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Kevin McKernan
@Kevin_McKernan 3 years, 3 months ago 353 views

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https://t.co/LjpdlHa0C7 is getting a good face-lift.
Samples now have variant tables with annotation on their impact to the gene.
There is also an IGV integration. You need IGV installed locally but the Start|Jump links will open up you BAM file in IGV.

You must have IGV open and then click the start link.
The sample's BAM will show up for viewing in IGV.
Once open, you can use the jump link to move swiftly around to each variant. This is a variant in THCAS.

You can jump to other variants in the same gene. In this case we have one non-synonymous variant in phase with a synonymous variant in THCAS.

Cannabinoid synthase genes are cute but some folks can answer those questions with HPLC.
How about flowering genes?

This sample has a disruptive in frame deletion on a late flowering gene. And it's very rare in the population.

Looks to be heterozygous....
But its never this simple in cannabis.

Let's look at what other damaging variations it has.

7 Damaging inframe deletions or insertions (indels) in Early and Late flowering genes.

When it comes to Indels, IGV can be very helpful to inspect the read frequency and if there appears to be any read mismapping with short reads. They all look real and diploid.

Here is another one in AAE1-2. This gene is involved in making the pre-cursors to cannabinoid synthesis.

It is not just a simple presence or absence of the cannabinoid synthase genes. One has to pay close attention to variants up stream in the pathway as well.

@threadreaderapp unroll

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Ok, here is a thread for you.
What happens when you have:
Netflix
Apple
Amazon
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Disney+
SonyCrackle (Reboot)
Verizon All Access (after they buy CBS/Viacom)
UniversalSkyMedia
By 2021
Answer? Internet Slows To A Craw and Dies.
Why?
Bandwidth.

Netflix's gets 35% of all internet traffic.
Now we all know Apple Coming to Netflix Corner.
We know that WarnerMedia Planning One
We Know about Disney+
Now how will the net handle 8 Streaming Platforms all at once?
Answer - IT CANT.

But Novid, the speed, the 4K the all everything?
NOOOOO BUDDY.
Even if you could do it and even if AWS ran six million clouds, The Net Will still slow to a crawl. 35%, goes to nearly 90% if any of the 8 or all of the 8 eat at netflix's numbers.

Oh, they wouldn't be running at once.
FOOL. You forget how bad things were when game of thrones season premieres came around. HBO SERVERS FALL DOWN GO BOOM!

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I’ve been flooded with Snapchat Spotlight questions after posting I’ve qualified for a payout 4 times. Here are the answers to the most common questions I’ve gotten in my DMs. These answers are my own experience and may differ from others .

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A: my experience is that usually 7-10 days after you get the qualifying message, you get a message with the amount, it will look like this. This message will include a unique access code for HyperWallet.

Q: How do I set up my HyperWallet?
A: Around three weeks after you get the payment amount you will get an email to your verified email from Snapchat Pay. It will look like this and will require you to fill out some tax forms, takes like 10 min.

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This is why we, consumers and citizens, must make the change for Facebook. Antitrust forcing a breakup of the business, and consumer revolt driving #DeleteFacebook. Zuckerberg will never conclude that what he built has become such a net negative for the world on his own.

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Netlify allows you to cancel a deploy- did you just put in a change, merge it and then realize you've made a terrible mistake? I've never done that. 😬 er...

No worries. You can cancel that deploy with the click of a button.

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You can lock a deploy in a state to get ready for a major release, so that you know exactly what you're releasing and sleep well the night before. 😴

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The BATTLE OF PAVAN KHIND (13 July 1660)

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Valour, loyalty and sacrifice!

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In this battle he became a martyr, but managed to halt the enemy forces for several hours, which secured Shivaji’s safety and victory on another front. Without this heroic last stand Shivaji’s glorious career may have been cut short.(2)

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