Interesting!
— Florin (@Florin_Uncovers) February 20, 2022
NAHL Laos was built by USAID PREDICT to work with Metabiota. Daszak says Sep 2018 they were still not allowed to collect samples for Shi at WIV.
Doesn't mean EcoHealth didn't get them before/after directly/indirectly. We know the Navy sampled bats there in 2017.\U0001f642 pic.twitter.com/mVp6dytD6X
EXCLUSIVE: Hunter Biden DID help secure millions in funding for US contractor in Ukraine specializing in deadly pathogen research https://t.co/fFBX7IFcKh
— Daily Mail US (@DailyMail) March 25, 2022
PREDICT: Ecohealth; Metabiota; WIV; found every virus under the Sun in the bat caves in S.China in 2014, but "NONE" at any wet market where humans gather! What was their research really all about?? \U0001f447https://t.co/Qun3IHsdUV pic.twitter.com/7Hb881BxyH
— Keith Evans (@KeithEv84928885) April 29, 2022
24. DTRA Network for Collection of Viruses
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 9, 2021
7. DTRA - Metabiota - One Health - Ecohealth
Bat Research Networks and Viral Surveillance: Gaps and Opportunities in Western Asia pic.twitter.com/SOqSSXF3pa
23. David Mustra can be seen at the 2:34 mark in the video, note: "Metabiota"
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) April 14, 2022
Note: Martha Stokes from DTRA also appears in the video, who will be familiar to some of you from Cambodian Bat Research and BOHRN (with Dr. Jonathan Epstein (EHA).https://t.co/SSawjbdTcC pic.twitter.com/CG8sfoPz61
Metabiota is getting funding from all the wrong placeshttps://t.co/BVs01rQCBr pic.twitter.com/3nuyz0FVaT
— DIY (@mindnotforrent) March 26, 2022
https://t.co/QpHGiNN8Fg
— Daoyu (@Daoyu15) May 31, 2022
In the CAS implementation of DEFUSE, S.E. Asia is clearly in the scope. https://t.co/4th2qCSr3X
Specifically, \u201csamples from surrounding areas of my country and the Belt and Road regions\u201d was set into the scope for the umbrella project where the two CAS
Metabiota/USAID planned visit in Laos in Oct 18
— Gilles Demaneuf (@gdemaneuf) January 11, 2022
p. 39/41 of part 3.@garyruskin pic.twitter.com/NHwK5XXryg
A lot of the South-East Asia USAID/EHA/Metabiota work is coordinated via Bangkok, where Sudarat has been a key trusted partner for many years.
— Gilles Demaneuf (@gdemaneuf) January 11, 2022
The project umbrellas include PREDICT, the Global Virome Project, OneHealth, SEAOHUN and other initiatives. pic.twitter.com/vog2oLd5Vl
#Metabiota were aligned to Government contracts, which in 2014, far out-weighed Commercial contracts. pic.twitter.com/s1oGQlCaNC
— Fools Multiply (@FoolsMultiply) April 6, 2022
How much was passed to #Metabiota ? #InstitutPasteur #UCDavis #Ecohealth #Predict pic.twitter.com/Rrxm9b6gfX
— Fools Multiply (@FoolsMultiply) April 6, 2022
An 2010 RacCS203-associated RdRp. Part of the PREDICT consortium samples. This is proof that IP/PREDICT are in possession of close SARS-CoV-2 relatives in their databanks at least since 2010. Also why this is published so late?
All the diversity in the Shitou cave have been fully sampled, the ~9 Spike proteins and the ~5 representative (within 97% similarity) strains fully tested as by year 2017. A year before DEFUSE. The word \u201c6 asian countries\u201d appeared in the document for 2 times, where the data from
— Daoyu (@Daoyu15) May 31, 2022
In addition, the sequence acquisition step stated that consensus between strains are to be used\u2014in stead of a more highly accurate deep sequencing of (newly acquired) samples, with the workflow explicitly designed to remove sequencing errors by averaging assembled sequences in
— Daoyu (@Daoyu15) June 4, 2022
Stead of sequencing each sample multiple times at a higher depth (which is how high accuracy error-free sequencing is performed by standard). This indicate that the scope of DEFUSE included \u201cQS strains\u201d where only a consensus sequence was available and of which re-sequencing
— Daoyu (@Daoyu15) June 4, 2022
is not possible, a scope that clearly included 1: non-newly-acquired samples. 2: samples that were already fully depleted (and where only sequencing data exists) at the time of the beginning of the program. 3:QS strains where deep sequencing is not conducted and only an assembled
— Daoyu (@Daoyu15) June 4, 2022
sequence was available at the time of the beginning of the program.
