https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the

@franciscodeasis chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project https://t.co/HPJhwq7NgH
@franciscodeasis starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".
@franciscodeasis P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!
@franciscodeasis https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
@franciscodeasis Ref.5: "However, inspection of Figure 4 shows that clade B is connected to viruses lacking T8782C and C28144T by single mutational steps via other human isolates, so this explanation requires not only positing two markets with two progenitors differing by just two mutations,
@franciscodeasis but also the exceedingly improbable evolution of one of these progenitors towards the other after it had jumped to humans."
@franciscodeasis in fact, both T8782C without C28144T and C28111T without T8782C have been found in humans. clearly these are impossible to be "two separate markets" as A and B have been found to be connected by single mutations in humans--requiring the exceedingly improbable event of one
@franciscodeasis progenitor evolving toward the other in humans.
@franciscodeasis https://t.co/4gQ4cwKyGk
@franciscodeasis As for ref 4? Why ALL the closest bat-borne backbones for SARS-CoV-2 LACKED the Spike that is necessary for infection of anything that isn't it's own species? RpYN06 had ZC45 S, which infect only R. Pusillus and R. Blythi. RpACE2 have been experimentally confirmed to be not
@franciscodeasis Usable by any RBD/RBM from the clade SARS-CoV or SARS-CoV-2, and could not have been able to recombine with any of these because of physical isolation between R. Pusillus and SARS-CoV-2-like Spike and RBD.
@franciscodeasis https://t.co/3vdIBEBA1k
@franciscodeasis Ref 6? Why the “full-length genomes” claimed by the RaTG15 paper were DISCONTIGUOUS with the RdRp sequences deposited under the title “Origin and cross-species transmission of bat coronaviruses in China” in 13-Aug-2019? https://t.co/gBfRmGcTf4 https://t.co/2E63NmRjTE
@franciscodeasis No other Betacoronaviruses have been isolated from R.blythi.
@franciscodeasis @garyruskin

More from Daoyu

@AngloScot2 @WisdomRebel https://t.co/iHKwUoNLJ5

https://t.co/sxm17Eu9hV

https://t.co/FtjifSGItc
PRRA is highly purified in the CaLu-3 cell line when FBS is added to inhibit trypsin. Stu lied about that in his defense on the FCS identity. https://t.co/GzM7uA6vup the Gallaher article contain glaring

@WisdomRebel Mistakes and is completely impossible as HKU9 + RaTG13 only lead to a frameshift inactivated S and not a furin cleaved S.
https://t.co/gmDXK9OluN
The serological test the WIV claimed to be negative in Shi’s addendum is the exact same test they claimed positive in 2012 in the phD

@WisdomRebel thesis, and Stu once again lied about it. https://t.co/Uiy8U9BTYp significant evidence in the form of contaminated SRA datasets suggest that the DEFUSE grant have been funded in China, as the full-length HKU4 is not a part of the 2016-2019 grant, but is found before the start

@WisdomRebel Date of the 2020-2024 grant of June 2020. The WIV also contained several HKU3-like Coronaviruses, particularly in mice in an 2017 GEO experiment, which is consistent with the published HKU3 and batified mice experiment in the DEFUSE document (evidence from an already conducted

@WisdomRebel Experiment). Either DEFUSE or fast-tracked form of the 2020-2024 grant in 2019 would have lead to full-length Sarbecovirus clones being used, leading to SARS-CoV-2 in the lab—they used HKU4 in stead of HKU5 for the MERS spike experiment indicating that backbone sequence distance
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get


A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from

The Taq.
https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals

Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.

The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
@danwalker9999 https://t.co/o8zqouq0HB
Bias, however, is an important problem in the WHO data. Also, an important problem seen in the points being scattered around is that they seems to be pulled toward the Huanan market—it was likely that it was used as the point of origin for the coordinate

System used.
https://t.co/KnPRvIN8uK At this level of imprecision, it becomes impossible ti distinguish the Huanan market, the Wuhan CDC, and the Hankou railway station—the last of which is the main transport hub in Wuhan and the only transport hub reachable from the WIV within


1 transfer on the metro system. Cases “unlinked” were mainly clustered north of the Hankou station—which was one of the major exits through the hankou station and one of the transfer stations next to the Hankou railway station. Even connor reed, which is on the east of the

Yangtze river, supposedly have onset in November 2019 (whistleblower case #2), was connected to the WIV directly—it was on the same metro line, line 8, as the WIV. As he is an education worker, he need to commute to Wuchang where the universities are—exposing him to the WIV

Every time he takes line 8 to commute. https://t.co/5TIqOjt2p1
In addition to enforced ascertainment bias toward the market with a retrospective case search that specifically targeted the immediate surrounding of Huanan market, symptomology bias with lineage B created an

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The best morning routine?

Starts the night before.

9 evening habits that make all the difference:

1. Write down tomorrow's 3:3:3 plan

• 3 hours on your most important project
• 3 shorter tasks
• 3 maintenance activities

Defining a "productive day" is crucial.

Or else you'll never be at peace (even with excellent output).

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2. End the workday with a shutdown ritual

Create a short shutdown ritual (hat-tip to Cal Newport). Close your laptop, plug in the charger, spend 2 minutes tidying your desk. Then say, "shutdown."

Separating your life and work is key.

3. Journal 1 beautiful life moment

Delicious tacos, presentation you crushed, a moment of inner peace. Write it down.

Gratitude programs a mindset of abundance.

4. Lay out clothes

Get exercise clothes ready for tomorrow. Upon waking up, jump rope for 2 mins. It will activate your mind + body.

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