4. WTF?
Yoichi Shimatsu now brazenly accuses one of Japan's top microbiologists, Yoshihiro Kawaoka, of engineering SARS-COV-2 by inserting "HIV force multipliers", similar to his earlier work at University of Wisconsin on Influenza and HIV back in 2011.
https://t.co/ZhhZWnuKow
"HIV reverse-binding protein is essential for influenza A virus replication & promotes genome-trafficking in late-stage infection"
https://t.co/KNnU8wnj7z
"The lawless pharmaceuticals, insane vaccinators & rogue microbiologists must be stopped in their tracks or humanity will face its final solution.
High-ranking sponsors in government & major corporations also should be indicted & appropriately punished by judges"
More from Billy Bostickson 🏴👁&👁 🆓
1. Thread: RaTG13 Real or Fake?
A Norwegian Scientist @Vehuardo recently claimed RaTG13 is an "authentic virus"
https://t.co/tAXMNTwdhA
This failed to take into account the work of our DRASTIC colleagues & other published analyses.
This thread is a polite attempt to educate.
2. https://t.co/tw606vgHwp
which says RaTG13 doesn't make sense on how it was sequenced, or how the data got so messed up in the lab as to include copy paste mistakes, bat dna, pangolin dna, rice dna, mice dna, & nobody should use it to form conclusions until it's properly vetted
2 (cont)
"sequence is incomplete & provided segment doesn't function. The identified markers suggest partial match basis & actual virus itself as described by the sequence can't functionally exist due to incoherent structures that appear to be the result of sample contamination"
3. RaTG13 integrity is further attacked here:
The Validity of critical pieces of evidence for the natural origin of SARS-CoV-2 is Dubious, and needed to be
4. and here:
Major Concerns on the Identification of Bat Coronavirus Strain RaTG13 and Quality of Related Nature
A Norwegian Scientist @Vehuardo recently claimed RaTG13 is an "authentic virus"
https://t.co/tAXMNTwdhA
This failed to take into account the work of our DRASTIC colleagues & other published analyses.
This thread is a polite attempt to educate.
2. https://t.co/tw606vgHwp
which says RaTG13 doesn't make sense on how it was sequenced, or how the data got so messed up in the lab as to include copy paste mistakes, bat dna, pangolin dna, rice dna, mice dna, & nobody should use it to form conclusions until it's properly vetted
2 (cont)
"sequence is incomplete & provided segment doesn't function. The identified markers suggest partial match basis & actual virus itself as described by the sequence can't functionally exist due to incoherent structures that appear to be the result of sample contamination"
3. RaTG13 integrity is further attacked here:
The Validity of critical pieces of evidence for the natural origin of SARS-CoV-2 is Dubious, and needed to be
4. and here:
Major Concerns on the Identification of Bat Coronavirus Strain RaTG13 and Quality of Related Nature
1. WIV Research groups (archived pages) for Reference
dsRNA viruses molecular biology
https://t.co/lwrQoo6ygG
2. Emerging Viruses Group
https://t.co/ND56gVGOAn
3. Structural Biology of Viral Genome Replication
https://t.co/wUqc0YIP3X
4. Molecular Biology of hepatitis Viruses & Gene Therapy
https://t.co/snBbxkhNsC
5. Insect Virus Genetic Engineering Lab
https://t.co/S68mVOA2Ob
dsRNA viruses molecular biology
https://t.co/lwrQoo6ygG
2. Emerging Viruses Group
https://t.co/ND56gVGOAn
3. Structural Biology of Viral Genome Replication
https://t.co/wUqc0YIP3X
4. Molecular Biology of hepatitis Viruses & Gene Therapy
https://t.co/snBbxkhNsC
5. Insect Virus Genetic Engineering Lab
https://t.co/S68mVOA2Ob
Keep dwelling on this:
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.
The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains
(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK
In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa
Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.
The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains
(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK
In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa
Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs
1/10 What's all this then?
Biotech or biohazard?
A proposed federal lab in Butner would study the world's deadliest diseases
https://t.co/krnmueAd80
is it being built there or elsewhere, at Kansas University?
2/10 Shades of Plum Island?
New Proposal: https://t.co/Lv3FgBgyas
GAO reports on security and safety lapses at Plum Island Animal Disease Research Center
Plum Island 1: https://t.co/pXSHgg1KKN
Plum Island 2:
https://t.co/O9oS0WXYOz
3/10 National Bio and Agro-Defense Facility
By memo dated December 4, 2008, the Department of Homeland Security named the Kansas site as the preferred location for the NBAF. On January 16, 2009, the record of decision was published in the Federal
4/10 Lucky ole Kansas!
The $1.25 billion facility is a biosafety level-4 laboratory and will replace the aging Plum Island Animal Disease Center.
NBAF is expected to be operational by 2022-2023.
Manhattan, Kansas, was selected as the location for NBAF.
https://t.co/kiokkFsT6v
5/10 The facility continues forward - Apr 19, 2020
https://t.co/JlstInKIRS
https://t.co/DtbLD9Tvxb
The 500,000 square foot building will have a $3.5 billion impact on the Kansas economy and many lab
Biotech or biohazard?
A proposed federal lab in Butner would study the world's deadliest diseases
https://t.co/krnmueAd80
is it being built there or elsewhere, at Kansas University?
2/10 Shades of Plum Island?
New Proposal: https://t.co/Lv3FgBgyas
GAO reports on security and safety lapses at Plum Island Animal Disease Research Center
Plum Island 1: https://t.co/pXSHgg1KKN
Plum Island 2:
https://t.co/O9oS0WXYOz
3/10 National Bio and Agro-Defense Facility
By memo dated December 4, 2008, the Department of Homeland Security named the Kansas site as the preferred location for the NBAF. On January 16, 2009, the record of decision was published in the Federal
4/10 Lucky ole Kansas!
The $1.25 billion facility is a biosafety level-4 laboratory and will replace the aging Plum Island Animal Disease Center.
NBAF is expected to be operational by 2022-2023.
Manhattan, Kansas, was selected as the location for NBAF.
https://t.co/kiokkFsT6v
5/10 The facility continues forward - Apr 19, 2020
https://t.co/JlstInKIRS
https://t.co/DtbLD9Tvxb
The 500,000 square foot building will have a $3.5 billion impact on the Kansas economy and many lab