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https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the

 @franciscodeasis chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project https://t.co/HPJhwq7NgH
@franciscodeasis starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".
@franciscodeasis P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!
@franciscodeasis https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
@franciscodeasis Ref.5: "However, inspection of Figure 4 shows that clade B is connected to viruses lacking T8782C and C28144T by single mutational steps via other human isolates, so this explanation requires not only positing two markets with two progenitors differing by just two mutations,
@franciscodeasis but also the exceedingly improbable evolution of one of these progenitors towards the other after it had jumped to humans."
@franciscodeasis in fact, both T8782C without C28144T and C28111T without T8782C have been found in humans. clearly these are impossible to be "two separate markets" as A and B have been found to be connected by single mutations in humans--requiring the exceedingly improbable event of one
@franciscodeasis progenitor evolving toward the other in humans.
@franciscodeasis https://t.co/4gQ4cwKyGk
@franciscodeasis As for ref 4? Why ALL the closest bat-borne backbones for SARS-CoV-2 LACKED the Spike that is necessary for infection of anything that isn't it's own species? RpYN06 had ZC45 S, which infect only R. Pusillus and R. Blythi. RpACE2 have been experimentally confirmed to be not
@franciscodeasis Usable by any RBD/RBM from the clade SARS-CoV or SARS-CoV-2, and could not have been able to recombine with any of these because of physical isolation between R. Pusillus and SARS-CoV-2-like Spike and RBD.
@franciscodeasis https://t.co/3vdIBEBA1k
@franciscodeasis Ref 6? Why the “full-length genomes” claimed by the RaTG15 paper were DISCONTIGUOUS with the RdRp sequences deposited under the title “Origin and cross-species transmission of bat coronaviruses in China” in 13-Aug-2019? https://t.co/gBfRmGcTf4 https://t.co/2E63NmRjTE
@franciscodeasis No other Betacoronaviruses have been isolated from R.blythi.
@franciscodeasis @garyruskin

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Daoyu
Daoyu
@Daoyu15
@jbloom_lab It seems that it is a deeply cultured, but in deed ancestrally derived, sequence of SARS-CoV-2, that have many of the VERO cell specific mutations and deletions already in.

but it is not likely a clinical specimen from China because of the USA/WA-1 base which is not in Wuhan.

It had T22657C, T3346C, A21562C and G487T. all of which is in RaTg13 but not in WuHu-1. also T1963C and T22963C in BANAL-52. not all of the reads were H655Y and del I68-T76.

And it is actually clustered closely to the A/North America lineage, which shouldn't have appeared in a Chinese lab. also not there in Wuhan either.

also only one of the SRRs harbored T18060C it seemed.
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Daoyu
Daoyu
@Daoyu15
@JackDempsey2_2 @pathogenetics @ggronvall

@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017. https://t.co/Vh4dKy6rvI there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of

@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things

@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.

@pathogenetics @ggronvall https://t.co/2679qQiA0P
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
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Daoyu
Daoyu
@Daoyu15
https://t.co/TVXC4QOjt0
https://t.co/QnMiOrdNhx
https://t.co/ZK2vfwYEFj
in fact, adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures. P681 guards the cardin-weintraub motif against cleavage in

cell lines.

https://t.co/vytn6YVRYQ
https://t.co/fjg6ZXc2KN
the FCS is perfectly stable in anything that isn't VERO E6 classic or 293T-ACE2. anything that had TMPRSS2 and grown in trypsin-free media stably maintains the FCS.

https://t.co/NUrJ8AndTx
in fact, the PRRARS, as opposed to other mutated cleavage sites--even the "perfect" H5CS--confers the greatest infectivity in CaLu-3 cells.

https://t.co/TVXC4QOjt0
in fact, even P681R or S686G changes were less fit in CaLu-3 compared to PRRA virus--the P681R virus show either no difference or is slightly less effective compared to the P681 virus, and the S686G virus
https://t.co/DNtIR17r4S
https://t.co/nDNNw9OaWd
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Daoyu
Daoyu
@Daoyu15
@VanGennepD @_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 While they are allowed to engage with U.S. scientists, all their Q&A are pre-trained, pre-rehersed and all their speech and demonstration are carefully pre-scripted. You can even hear the long pauses between Shi’s words in all her webinars and interviews, indicative of a script

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 Being handed to her and then read. No word that are outside of the script or are bad to the CCP’s official narrative (they are carefully trained and rehearsed before doing any webinars, meetings or interviews to U.S. scientists) are allowed in these engagements—they are allowed

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 To engage but are not free. If they disclose anything that hurts the CCP narrative they and their families are disappeared.
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Daoyu
Daoyu
@Daoyu15
@stevenemassey Only a sample is there. This cell line don’t have NGS. But it was consistent with a cell culture, and the RBD of the virus is not suitable for binding to the ACE2 found there. The ACE2 is same as Ra4331 and Ra9479.