— Daoyu (@Daoyu15) June 4, 2022
This represents a great deal of flexibility toward sampling protocol, condition of sample and original experimental protocol, indicating that the scope of the QS recovery operation is intended to span the entire
EHA database\u2014where the majority of the strains within contained only an assembly sequence but neither samples to deep resequenced nor sequencing data that may be used to make a non-multi-strain consensus. Only when the sampling is being done by multiple institutions with a wide
— Daoyu (@Daoyu15) June 4, 2022
variety of protocol and sample condition at sequence release, will you need such a multi-strain consensus protocol, that even explicitly stated that \u201cwe will review past deep sequencing data\u201d, that is clearly designed for generalizability in stead of precision.
— Daoyu (@Daoyu15) June 4, 2022
The protocol for sequence acquisition and recovery is clearly intended for databases that contained a lot of different (un-controllable) experimental procedures in stead of those that contained only a single (specify-able from the project) experimental procedure.
— Daoyu (@Daoyu15) June 4, 2022
Now check this, #NIH's list of labs in low-resource countries. You not gonna believe this, check locations and then compare reported outbreaks past 2 decades \U0001f914\U0001f9d0#BioLabs #USA #NIH #CDC #USAID #DoD #WHO #PasteurInstitute #WorldBank #UNESCO #AFD #JICAhttps://t.co/fZ1Xktf7a1 https://t.co/42hwuNVKBP pic.twitter.com/Zoi4dcHMUr
— Dieda (@DiedaExploring) June 14, 2022
https://t.co/o4QhOeAGDk
— Daoyu (@Daoyu15) May 20, 2022
IP collaboration with WIV\u2026\u2026(IP france. What was the \u201cSARS-like coronaviruses(unpublished data)\u201d? (Contained Yunnan bat samples)
https://t.co/0CMXHB2sKu
— Daoyu (@Daoyu15) May 1, 2022
Also BANAL caves were first sampled in 2017. https://t.co/9NFcvmc6gmhttps://t.co/G5nPPPen8v
None of the reads from even the batflies, part from what that have been aligned to a single Orthoreovirus, have been released. https://t.co/BJx4j5Jq3L https://t.co/aOHh4ZSkfC
"Bats for Peace"? Funded by DTRA...https://t.co/pmFrpgkQEC
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) March 15, 2022
Links to archived documents of interesthttps://t.co/1X86x6sIl7 pic.twitter.com/9A9owXV3tj
EHA is known to use funding intended for one project for other projects—including “unfunded projects”.
This connection between PREDICT, PREDICT-2, IP (Laos, Cambodge, France) and the EHA database (used for DEFUSE), as well as the fact that the EHA splits funding amongst programs internally, is why it takes so long for them to release any data at all, and
The initial expedition with Institut Pasteur du Laos was funded by US Naval MRC and DTRA in support of the US DoD's GEIS. Yes there is a story there, and what happened to the samples from that.https://t.co/2wL5EpSdlV
— Dog's Breakfast (@breakfast_dogs) February 17, 2022
What was the “partners” of IP laos?
https://t.co/kZQhJXH01e
https://t.co/fMNUoYNnoV
https://t.co/hcVNESCpes
https://t.co/GruWYr5F89
Still seems odd Navy is overseer. Especially since a different DOD agency DTRA is EcoH's main funder at $37.86 million. Navy heavy into bat research, funding a Laos bat trip "U.S. Naval Medical Research Center-Asia (NMRC-A)" for "DoD-GEIS\u201d. DoD sure does like bat viruses & EcoH. pic.twitter.com/I0bAypew8H
— sheila (@capitolsheila) November 4, 2021
This 2017 US Navy-funded study of Laos bats turned up lots of stuff the French group isn\u2019t allowed to publish, we hear. French go back in 2020 & now tell world \u201cHey guys, bat cave in Laos has really similar stuff to SARS2.\u201d
— sheila (@capitolsheila) December 10, 2021
Laos bat viruses? DOD?
Secrets in a pandemic? Why? pic.twitter.com/1DXdlVykla
And that\u2019s not all\u2014the EcoHealth grant document also mentions plans for more sampling in SE Asia (including Laos) from Year 3 onwards, as part of their Specific Aims. pic.twitter.com/PUdEE1KZQA
— The Seeker (@TheSeeker268) January 18, 2022
Peter Daszak asked for US-funded virus data to be withheld, FOIAed by @USRightToKnow
— Alina Chan (@Ayjchan) January 11, 2022
"It's extremely important that we don't have these sequences as part of our PREDICT release.. Having them as part of PREDICT will being very unwelcome attention"https://t.co/yooVvKtkaJ pic.twitter.com/IVFJIZ9y1I
Ecohealth also “trained” (established moles inside) “students from the National university of Laos” for their own sampling purposes. PREDICT 2014.
A collaboration was later established between the EHA and the National University of Laos, sacking 69 more bats at the end of 2019 in the PREDICT-2 program.
