@NBA @StephenKissler @yhgrad B.1.1.7 reveals clearly that SARS-CoV-2 is reverting to its original pre-outbreak condition, i.e. adapted to transgenic hACE2 mice (either Baric's BALB/c ones or others used at WIV labs during chimeric bat coronavirus experiments aimed at developing a pan betacoronavirus vaccine)

@EricTopol @NBA @StephenKissler @yhgrad 1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice https://t.co/TNE4tuLkqN
@EricTopol @NBA @StephenKissler @yhgrad 2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2 https://t.co/B3eR764c1Z
@EricTopol @NBA @StephenKissler @yhgrad B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.
https://t.co/9Z4oJmkcKj
@EricTopol @NBA @StephenKissler @yhgrad We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the ... Thus, this mouse-adapted strain and associated challenge model should be ... (B) SARS-CoV-2 genomic RNA loads in mouse lung homogenates at P0 to P6.
https://t.co/I90OOCJg7o
@EricTopol @NBA @StephenKissler @yhgrad Mice!
A mink-associated variant carrying Δ(69-70)-Y453F-F486L-N501T-M1229I mutations was also able to utilize mouse Ace2. In addition, all variants carrying an N501Y mutation, a shared feature of UK, South Africa, & Brazil VOC strains, efficiently use mouse Ace2 orthologs.
@EricTopol @NBA @StephenKissler @yhgrad Topolino!
"Moreover, the K417N-E484K-N501Y mutations found in the South Africa variant 501Y.V2 even enable the virus to utilize rat Ace2 more efficiently than using human ACE2. These data suggest that rats and mice may (have) be(en) able to harbor and spread these variants"?
@EricTopol @NBA @StephenKissler @yhgrad Above taken from:
Circulating SARS-CoV-2 variants B.1.1.7, 501Y.V2, and P.1 have gained ability to utilize rat and mouse Ace2 and altered in vitro sensitivity to neutralizing antibodies and ACE2-Ig
https://t.co/j9qEcUz4ea
Well worth a read!
@EricTopol @NBA @StephenKissler @yhgrad even the mink variants were mouse ACE2 adapted, what a surprise...now, who would have expected that?
@EricTopol @NBA @StephenKissler @yhgrad Serial passaging of virus in mouse lungs results in adaptive mutations that increase viral infectivity
MASCp6 genome contains 5 mutations compared to its parental strain IME-BJ05, resulting in four amino acid residue changes in the ORF1ab, S, and N genes, respectively (Fig. 3A)
@EricTopol @NBA @StephenKissler @yhgrad The N501Y mutation seems to provide a more favorable interaction with mouse ACE2 for docking and entry, thus leading to the increased virulence phenotype in mice.
Adaptation of SARS-CoV-2 in BALB/c mice
https://t.co/I90OOCJg7o
@EricTopol @NBA @StephenKissler @yhgrad Protein-coding mutations in SARS-CoV-2 B.1.1.7 virus variant.
A) open reading frames (dark purple arrows) and proteins resulting from cleavage of ORF1ab polypeptide into non-structural proteins (light purple). 17 protein-coding mutations are annotated (top)
@EricTopol @NBA @StephenKissler @yhgrad which includes 14 non-synonymous mutations and 3 deletions spanning 5 viral proteins. B) Expansion of the genomic region from spike to N protein for visualization purposes.
@EricTopol @NBA @StephenKissler @yhgrad "N501Y mutation in SARS-CoV-2 spike leads to morbidity in obese and aged mice"
https://t.co/FOfZg8SiQU
@EricTopol @NBA @StephenKissler @yhgrad Differential efficiencies to neutralize the novel mutants B.1.1.7 and 501Y.V2 by collected sera from convalescent COVID-19 patients and RBD nanoparticle-vaccinated rhesus macaques
https://t.co/fBHaTllKvJ
@EricTopol @NBA @StephenKissler @yhgrad Just to annoy people
Virology: the problem with ‘leaky’ vaccines
Fresh evidence supports the theory that some vaccines lead to the evolution of more virulent viral strains
https://t.co/ttyM9J2idI

More from Billy Bostickson 🏴👁&👁 🆓

1. Some useful, albeit not fully developed, critiques of:

“The Origins of SARS-CoV-2: A Critical Review"

https://t.co/C9TyRyqHUr

have just been published here:
https://t.co/qCmzGCzRxn
by members of DRASTIC & others
@angoffinet @BahulikarRahul @MonaRahalkar @gdemaneuf

2. A refreshing contrast to the so-called "expert reactions" found here, including from one of the authors of the review (Prof David Robertson)!

"expert reaction to a preprint reviewing the evidence on the origins of SARS-CoV-2"

https://t.co/6kG6rstweP


3. Peter Gutierrez points out in his response that 4 of the authors of “The Origins of SARS-CoV-2: A Critical Review”, also wrote “Proximal Origin of SARS-COV-2”.

