https://t.co/Xe5xFdtDfO
https://t.co/e3RBxj0ly3
https://t.co/cJlCMqyP2v
https://t.co/5n5TK67iKB
https://t.co/r5JIAWB9Oq

More from Billy Bostickson 🏴👁&👁 🆓

1/ Directed Evolution and Serial passage of RNA Viruses
A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories

2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
https://t.co/BfR0B2Hwsf


3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
https://t.co/G3mIptI54H


4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
Automation

5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
https://t.co/wdKyXghRYe
Advances in the directed evolution of proteins
https://t.co/1xXTtw1i3i
In vivo continuous directed evolution
https://t.co/xKRVSjVvo4
Tip from the Monkey
Pangolins, September 2019 and PLA are the key to this mystery
Stay Tuned!


1. Yang


2. A jacobin capuchin dangling a flagellin pangolin on a javelin while playing a mandolin and strangling a mannequin on a paladin's palanquin, said Saladin
More to come tomorrow!


3. Yigang Tong
https://t.co/CYtqYorhzH
Archived: https://t.co/ncz5ruwE2W


4. YT Interview
Some bats & pangolins carry viruses related with SARS-CoV-2, found in SE Asia and in Yunnan, & the pangolins carrying SARS-CoV-2 related viruses were smuggled from SE Asia, so there is a possibility that SARS-CoV-2 were coming from
Zhengli Shi and Deyin Guo in 2015
Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Increase or Decrease Replication and Pathogenesis
https://t.co/YMlsqFOvZp


Deyin Guo 2016:
Molecular mechanisms of coronavirus RNA capping and methylation
https://t.co/xdEU5goLB6


Deyin Guo 2012
Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2′-O-methyltransferase activity of nsp10/nsp16 complex
https://t.co/x30jPYFDTa


Deyin Guo 2013
Structure-function Analysis of SARS Coronavirus RNA Cap Guanine-N7 Methyltransferase.
https://t.co/NG57b85hcs


Deyin Guo 2016
Identification and Characterization of a Ribose 2’-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales
https://t.co/zZ5V7erseD
1. Thread: RaTG13 Real or Fake?
A Norwegian Scientist @Vehuardo recently claimed RaTG13 is an "authentic virus"
https://t.co/tAXMNTwdhA
This failed to take into account the work of our DRASTIC colleagues & other published analyses.
This thread is a polite attempt to educate.

2.
https://t.co/tw606vgHwp
which says RaTG13 doesn't make sense on how it was sequenced, or how the data got so messed up in the lab as to include copy paste mistakes, bat dna, pangolin dna, rice dna, mice dna, & nobody should use it to form conclusions until it's properly vetted

2 (cont)
"sequence is incomplete & provided segment doesn't function. The identified markers suggest partial match basis & actual virus itself as described by the sequence can't functionally exist due to incoherent structures that appear to be the result of sample contamination"


3. RaTG13 integrity is further attacked here:
The Validity of critical pieces of evidence for the natural origin of SARS-CoV-2 is Dubious, and needed to be

4. and here:
Major Concerns on the Identification of Bat Coronavirus Strain RaTG13 and Quality of Related Nature

More from Science

You May Also Like

Ivor Cummins has been wrong (or lying) almost entirely throughout this pandemic and got paid handsomly for it.

He has been wrong (or lying) so often that it will be nearly impossible for me to track every grift, lie, deceit, manipulation he has pulled. I will use...


... other sources who have been trying to shine on light on this grifter (as I have tried to do, time and again:


Example #1: "Still not seeing Sweden signal versus Denmark really"... There it was (Images attached).
19 to 80 is an over 300% difference.

Tweet: https://t.co/36FnYnsRT9


Example #2 - "Yes, I'm comparing the Noridcs / No, you cannot compare the Nordics."

I wonder why...

Tweets: https://t.co/XLfoX4rpck / https://t.co/vjE1ctLU5x


Example #3 - "I'm only looking at what makes the data fit in my favour" a.k.a moving the goalposts.

Tweets: https://t.co/vcDpTu3qyj / https://t.co/CA3N6hC2Lq