Cognitive Bias in Forensic Pathology Decisions was published today by Journal of Forensic Sciences
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An interesting thing about carp is that they can go into anoxic hibernation and switch to an anaerobic metabolism based on converting glycogen to ethanol.
The waste ethanol is diffused out the gills
https://t.co/V3D1umHf04
Carp can switch over to an anaerobic metabolism and quietly exhale booze until the situation gets better.
They basically evolved the same metabolic pathway as yeast, independently.
In theory, if you spent a few thousand years breeding carp for it, you could use them to make booze.
They'd be enormous, almost entirely glycogen deposits with a fish added as an afterthought.
The really interesting thing about anaerobic carp, is that they can go 4-5 months without oxygen by relying on liver glycogen.
You, a human, have only about 100 grams of glycogen in your liver, about 400 more grams in your skeletal muscles. Call it 500 grams total.
In humans, glycogen is also burned for energy. This is where the marathon runner's bonk comes from: you only have about 2,000 calories worth, and running a marathon burns those 2,000 calories.
The waste ethanol is diffused out the gills
https://t.co/V3D1umHf04
Carp can switch over to an anaerobic metabolism and quietly exhale booze until the situation gets better.
They basically evolved the same metabolic pathway as yeast, independently.
In theory, if you spent a few thousand years breeding carp for it, you could use them to make booze.
They'd be enormous, almost entirely glycogen deposits with a fish added as an afterthought.
The really interesting thing about anaerobic carp, is that they can go 4-5 months without oxygen by relying on liver glycogen.
You, a human, have only about 100 grams of glycogen in your liver, about 400 more grams in your skeletal muscles. Call it 500 grams total.
In humans, glycogen is also burned for energy. This is where the marathon runner's bonk comes from: you only have about 2,000 calories worth, and running a marathon burns those 2,000 calories.
It's another stunning Malagasy #dartfrog/#poisonfrog for today's #FrogOfTheDay, #42 Mantella cowani Boulenger, 1882! A highly threatened, actively conserved and managed frog from the highlands of central #Madagascar
#MadagascarFrogs
📸D.Edmonds/CalPhotos
This thread will cover only a tiny fraction of the work on Mantella cowanii because, being so charismatic and threatened, it has received quite a bit of attention.
#MadagascarFrogs
We start at the very beginning: the first specimens, two females, were collected by Reverend Deans Cowan in East Betsileo, Madagascar, and sent to London, where George Albert Boulenger described the species in 1882.
#MadagascarFrogs
Boulenger placed the species in his new genus, Mantella, along with ebenaui, betsileo, and madagascariensis. He recognised that the other Malagasy poison frogs were distinct from the Dendrobates of the Americas, although he did keep them in the Dendrobatidae.
#MadagascarFrogs
As more specimens were collected, it became clear that the species was highly variable. In 1978, Jean Guibé wrote with interest about this variability, describing a new subspecies, M. cowani nigricans—today a full species. #MadagascarFrogs
https://t.co/dwaHMbrYbj
#MadagascarFrogs
📸D.Edmonds/CalPhotos
This thread will cover only a tiny fraction of the work on Mantella cowanii because, being so charismatic and threatened, it has received quite a bit of attention.
#MadagascarFrogs
We start at the very beginning: the first specimens, two females, were collected by Reverend Deans Cowan in East Betsileo, Madagascar, and sent to London, where George Albert Boulenger described the species in 1882.
#MadagascarFrogs
Boulenger placed the species in his new genus, Mantella, along with ebenaui, betsileo, and madagascariensis. He recognised that the other Malagasy poison frogs were distinct from the Dendrobates of the Americas, although he did keep them in the Dendrobatidae.
#MadagascarFrogs
As more specimens were collected, it became clear that the species was highly variable. In 1978, Jean Guibé wrote with interest about this variability, describing a new subspecies, M. cowani nigricans—today a full species. #MadagascarFrogs
https://t.co/dwaHMbrYbj
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
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First thread of the year because I have time during MCO. As requested, a thread on the gods and spirits of Malay folk religion. Some are indigenous, some are of Indian origin, some have Islamic
Before I begin, it might be worth explaining the Malay conception of the spirit world. At its deepest level, Malay religious belief is animist. All living beings and even certain objects are said to have a soul. Natural phenomena are either controlled by or personified as spirits
Although these beings had to be respected, not all of them were powerful enough to be considered gods. Offerings would be made to the spirits that had greater influence on human life. Spells and incantations would invoke their
Two known examples of such elemental spirits that had god-like status are Raja Angin (king of the wind) and Mambang Tali Arus (spirit of river currents). There were undoubtedly many more which have been lost to time
Contact with ancient India brought the influence of Hinduism and Buddhism to SEA. What we now call Hinduism similarly developed in India out of native animism and the more formal Vedic tradition. This can be seen in the multitude of sacred animals and location-specific Hindu gods
i wonder if you can make a thread bout witchcraft in malaysia.. or list of our own local gods/deites..
— r a y a \U0001f319 (@lcvelylilith) February 20, 2020
Before I begin, it might be worth explaining the Malay conception of the spirit world. At its deepest level, Malay religious belief is animist. All living beings and even certain objects are said to have a soul. Natural phenomena are either controlled by or personified as spirits
Although these beings had to be respected, not all of them were powerful enough to be considered gods. Offerings would be made to the spirits that had greater influence on human life. Spells and incantations would invoke their
Animist ceremonies of a religious or magical nature were normally held for the purpose of divination or making a request. This would either be done at a keramat or at a shrine similar to the Thai spirit houses or Chinese roadside shrines pic.twitter.com/I1hliyi0x3
— \u2745\u1710\u170b\u1713\u170e (@uglyluhan) June 16, 2019
Two known examples of such elemental spirits that had god-like status are Raja Angin (king of the wind) and Mambang Tali Arus (spirit of river currents). There were undoubtedly many more which have been lost to time
Contact with ancient India brought the influence of Hinduism and Buddhism to SEA. What we now call Hinduism similarly developed in India out of native animism and the more formal Vedic tradition. This can be seen in the multitude of sacred animals and location-specific Hindu gods