And the RBD have a T403 that prevents infection of intestinal cells (hACE2 intestinal organoids, infection is restored upon T403R, but weaker than SARS-CoV-2) or lung cells (R.affinis primary, with supplemented hACE2. Infection is restored upon T403R).


Also that there exist no Sarbecovirus RBD with a D501.
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Sahil
Sahil
@sahilypatel
Want to do an MBA for free ?

A thread 👇

1) Leadership: Foundations of Teamwork and Leadership

School: wharton

2) Marketing: Marketing Management

School: wharton

3) Microeconomics: Microeconomics for Managers

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Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
🌺श्री गरुड़ पुराण - संक्षिप्त वर्णन🌺

हिन्दु धर्म के 18 पुराणों में से एक गरुड़ पुराण का हिन्दु धर्म में बड़ा महत्व है। गरुड़ पुराण में मृत्यु के बाद सद्गती की व्याख्या मिलती है। इस पुराण के अधिष्ठातृ देव भगवान विष्णु हैं, इसलिए ये वैष्णव पुराण है।


गरुड़ पुराण के अनुसार हमारे कर्मों का फल हमें हमारे जीवन-काल में तो मिलता ही है परंतु मृत्यु के बाद भी अच्छे बुरे कार्यों का उनके अनुसार फल मिलता है। इस कारण इस पुराण में निहित ज्ञान को प्राप्त करने के लिए घर के किसी सदस्य की मृत्यु के बाद का समय निर्धारित किया गया है...

..ताकि उस समय हम जीवन-मरण से जुड़े सभी सत्य जान सकें और मृत्यु के कारण बिछडने वाले सदस्य का दुख कम हो सके।
गरुड़ पुराण में विष्णु की भक्ति व अवतारों का विस्तार से उसी प्रकार वर्णन मिलता है जिस प्रकार भगवत पुराण में।आरम्भ में मनु से सृष्टि की उत्पत्ति,ध्रुव चरित्र की कथा मिलती है।


तदुपरांत सुर्य व चंद्र ग्रहों के मंत्र, शिव-पार्वती मंत्र,इन्द्र सम्बंधित मंत्र,सरस्वती मंत्र और नौ शक्तियों के बारे में विस्तार से बताया गया है।
इस पुराण में उन्नीस हज़ार श्लोक बताए जाते हैं और इसे दो भागों में कहा जाता है।
प्रथम भाग में विष्णुभक्ति और पूजा विधियों का उल्लेख है।

मृत्यु के उपरांत गरुड़ पुराण के श्रवण का प्रावधान है ।
पुराण के द्वितीय भाग में 'प्रेतकल्प' का विस्तार से वर्णन और नरकों में जीव के पड़ने का वृत्तांत मिलता है। मरने के बाद मनुष्य की क्या गति होती है, उसका किस प्रकार की योनियों में जन्म होता है, प्रेत योनि से मुक्ति के उपाय...
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Devdutta
Devdutta
@Devdutta96
Please please please take your cyber security seriously even if you don’t consider yourself a high risk person. Here are a few easy things to start with. 1/n

Switch from WhatsApp to Signal and enable disappearing messages. Most of us cannot stop using WhatsApp entirely but at least turn off back ups to Google Drive or iCloud. 2/n

Do not have private conversations using the messaging service on any social media platform like Facebook, Instagram or Twitter. 3/n

Switch from Gmail to ProtonMail or any other secure email service provider. 4/n


Switch from Google Docs to CryptPad or RiseUp Pad.
CYBER SECURITY , IT PRIVACY , CYBER SECURITY STEPS
anirudhsinh parmar
anirudhsinh parmar
@anirudhparmar68
Read this. U may get it for free.
Short straddle :
What is ?? Sell an atm call and put.
Instrument: mostly bnf and nf.
Now there are versions of it.
1. Best version :
Enter around 9.15-20 and exit around 3.20. No adjustment nothing.
1/n.


If u r doing it on zero leverage(meaning u have full capital of around bnf's notional contract,i.e. 40000*25 = 10lacs), then u r most likely going to make better than fd or anything like that.
Simple rules : friday,monday, tuesday do for weekly. Wednesday and thrusday next week

So, gamma moves can't impact much.
Another better version of mahesh chander kaushik is to do it for monthly expiry.
Bigger premiums and never hav to worry about 200-300 points sudden moves.
2. 2nd version : have combined stoploss of premiums and re-entry criteria. Example :

Let say 40000 straddle is at 600. Sell at 600 and hav combined sl at 650. Many algos are providing this. U can even place stoploss in % terms. Like, if sold straddle at 400 and placed 10% sl, means if 440 combined premium touch, it will be stoploss.
Let say, in morning,

U sold straddle at 600 and it sl hit at 650. Now, u placed reentry at 600 with same sl. This time may be linear decay happens and u may cover morning's loss and make some profit.
3rd version : stoplosses at each leg and trail sl to cost type.
Example , 40000 straddle at 400.
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@AchuthArora @aldothetrainer @Artofphysique_ @buzz_chronicles @rattibha Gotcha, boss!

