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Saved by @BillyBostickson

@NBA @StephenKissler @yhgrad B.1.1.7 reveals clearly that SARS-CoV-2 is reverting to its original pre-outbreak condition, i.e. adapted to transgenic hACE2 mice (either Baric's BALB/c ones or others used at WIV labs during chimeric bat coronavirus experiments aimed at developing a pan betacoronavirus vaccine)

 @EricTopol @NBA @StephenKissler @yhgrad 1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice https://t.co/TNE4tuLkqN
@EricTopol @NBA @StephenKissler @yhgrad 2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2 https://t.co/B3eR764c1Z
@EricTopol @NBA @StephenKissler @yhgrad B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.
https://t.co/9Z4oJmkcKj
@EricTopol @NBA @StephenKissler @yhgrad We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the ... Thus, this mouse-adapted strain and associated challenge model should be ... (B) SARS-CoV-2 genomic RNA loads in mouse lung homogenates at P0 to P6.
https://t.co/I90OOCJg7o
@EricTopol @NBA @StephenKissler @yhgrad Mice!
A mink-associated variant carrying Δ(69-70)-Y453F-F486L-N501T-M1229I mutations was also able to utilize mouse Ace2. In addition, all variants carrying an N501Y mutation, a shared feature of UK, South Africa, & Brazil VOC strains, efficiently use mouse Ace2 orthologs.
@EricTopol @NBA @StephenKissler @yhgrad Topolino!
"Moreover, the K417N-E484K-N501Y mutations found in the South Africa variant 501Y.V2 even enable the virus to utilize rat Ace2 more efficiently than using human ACE2. These data suggest that rats and mice may (have) be(en) able to harbor and spread these variants"?
@EricTopol @NBA @StephenKissler @yhgrad Above taken from:
Circulating SARS-CoV-2 variants B.1.1.7, 501Y.V2, and P.1 have gained ability to utilize rat and mouse Ace2 and altered in vitro sensitivity to neutralizing antibodies and ACE2-Ig
https://t.co/j9qEcUz4ea
Well worth a read!
@EricTopol @NBA @StephenKissler @yhgrad even the mink variants were mouse ACE2 adapted, what a surprise...now, who would have expected that?
@EricTopol @NBA @StephenKissler @yhgrad Serial passaging of virus in mouse lungs results in adaptive mutations that increase viral infectivity
MASCp6 genome contains 5 mutations compared to its parental strain IME-BJ05, resulting in four amino acid residue changes in the ORF1ab, S, and N genes, respectively (Fig. 3A)
@EricTopol @NBA @StephenKissler @yhgrad The N501Y mutation seems to provide a more favorable interaction with mouse ACE2 for docking and entry, thus leading to the increased virulence phenotype in mice.
Adaptation of SARS-CoV-2 in BALB/c mice
https://t.co/I90OOCJg7o
@EricTopol @NBA @StephenKissler @yhgrad Protein-coding mutations in SARS-CoV-2 B.1.1.7 virus variant.
A) open reading frames (dark purple arrows) and proteins resulting from cleavage of ORF1ab polypeptide into non-structural proteins (light purple). 17 protein-coding mutations are annotated (top)
@EricTopol @NBA @StephenKissler @yhgrad which includes 14 non-synonymous mutations and 3 deletions spanning 5 viral proteins. B) Expansion of the genomic region from spike to N protein for visualization purposes.
@EricTopol @NBA @StephenKissler @yhgrad "N501Y mutation in SARS-CoV-2 spike leads to morbidity in obese and aged mice"
https://t.co/FOfZg8SiQU
@EricTopol @NBA @StephenKissler @yhgrad Differential efficiencies to neutralize the novel mutants B.1.1.7 and 501Y.V2 by collected sera from convalescent COVID-19 patients and RBD nanoparticle-vaccinated rhesus macaques
https://t.co/fBHaTllKvJ
@EricTopol @NBA @StephenKissler @yhgrad Just to annoy people
Virology: the problem with ‘leaky’ vaccines
Fresh evidence supports the theory that some vaccines lead to the evolution of more virulent viral strains
https://t.co/ttyM9J2idI

More from Billy Bostickson 🏴👁&👁 🆓

Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
@snip96581187 @Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen Clearly, because as I have been saying for 8 months now, DTRA and DARPA have been using Ecohealth and UC Davis to collect novel pathogens for gain of function work back in the USA. I have documented this in many threads which I will post here just to annoy everyone.

