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Saved by @Daoyu15

https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get

 A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Where the extremely low error-ridden cross-contaminating molecule were amplified with mutagenesis happening on each step—especially between the initial RT-PCR and sequencing PCR process, which cross contamination have happened. Notice that any extra Pro or Cys on the highly
Conserved N protein sequences are lethal to the virus—especially the extra Cys which will become a nucleophile and a Ribonuclease in the budded virion in an extracellular environment, degrading the genome and inactivating the virus immediately. These Cys will also cause the N to
Dimerize and consequently lose it’s ability to bind RNA or mediate virion assembly in the first place. While the Pro will break a conserved secondary structure in the C-terminal domain and prevent the N from folding correctly. These are not found anywhere else and are obviously
Sequencing errors. Especially since these samples came from cross-contamination without a corresponding positive oral or anal swab (they didn’t get a single swab that is positive).
M1, they cite as having “hemorrhage” claimed to be Coronavirus symptoms, are anything BUT coronavirus symptoms. https://t.co/3a8nqY6rOz “Open in new tab
X-ray tests revealed a large area of shadow in the left lung of pangolin 2-Lishui, suggestive of pneumonia” but
“we have not found coronaviruses in these pangolins by meta-transcriptomic and PCR methods.”. Notice the PLT- in the Pestivirus-infected pangolin samples. Pestivirus is seen in the SRA of M1, SRX7756769 (107267359 spots as in the Xiao et al article). The Pestivirus is seen
In all PRJNA573298 sample lungs, and it causes interstitial pneumonia and gives the same kind of CT scan and blood test result as in the 06/22 article. This sample also contained a locally circulating CCoV (Canine Coronavirus, often infects zoo/rescued solitary animals due to
It’s ubiquitous nature in most human-associated environments) strain with reads that weren’t rolling circles as the GD-1 reads and are mostly related to the Canine Coronavirus isolates circulating in dogs in GZ.
Coronavirus infections can not generate rolling circles as they does not encode RNA ligases, an enzyme that is necessary to create a true CircRNA. (What they claim to be CircRNA reads from CoV infections in other papers aren’t true CircRNA but are Partially duplicated RNA due to
The Coronavirus RdRp template-switching to a location before it’s current location. This RNA is still linear. However, the Rolling Circles corresponds to two hotspots of M13 primers seen in the amplicons for nsp3 and for the 3’-end of the genome. This imply that they were
Remnants of cDNA cloning, stemming from DNA that were circularized without a vector backbone.)
Canine Coronavirus and FIPV (alphacoronavirus 1) infections also generate very similar symptoms, and will result in positive cross-reactive NP in the immunofluorescence screening. Even the right GEO profile provide they remove it from the final SRA. Finally, the ACE2 expression
“Lowest in the lungs” claimed in this paper is at odd with the 06/22 paper claim that “ACE2 and TMPRSS2 is most highly expressed in the lungs”. This suggest that the GEO profile of the 06/22 article associated with A22/SRX8582289 have been fabricated or were derived from hACE2
Mouse. The hemorrhage seen in M1 is consistent with DYPV(Flavivirus/pestivirus related to BVDV) which is seen in the metagenome, while the pneumonia could be either be the result of DYPV-related Pestivirus, from the Sendai (murine respirovirus), from the Canine Coronavirus or
From any of the non-Coronavirus (Colitivirus or Murine Respirovirus) agents found in PRJNA573298.
M1 have Murine Respirovirus.
https://t.co/ZJnLSxwe5o
SeV is in the same family as human Parainfluenza virus. It causes bilateral ground-glass opacity.
https://t.co/NeY2ajwsDo
The blood tests—pestivirus once again fully explains all the blood changed observed. When a Parainfluenza virus is also present, the blood test become indistinguishable.
Another snRNA-seq say ACE2 and TMPRSS2 are barely exiressed in the lungs and are never coexpressed.
Weaker than the kidneys—this is again at odds with the SCAU claim that even negative pangolins have lung>>kidney for ACE2. Both publication say that Kidney is the highest ACE2 expressing organ in all pangolins where lung expression is negligible. The 06/22 article is the only
One that claim that all pangolins including negative samples, have lung ACE2 >> Kidney. This indicate that they have Spiked all of them with K12-hACE2 mouse as it is in disagreement of all previous or later pangolin transcriptomes.
“(Individual number: L08) as a positive control”—and guess what L08 actually is—Human AND Mice!
L08: Lung08.

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@jbloom_lab It seems that it is a deeply cultured, but in deed ancestrally derived, sequence of SARS-CoV-2, that have many of the VERO cell specific mutations and deletions already in.

but it is not likely a clinical specimen from China because of the USA/WA-1 base which is not in Wuhan.

It had T22657C, T3346C, A21562C and G487T. all of which is in RaTg13 but not in WuHu-1. also T1963C and T22963C in BANAL-52. not all of the reads were H655Y and del I68-T76.

