Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project https://t.co/HPJhwq7NgH
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https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.
Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.
The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017. https://t.co/Vh4dKy6rvI there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of
@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things
@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.
@pathogenetics @ggronvall https://t.co/2679qQiA0P
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
in fact, adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures. P681 guards the cardin-weintraub motif against cleavage in
the FCS is perfectly stable in anything that isn't VERO E6 classic or 293T-ACE2. anything that had TMPRSS2 and grown in trypsin-free media stably maintains the FCS.
in fact, the PRRARS, as opposed to other mutated cleavage sites--even the "perfect" H5CS--confers the greatest infectivity in CaLu-3 cells.
in fact, even P681R or S686G changes were less fit in CaLu-3 compared to PRRA virus--the P681R virus show either no difference or is slightly less effective compared to the P681 virus, and the S686G virus
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 Being handed to her and then read. No word that are outside of the script or are bad to the CCP’s official narrative (they are carefully trained and rehearsed before doing any webinars, meetings or interviews to U.S. scientists) are allowed in these engagements—they are allowed
@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 To engage but are not free. If they disclose anything that hurts the CCP narrative they and their families are disappeared.
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Arts are best form to Express thoughts & devotion. In All civilization Arts played very important role to express sprituality. Tibbeten Tangkhas are prime example .
List of My Paintings & Sketches
प्रलय पयोधि-जले धृतवान् असि वेदम्
विहित वहित्र-चरित्रम् अखेदम्
केशव धृत-मीन-शरीर, जय जगदीश हरे!!
छलयसि विक्रमणे बलिम् अद्भुत-वामन
केशव धृत-वामन रूप जय जगदीश हरे
नंदसि यज्ञ- विधेर् अहः श्रुति जातम्
केशव धृत-बुद्ध-शरीर जय जगदीश हरे
क्षत्रिय-रुधिर-मये जगद् -अपगत-पापम्
स्नपयसि पयसि शमित-भव-तापम्
केशव धृत-भृगुपति रूप जय जगदीश हरे
Simple and effective way 2 make Money
Idea 1:- Use pivot level like 14800 in case of nifty and sell 14800straddle monthly expiry (365+335) exit if nifty closes on daily basis below S1 or above R1
After closing below S1 if it closes above S1 next day or any day enter the same position again vice versa for R1
Idea2:- Use R1 and S1 corresponding strikes multiple
Incase of R1 15337 take 15300ce
N in case of S1 14221 use 14200pe
Sell both and hold till expiry or exit if nifty closes below S1 or above R1 around closing
If the same bounces above S1 and falls below R1 re-enfer same strikes
Use same criteria for nifty, usdinr and banknifty
(This is must)Use this margin rule for 1lot banknifty pair keep 4Lax margin
For nifty one lot keep 3Lax
For usdinr 100lots keep 4Lax
I bet you if you do this on consistent basis your ROI will be more than 70% on yearly basis.
Couldn't explain easier than this
Criticisms are most welcomed.
Seek wealth, not money or status. Wealth is having assets that earn while you sleep. Money is how we transfer time and wealth. Status is your place in the social hierarchy.
Understand that ethical wealth creation is possible. If you secretly despise wealth, it will elude you.
Ignore people playing status games. They gain status by attacking people playing wealth creation games.
You’re not going to get rich renting out your time. You must own equity - a piece of a business - to gain your financial freedom.
It's high time to expose these entities
"Hindutvawatch" - A well known fake narrative peddler website features article published by "International Christian concern" calling PM Modi as persecutor.
Let's know who these associations are
" Hindutvawatch" - A well managed vicious propaganda unit Operating from #Pakistan having presence almost everywhere in social media propagating vicious agenda of creating narrative of "Minority persecution", which is then amplified by the usual anti national elements.
When users on social media flagged fake news reporting and propagating fake agenda on twitter of this handle, someone came to cry foul and rescue - GLOBAL TIMES.
Yes, it's huge. Isn't it ? What mutual interest does China have with a Pak operated propaganda handle.
Now let's come to "International Christian concern"
So called NGO- serial fake news peddler.
Remember john chau who illegaly sneaked in Andaman and then killed by sentinels.
However , ICC termed this as
"Hindu radicals attack"
But 98.8% missed out on the best content on this platform.
Here are the best threads from 2022:
Topic: Productivity is a process of
People ask how I\u2019m so productive.— Ryan Breslow \U0001f57a (@theryanking) January 22, 2022
The secret: productivity is a process of REMOVAL.
Time is such an easy thing to waste...
Here\u2019s what you can remove from your life to save up to 60 hrs/week \U0001f447\U0001f447\U0001f447
Topic: How to multiply your
Stop trying to manage your time.— Shane | Achievement for Soloprenuers (@Shane___Martin) January 22, 2022
Start multiplying it.
Here are 5 ways to manufacture more time so you can get more of the right things done.
