BC SCIENCE
Saved by @Daoyu15

https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get

 A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Where the extremely low error-ridden cross-contaminating molecule were amplified with mutagenesis happening on each step—especially between the initial RT-PCR and sequencing PCR process, which cross contamination have happened. Notice that any extra Pro or Cys on the highly
Conserved N protein sequences are lethal to the virus—especially the extra Cys which will become a nucleophile and a Ribonuclease in the budded virion in an extracellular environment, degrading the genome and inactivating the virus immediately. These Cys will also cause the N to
Dimerize and consequently lose it’s ability to bind RNA or mediate virion assembly in the first place. While the Pro will break a conserved secondary structure in the C-terminal domain and prevent the N from folding correctly. These are not found anywhere else and are obviously
Sequencing errors. Especially since these samples came from cross-contamination without a corresponding positive oral or anal swab (they didn’t get a single swab that is positive).
M1, they cite as having “hemorrhage” claimed to be Coronavirus symptoms, are anything BUT coronavirus symptoms. https://t.co/3a8nqY6rOz “Open in new tab
X-ray tests revealed a large area of shadow in the left lung of pangolin 2-Lishui, suggestive of pneumonia” but
“we have not found coronaviruses in these pangolins by meta-transcriptomic and PCR methods.”. Notice the PLT- in the Pestivirus-infected pangolin samples. Pestivirus is seen in the SRA of M1, SRX7756769 (107267359 spots as in the Xiao et al article). The Pestivirus is seen
In all PRJNA573298 sample lungs, and it causes interstitial pneumonia and gives the same kind of CT scan and blood test result as in the 06/22 article. This sample also contained a locally circulating CCoV (Canine Coronavirus, often infects zoo/rescued solitary animals due to
It’s ubiquitous nature in most human-associated environments) strain with reads that weren’t rolling circles as the GD-1 reads and are mostly related to the Canine Coronavirus isolates circulating in dogs in GZ.
Coronavirus infections can not generate rolling circles as they does not encode RNA ligases, an enzyme that is necessary to create a true CircRNA. (What they claim to be CircRNA reads from CoV infections in other papers aren’t true CircRNA but are Partially duplicated RNA due to
The Coronavirus RdRp template-switching to a location before it’s current location. This RNA is still linear. However, the Rolling Circles corresponds to two hotspots of M13 primers seen in the amplicons for nsp3 and for the 3’-end of the genome. This imply that they were
Remnants of cDNA cloning, stemming from DNA that were circularized without a vector backbone.)
Canine Coronavirus and FIPV (alphacoronavirus 1) infections also generate very similar symptoms, and will result in positive cross-reactive NP in the immunofluorescence screening. Even the right GEO profile provide they remove it from the final SRA. Finally, the ACE2 expression
“Lowest in the lungs” claimed in this paper is at odd with the 06/22 paper claim that “ACE2 and TMPRSS2 is most highly expressed in the lungs”. This suggest that the GEO profile of the 06/22 article associated with A22/SRX8582289 have been fabricated or were derived from hACE2
Mouse. The hemorrhage seen in M1 is consistent with DYPV(Flavivirus/pestivirus related to BVDV) which is seen in the metagenome, while the pneumonia could be either be the result of DYPV-related Pestivirus, from the Sendai (murine respirovirus), from the Canine Coronavirus or
From any of the non-Coronavirus (Colitivirus or Murine Respirovirus) agents found in PRJNA573298.
M1 have Murine Respirovirus.
https://t.co/ZJnLSxwe5o
SeV is in the same family as human Parainfluenza virus. It causes bilateral ground-glass opacity.
https://t.co/NeY2ajwsDo
The blood tests—pestivirus once again fully explains all the blood changed observed. When a Parainfluenza virus is also present, the blood test become indistinguishable.
Another snRNA-seq say ACE2 and TMPRSS2 are barely exiressed in the lungs and are never coexpressed.
Weaker than the kidneys—this is again at odds with the SCAU claim that even negative pangolins have lung>>kidney for ACE2. Both publication say that Kidney is the highest ACE2 expressing organ in all pangolins where lung expression is negligible. The 06/22 article is the only
One that claim that all pangolins including negative samples, have lung ACE2 >> Kidney. This indicate that they have Spiked all of them with K12-hACE2 mouse as it is in disagreement of all previous or later pangolin transcriptomes.
“(Individual number: L08) as a positive control”—and guess what L08 actually is—Human AND Mice!
L08: Lung08.

