Authors Daoyu

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Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the

chimeric infectious clone grants were there. is in 2017, Rs4231. is in 2016, RsSHC014 and RsWIV16. is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016! Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get

A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from

The Taq. to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals

Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.

The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
@franciscodeasis a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system. a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.

Marburg virus is a novel virus that escaped from the lab. the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.

The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
in fact, adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures. P681 guards the cardin-weintraub motif against cleavage in

cell lines.
the FCS is perfectly stable in anything that isn't VERO E6 classic or 293T-ACE2. anything that had TMPRSS2 and grown in trypsin-free media stably maintains the FCS.
in fact, the PRRARS, as opposed to other mutated cleavage sites--even the "perfect" H5CS--confers the greatest infectivity in CaLu-3 cells.
in fact, even P681R or S686G changes were less fit in CaLu-3 compared to PRRA virus--the P681R virus show either no difference or is slightly less effective compared to the P681 virus, and the S686G virus
@JackDempsey2_2 @pathogenetics @ggronvall

@pathogenetics @ggronvall And 100% of all the alleged market animal photos were taken in 2017. there is a reason why that animal paper refused to release the per-month data of animal sales in the wet markets—the sales were completely banned in 2018 when the consumption of

@pathogenetics @ggronvall Contraband animals were prohibited in an 2017 revision fo the CCP’s own “wildlife protection law”. This targets the main reason for consumption, which is to brag as a social status. No animals or their meat were ever sold in wet markets or online after that date. The only things

@pathogenetics @ggronvall Ever sold after the date were desiccated and sun-dried parts for use in jewelry and for medicine. They are always thoroughly dried out which would inactivate all enveloped viruses incl. all coronaviruses within them if they were present.

@pathogenetics @ggronvall
The claimed market origin is undermined by the presence of consistent positive SARS-CoV-2 detection in wastewater and in patient samples outside China in at least two different countries well into November 2019, which is before the first case in the wet
@AngloScot2 @WisdomRebel
PRRA is highly purified in the CaLu-3 cell line when FBS is added to inhibit trypsin. Stu lied about that in his defense on the FCS identity. the Gallaher article contain glaring

@WisdomRebel Mistakes and is completely impossible as HKU9 + RaTG13 only lead to a frameshift inactivated S and not a furin cleaved S.
The serological test the WIV claimed to be negative in Shi’s addendum is the exact same test they claimed positive in 2012 in the phD

@WisdomRebel thesis, and Stu once again lied about it. significant evidence in the form of contaminated SRA datasets suggest that the DEFUSE grant have been funded in China, as the full-length HKU4 is not a part of the 2016-2019 grant, but is found before the start

@WisdomRebel Date of the 2020-2024 grant of June 2020. The WIV also contained several HKU3-like Coronaviruses, particularly in mice in an 2017 GEO experiment, which is consistent with the published HKU3 and batified mice experiment in the DEFUSE document (evidence from an already conducted

@WisdomRebel Experiment). Either DEFUSE or fast-tracked form of the 2020-2024 grant in 2019 would have lead to full-length Sarbecovirus clones being used, leading to SARS-CoV-2 in the lab—they used HKU4 in stead of HKU5 for the MERS spike experiment indicating that backbone sequence distance
Bias, however, is an important problem in the WHO data. Also, an important problem seen in the points being scattered around is that they seems to be pulled toward the Huanan market—it was likely that it was used as the point of origin for the coordinate

System used. At this level of imprecision, it becomes impossible ti distinguish the Huanan market, the Wuhan CDC, and the Hankou railway station—the last of which is the main transport hub in Wuhan and the only transport hub reachable from the WIV within

1 transfer on the metro system. Cases “unlinked” were mainly clustered north of the Hankou station—which was one of the major exits through the hankou station and one of the transfer stations next to the Hankou railway station. Even connor reed, which is on the east of the

Yangtze river, supposedly have onset in November 2019 (whistleblower case #2), was connected to the WIV directly—it was on the same metro line, line 8, as the WIV. As he is an education worker, he need to commute to Wuchang where the universities are—exposing him to the WIV

Every time he takes line 8 to commute.
In addition to enforced ascertainment bias toward the market with a retrospective case search that specifically targeted the immediate surrounding of Huanan market, symptomology bias with lineage B created an