@NBA @StephenKissler @yhgrad B.1.1.7 reveals clearly that SARS-CoV-2 is reverting to its original pre-outbreak condition, i.e. adapted to transgenic hACE2 mice (either Baric's BALB/c ones or others used at WIV labs during chimeric bat coronavirus experiments aimed at developing a pan betacoronavirus vaccine)

@EricTopol @NBA @StephenKissler @yhgrad 1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice https://t.co/TNE4tuLkqN
@EricTopol @NBA @StephenKissler @yhgrad 2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2 https://t.co/B3eR764c1Z
@EricTopol @NBA @StephenKissler @yhgrad B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.
@EricTopol @NBA @StephenKissler @yhgrad We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the ... Thus, this mouse-adapted strain and associated challenge model should be ... (B) SARS-CoV-2 genomic RNA loads in mouse lung homogenates at P0 to P6.
@EricTopol @NBA @StephenKissler @yhgrad Mice!
A mink-associated variant carrying Δ(69-70)-Y453F-F486L-N501T-M1229I mutations was also able to utilize mouse Ace2. In addition, all variants carrying an N501Y mutation, a shared feature of UK, South Africa, & Brazil VOC strains, efficiently use mouse Ace2 orthologs.
@EricTopol @NBA @StephenKissler @yhgrad Topolino!
"Moreover, the K417N-E484K-N501Y mutations found in the South Africa variant 501Y.V2 even enable the virus to utilize rat Ace2 more efficiently than using human ACE2. These data suggest that rats and mice may (have) be(en) able to harbor and spread these variants"?
@EricTopol @NBA @StephenKissler @yhgrad Above taken from:
Circulating SARS-CoV-2 variants B.1.1.7, 501Y.V2, and P.1 have gained ability to utilize rat and mouse Ace2 and altered in vitro sensitivity to neutralizing antibodies and ACE2-Ig
Well worth a read!
@EricTopol @NBA @StephenKissler @yhgrad even the mink variants were mouse ACE2 adapted, what a surprise...now, who would have expected that?
@EricTopol @NBA @StephenKissler @yhgrad Serial passaging of virus in mouse lungs results in adaptive mutations that increase viral infectivity
MASCp6 genome contains 5 mutations compared to its parental strain IME-BJ05, resulting in four amino acid residue changes in the ORF1ab, S, and N genes, respectively (Fig. 3A)
@EricTopol @NBA @StephenKissler @yhgrad The N501Y mutation seems to provide a more favorable interaction with mouse ACE2 for docking and entry, thus leading to the increased virulence phenotype in mice.
Adaptation of SARS-CoV-2 in BALB/c mice
@EricTopol @NBA @StephenKissler @yhgrad Protein-coding mutations in SARS-CoV-2 B.1.1.7 virus variant.
A) open reading frames (dark purple arrows) and proteins resulting from cleavage of ORF1ab polypeptide into non-structural proteins (light purple). 17 protein-coding mutations are annotated (top)
@EricTopol @NBA @StephenKissler @yhgrad which includes 14 non-synonymous mutations and 3 deletions spanning 5 viral proteins. B) Expansion of the genomic region from spike to N protein for visualization purposes.
@EricTopol @NBA @StephenKissler @yhgrad "N501Y mutation in SARS-CoV-2 spike leads to morbidity in obese and aged mice"
@EricTopol @NBA @StephenKissler @yhgrad Differential efficiencies to neutralize the novel mutants B.1.1.7 and 501Y.V2 by collected sera from convalescent COVID-19 patients and RBD nanoparticle-vaccinated rhesus macaques
@EricTopol @NBA @StephenKissler @yhgrad Just to annoy people
Virology: the problem with ‘leaky’ vaccines
Fresh evidence supports the theory that some vaccines lead to the evolution of more virulent viral strains

More from Billy Bostickson 🏴👁&👁 🆓

1. Project 1742 (EcoHealth/DTRA)
Risks of bat-borne zoonotic diseases in Western Asia

Duration: 24/10/2018-23 /10/2019

Funding: $71,500

2. Bat Virus Database
Access to the database is limited only to those scientists participating in our ‘Bats and Coronaviruses’ project
Our intention is to eventually open up this database to the larger scientific community

3. EcoHealth Alliance & DTRA Asking for Trouble
One Health research project focused on characterizing bat diversity, bat coronavirus diversity and the risk of bat-borne zoonotic disease emergence in the region.

4. Phelps, Olival, Epstein, Karesh - EcoHealth/DTRA

5, Methods and Expected Outcomes
(Unexpected Outcome = New Coronavirus Pandemic)
#DRASTIC in the News Compilation Thread (2021)
1. Earlier stories collated

2. More examples in same thread start

3. Contributed stories

4. Washington Post (February 5th 2021)
We’re still missing the origin story of this pandemic.
China is sitting on the answers.
Archived: https://t.co/FoahR3iSRw

5. Sky News - DRASTIC Petition on TV
World 'not impressed' by WHO's COVID-19 origins report
Members @gdemaneuf and @MonaRahalkar speak out
Same covid-19 mutations are appearing in different places, which may or may not be strange....
41,000 mutations documented in 880 lineages.
Most in spike protein
What explains it?





More from All

I must say it, @K_G_Andersen never ends to surprise me with his compelling arguments... If you want to figure out why Wade is wrong you can Google it 🤣😂🤣

Here he downplays the FCS of SARS2. These fragments do not really come and go all the time. I am not aware of experiments on CoV where the FCS arose by cell or animal passage. More on the FCS here:

Here he refers to RmYN02, which has no insertion at the S1/S2 cleavage site
And to Gallaher's paper, with the fanciful theory of recombination on a train to Wuhan
Recombination is rare outside its group +

As also my co-author @ydeigin says
the FCS could be not optimal because part of a vaccine attenuation strategy
Andersen admitted to be wrong with his prediction of the O-linked glycans

The FCS might be similar at the aa level to some FCoV sequences, but less at the nt level.
Andersen does not mention that the FCS binds extremely well to heparan sulphate as possible result of cell passage

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Harvard's discriminatory policies are becoming increasingly well known, across the political spectrum (see, e.g., the recent lawsuit on discrimination against East Asian applications.)

It's refreshing to hear a senior administrator admits to personally opposing policies that attempt to remedy these basic flaws. These are flaws that harm his institution's ability to do cutting-edge research and to serve the public.

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