More from Daoyu

Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
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Daoyu
Daoyu
@Daoyu15
@stevenemassey Only a sample is there. This cell line don’t have NGS. But it was consistent with a cell culture, and the RBD of the virus is not suitable for binding to the ACE2 found there. The ACE2 is same as Ra4331 and Ra9479.

And the RBD have a T403 that prevents infection of intestinal cells (hACE2 intestinal organoids, infection is restored upon T403R, but weaker than SARS-CoV-2) or lung cells (R.affinis primary, with supplemented hACE2. Infection is restored upon T403R).


Also that there exist no Sarbecovirus RBD with a D501.
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Daoyu
Daoyu
@Daoyu15
@AngloScot2 @WisdomRebel https://t.co/iHKwUoNLJ5

https://t.co/sxm17Eu9hV

https://t.co/FtjifSGItc
PRRA is highly purified in the CaLu-3 cell line when FBS is added to inhibit trypsin. Stu lied about that in his defense on the FCS identity. https://t.co/GzM7uA6vup the Gallaher article contain glaring

@WisdomRebel Mistakes and is completely impossible as HKU9 + RaTG13 only lead to a frameshift inactivated S and not a furin cleaved S.
https://t.co/gmDXK9OluN
The serological test the WIV claimed to be negative in Shi’s addendum is the exact same test they claimed positive in 2012 in the phD

@WisdomRebel thesis, and Stu once again lied about it. https://t.co/Uiy8U9BTYp significant evidence in the form of contaminated SRA datasets suggest that the DEFUSE grant have been funded in China, as the full-length HKU4 is not a part of the 2016-2019 grant, but is found before the start

@WisdomRebel Date of the 2020-2024 grant of June 2020. The WIV also contained several HKU3-like Coronaviruses, particularly in mice in an 2017 GEO experiment, which is consistent with the published HKU3 and batified mice experiment in the DEFUSE document (evidence from an already conducted

@WisdomRebel Experiment). Either DEFUSE or fast-tracked form of the 2020-2024 grant in 2019 would have lead to full-length Sarbecovirus clones being used, leading to SARS-CoV-2 in the lab—they used HKU4 in stead of HKU5 for the MERS spike experiment indicating that backbone sequence distance
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Daoyu
Daoyu
@Daoyu15
@VanGennepD @_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 While they are allowed to engage with U.S. scientists, all their Q&A are pre-trained, pre-rehersed and all their speech and demonstration are carefully pre-scripted. You can even hear the long pauses between Shi’s words in all her webinars and interviews, indicative of a script

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 Being handed to her and then read. No word that are outside of the script or are bad to the CCP’s official narrative (they are carefully trained and rehearsed before doing any webinars, meetings or interviews to U.S. scientists) are allowed in these engagements—they are allowed

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 To engage but are not free. If they disclose anything that hurts the CCP narrative they and their families are disappeared.
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Daoyu
Daoyu
@Daoyu15
https://t.co/SvP0tfbDki


https://t.co/k9mmL9e5kn


https://t.co/7fR13yb3qJ


https://t.co/EXerlaiG9u


https://t.co/iNCdLY1Pzn
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AKASH BHAT
AKASH BHAT
@AKASHBH95801446
EXPLANATION OF THE EXISTENCE OF DARK MATTER AND DARK ENERGY !

What Is Dark Energy ?

