
More from Billy Bostickson 🏴👁&👁 🆓
1/ Directed Evolution and Serial passage of RNA Viruses
A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories
2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
https://t.co/BfR0B2Hwsf
3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
https://t.co/G3mIptI54H
4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
Automation
5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
https://t.co/wdKyXghRYe
Advances in the directed evolution of proteins
https://t.co/1xXTtw1i3i
In vivo continuous directed evolution
https://t.co/xKRVSjVvo4
A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories
2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
https://t.co/BfR0B2Hwsf

3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
https://t.co/G3mIptI54H

4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
Automation
5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
https://t.co/wdKyXghRYe
Advances in the directed evolution of proteins
https://t.co/1xXTtw1i3i
In vivo continuous directed evolution
https://t.co/xKRVSjVvo4

1. Project 1742 (EcoHealth/DTRA)
Risks of bat-borne zoonotic diseases in Western Asia
Duration: 24/10/2018-23 /10/2019
Funding: $71,500
@dgaytandzhieva
https://t.co/680CdD8uug
2. Bat Virus Database
Access to the database is limited only to those scientists participating in our ‘Bats and Coronaviruses’ project
Our intention is to eventually open up this database to the larger scientific community
https://t.co/mPn7b9HM48
3. EcoHealth Alliance & DTRA Asking for Trouble
One Health research project focused on characterizing bat diversity, bat coronavirus diversity and the risk of bat-borne zoonotic disease emergence in the region.
https://t.co/u6aUeWBGEN
4. Phelps, Olival, Epstein, Karesh - EcoHealth/DTRA
5, Methods and Expected Outcomes
(Unexpected Outcome = New Coronavirus Pandemic)
Risks of bat-borne zoonotic diseases in Western Asia
Duration: 24/10/2018-23 /10/2019
Funding: $71,500
@dgaytandzhieva
https://t.co/680CdD8uug

2. Bat Virus Database
Access to the database is limited only to those scientists participating in our ‘Bats and Coronaviruses’ project
Our intention is to eventually open up this database to the larger scientific community
https://t.co/mPn7b9HM48

3. EcoHealth Alliance & DTRA Asking for Trouble
One Health research project focused on characterizing bat diversity, bat coronavirus diversity and the risk of bat-borne zoonotic disease emergence in the region.
https://t.co/u6aUeWBGEN

4. Phelps, Olival, Epstein, Karesh - EcoHealth/DTRA

5, Methods and Expected Outcomes
(Unexpected Outcome = New Coronavirus Pandemic)

1. Omicron Thread - A Compilation of Opinions
"finally, the time has slip and slid its slimy course through to us - and we're the vicious bastards of the new generation. we do not relent, and we should not be held to account for our ancestral faults"
https://t.co/mhJO6yOQRE
2. The Letter Chosen by the WHO
"It's amazing that the Xi variant seems to have somehow managed to evade detection..."
via @readomain
Why 'Omicron', Not 'Nu' or 'Xi'?
WHO Says Two Letters in Greek Alphabet Jumped to 'Avoid Stigma'
https://t.co/xnGR9XK2tF
3. The Omicron variant has been found in nearly every province of South Africa, as well as Botswana, Belgium, Israel, UK and Hong Kong.
This month, Israel held an exercise, The “Omega Exercise”, meant to prepare for a hypothetical, new COVID-19
4. Omicron Infographic
(appeared in Germany and Italy now)
https://t.co/oNFYBH1F1o
5. Overview of Omicron (B.1.1.529) from @nicd_sa
https://t.co/HN9csJqK0z
"finally, the time has slip and slid its slimy course through to us - and we're the vicious bastards of the new generation. we do not relent, and we should not be held to account for our ancestral faults"
https://t.co/mhJO6yOQRE

Here is how B.1.1.529 (#Omicron #B11529) compares to Alpha, Beta, Gamma, Delta variants.
— nference (@_nference) November 27, 2021
Omicron has highest novel Spike mutations including striking cluster on the "crown" suggesting significant selection pressure & antigenic distinction from prior strains
(Credits: nference) pic.twitter.com/4oZQbjhbG8
2. The Letter Chosen by the WHO
"It's amazing that the Xi variant seems to have somehow managed to evade detection..."
via @readomain
Why 'Omicron', Not 'Nu' or 'Xi'?
WHO Says Two Letters in Greek Alphabet Jumped to 'Avoid Stigma'
https://t.co/xnGR9XK2tF

3. The Omicron variant has been found in nearly every province of South Africa, as well as Botswana, Belgium, Israel, UK and Hong Kong.
This month, Israel held an exercise, The “Omega Exercise”, meant to prepare for a hypothetical, new COVID-19
4. Omicron Infographic
(appeared in Germany and Italy now)
https://t.co/oNFYBH1F1o

5. Overview of Omicron (B.1.1.529) from @nicd_sa
https://t.co/HN9csJqK0z

Prof @Tuliodna is giving an insightful overview of the #NewVariant #B11529 pic.twitter.com/VnFBrj2pOT
— NICD (@nicd_sa) November 25, 2021
Meet Yang Ruifu, CCP's biological weapons expert https://t.co/JjB9TLEO95 via @Gnews202064
Interesting expose of China's top bioweapons expert who oversaw fake pangolin research
Paper 1: https://t.co/TrXESKLYmJ
Paper 2:
https://t.co/9LSJTNCn3l
Pangolin
https://t.co/2FUAzWyOcv
Ruifu, obviously obsessed with PLAGUE,
https://t.co/7A9NiRlNoz
is a friend of Oliver Pybus (one of the Holmes, Andersen, Rambaut, Lipkin Gang)
Oliver Pybus helped Ruifu pump out CCP Propaganda
see next tweet
Pybus and Ruifu
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
https://t.co/fl8yh0PSI8
and
China's control measures may have prevented 700,000 COVID-19
Email for academic queries:
[email protected] (H.T.);
[email protected] (C.D.); [email protected] (O.G.P.);
[email protected] (B.T.G.)
[email protected] (R.Y.)
Pangoscam
Interesting expose of China's top bioweapons expert who oversaw fake pangolin research
Paper 1: https://t.co/TrXESKLYmJ
Paper 2:
https://t.co/9LSJTNCn3l
Pangolin
https://t.co/2FUAzWyOcv

Ruifu, obviously obsessed with PLAGUE,
https://t.co/7A9NiRlNoz
is a friend of Oliver Pybus (one of the Holmes, Andersen, Rambaut, Lipkin Gang)
Oliver Pybus helped Ruifu pump out CCP Propaganda
see next tweet
Pybus and Ruifu
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
https://t.co/fl8yh0PSI8
and
China's control measures may have prevented 700,000 COVID-19
Email for academic queries:
[email protected] (H.T.);
[email protected] (C.D.); [email protected] (O.G.P.);
[email protected] (B.T.G.)
[email protected] (R.Y.)

Pangoscam
