1. Wuhan Sewage Document Dump (Ignore the Smell)
58 Pages - Wuhan Wastewater and Stormwater Management Project
https://t.co/AyEx8UqqVK

2. Wuhan Wastewater and Stormwater Management Project (Financed by the Technical Assistance Special Fund)
1062 pages of shit
https://t.co/T2uX32FKox
3. ADB projects:
https://t.co/mV8BznlCNz
ADB documents:
https://t.co/oMwyTeNn6f
ADB Tenders:
https://t.co/nLj7TjH3jd
4. https://t.co/RRH7xAQMQI
5. Can't download this, anyone can?
https://t.co/5m34hnHyMT
6. The sewers in our city are rotten (wuhan)
https://t.co/4P2kOShEPL
7. Wuhaners are sceptical and soaked
Looking at the sea in Wuhan, why doesn't the tens of billions drainage project work?
https://t.co/S6dws4b1Ni
8. It is a shitty job but someone has to do it
(download all the shitty documents about these ADB projects)
1. Environmental Project
https://t.co/vQdm6hKEOl
2. https://t.co/caumlj2gyv
3. Project 42011-012
https://t.co/z7sKOvJbiL
4. Project 42011-013
https://t.co/z34WQ29d9V
9. Wuhan Wastewater & Stormwater Management Project | 37597-013
https://t.co/mMOGrkbJnS
Wuhan Wastewater Management Project | 34472-013
https://t.co/Ucv0rgKnIV
Wuhan Wastewater Treatment Project | 34472-012
https://t.co/fnmSfjjhHE
10. Wuhan Urban Environmental Improvement Project
Project | 42011-013
https://t.co/caumlj2gyv
Hubei Enshi Qing River Upstream Environment Rehabilitation Project Project | 47048-002
https://t.co/MTZGhNiK7K
11. Someone needs to download all this shit & it ain't me
unroll @threadreaderapp

More from Billy Bostickson 🏴👁&👁 🆓

1/ Directed Evolution and Serial passage of RNA Viruses
A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories

2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
https://t.co/BfR0B2Hwsf


3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
https://t.co/G3mIptI54H


4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
Automation

5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
https://t.co/wdKyXghRYe
Advances in the directed evolution of proteins
https://t.co/1xXTtw1i3i
In vivo continuous directed evolution
https://t.co/xKRVSjVvo4
1. Thread: RaTG13 Real or Fake?
A Norwegian Scientist @Vehuardo recently claimed RaTG13 is an "authentic virus"
https://t.co/tAXMNTwdhA
This failed to take into account the work of our DRASTIC colleagues & other published analyses.
This thread is a polite attempt to educate.

2.
https://t.co/tw606vgHwp
which says RaTG13 doesn't make sense on how it was sequenced, or how the data got so messed up in the lab as to include copy paste mistakes, bat dna, pangolin dna, rice dna, mice dna, & nobody should use it to form conclusions until it's properly vetted

2 (cont)
"sequence is incomplete & provided segment doesn't function. The identified markers suggest partial match basis & actual virus itself as described by the sequence can't functionally exist due to incoherent structures that appear to be the result of sample contamination"


3. RaTG13 integrity is further attacked here:
The Validity of critical pieces of evidence for the natural origin of SARS-CoV-2 is Dubious, and needed to be

4. and here:
Major Concerns on the Identification of Bat Coronavirus Strain RaTG13 and Quality of Related Nature
1. Mini Thread on Conflicts of Interest involving the authors of the Nature Toilet Paper:
https://t.co/VUYbsKGncx
Kristian G. Andersen
Andrew Rambaut
Ian Lipkin
Edward C. Holmes
Robert F. Garry

2. Thanks to @newboxer007 for forwarding the link to the research by an Australian in Taiwan (not on

3. K.Andersen didn't mention "competing interests"
Only Garry listed Zalgen Labs, which we will look at later.
In acknowledgements, Michael Farzan, Wellcome Trust, NIH, ERC & ARC are mentioned.
Author affiliations listed as usual.
Note the 328 Citations!
https://t.co/nmOeohM89Q


4. Kristian Andersen (1)
Andersen worked with USAMRIID & Fort Detrick scientists on research, with Robert Garry, Jens Kuhn & Sina Bavari among


5. Kristian Andersen (2)
Works at Scripps Research Institute, which WAS in serious financial trouble, haemorrhaging 20 million $ a year.
But just when the first virus cases were emerging, they received great news.
They issued a press release dated November 27, 2019:
November 3 - 9, 2019 -Wuhan Institute of Virology
International Training Course on Biosafety Laboratory Management and Technology
1. Daniel Feakes, head of UN Biological Weapons Convention implementation support agency
2. René Courcol, quality control system expert, France's BSL


List of Lecturers with short bios at
November 3 - 9, WIV Training Course:
https://t.co/Bsd9yO40JP
YUAN Zhiming Professor, Wuhan Institute of Virology, CAS (Deputy Director)
SONG Donglin, Professor, Wuhan Institute of Virology, CAS ( Deputy Director of Wuhan BSL-4 Laboratory.)

Dr. TONG Xiao Senior Engineer at WIV (Molecular Virology & (HIV) infection, mechanical & vaccine immunology) On-site Manager & Chief engineer for WIV BSL-4 Lab construction, maintenance & biosafety equipment R&D.

Zhengli Shi, Director of WIV Center for Emerging Infectious Diseases, now in charge of scientific activity in BSL3 & BSL4. Viral pathogen discovery through sequencing techniques, wildlife-borne viral pathogens, particularly bat-borne viruses since 2004.

DENG Fei, Principle Investigator in Research Group on Viruses Resources & Biotechnology in WIV, also Director of Microorganisms & Viruses Resource Center.
Graduate of ECUST, went to Great Lakes Forestry Center, Canada in 2001 & Wageningen University, NL to study baculovirus.

More from Society

Brief thread to debunk the repeated claims we hear about transmission not happening 'within school walls', infection in school children being 'a reflection of infection from the community', and 'primary school children less likely to get infected and contribute to transmission'.

I've heard a lot of scientists claim these three - including most recently the chief advisor to the CDC, where the claim that most transmission doesn't happen within the walls of schools. There is strong evidence to rebut this claim. Let's look at


Let's look at the trends of infection in different age groups in England first- as reported by the ONS. Being a random survey of infection in the community, this doesn't suffer from the biases of symptom-based testing, particularly important in children who are often asymptomatic

A few things to note:
1. The infection rates among primary & secondary school children closely follow school openings, closures & levels of attendance. E.g. We see a dip in infections following Oct half-term, followed by a rise after school reopening.


We see steep drops in both primary & secondary school groups after end of term (18th December), but these drops plateau out in primary school children, where attendance has been >20% after re-opening in January (by contrast with 2ndary schools where this is ~5%).

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