Also notable is that Shi Zhengli was awarded nearly $1.5 million in January 2018 \u2013 a little more than what Peter Daszak had asked DARPA for WIV\u2019s part of the work (~$1.2 million).https://t.co/qYTWnm1ZVqhttps://t.co/xSpILJeY1Y pic.twitter.com/lHPXay7nW1
— The Seeker (@TheSeeker268) March 13, 2022
\u25cf Sometime in 2018: A chart shows USAID provided $270,969 to Global Virome Project (GVP) pic.twitter.com/LWYhl61xJK
— The Seeker (@TheSeeker268) February 23, 2022
From: Frieman, Matthew
— Jess (@uacjess) May 7, 2022
February 28, 2019
"Inglesby is pushing on the fact that its not reported what it was that was discussed/changed/agreed to during this new approval process that allowed the Yoshi and Fouchier experiments/grants to be approved again." @T_Inglesby pic.twitter.com/PXepYik9xb
2/28/2019
— Jess (@uacjess) May 7, 2022
Ralph Baric
"Thanks for passing this on. It would be of value to hear about the additional safeguards, although I am overall uncertain as to what NIH is doing with SARS and MERS grants, beyond the "general" statement "no PC30 safety concerns". https://t.co/zSh3OHaa9X pic.twitter.com/g1aFRR2wUE
A spike in readership of many articles related to CoVs and FCS happened in mid-late 2019. https://t.co/v1HEss37Ai
Pei-Yong Shi, who works at the University of Texas Medical Branch\u2019s biolab, has been at the DOD/NIH/CDC-funded Texas lab for over 5 yrs.
— sheila (@capitolsheila) June 18, 2022
In addition to visiting the WIV Oct. 19-20, 2019, he also was at China\u2019s newest BSL-4 lab, just as it was getting final certification to open. pic.twitter.com/mxgP0lbL8z
There is plenty there that you may find interesting, especially related to Kunming ABSL-4, the 'old' new BSL-4 in China, compared to the WIV which was the \u2018only **publicly** known P4 lab\u2019 (all wordings from FOI'd emails). pic.twitter.com/KfqMAbPurt
— Gilles Demaneuf (@gdemaneuf) June 19, 2022
More from Daoyu
@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the
chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project
starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".
P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!
https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the
chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project
starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".
P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!
https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
@JackDempsey2_2 @pathogenetics @ggronvall
@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017. https://t.co/Vh4dKy6rvI there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of
@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things
@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.
@pathogenetics @ggronvall https://t.co/2679qQiA0P
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017. https://t.co/Vh4dKy6rvI there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of
@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things
@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.
@pathogenetics @ggronvall https://t.co/2679qQiA0P
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
@franciscodeasis https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.
https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV
https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.
Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.
The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV
https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.
Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.
The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
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So it's now October 10, 2018 and....Rod Rosenstein is STILL not fired.
He's STILL in charge of the Mueller investigation.
He's STILL refusing to hand over the McCabe memos.
He's STILL holding up the declassification of the #SpyGate documents & their release to the public.
I love a good cover story.......
The guy had a face-to-face with El Grande Trumpo himself on Air Force One just 2 days ago. Inside just about the most secure SCIF in the world.
And Trump came out of AF1 and gave ol' Rod a big thumbs up!
And so we're right back to 'that dirty rat Rosenstein!' 2 days later.
At this point it's clear some members of Congress are either in on this and helping the cover story or they haven't got a clue and are out in the cold.
Note the conflicting stories about 'Rosenstein cancelled meeting with Congress on Oct 11!"
First, rumors surfaced of a scheduled meeting on Oct. 11 between Rosenstein & members of Congress, and Rosenstein just cancelled it.
He's STILL in charge of the Mueller investigation.
He's STILL refusing to hand over the McCabe memos.
He's STILL holding up the declassification of the #SpyGate documents & their release to the public.
I love a good cover story.......
The guy had a face-to-face with El Grande Trumpo himself on Air Force One just 2 days ago. Inside just about the most secure SCIF in the world.
And Trump came out of AF1 and gave ol' Rod a big thumbs up!
And so we're right back to 'that dirty rat Rosenstein!' 2 days later.
At this point it's clear some members of Congress are either in on this and helping the cover story or they haven't got a clue and are out in the cold.
Note the conflicting stories about 'Rosenstein cancelled meeting with Congress on Oct 11!"
First, rumors surfaced of a scheduled meeting on Oct. 11 between Rosenstein & members of Congress, and Rosenstein just cancelled it.
Rep. Andy Biggs and Rep. Matt Gaetz say DAG Rod Rosenstein cancelled an Oct. 11 appearance before the judiciary and oversight committees. They are now calling for a subpoena. pic.twitter.com/TknVHKjXtd
— Ivan Pentchoukov \U0001f1fa\U0001f1f8 (@IvanPentchoukov) October 10, 2018
Keep dwelling on this:
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.
The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains
(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK
In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa
Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.
The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains
(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK
In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa
Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs