Let Uncle Ralph educate you
Synthetic Viral Genomics:
Risks and Benefits for Science and Society
These enzymes can be used to create unique interconnecting junctions, which can be subsequently removed from final assembly product allowing seamless reconstruction of an exact sequence
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
This powerful technique provides bioterrorists with a “scapegoat” option; leaving a sequence signature that misdirects efforts at tracking the true originators of the crime. Even better, the approach could be used to build mistrust &/or precipitate open warfare
Hence Ecohealth DARPA/DTRA spooks & virus thieves collaboration with Baric (UNC) Lipkin (Mailman) NIchols (Atlanta CDC) and USAMRIID (Bavari, Totura et al) & Jonathan Epstein's palpable concern about dual use references in the @USRightToKnow FOIA emails
Delving into the cold and calculating mind of a twisted genius?
Bring Uncle Ralph and his transgenic mice in for questioning!
1. Synthetic Genomics: Options for Governance (2008) https://t.co/A32jEfL1TQ
2. Sequence Screening - Robert Jones (2005)
3. Synthetic Biology as a Field of Dual-Use Bioethical Concern - Alexander Kelle
4. Sanghvi Y. A Roadmap to the Assembly of Synthetic DNA from Raw Materials.
5. Collett MS. Impact of Synthetic Genomics on the Threat of Bioterrorism with Viral Agents.
6. Fleming DO. Risk Assessment of Synthetic Genomics: A Biosafety & Biosecurity Perspective.
7. Risk Governance of Synthetic Biology
8. US Competitiveness in Synthetic Biology
9. Ensuring security of synthetic biology
10. Synthetic biology: emerging research field in China
What rough beast? Synthetic biology, uncertainty,& the future of biosecurity (2016)
Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus (2003)
Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 (2008)
In 2016, Shi and her team at the WIV, in conjunction with the New York-based EcoHealth Alliance, constructed a full-length clone of a bat coronavirus called SL-CoV WIV1. They assembled it in discrete segments.
“Thus, several options exist to remove the desired insert DNA with a single restriction digestion.”
This shows that researchers at the WIV have the ability to genetically engineer viruses and remove the signatures of the genetic engineering.
They showed how they can insert new spikes into viruses. The researchers state:
“Then any spike could be substituted into the genome of SARSr-CoV WIV1 through this strategy.”
More from Billy Bostickson 🏴👁&👁 🆓
@NBA @StephenKissler @yhgrad 1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice
1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 30, 2021
"we generated a mouse model expressing hACE2 by using CRISPR/Cas9 knockin technology. In comparison with wild-type C57BL/6 mice, both young & aged hACE2 mice sustained high viral loads... pic.twitter.com/j94XtSkscj
@NBA @StephenKissler @yhgrad 2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2
1. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2!— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 2, 2021
Human\u2013viral molecular mimicryhttps://t.co/irfH0Zgrve
Molecular Mimicryhttps://t.co/yLQoUtfS6s https://t.co/lsCv2iMEQz
@NBA @StephenKissler @yhgrad B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.
@NBA @StephenKissler @yhgrad We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the ... Thus, this mouse-adapted strain and associated challenge model should be ... (B) SARS-CoV-2 genomic RNA loads in mouse lung homogenates at P0 to P6.
A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories
2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
Advances in the directed evolution of proteins
In vivo continuous directed evolution
Liu Xin's exclusive interview with China's 'Batwoman' https://t.co/Tsno7CRVys via @YouTube
1. Shi Claims ther never have been any lab leaks at P4 or the 2 P3s
2. Shi claims her lab records are transparent & available for inspection
3. Shi says all experimental work has been published
4. Shi claims her database is open for inspection!
@interne41914499 compares the influenza diagnosis & treatment plan by China NHC on 13 Nov 2019 with the 2018 plan.
Medical staff training manuals for influenza were also issued & included similar changes between 2019 &
Taking this research as a starting point, I will look at the people responsible for drafting the influenza plan in 2019
1. The National Health Commission (NHC)
Guójiā Wèishēng Jiànkāng
2. Cabinet-level executive department of State Council which is responsible for formulating health policies in Mainland China.
Formed on 19 March 2018.
Its predecessor was the National Health and Family Planning Commission.
Here is the leadership list:
Boss is Ma Xiaowei
Current Minister in charge of the Commission and Party Branch Secretary.