Topic: Life rules worth
Life rules worth breaking:— Sahil Bloom (@SahilBloom) January 22, 2022
Topic: Life advice for your
As I get closer to 40, I feel like I should pay it forward to the young bucks.— Ryan Stephens | Getting Dialed In \U0001f4c8 (@ryanstephens) January 11, 2022
Here's some advice for men in their 20's.
~ A THREAD ~
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For three years I have wanted to write an article on moral panics. I have collected anecdotes and similarities between today\u2019s moral panic and those of the past - particularly the Satanic Panic of the 80s.— Ashe Schow (@AsheSchow) September 29, 2018
This is my finished product: https://t.co/otcM1uuUDk
The 3 big things that made the 1980's/early 1990's surreal for me.
1) Satanic Panic - satanism in the day cares ahhhh!
2) "Repressed memory" syndrome
3) Facilitated Communication [FC]
All 3 led to massive abuse.
"Therapists" -and I use the term to describe these quacks loosely - would hypnotize people & convince they they were 'reliving' past memories of Mom & Dad killing babies in Satanic rituals in the basement while they were growing up.
Other 'therapists' would badger kids until they invented stories about watching alligators eat babies dropped into a lake from a hot air balloon. Kids would deny anything happened for hours until the therapist 'broke through' and 'found' the 'truth'.
FC was a movement that started with the claim severely handicapped individuals were able to 'type' legible sentences & communicate if a 'helper' guided their hands over a keyboard.
1. The United States of America is not and has never been a Democracy, we're a Federal Republic tied together by the capital Laws of the Constitution.
Everyone in the country is about to get a harsh lesson on what this really means...
2. In 1871, February 21: Congress Passes an Act to Provide a Government for the District of Columbia, also known as the Act of 1871.
With no constitutional authority to do so, Congress creates a separate form of
government for the District of Columbia.
3. A ten mile square parcel of land (see,
Acts of the Forty-first Congress,” Section 34, Session III, chapters 61 and
4. The act — passed when the country was weakened and financially depleted in
the aftermath of the Civil War — was a strategic move by foreign interests
(international bankers) who were intent upon gaining a stranglehold on the coffers and neck of America.
5. Congress cut a deal w/ the international bankers(Rothschilds of London) to incur a DEBT to said bankers. Because the bankers were not about to lend money to a floundering nation w/out serious stipulations, they devised a way to get their foot in the door of the United States.
- Have a lot of emails in the waiting list
- Become #1 on PH
- Become #1 of HN or Reddit
- Have people that saing "I'll pay"
- Have a lot of free users
The best way to know if they're actually pay for it.
The only thing that matters is your product providing a value, and you can't know this until people will pay money for it.
We can have a first signal of a product/market fit after the launch by check how many sales we've got in the first 24h. Some scale:
200+ This has a high potential.
100+: This has a potential.
50+: Some people need it.
10+: People almost don't need it.
0+: People don't need it.
This is for a single time payment product. For a subscription probably it should be less because people hate subscription.
And obviously, you should have a lot of traffic like 3-5k+.
Here is a stat for my products (macOS apps) for the first 24h after the launch:
Also, for anyone who wants to claim that Jovan Hutton Pulitzer (not his given name, but one he changed legally after the CueCat debacle) invented QR Codes: he did not. Like that other right-wing crazy man who claimed to have invented email, Jovan didn’t invent QR Codes.
He did invent the CueCat, a product that somehow raised $185 million dollars for a tethered barcode scanner for magazines and newspapers, so you could get ads from your ads. As I said, the dumbest product of all time. Good backup barcode scanners after they were hacked, however
Oh, that’s another thing. When CueCat was a thing, its systems were hacked. I guess that’s what makes Jovan a security expert. His bankrupt company from 20 years ago was hacked and widely mocked. He’s a genius.
In recent years, Jovan pivoted to being a treasure hunter who was featured on and widely mocked on Curse of Oak Island, because the man is insane.
There's a deep-rooted psychological (and biological) reason why.
And today, I'm gonna to explain all of it so you can start profiting off of this knowledge 💰💰
*Now, this thread is going to be long and in depth. Make sure to favorite the top tweet now so you can come back to it later.
So. Down to the very core of every living being (humans, dolphins, bacteria, aliens), there are only two drivers:
1. To survive
2. To reproduce
Right off the bat, we know that sex is a primary driver to human behavior.
This would also mean that each gender has its own insecurities regarding sex.
Men have a subconscious fear that their woman will leave them for someone more successful
Women have a fear that their man will leave them for someone younger & better-looking
We can conclude that men are attracted to looks, and women are attracted to status.
This is human nature down to its very core.
Crazy, blue-haired feminists will try to argue and say otherwise.
But, 50 years of feminism DOES NOT OVERRIDE millions of years of evolutionary biology.
Here's a quick visualization exercise so you can see how true all this is...