And it is actually clustered closely to the A/North America lineage, which shouldn't have appeared in a Chinese lab. also not there in Wuhan either.

also only one of the SRRs harbored T18060C it seemed.
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https://t.co/iNCdLY1Pzn
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@VanGennepD @_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 While they are allowed to engage with U.S. scientists, all their Q&A are pre-trained, pre-rehersed and all their speech and demonstration are carefully pre-scripted. You can even hear the long pauses between Shi’s words in all her webinars and interviews, indicative of a script

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 Being handed to her and then read. No word that are outside of the script or are bad to the CCP’s official narrative (they are carefully trained and rehearsed before doing any webinars, meetings or interviews to U.S. scientists) are allowed in these engagements—they are allowed

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 To engage but are not free. If they disclose anything that hurts the CCP narrative they and their families are disappeared.
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Daoyu
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@franciscodeasis https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.

https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV

https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.

Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.

The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
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Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
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@NotTheMacAnon1
If you shine light of high enough energy on to a metal, electrons will be emitted from the metal.
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Asbj\xf8rn St\xf8ylen
Asbjørn Støylen
@strain_rate
1/ #LBBB generates often a classical pattern on #EchoFirst. The pattern is very distinctive in Tissue Doppler of the septum.


The classical pattern arises from the time lapse of the activation and relaxation of the two walls, creating a pattern of interaction due to a sequence temporal imbalances of the tension between the two walls.


2/ As the septum is activated first, it contracts (shortening - septal flash) without activation of the lateral wall, which stretches. This generates slower pressure build up than a normal IVC, which then is prolonged.


3/ During ejection, the LV volume decreases, so both walls shorten. Tension, however, declines first in the septum, as this was activated first. This leads to a tension imbalance, so the lateral wall continues to shorten, the more relaxed septum stretches.


4/ The stretching of the septum builds up an elastic tension in the septum, which is released as recoil, when tension declines in the lateral wall, showing a post systolic shortening in the septum which is due to elasticity.
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Robbie Andrew
Robbie Andrew
@robbie_andrew
"NO LONGER BEST IN THE WORLD"
UNEP's new Human Development Index includes a new (separate) index: Planetary pressures-adjusted HDI (PHDI). News in Norway is that its position drops from #1 to #16 because of this, while Ireland rises from #2 to #1.
Why?

https://t.co/aVraIEzRfh


Check out Norway's 'Domestic Material Consumption'. Fossil fuels are no different here to Ireland's. What's different is this huge 'non-metallic minerals' category.
(Note also the jump in 1998, suggesting data problems.)
https://t.co/5QvzONbqmN


In Norway's case, it looks like the apparent consumption equation (production+imports-exports) for non-metal minerals is dominated by production: extraction of material in Norway.
https://t.co/5QvzONbqmN


And here we see that this production of non-metallic minerals is sand, gravel and crushed rock for construction. So it's about Norway's geology.
https://t.co/y6rqWmFVWc


Norway drops 15 places on the PHDI list not because of its CO₂ emissions (fairly high at 41st highest in the world per capita), but because of its geology, because it shifts a lot of rock whenever it builds anything.
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Rebecca \U0001f983 "Stay the Fuck Home" \U0001f983 Watson
Rebecca 🦃 "Stay the Fu...
@rebeccawatson
So it turns out that an organization I thought was doing good work, the False Memory Syndrome Foundation (associated with Center for Inquiry, James Randi, and Martin Gardner) was actually caping for pedophiles. Uhhhh oops?


Since this, bizarrely, turned out to be one of my longest videos ever (??) here's a quick thread to sum it up for those of you like myself with short attention spans. 1/10

In the '90s the False Memory Syndrome Foundation was founded to call attention to the problem of adults suddenly "remembering" child abuse that never actually happened, often under hypnosis. Skeptics like James Randi & Martin Gardner joined their board. 2/10

A new article reveals that the FMSF was founded by parents who had been credibly and PRIVATELY accused of molestation by their now-adult daughter. They publicized the accusation, destroyed the daughter's reputation, and started the foundation. 3/10

The FMSF assumed any accused pedo who joined was innocent, saying "We are a good-looking bunch of people, graying hair, well dressed, healthy, smiling; just about every person who has attended is someone you would surely find interesting and want to count as a friend" 😬 4/10
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Marina Amaral
Marina Amaral
@marinamaral2
And since today is International Day of Women and Girls in Science, let's highlight a few of them - just a few women among thousands of others who helped shape the world we live in today.

Thread.


Dorothy Hodgkin discovered the structure of insulin after 36 years of work.

"I was captured for life by chemistry and by crystals."


Sophia Jex-Blake fought for women's rights to study medicine. She was involved in founding two medical schools for women.

"It seemed discreditable to Great Britain that all her daughters who desired a University education should be driven abroad to seek it."


Alice Augusta Ball developed the "Ball Method", the most effective treatment for leprosy during the early 20th century. She was 23.


Ada Lovelace is regarded as the first to recognize the full potential of computers and as one of the first computer programmers.