More is unknown than is known. We know how much dark energy there is because we know how it affects the universe's expansion. Other than that, it is a complete mystery.

But it is an important mystery. It turns out that roughly 68% of the universe is dark energy. Dark matter makes up about 27%. The rest - everything on Earth, everything ever observed with all of our instruments, all normal matter - adds up to less than 5% of the universe.

This diagram reveals changes in the rate of expansion since the universe's birth 15 billion years ago. The more shallow the curve, the faster the rate of expansion. The curve changes noticeably about 7.5 billion years ago, when objects in the universe began flying apart as


a faster rate. Astronomers theorize that the faster expansion rate is due to a mysterious, dark force that is pulling galaxies apart.

WHAT OUR SANATAN DHARMA SAYS IN THIS CONTEXT LET’S TAKE A LOOK

The verses of Taittiriya Aranyaka say that only one pada of the Lord

is this manifested universe and the remaining three padas are outside it.

According to the observations and realizations of ancient seers, nearly eighty percent of this universe is yet of unknown nature which remains inactive during the process of creation (rather, evolution).
SCIENCE
Bert Hubert
Bert Hubert
@PowerDNS_Bert
Recently I learned something about DNA that blew my mind, and in this thread, I'll attempt to blow your mind as well. Behold: Chargaff's 2nd Parity Rule for DNA N-Grams.
If you are into cryptography or reverse engineering, you should love this.
Thread:


DNA consists of four different 'bases', A, C, G and T. These bases have specific meaning within our biology. Specifically, within the 'coding part' of a gene, a triplet of bases encodes for an amino acid


Most DNA is stored redundantly, in two connected strands. Wherever there is an A on one strand, you'll find a T on the other one. And similarly for C and G:

T G T C A G T
A C A G T C A

(note how the other strand is upside down - this matters!)


If you take all the DNA of an organism (both strands), you will find equal numbers of A's and T's, as well as equal numbers of C's and G's. This is true by definition.
This is called Chargaff's 1st parity rule.
https://t.co/jD4cMt0PJ0


Strangely enough, this rule also holds per strand! So even if you take away the redundancy, there are 99% equal numbers of A/T and C/G * on each strand *. And we don't really know why.
This is called Chargaff's 2nd parity rule.
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Kris Postadjian
Kris Postadjian
@KrisP55
A Thread about Famous Armenian Inventors and what we have contributed to the world. So the next time someone wants to say we are unimportant, you can rest easy knowing Armenians have contributed some of the greatest inventions to mankind. Here is a handful but there's more.


Raymond Damadian: Armenian-American Physician and inventor of the first MRI machine. MRI is a medical imaging technique used in radiology to form pictures of the anatomy and the physiological processes of the body. Raymond created the first MRI in 1977.


Artem Mikoyan: One of the most famous engineers in aviation. Mikoyan along with Gurevich created one of the most famous Jet lines in history, the MiG. MiG jets directly competed with American Lockheed Martin F Series planes and were among the first to surpass the speed of sound.


Hovannes Adamian: Most notably laid the foundations of color television with his Tri-Color Principle. His work would go on to become the framework that is used to create Color-TV that we see today. He is recognized as one of the founders of color television.


Emik Avakian: An inventor who unfortunately was disabled, but that didn't stop him from contributing work that would help people similar to his condition. Notable contributions are the automatic wheelchair which dismiss the action of hand rolling and a breath operated typewriter.
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Matthew Salganik
Matthew Salganik
@msalganik
Announcing the 2021 Summer Institutes in Computational Social Science. #SICSS is for grad students, post-docs & beginning faculty. Free for participants. @chris_bail and I are happy to tell you about all 20 locations. https://t.co/wolhtZIMc5 [thread]


SICSS brings together social scientists & data scientists interested in computational social science for 1-2 week events with intensive study & collaborative research.

Topics covered include automated text analysis, web scraping, non-probability sampling, digital experiments, ethics & much more.

