released.

There is a VCP Scanner on chartink (Not mine). This may also be used to find potential Breakouts.
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We should stop confusing what someone was doing when we became aware of them with all they are capable of, interested in, or ever hope to be

Let folks have their many talents, interests and gifts. Life is far more fun with variety, loves.

A lot of folks have come to know me as an activist & I’m grateful that folks care to know me at all.

But I wasn’t born in 2014. I was a whole teacher, executive, policy person, speaker, arts and culture lover, reader, writer, woman of faith, fashion and more before 4 yrs ago 🤷🏾‍♀️

We rightfully complain that marginalized people are not allowed to be fully human.

But we internalize and transfer our oppression daily. It’s a smog. We all breathe it in & act it out.

And then tell WoC “girl ain’t you supposed to be a _______? Why you doing ____?”

Can I live?

And don’t go reading anything personal into this-this isn’t about me necessarily and it’s no subtweet (I try hard not to do that.)

I’ve just been observing that behavior more and more lately. Especially when it comes to marginalized folks.

Evolution should be our aspiration.

“Can’t knock the hustle” should be our anthem.

As long as someone isn’t bringing active and continual harm, why can’t they explore their many sides?
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THE EVOLUTION OF OUR NATIONAL FLAG (1857-1947)

The INDIAN NATIONAL FLAG is not just a flag! It's a testimony of patriotism and seamless sacrifice. A symbol representing one of the oldest civilizations on this planet.

Let's hope what happened yesterday never repeats!

(Thread)


After the revolt of 1857, the first Indian flag was designed. The design was based on western heraldic standards with "Union Jack" in the upper-left quadrant and a "Star of India" enclosed in the royal crown in the middle of the right half.(1)


The flag was a symbol of the direct imperial rule in India.(2)

In 1904 the first Indian flag which stood for India's independence from the British was devised. The design was provided by sister Nivedita, an Irish disciple of Swami Vivekananda.(3)


The flag was red and yellow with an image 'Vajra' (weapon of god Indra). The phrase 'Vande Mataram' was written in Bengali on it. The red colour and the yellow colour signified freedom and victory while the symbol of vajra stood for strength.(4)
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