@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
ALL , FOR LATER READ
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
!. Simple Sabotage Tips
Unhappy with Everyday Life?
Frothing at the Mouth against oppressive bureaucracy?
Sick of petty tyrants and tin pot dictators?
Tormented by intrusive modern technology?
Feel like "sticking it to the man"?


2. The following is taken from sections 11,12 of the OSS's Simple Sabotage Field Manual, a 1944 document that has been declassified. The OSS became the CIA after WWII. The full document is

3. (a) Organizations and Conferences
Insist on doing everything through "channels." Never permit short-cuts to be taken in order to, expedite decisions.

4. Make "speeches." Talk as frequently as possible and at great length. Illustrate your "points" by long anecdotes and accounts of personal experiences.
Never hesitate to make a few appropriate "patriotic" comments.

5. When possible, refer all matters to committees, for "further study & consideration"
make the committees as large as possible - never less than 5
- Bring up irrelevant issues as frequently as possible
- Haggle over precise wordings of communications, minutes, resolutions
LIFE
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
An "Aquarium" needs cleaning in my opinion, but who am I, a mere monkey, to give advice?
Astonished at what @bellingcat discovered about GRU & FSB operations
Skripal https://t.co/PzH3a7JZvy
Navalny https://t.co/eZi63UhWw1
FSB https://t.co/sViYk9RwmB
GRU https://t.co/MY5d5gw2OG


A great post by Ilya Yahin on

Every time Putin talks about “our special services,” his signature smirk appears on his face, and in his words there is poorly concealed bragging
"Of course, we knew everything in advance"
"Yes, we are looking"
"Mercader has already been sent"
“World-class professionals”

Putin clearly loves all these spy games. He is very proud of the special services and therefore endlessly increases budget funding for them.
This is his top priority.
The elderly dictator cannot understand one thing:

there are no effective special services in a state that is completely corroded by corruption. You won't have honest professionals at your fingertips if your son-in-law buys shares of a state company for $ 100, the real value of which exceeds $ 380 million.
ALL , BUSINESS
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1. "Everything starts here, everything disappears from here, & the South China Seafood Market will become clean!"
Interesting Chinese report about the South China Seafood Market & WCDC's pathogen Lab at 288 Machang Road which makes many insinuations yet has not been censored
yet


2. "I'm here for the second time to take samples!" Shi Zhengli, a researcher at the Wuhan Institute of Virology, Chinese Academy of Sciences, appeared at the South China Seafood Market at 17:00 on March 3. On the same day, she and her team took two boxes of samples.


3. Shi Zhengli collects samples from the market to study environmental survival of the coronavirus
"It's not easy to come once" ;)
"Compared with CCDC we have taken much fewer samples, & findings are the same as their results
"They found more than we found, & we found nothing"


4. March the 3rd - 5th - The WCDC "Killing Teams" Arrive


5. "The Thin Lady"
Faced with doubts, on Feb 2, Shi Zhengli declared:
"I, Shi Zhengli, bet my life, it has nothing to do with our laboratory."
Her "statement" was widely questioned.
As of March 3, doubts were still flying, & Zhengli's appearance at the Market was eye-catching.
ALL
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
4. WTF?
Yoichi Shimatsu now brazenly accuses one of Japan's top microbiologists, Yoshihiro Kawaoka, of engineering SARS-COV-2 by inserting "HIV force multipliers", similar to his earlier work at University of Wisconsin on Influenza and HIV back in 2011.
https://t.co/ZhhZWnuKow


"I learned of Kawaoka's reckless violations of science ethics from Robert Finnegan, former editor of Jakarta Post, who was tracking theft of MERS & other virus samples from NAMRU-2 by a senior local lab technician who personally smuggled the dangerous materials to Wisconsin Uni"

"a decade ago at his lab in Wisconsin with generous funding from Japanese state institutions, Kawaoka was developing an "unstoppable flu", secretly derived from an illegal exhumation of the Arctic frozen corpse of an Alaskan native who died in the 1918-19 influenza pandemic"

Exhibit B is a 2011 research paper by Y. Kawaoka et al at his animal virology lab at University of Wisconsin:
"HIV reverse-binding protein is essential for influenza A virus replication & promotes genome-trafficking in late-stage

Favourite quote:
"The lawless pharmaceuticals, insane vaccinators & rogue microbiologists must be stopped in their tracks or humanity will face its final solution.
High-ranking sponsors in government & major corporations also should be indicted & appropriately punished by judges"
ALL

More from All

Meet Shah
Meet Shah
@ms89_meet
Strong Monopolies:

IRCTC 100% Market share in Rail Network.