All education materials created for SICSS are available open-source so that people who are unable to participate can still learn. You can also use them in your teaching. Here are materials from previous years:

Because of COVID, all SICSS locations will be online only in 2021.
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Thomas Oliver \U0001f41d
Thomas Oliver 🐝...
@thomasroliveruk
Neonicotinoids and why they need to stay banned 🐝

#SciComm #ScienceTwitter #SaveTheBees #ClimateEmergency #ClimateCrisis


Neonicotinoids were banned by the EU in 2018 following multiple scientific studies which proved their severe detrimental impact on pollinators. Effects from neonics include reduced immune function, reduced foraging capabilities, and impaired cognition in bees.


The government has approved neonics for emergency use as a seed treatment on sugar beet. Yields have been impacted by beet yellows, transmitted by aphids. They argue that, because sugar beet does not flower and will not attract pollinators, the risk from neonics is acceptable.


Since the ban on neonics in 2018, the infection rate of beet yellows has increased year on year. Aphid populations have been proliferating due to mild winters, with no agrochemicals to solve the problem.

Sounds like a good case for neonics, right?


But this is the start of a vicious cycle. Climate change isn't going away. Winters will continue to get milder, resulting in the need for more and more pesticides to combat growing pest problems. Inevitably, this won't be the last time neonics are authorised for emergency use.
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💬 I often get Q’s like these:

How did you get your first customers for WIP? How did you grow BetaList’s traffic? Etc.

Makers are looking to reverse-engineer success. I see it everywhere.

I don’t think it works that way and the answers to those questions are mostly useless. 💥

I have built dozens of different products over the last couple of years. The vast majority failed. 😭

Surely if I know the answers to these questions, but still fail over and over again, these answers aren’t that useful. 🤷‍♀️

So what’s a better question to ask? 🤔

99.9% of the questions I receive are about the products that did well. In a way that makes sense, because we quickly forget about those that didn’t succeed.

🧠 This is known as survivorship bias.

Focusing on what survived, while ignoring what made it survive in the first place.

The real question, what you really want to know, is this:

What makes @WIP, @BetaList, and to some extent @AllStartupJobs succeed where my countless other attempts failed?

What separates a failed product 👎 from a successful product 👍?

Honestly, I don’t know. I wish I did.

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Same is true when we make products. We don’t know upfront what will work. But once we see an inkling of a product that does have potential, it’s not that hard to spot.
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Will Hulsey was the undisputed king of the animal attack pulp cover. You name it, he'd paint it attacking you in a pool of stagnant water.


Very little is known about Will Hulsey, but he worked on a number of men's pulp magazines in the 1950s and early 1960s including Man's Life, True Men, Guilty, Trapped and Peril.


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As a result the 1950s saw a raft of men's pulp magazines being published to tap into this market - almost 200 different titles!
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दधीचि ऋषि को मनाही थी कि वह अश्विनी कुमारों को किसी भी अवस्था में ब्रह्मविद्या का उपदेश नहीं दें। ये आदेश देवराज इन्द्र का था।वह नहीं चाहते थे कि उनके सिंहासन को प्रत्यक्ष या परोक्ष रुप से कोई भी खतरा हो।मगर जब अश्विनी कुमारों ने सहृदय प्रार्थना की तो महर्षि सहर्ष मान गए।


और उन्होनें ब्रह्मविद्या का ज्ञान अश्विनि कुमारों को दे दिया। गुप्तचरों के माध्यम से जब खबर इन्द्रदेव तक पहुंची तो वे क्रोध में खड़ग ले कर गए और महर्षि दधीचि का सर धड़ से अलग कर दिया।मगर अश्विनी कुमार भी कहां चुप बैठने वाले थे।उन्होने तुरंत एक अश्व का सिर महर्षि के धड़ पे...


