@NCPV @Micro_MDPI Can you comment on Professor Gallaher's statement concerning the possibility of SARS-COV-2 recombining with

@Pirbright_Inst @NCPV @Micro_MDPI Gallaher's "Prophecy" Is it Likely?
MERS + SARS-COV-2 Combo Event
MERS Situation Update - January 2020
https://t.co/K2AB0fTpJT
SA Cases:
https://t.co/ix083ozN0v
UAE Cases:
https://t.co/eCBVg3oSPo
@Pirbright_Inst @NCPV @Micro_MDPI 1. "Specificity of S is largely responsible for widespread & rapid communicability of SARS-CoV-2, with relatively low virulence in most infected; in contrast, spread of MERS is more easily contained, but its virulence is much higher, at 35%."
@Pirbright_Inst @NCPV @Micro_MDPI 2. A virus with high communicability of SARS-CoV-2 & the virulence of MERS would be apocalyptic in its impact!
There is a breakpoint sequence in MERS S (25215CAGAT) that is similarly very close in relative position to 25047CAGAT in SARS-CoV-2.....
@Pirbright_Inst @NCPV @Micro_MDPI 3. There is a 4th breakpoint sequence, between the receptor binding domain of MERS & the S1/S2 junction, that is precisely conserved in sequence & position with the corresponding location of SARS-CoV-2:
23577CAGAC in MERS
23300CAGAC in SARS-CoV-2
@Pirbright_Inst @NCPV @Micro_MDPI 4. This breakpoint, is identical in both viruses at the same relative position in the S1 protein sequence, where it defines an identical dipeptide, QT
(see underlined section in the PDF)
https://t.co/Glh98zcbWP
@Pirbright_Inst @NCPV @Micro_MDPI 5. There is potential, based on closely apposed or identical breakpoint sequences, that the bulk of the S1A and S1B domains of the S1 attachemnt subunit could be exchanged between MERS and SARS-CoV-2 in any mixed infection.
@Pirbright_Inst @NCPV @Micro_MDPI 6. While there is no indication that this has occurred in the wild, during the SARS-CoV-2 pandemic we face the unique situation of SARS-CoV-2 being present simultaneously in a substantial number of human beings worldwide.
@Pirbright_Inst @NCPV @Micro_MDPI 7. Simultaneous outbreaks of MERS, within or without areas where it has been found prevalent, could produce mixed infections in humans that we know have resulted in frequent recombination among Covs in the wild or in captive populations of animals.
@Pirbright_Inst @NCPV @Micro_MDPI 8. To the viruses, there is no known theoretical difference!
"Public health authorities should especially guard against simultaneous spread of more than one coronavirus in the human population at the same time & location"
https://t.co/O2CHJQVCXP

More from Billy Bostickson 🏴👁&👁 🆓

1/ Directed Evolution and Serial passage of RNA Viruses
A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories

2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
https://t.co/BfR0B2Hwsf


3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
https://t.co/G3mIptI54H


4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
Automation

5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
https://t.co/wdKyXghRYe
Advances in the directed evolution of proteins
https://t.co/1xXTtw1i3i
In vivo continuous directed evolution
https://t.co/xKRVSjVvo4
Emergent Bio-Kramer-BARDA-HHS-Kadlec-Fauci
U.S. Bet Big on Covid Vaccine Manufacturer Even as Problems Mounted https://t.co/o2lbFoXF5U
“Viral cross-contamination confirmed in the control cells for JANSSEN GMP Lot #8.”


an examination by The @nytimes of manufacturing practices at the Baltimore facility found serious problems, including a corporate culture that often ignored or deflected missteps and a government sponsor, BARDA, that acted more as a partner than a policeman."


https://t.co/3TszTYVhry


https://t.co/ng1oV1xrKI


https://t.co/TNQMo8LZ83
"The coronavirus in Italy since September 2019"
Unexpected detection of SARS-CoV-2 antibodies in the prepandemic period in Italy
First Published November 11, 2020
https://t.co/nhlccks5AS
Smashed Paywall thanks to @Sci_Hub
https://t.co/wWPFJMjjFJ

Maybe a coincidence but an agreement was recently signed by the authors' institute and guess who?
https://t.co/vh7qX3hyPD


The Lead Author specifically of the paper:
"Unexpected detection of SARS-CoV-2 antibodies in the prepandemic period in Italy"
is Giovanni Apolone of the "Fondazione IRCCS Istituto Nazionale Tumori" Milan, Italy


Some funding is involved....
Anyone good at Italian feel free to comment?


"You findee sars cov 2 in old sample back September time, we give you big fat red envelopeee, okey dokey?"

More has emerged regarding the Chinese connection
"Oncology research, Italy and China close to the collaboration protocol"
.https://t.co/Mt5hO9Dggc

More from Life

1/ Here’s a list of conversational frameworks I’ve picked up that have been helpful.

Please add your own.

2/ The Magic Question: "What would need to be true for you


3/ On evaluating where someone’s head is at regarding a topic they are being wishy-washy about or delaying.

“Gun to the head—what would you decide now?”

“Fast forward 6 months after your sabbatical--how would you decide: what criteria is most important to you?”

4/ Other Q’s re: decisions:

“Putting aside a list of pros/cons, what’s the *one* reason you’re doing this?” “Why is that the most important reason?”

“What’s end-game here?”

“What does success look like in a world where you pick that path?”

5/ When listening, after empathizing, and wanting to help them make their own decisions without imposing your world view:

“What would the best version of yourself do”?

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