IEX >90% market share in power trading.

Zydus wellness >90% market share in sugar free product.

Eicher motors >85% market share in 250cc bikes category.

MCX >85% market share in commodity trading.

Coal India >80% market share in coal production in India.

ITC >75% market share in cigarettes.

Honda Siel >75% in portable power generators.

Hindustan Zinc >75% market share in primary zinc industry.

Asahi India Glass >70% market share in automotive glass.

NRB Bearings >70% market share in needle roller bearings.

Pidilite >65% market share in adhesives.

CAMS >65% market share in RTA within mutual fund industry.

Time Technoplast >65% market share in polymer based industrial packaging.

Concor >65% market share in domestic container cargo transport.

Exide >60% market share in lead batteries.

Naukri >60% market share in the Indian job market space.

Praj >60% market share in ethanol plant installing.

Indiamart Intermesh >55% market share in the online B2B Classified space.

Borosil Renewables >55% market share in Lab glass.

Vst Tillers >50% market share in
Power tillers.

Delta corp >50% in online poker games.

Vinati Organics >50% market share in IBB.
GENERICLEARNINGS , GENERAL TWEER , INTRESTING TOPIC
Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
ALL
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libertyandjusticeforall
libertyandjusticeforal...
@Mark923to25
1. #Qanon told us that Saudi Arabia is a central piece of the puzzle in taking out the Deep State...we are watching the Deep State attempt to remove Crown Prince MBS and disrupt Pres Trump's administration's ties with MBS and the "new" Saudi/US coalition. Early in his admin,


[email protected] sent Jared Kushner (JK) to SA on an undisclosed trip and told King Salman that business as usual was over, and if he wanted Pres Trump's support, Prince Alwaleed would have to be divested of $ and power; Alwaleed is one of top Deep State $ men and was tied to HRC.


3. @Potus knew that before he could begin cleansing the US and the rest of the world of the DS, he would have to cut one of the financial head's off the snake (Alwaleed). After JK met w/ King Salman, Alwaleed was arrested and divested of billions; MBS became leader of SA.


4. SA loves Pres Trump because he's a man of his word; SA could not trust Hussein or Clintons because they use SA for oil and leverage; SA has many factions, but essentially you have the new and upcoming SA that is attempting to modernize through Crown Prince MBS, and then you


5...have the old guard represented by Alwaleed who was one of the Deep State financial heads and loyal to the DS more than SA; SA knew Hussein was financing Iran to hold western world hostage, and SA was caught in middle of Deep State black mail; this is why @Potus was celebrated
ALL
Homestays & Villas
Homestays & Villas
@thehomestays
this economical riverside resort in Manali is best for vacations

retweet follow and DM for details


@anandmahindra @himisra @_AKKaushik @TandonRaveena @kunalkamra88 tirthan manali Himachal Pradesh
ALL
Tom Gara
Tom Gara
@tomgara
This discussion between a Google engineer and their conversational AI model helped cause the engineer to believe the AI is becoming sentient, kick up an internal shitstorm and get suspended from his job. And it is absolutely insane. https://t.co/hGdwXMzQpX


FWIW I thought this was a great thread re the “is AI becoming sentient” debate, TL;DR being that it’s an interesting question but it’s dramatically less important or urgent than the actually existing issues around ethical/responsible use of AI right now


Also if AI is capable of doing things like holding lengthy conversations indistinguishable from a human, or running a business or creating beautiful art etc, whether it’s “intelligent” doesn’t really seem like the most important question either way?
ALL

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Suhas S. Hardutt
Suhas S. Hardutt
@HarduttSuhas
Marvels of Medical Science in Ancient India

ACHARYA SUSHRUTA and SUSHRUTA SAMHITA

Ancient Indians were pioneers in diverse fields of study and their genius was centuries ahead of their times. Feel the glory, basking in pride of their unparalleled achievements.