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Laurie Voss
Laurie Voss
@seldo
The 2018 State of JavaScript survey is out. They got 20,000 responses and have some delicious, delicious data. I'mma thread in some highlights:

First up: JavaScript flavors. There's TypeScript at 47% adoption, a tad higher than npm's own survey results (which said 46%). npm's survey is coming up again soon and will ask about TypeScript in a lot more detail.


I really, *really* like SoJ's "would not use again" question, which lets people who've abandoned a tech self-identify. This is noticeable in the graph above with Flow users -- 41% of people who've used Flow say they wouldn't use it again.

Now JavaScript frameworks. It's been a full year since npm's survey, so these numbers differ from ours in the directions I expected:
React 65% (vs. 60%)
Vue 29% (vs. 24%)
Ember 5% (vs 4%, I was expecting a bigger rise)

But there's a shocker in here: Angular.


npm's survey had Angular at 40% last year and SoJ has it at either:
- 58% (if you include those who don't want to use it again)
- 24% (if you count only those who like it)
Since npm's question didn't ask if they intend to *continue* using it I think that might explain this.
TECH
Ethan \U0001f468\u200d\U0001f3a4
Ethan 👨‍🎤...
@Booligoosh
First update to https://t.co/lDdqjtKTZL since the challenge ended – Medium links!! Go add your Medium profile now 👀📝 (thanks @diannamallen for the suggestion 😁)


Just added Telegram links to https://t.co/lDdqjtKTZL too! Now you can provide a nice easy way for people to message you :)


Less than 1 hour since I started adding stuff to https://t.co/lDdqjtKTZL again, and profile pages are now responsive!!! 🥳 Check it out -> https://t.co/fVkEL4fu0L


Accounts page is now also responsive!! 📱✨


💪 I managed to make the whole site responsive in about an hour. On my roadmap I had it down as 4-5 hours!!! 🤘🤠🤘
MAKERS
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Sahil
Sahil
@sahilypatel
Want to do an MBA for free ?

A thread 👇

1) Leadership: Foundations of Teamwork and Leadership

School: wharton

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ALL , MBA , MBA SERIES
Anshul Pandey
Anshul Pandey
@Anshulspiritual
STORY OF PURANAS AND SUB PURANA (उप पुराण).

We have already gone through the series on importance & characteristics of all the eighteen Purans. We also know that originally there were hundred crore shlokas but were divided by Vyas in each Kalp and was compiled as eighteen Purans


for the common people to understand it properly and now their total is about four lakhs.

In Brahm Puran, Brahma preached this to Marichi. So this Puran of ten thousand shlokas is called Brahm Puran.

In Padma Puran, the base of the story is the appearance of Brahma on a lotus(Padm) from Vishnu' s navel.This has fifty five thousand shlokas.

Vishnu Puran is primarily based in Varah kalp. This book is on Vishnu and this book has twenty three thousand shlokas.

Vaayu Puran is written in Shwet Kalp and has twenty four thousand shlokas. Here Vayudev preaches about Dharma.

In Bhagwat Puran, which is written with the base of Gayatri mantra describes Dharma extensively. It has 18000 shlokas. It also mentions the story of Vritrasur.

In Narad Puran, which has twenty five thousand shlokas, is a sermon on Dharma by Narad based in Brihad Kalp.

Markandeya Puran has nine thousand shlokas and here it is through the birds that Dharma is described.
RELIGION
Greg Olear
Greg Olear
@gregolear
Trump is a Russian asset, a mob money launderer installed by his mob whoremasters to sow chaos and weaken the US & our allies.

EVERY SINGLE THING HE’S DONE since taking office is to achieve those purposes—that and to enrich himself personally.

[THREAD]


1/ His entire family is part of this. Melania is a fucking crook. Ivanka is a fucking crook. Junior and Gums are fucking crooks. They will all die in prison, except for Kushner, who, if he really *did* trade US intel for money, should be executed for espionage.

2/ Why do you think Trump installs uniquely corrupt & awful people to Cabinet positions? Pruitt, Zinke, Betsy, Rex/Pompeo, Wilbur "Melting Nazi Face" Ross. They are not there to serve us. They are not there to drain the swamp. They are there to BURN THE REPUBLIC DOWN.

3/ Trump steals. He takes taxpayer money—your money and mine—and lines his pockets with it. He exploits his office to make himself richer. This is all he cares about. Why are Republican citizens, you “fiscal conservatives,” not more angry about this? Riddle me THAT, #MAGA.

4/ Matt Whitaker, the human dildo and “acting AG,” was illegally installed to throw a monkey wrench in the Mueller investigation. That’s why they won’t release his financials. He’s fucking bought and sold. He’s going to prison for obstruction.
POLITICS