His critique focuses on:

1. RaTG13

2. Enhanced adaptation to the human host

https://t.co/eW8b2iK5xe


4. As to be expected, Sydney University and its iconic virologist, Eddie Holmes, who is "not available for interview", are crowing over this biased review
https://t.co/xrjpN1NxpO


This review contains elementary errors as exposed here:
1. https://t.co/YJ50huifly
one of the authors (Stuart Neil) then claimed
"Didn’t N501Y come up in ACE2 TG mice too?
https://t.co/HscNSEKdrZ
November 3 - 9, 2019 -Wuhan Institute of Virology
International Training Course on Biosafety Laboratory Management and Technology
1. Daniel Feakes, head of UN Biological Weapons Convention implementation support agency
2. René Courcol, quality control system expert, France's BSL


List of Lecturers with short bios at
November 3 - 9, WIV Training Course:
https://t.co/Bsd9yO40JP
YUAN Zhiming Professor, Wuhan Institute of Virology, CAS (Deputy Director)
SONG Donglin, Professor, Wuhan Institute of Virology, CAS ( Deputy Director of Wuhan BSL-4 Laboratory.)

Dr. TONG Xiao Senior Engineer at WIV (Molecular Virology & (HIV) infection, mechanical & vaccine immunology) On-site Manager & Chief engineer for WIV BSL-4 Lab construction, maintenance & biosafety equipment R&D.

Zhengli Shi, Director of WIV Center for Emerging Infectious Diseases, now in charge of scientific activity in BSL3 & BSL4. Viral pathogen discovery through sequencing techniques, wildlife-borne viral pathogens, particularly bat-borne viruses since 2004.

DENG Fei, Principle Investigator in Research Group on Viruses Resources & Biotechnology in WIV, also Director of Microorganisms & Viruses Resource Center.
Graduate of ECUST, went to Great Lakes Forestry Center, Canada in 2001 & Wageningen University, NL to study baculovirus.

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हिमालय पर्वत की एक बड़ी पवित्र गुफा थी।उस गुफा के निकट ही गंगा जी बहती थी।एक बार देवर्षि नारद विचरण करते हुए वहां आ पहुंचे।वह परम पवित्र गुफा नारद जी को अत्यंत सुहावनी लगी।वहां का मनोरम प्राकृतिक दृश्य,पर्वत,नदी और वन देख उनके हृदय में श्रीहरि विष्णु की भक्ति अत्यंत बलवती हो उठी।


और देवर्षि नारद वहीं बैठकर तपस्या में लीन हो गए।इन्द्र नारद की तपस्या से घबरा गए।उन्हें हमेशा की तरह अपना सिंहासन व स्वर्ग खोने का डर सताने लगा।इसलिए इन्द्र ने नारद की तपस्या भंग करने के लिए कामदेव को उनके पास भेज दिया।वहां पहुंच कामदेव ने अपनी माया से वसंतऋतु को उत्पन्न कर दिया।


पेड़ और पौधों पर रंग बिरंगे फूल खिल गए और कोयलें कूकने लगी,पक्षी चहकने लगे।शीतल,मंद,सुगंधित और सुहावनी हवा चलने लगी।रंभा आदि अप्सराएं नाचने लगीं ।किन्तु कामदेव की किसी भी माया का नारद पे कोई प्रभाव नहीं पड़ा।तब कामदेव को डर सताने लगा कि कहीं नारद क्रोध में आकर मुझे श्राप न देदें।

जैसे ही नारद ने अपनी आंखें खोली, उसी क्षण कामदेव ने उनसे क्षमा मांगी।नारद मुनि को तनिक भी क्रोध नहीं आया और उन्होने शीघ्र ही कामदेव को क्षमा कर दिया।कामदेव प्रसन्न होकर वहां से चले गए।कामदेव के चले जाने पर देवर्षि के मन में अहंकार आ गया कि मैने कामदेव को हरा दिया।

नारद फिर कैलाश जा पहुंचे और शिवजी को अपनी विजयगाथा सुनाई।शिव समझ गए कि नारद अहंकारी हो गए हैं और अगर ये बात विष्णु जी जान गए तो नारद के लिए अच्छा नहीं होगा।ये सोचकर शिवजी ने नारद को भगवन विष्णु को ये बात बताने के लीए मना किया। परंतु नारद जी को ये बात उचित नहीं लगी।
1

From today, we will memorize the names of 27 Nakshatras in Vedic Jyotish to never forget in life.

I will write 4 names. Repeat them in SAME sequence twice in morning, noon, evening. Each day, revise new names + recall all previously learnt names.

Pls RT if you are in.

2

Today's Nakshatras are:-

1. Ashwini - अश्विनी

2. Bharani - भरणी

3. Krittika - कृत्तिका

4. Rohini - रोहिणी

Ashwini - अश्विनी is the FIRST Nakshatra.

Repeat these names TWICE now, tomorrow morning, noon and evening. Like this tweet if you have revised 8 times as told.

3

Today's Nakshatras are:-

5. Mrigashira - मृगशिरा

6. Ardra - आर्द्रा

7. Punarvasu - पुनर्वसु

8. Pushya - पुष्य

First recall previously learnt Nakshatras twice. Then recite these TWICE now, tomorrow morning, noon & evening in SAME order. Like this tweet only after doing so.

4

Today's Nakshatras are:-

9. Ashlesha - अश्लेषा

10. Magha - मघा

11. Purvaphalguni - पूर्वाफाल्गुनी

12. Uttaraphalguni - उत्तराफाल्गुनी

Purva means that comes before (P se Purva, P se pehele), and Uttara comes later.

Read next tweet too.

5

Purva, Uttara prefixes come in other Nakshatras too. Purva= pehele wala. Remember.

First recall previously learnt 8 Nakshatras twice. Then recite those in Tweet #4 TWICE now, tomorrow morning, noon & evening in SAME order. Like this tweet if you have read Tweets #4 & 5, both.