“That brain of mine is something more than merely mortal; as time will show.”
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Panniyur Sri Varahamurthy Temple Palakkad, Kerala
#KeralaTemples 🚩

Panniyur Sri Varahamurthy Temple is located in Pattambi Taluk of Palakkad District. This is believed to be the first temple in Kerala, where Prathishta was done by Parasurama

Story says that, Parashurama after


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As Earth is saved in Varaha Avatar, this temple is famous to solve land related issues.

Many people conduct Bhoomi puja here, which is very special here. They bring sand from around the temple which is used for pooja and then has to be returned to the same spot 👇


from where it was taken.
It is also believed that Perumthachan, the legedary craftsman has abandoned his tools here.
Om Shri Varaha Murthaye Namah
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RANI GAIDINLIU:THE LESSER KNOWN FREEDOM FIGHTER

Rani Gaidinliu joined the freedom struggle at 13 &was sentenced to life imprisonment at 16."We r free people—the White should not rule over us.”Thus calling out to her Zeliangrong tribesmen was the voice of 13yr-old Rani Gaidinliu.


She joined the freedom struggle in the present-day Northeast India. Today, her struggle is lost in history as most of her countrymen wouldn’t even have heard her name. B'coz our history textbooks tell us that only Mahatma Gandhi &Pandit Nehru got India its Independence.


At 16 yrs of age, this valiant girl was sentenced to life imprisonment for leading a movement to drive out the colonial rulers from Manipur and Nagaland. Nevertheless, she was released in 1947 after India’s independence and was given the title ,"Rani of Nagas".


EARLY LIFE AND HERAKA MOVEMENT

Gaidinliu belonged to the Rongmei clan of the Zeliangrong tribe in the Tamenglong district of western Manipur. Born on January 26, 1915, she was fifth among eight children.


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Ashta Siddhi

Ashta Siddhi is mentioned in Patanjali Yog sutra and can be obtained by very few through countless years of practice and devotion to all eight limbs of Ashtanga Yoga.

Siddhi can loosely be translated as accomplishment or a special power or unusual skill.


The eight Siddhis are:

1. Anima: Reducing one’s physical self to the size of an atom.

2. Mahima: Expanding one’s physical self to infinite level

3. Garima: Making one’s physical self so heavy as immovable by others

4. Laghima: Becoming almost weightless


5. Prapti: Being able travel anywhere in the Universe

6. Prakamya: Can know & understand what is going on in one’s mind. Can understand other person’s min.

7. Ishitva: Possessing title of Bhagwan

8. Vashitva: A seeker can subdue any person/thing

(Hanuman Jayanti is coming)


Hanumanji not only possessed all eight siddhis, but was also blessed by Sita mata as “Ashta Siddhi Nau Nidhi Ke Daata”.

When Hanumanji first reaches to Lanka on his mission to find Sita, he makes use of anima so as to be discrete in enemy territory.


He uses it again when he approaches Sita mata for the first time – reducing his size to that of a schoolboy, to avoid scaring her. He uses mahima to outwit & overpowers
the demons.
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THE LESSER KNOWN MAHARANA OF MEWAR:RANA KUMBHA
Kumbalgarh Fort is one of most spectacular sites of India,atop a hill abt 3600ft above sea lvl in the https://t.co/LkjmzzBhE2's second largest wall is surrounding it.A fort wall second only to Great wall of China,still not so famous.


This longest wall of India stretches to almost 36Km and encompasses a few hills, many step wells and over 360 temples and palaces. Birth place of the Great Maharana Pratap, this fort is named after another Great Maharana of Mewar, who is not given his due in the History.


Builder of many architectural marvels like the Kumbhalgarh Fort, an erudite scholar, a patron of arts, music, culture & a great warrior was the Great Maharana Kumbha. Yet we don't know much about him. Let's get to know him a little.


Maharana Kumbha was the undefeated King of Mewar from 1433-1468CE.He never lost any battles &kept Mewar out of the reach of Islamic Invaders till the time he was alive. Born on Makar Sakranti to Maharana Mokal & Saubhagya Devi, Rana Kumbha was only 13 when he ascended the throne.


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Peter McCormack [Jan/3\u279e\u20bf \U0001f511\u220e]
Peter McCormack [Jan/3...
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1/ So here is a thread on how I turned $32,000 into $1.2m and back to pretty much zero (once taxes are paid).

Just note, I am not bitter or salty in any way at all, the last 2 years have been an amazing ride - travelled the world, been wealthy, been poor.

2/ Dec '16, my advertising agency folded, I had a little bit of money left and I put $32k into Bitcoin and Ether. As it started to go up I diversified into everything, Monero, Dash, this that, any crap - even Ripplecoin. Everything just kept going up.

3/ By March I think I had around $300k and $500k by the summer. I used to take 25% out but towards the end of the summer I got greedy and put it all back in and by December it was $1.2m.

4/ Thinking I was an absolute genius I decided to start a bunch of businesses. As silly as it sounds I had this goal of making $5m as I wanted to buy Bedford Town Football Club and get them in the league, and as Crypto was going up forever I needed 6 months.

5/ So:
- Trading (income 1)
- Podcast (income 2)
- Mining (income 3)
- Mining pool (income 4)
- Consulting (income 5)

Yes - all of the above as a one-man army :)
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