(Thread)


Sushruta (circa 7th or 6th century BC) was a surgeon who in present times is known as the “father of Indian medicine” and “father of plastic surgery” for inventing and developing surgical procedures. He lived in Kashi on the banks of river Ganga.(1)

His work documented in the Sushruta Samhita (compendium) is one of the oldest texts in the world on plastic surgery and regarded as one of the trilogies of Ayurveda. The other 2 being the Charaka Samhita and Astanga Hridaya.(2)

Sushruta compiled the Sushruta Samhita as an instruction manual for physicians to treat patients.(3)

The Samhita caters to surgical techniques listing over 300 surgical procedures and 120 surgical instruments. Additionally to 1,120 diseases, injuries and their treatments with over 700 medicinal herbs and their application.(4)
RELIGION
Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
ANCIENT BHARAT WAS CALLED VISHWAGURU, AND IT REALLY DESERVED IT.

India had a well developed education system centuries before the westerners arrived and called us uncivilized. Education was always given a great importance in Indian civilisation since times immemorial.


Ancient India had the Gurukulas and Ashramas as the epicentres of the knowledge and enlightenment. Bigger Gurukulas served as the centres of higher education called universities. Besides these universities, temples also emerged as the major learning centres.

Studying the Holy Scriptures, character building, personality development, responsibilities towards self,family and society,discipline and preservation of the ancient culture and heritage were the key embodiments of education.

This kind of education system made ancient India, a centre of knowledge all over the world. Many foreign students came to India for education and India was called the 'Vishwaguru'.
Takshshila and Nalanda were the two prominent universities of ancient India.


But were these only Universities of ancient India?The answer is 'No'.Let's learn about these gems of our education system that flourished across ancient India & be proud.

NALANDA UNIVERSITY
This was the oldest university of ancient India.Situated in Nalanda distt. of Bihar...
ALL
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SPACVet
SPACVet
@SpacVet
My number 1 stock pick for 2021 is: Pine Island Acquisition Corp.

$PIPP

Let me break down why I think this will be THE SPAC of 2021. 👇

TL:DR – the management team is loaded with President Biden’s closest friends and advisors. No other SPAC has this level of access.

$PIPP company overview:

-focused on defense, government service & aerospace
-perfect focus when you consider who is on their Management Team


$PIPP Management

-here's where it gets EXCITING
-founded by John Thain (ex Merrill Lynch CEO and Uber director)
-then STACKED with veteran Government and Administration key figures


$PIPP Insane Management Connections

-the list of PIPP "DC Partners" includes:

-- President Biden's pick for Secretary of State (Tony Blinken)
-- President Biden's pick for Secretary of Defense (Gen. Lloyd Austin).


$PIPP - The Joe Biden Connection

- Tony Blinken and President Biden are close allies.
- Blinken has been an advisor to Biden for decades
- Blinken set to be appointed Sec. of State
TRADING , CATEGORY
Twitter Data
Twitter Data
@TwitterData
r u abbreving less rn?

Since the switch to 280 characters a year ago, we've seen an increase in people writing out full words and phrases.


All about the please and thank you.

With 280 characters, people are saying 'please' (+54%) and ‘thank you’ (+22%) more.

THANKS!!!!

💬💬💬

280 characters later, there are more ? and replies to Tweets. More room, more conversations!
SOCIETY
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1. Project 1742 (EcoHealth/DTRA)
Risks of bat-borne zoonotic diseases in Western Asia

Duration: 24/10/2018-23 /10/2019

Funding: $71,500
@dgaytandzhieva
https://t.co/680CdD8uug


2. Bat Virus Database
Access to the database is limited only to those scientists participating in our ‘Bats and Coronaviruses’ project
Our intention is to eventually open up this database to the larger scientific community
https://t.co/mPn7b9HM48


3. EcoHealth Alliance & DTRA Asking for Trouble
One Health research project focused on characterizing bat diversity, bat coronavirus diversity and the risk of bat-borne zoonotic disease emergence in the region.
https://t.co/u6aUeWBGEN


4. Phelps, Olival, Epstein, Karesh - EcoHealth/DTRA


5, Methods and Expected Outcomes
(Unexpected Outcome = New Coronavirus Pandemic)
ALL