https://t.co/LjpdlHa0C7 is getting a good face-lift.
Samples now have variant tables with annotation on their impact to the gene.
There is also an IGV integration. You need IGV installed locally but the Start|Jump links will open up you BAM file in IGV.

You must have IGV open and then click the start link.
The sample's BAM will show up for viewing in IGV.
Once open, you can use the jump link to move swiftly around to each variant. This is a variant in THCAS.
You can jump to other variants in the same gene. In this case we have one non-synonymous variant in phase with a synonymous variant in THCAS.
Cannabinoid synthase genes are cute but some folks can answer those questions with HPLC.
How about flowering genes?

This sample has a disruptive in frame deletion on a late flowering gene. And it's very rare in the population.
Looks to be heterozygous....
But its never this simple in cannabis.

Let's look at what other damaging variations it has.
7 Damaging inframe deletions or insertions (indels) in Early and Late flowering genes.
When it comes to Indels, IGV can be very helpful to inspect the read frequency and if there appears to be any read mismapping with short reads. They all look real and diploid.
Here is another one in AAE1-2. This gene is involved in making the pre-cursors to cannabinoid synthesis.

It is not just a simple presence or absence of the cannabinoid synthase genes. One has to pay close attention to variants up stream in the pathway as well.
@threadreaderapp unroll

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"I really want to break into Product Management"

make products.

"If only someone would tell me how I can get a startup to notice me."

Make Products.

"I guess it's impossible and I'll never break into the industry."

MAKE PRODUCTS.

Courtesy of @edbrisson's wonderful thread on breaking into comics –
https://t.co/TgNblNSCBj – here is why the same applies to Product Management, too.


There is no better way of learning the craft of product, or proving your potential to employers, than just doing it.

You do not need anybody's permission. We don't have diplomas, nor doctorates. We can barely agree on a single standard of what a Product Manager is supposed to do.

But – there is at least one blindingly obvious industry consensus – a Product Manager makes Products.

And they don't need to be kept at the exact right temperature, given endless resource, or carefully protected in order to do this.

They find their own way.
One of the best decisions I made during a very turbulent 2020 was to leave conventional coding behind and embrace the #nocode movement. @bubble made this a reality. Although my own journey thus far is premature, I’ve learned a lot so here’s a power thread on....


‘How I created @buildcamp sales funnel landing page in under 2hours’.

Preview here 👇

https://t.co/s9P5JodSHe

Power thread here 👇

1. Started with a vanilla bubble app ensuring that all styles and UI elements were removed. Created a new page called funnel and set the page size to 960px as this allows the page to render proportionately on both web and mobile when hitting responsive breakpoints.


2. Began dropping elements onto the page to ‘find the style’. These had to be closely aligned to our @buildcamp branding so included text, buttons and groups - nothing too heavy. Played around with a few fonts, colors and gradients and thus pinned down the following style guide.


3. Started to map out sections using groups as my ‘containers’ to hold the relevant information and imagery needed to pad out the sales pitch. At this point, they were merely blocks of color #ff6600 with reduced opacity set to 5% to ease page flair.

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