Ma Xiaowei sucks Xi's dick for
In memo discussed here:
Ma demanded officials unite around Xi because social stability was priority in the lead up to the upcoming March CCP State Meetings:
Avoid causing panic among the masses!
Seems that Eddie was playing the "innocent card"
@TheSeeker268 @franciscodeasis @ydeigin @AntGDuarte @edwardcholmes @K_G_Andersen
Edward Holmes on being asked why they didn't release the SARS-CoV-2 genome when they got it: "...essentially there were instructions that there's no publicizing the outbreak, so we kept it to ourselves, we told the key people in China..but we were told not to release the data..." pic.twitter.com/mAGUUrsiet— The Seeker (@TheSeeker268) April 9, 2021
2. Was the motive of https://t.co/nu7hNVhiwP’s rush release due to competition to get the first releaser credit for the sars-cov-2 genome?
3. @GISAID claims 'the access credentials of some of the persons listed as authors of Wuhan-hu-1 were among the access credentials used on January 10, 2020 to access the first genomes available on GISAID'
4. @GISAID formally wrote to the authors who acknowledged Virological,org’s post as the world's first release of the SARS-COV-2 genome, and penalised the authors of MN908947
5. but @GISAID didn't identify who exactly from Fudan University or University of Sydney ACCESSED and KNEW about IVDC's 'initial' submission of IVDC-01/05 on GISAID on 10th Jan, 2020, via login records of their GISAID account credentials.
More from Science
Thread for today. /0
It is fashionable for people to create sympathy to the unseen / unknown organisms. The poor things that can't stand up and fight for their own existence.
It makes for a brilliant narrative. It melts your hearts. And you get to play saviour of the oppressed by outraging. /1
But, for most part, such things are also false.
Amazon are the lungs of the world. Never mind that most of the world's oxygen production happens in open oceans. But these are harmless ones. Of course, we all love to maintain forests.
However, some things are sheer scare mongering.
For instance - take this: coral reefs will be permanently gone if mean / median temperatures raise by 1°C ..
But, really - how? Then you get a lecture on how CO2 will affect calcification.
Dig a bit deeper from another angle+
+ and you find that the earth was much much warmer during the age of Dinosaurs. So much so, that we had cold blooded lizards growing to sizes of buildings. Surely, there must have been tons of CO2 then!
And the ocean temps were quite high too.
If you are into cryptography or reverse engineering, you should love this.
DNA consists of four different 'bases', A, C, G and T. These bases have specific meaning within our biology. Specifically, within the 'coding part' of a gene, a triplet of bases encodes for an amino acid
Most DNA is stored redundantly, in two connected strands. Wherever there is an A on one strand, you'll find a T on the other one. And similarly for C and G:
T G T C A G T
A C A G T C A
(note how the other strand is upside down - this matters!)
If you take all the DNA of an organism (both strands), you will find equal numbers of A's and T's, as well as equal numbers of C's and G's. This is true by definition.
This is called Chargaff's 1st parity rule.
Strangely enough, this rule also holds per strand! So even if you take away the redundancy, there are 99% equal numbers of A/T and C/G * on each strand *. And we don't really know why.
This is called Chargaff's 2nd parity rule.
Like any healthcare professional, I have been quizzical over certain decisions, but I doubt that Whitty has been the problem. We are led by science, but politics delays the response. We should have locked down earlier, opened later, and been more restrictive.
The #eatouttohelpout campaign was a political exercise that led to disaster. The Tier system was recognised early as a bad idea. The push from scientists and medical teams has always been to lock down earlier, restrict more. This is not nice, but nescessary.
It has been Johnson and Co who have delayed everything, tried to have their cake and eat it, sold PPI contracts and track and trace to cronies and friends. It has been certain pundits and commentators that have denied the outbreaks and slowed responses and sowed doubt.
The government will look for a scapegoat, and now they don't have the EU they will blame the 'experts' as per the brexit scheme, no blame can reflect on themselves. Note how this works, if they can't blame Whitty they will blame immigrants and mental health.
Why is it that you can boil a frog in water without it jumping out if you gradually increase the temperature?
The problem for the frog is that it does not have the internal models to realize that there is a change in the water temperature. A cold-blooded creature like the frog has its temperature regulated by the external environment.
To recognize change, an agent must have an internal model of reality that is able to recognize this change. Unfortunately, a majority of the population do not have good models of human general intelligence.