...प्रत्यारोपित कर उन्हें जीवित रख लिया।उस दिन के पश्चात महर्षि दधीचि अश्वशिरा भी कहलाए जाने लगे।अब आगे सुनिये की किस प्रकार महर्षि दधीचि का सर काटने वाले इन्द्र कैसे अपनी रक्षा हेतु उनके आगे गिड़गिड़ाए ।

एक बार देवराज इन्द्र अपनी सभा में बैठे थे, तो उन्हे खुद पर अभिमान हो आया।


वे सोचने लगे कि हम तीनों लोकों के स्वामी हैं। ब्राह्मण हमें यज्ञ में आहुति देते हैं और हमारी उपासना करते हैं। फिर हम सामान्य ब्राह्मण बृहस्पति से क्यों डरते हैं ?उनके आने पर क्यों खड़े हो जाते हैं?वे तो हमारी जीविका से पलते हैं। देवर्षि बृहस्पति देवताओं के गुरु थे।

अभिमान के कारण ऋषि बृहस्पति के पधारने पर न तो इन्द्र ही खड़े हुए और न ही अन्य देवों को खड़े होने दिया।देवगुरु बृहस्पति इन्द्र का ये कठोर दुर्व्यवहार देख कर चुप चाप वहां से लौट गए।कुछ देर पश्चात जब देवराज का मद उतरा तो उन्हे अपनी गलती का एहसास हुआ।
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Adam McKay
Adam McKay
@GhostPanther
1) My Stan Lee story happened when I was in 5th grade. I was visiting NYC with my Dad and had read the address for Marvel in the comics I read constantly. I begged my Dad to take me and to his credit he did. And guess what? It was just an office.

2) We were down in the lobby and I was kind of crushed. I don’t know what I expected, The Thing and the Yancy Street Gang to be sitting around smoking cigars? Anyway, my Dad was taking a beat to figure where we were going next and a guy came up to us.

3) He was wearing a white shirt and tie and said to Dad “Is he disappointed because. The Marvel offices were just offices?” My Dad said yes and then the guy who had gray around his temples and a mustache said “hold on a second” and opened one of those office mailboxes with a key.

4) He then handed me a thick stack of EVERY SINGLE MARVEL COMIC COMING OUT THE NEXT MONTH. “Here you go. Keep reading Marvel comics” he said and then walked off. I left in a daze and about 15 minutes later it hit me “Gray around the temples, mustache... That was Stan Lee!”

5) Later when I wrote on the Ant Man movie I told Kevin Feige the story, the year, look of the guy etc and Kevin said “That’s exactly the kind of thing Stan would do and he would have been there then. That was him.” Rest In Peace Stan Lee and thank you for the comics.
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Jaya_Upadhyaya
Jaya_Upadhyaya
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SRI KUMARIAMMAN, KANYAKUMARI (TN)
#bharatmandir #navratri2021

Created by Maharshi Parasurama and also one among the 108 Shakthi Peethas in the world. The name Kanyakumari stands for Kanya(Virgin) and Kumari(girl).
Devi took this form to vanquish the demon Banasura
@GunduHuDuGa


Once, Banasura(grand son of Mahabali) obtained a boon from Shiva that he could be vanquished only by a virgin.
So, Devi took the form of a kumari and started meditating with the desire to marry Shiva at Suchindram.
Pleased Shiva fixed the midnight hour for marriage with Devi.


Devarshi Narada knew that the marriage would end the chance of killing Banasura. So, he assumed the form of a cock and crowed. Shiva returned back to Suchindram thinking that it was dawn.
Angered, Devi cursed that all food and articles meant for wedding turn into sand and shells


When Banasura heard about Devi’s beauty, he tried to take her by force. Devi, then, killed him and granted the wish of Devas to remain there always protecting them.
The murti of Devi stands with a rosary in hand. Her diamond nose-ring is said to be visible even from the sea.


Once an ancient mariner mistook it for a lighthouse and sailing towards it, his ship wrecked upon the Kanya Kumari rocks. In order to prevent such a tragedy from happening again, the eastern door of the shrine is opened only on five special occasions throughout the year.
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