In fact, even the simplistic dual-process model of system 1 and system 2 is not very well known.
Variants always emerge, & are not good or bad, but expected. The challenge is figuring out which variants are bad, and that can't be done with sequence alone.
Feels like the next thing we're going to need is a ranking system for how concerning "variants of concern\u201d actually are.— Kai Kupferschmidt (@kakape) January 15, 2021
A lot of constellations of mutations are concerning, but people are lumping together variants with vastly different levels of evidence that we need to worry.
You can't just look at a sequence and say, "Aha! A mutation in spike. This must be more transmissible or can evade antibody neutralization." Sure, we can use computational models to try and predict the functional consequence of a given mutation, but models are often wrong.
The virus acquires mutations randomly every time it replicates. Many mutations don't change the virus at all. Others may change it in a way that have no consequences for human transmission or disease. But you can't tell just looking at sequence alone.
In order to determine the functional impact of a mutation, you need to actually do experiments. You can look at some effects in cell culture, but to address questions relating to transmission or disease, you have to use animal models.
The reason people were concerned initially about B.1.1.7 is because of epidemiological evidence showing that it rapidly became dominant in one area. More rapidly that could be explained unless it had some kind of advantage that allowed it to outcompete other circulating variants.
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There's a deep-rooted psychological (and biological) reason why.
And today, I'm gonna to explain all of it so you can start profiting off of this knowledge 💰💰
*Now, this thread is going to be long and in depth. Make sure to favorite the top tweet now so you can come back to it later.
So. Down to the very core of every living being (humans, dolphins, bacteria, aliens), there are only two drivers:
1. To survive
2. To reproduce
Right off the bat, we know that sex is a primary driver to human behavior.
This would also mean that each gender has its own insecurities regarding sex.
Men have a subconscious fear that their woman will leave them for someone more successful
Women have a fear that their man will leave them for someone younger & better-looking
We can conclude that men are attracted to looks, and women are attracted to status.
This is human nature down to its very core.
Crazy, blue-haired feminists will try to argue and say otherwise.
But, 50 years of feminism DOES NOT OVERRIDE millions of years of evolutionary biology.
Here's a quick visualization exercise so you can see how true all this is...
Reliable source tells me Withdrawal Deal text contains
- No unilateral exit from backstop
- Large annexe on level playing field
.... after reporting this on @SkyNews I get a call from a different senior source that this is “spot on” and further that the Attorney General Geoffrey Cox has not pulled his punches in confirming in legal advice there is “no unilateral exit” from backstop ...
... furthermore I’m told (and this chimes with excellent Times splash this morning re Weyand) that the future partnership then takes its starting point as the activated backstop - ie UK-wide customs union-style arrangement and level playing field.
Understand that a ministerial briefing on the draft Withdrawal Agreement meant to drum up support from some trade organisations due tonight has now been postponed
In 1989, two members of the group, Morten Harket and Magne Furuholmen were in Switzerland with environmentalist Frederic Hauge, when they came across a hobby-converted Fiat Panda. Stated range: 45 km. They snapped it up and imported it to Norway.
Photo: In Switzerland (Bellona)
On arrival in Norway, the regs didn't accommodate the registration of electric cars so it couldn't legally be driven on the road. Since it had a propane-fuelled heater, just like a motorhome, they registered it as a motorhome.
Moreover, in contrast to petrol cars, diesel cars paid registration fees based on how far they were driven, and our heroes thought this should also apply to electric cars. So the two-seater Swiss-converted Fiat Panda was registered as a diesel motorhome in Norway.
They managed (somehow) to avoid paying the one-off registration fee in 1990, and ever since then electric cars have been exempted this now-substantial fee.
Last year, we upgraded tweet length to 280 and guess what? Use of 'please' is up 54% and 'thank you' is up 22%. I love that! Also, people are asking more questions and having more conversations. All this, and the majority of tweets are still under 140. It worked.
His parents, my Omi and Opi, always considered themselves more German than Jewish, so they didn’t think they’d get swept up in the madness.
Opi was taken to city hall with other Jews and lined up against a wall. He thought he was going to be shot.
He always remembered when they got to Dachau there were pajamas with Jewish stars waiting on their cots. Obviously not a spontaneous thing.
He was very lucky. His family got him a fake Uruguayan visa and he was out of Dachau six weeks later. “Before Christmas,” my dad recalls.