BillyBostickson | Buzz Chronicles

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

Zhengli Shi and Deyin Guo in 2015
Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Increase or Decrease Replication and Pathogenesis
https://t.co/YMlsqFOvZp

Deyin Guo 2016:
Molecular mechanisms of coronavirus RNA capping and methylation
https://t.co/xdEU5goLB6

Deyin Guo 2012
Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2′-O-methyltransferase activity of nsp10/nsp16 complex
https://t.co/x30jPYFDTa

Deyin Guo 2013
Structure-function Analysis of SARS Coronavirus RNA Cap Guanine-N7 Methyltransferase.
https://t.co/NG57b85hcs

Deyin Guo 2016
Identification and Characterization of a Ribose 2’-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales
https://t.co/zZ5V7erseD

White Coat Waste Proje...
@WhiteCoatWaste

💰🦇 2015-@NIH gives @EcoHealthNYC $3M+ to capture bats & manipulate coronaviruses at Wuhan Animal Lab
☣️2017-18-@StateDept warns Wuhan lab may cause pandemic
💸2019-@NIH re-authorizes tax $ for Wuhan lab
🦠2019-Pandemic breaks out near Wuhan lab
🔦2020-WCW exposes Wuhan-NIH link

https://t.co/iRsWg5rSkj

https://t.co/JP5I3EJU6Q

https://t.co/c257wFmmeR

https://t.co/QG20C2ngmn

Jess
@uacjess

WIV Chinese website deleted profiles:

Li Chaoyang

https://t.co/moxTfuMVY7

https://t.co/QzlUlKzNxQ

Wei Bin

https://t.co/WZpMM2uUiv

https://t.co/1lhfjateJM

Chen Jizheng

https://t.co/Ju08h54k0Y

https://t.co/IpYBBYmGMe

Huang Yi

https://t.co/Wjewoz5rYE

https://t.co/guSbINdhGw

Jamie Metzl
@JamieMetzl

1/ Having read the int’l cttee/Chinese gov’t #COVID19 origins report & watched the @WHO presser, it’s clear 1) the report is fatally flawed, 2) there’s currently no credible int’l investigation into pandemic origins, & 3) we desperately need one. Follow this thread for more.

2/ The core problem is clearly structural. The int’l team saw its task as finding a zoonotic source of animal transition in the wild, not seeking the actual source of the pandemic. There’s a big difference. They set out to prove one hypothesis, not fairly examine all of them.

3/ That’s why no one at @WHO fully recognized the massive CoI that 4 int’l committee members had prior working relationships with the #Wuhan Institute of Virology. Surprised the team took #ShiZhengli at her word rather than actually investigating?

4/ The int’l team bent over backwards trying to validate the zoonotic jump and frozen food theses but didn’t lift a finger to seriously consider the lab leak hypothesis, I’m guessing out of fear their Chinese gov’t hosts would stop tossing them table scraps if offended.

5/ It has always been my view that we need a full & unrestricted int’l forensic investigation into all origin hypotheses, including lab leak, with full access to all relevant records, samples, & personnel. Any process that does not explore all options cannot be credible.

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

PCR Drosten Fail Compilation
1. Ten Fatal Errors: Scientists Attack Paper That Established Global PCR Driven Lockdown
https://t.co/25p88F2xu6
2. Review report Corman-Drosten et al.
https://t.co/8rlT4MWoyI
3. Consequences of false

PCR Drosten Fail Compilation
4. Files for download
https://t.co/SqDrZjB9em
5. Addendum
https://t.co/IygCWvnAsP
6. False positives in reverse transcription PCR testing for SARS-CoV-2
https://t.co/HxpSZkzZ7Q
7. Discussion on Retraction

PCR Drosten Fail Compilation
8. 124 Tweet Thread by Bobby Rajesh Malhotra
@Bobby_Network
https://t.co/cdot5yIeuf
9. Another shorter thread by @Bobby_Network
https://t.co/kqFW9GsSeD
10. Thread by Abir Ballan

PCR Drosten Fail Compilation
11. Mini Thread by @Kevin_McKernan
https://t.co/pntydnd80L
12. Another longer thread by @Kevin_McKernan
https://t.co/5hBYIlXFnQ
13.

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

Keep dwelling on this:
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.

The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains

(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK

In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa

Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs

Coroldo1
@coroldo1

#SARSCOV2 #origin This thread is about analysis of origin search of SARS-COV-2 in various outbreaks in China. The 'origin search' is more for epi purpose, not quite similar as the 'origin research' in viral evolution. Of course, first cases and Huanan market include here.
1/n

#SARSCOV2 #origin First, let's have some taste of the mutation analysis of full genome sequencing with some covid-19 patients in Wuhan, by #IVDC
2/n

#SARSCOV2 #origin Then, the set up of task force to assess the food safety risk of potential transmission via cold chain.
3/n

#SARSCOV2 #origin
Initial results of the first batch of samples from Wuhan on 2nd January 2020. After Visionmedicals & BGI got their preliminary results ID as SARSr-CoV. 4/n

#SARSCOV2
4 samples received around GMT+8 12:30, pan-beta-COV(RdRp) qPCR performed, Ct value around 34. Common respiratory pathogens screening via qPCR in parallel, started viral inoculation in three cell lines, Vero E6, Huh-7, human respiratory epithelial cells.

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

Worth taking a break to watch this interview
Liu Xin's exclusive interview with China's 'Batwoman' https://t.co/Tsno7CRVys via @YouTube
1. Shi Claims ther never have been any lab leaks at P4 or the 2 P3s
2. Shi claims her lab records are transparent & available for inspection

3. Shi says all experimental work has been published
4. Shi claims her database is open for inspection!

Screenshots (1)

Screenshots (2)

Screenshots (3)

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

Compilation Thread on ORF8 ORF8 or Fate?
1. ZLS (2018):
"Considering the variability of orf8 in bats, civets and humans, investigating the function of orf8 is a priority, particularly the contribution of these different variants to viral pathogenesis."

1/2
Zheng Li Shi wrote this in 2018:
"Considering the variability of orf8 in bats, civets and humans, investigating the function of orf8 is a priority, particularly the contribution of these different variants to viral pathogenesis." https://t.co/oNHpIs1aFP
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) April 19, 2020

2. ORF8 or Fate?
"In addition to receptor binding, proteolytic cleavage of S & potentially other mutations that affect virion & trimer stability may be important for virus transmissibility in different hosts, & these factors need to be studied

3. ORF8 or Fate?
Overwhelmingly these viruses had mutations or
29 deletions in ORF8 - associated with reduced replicative fitness of the virus.. may be associated with host adaptation
via @CarltheChippy
382-nt deletion during early evolution of

4. ORF8 or Fate?
@franciscodeasis reviewed the list of read filenames according to dates and labels:
- Sat 2017-06-03 (7896)
- Sat 2017-06-17 to Tue 2017-06-20 (7896 and ORF8)
- Thu 2018-09-27 to Sun 2018-09-30 (RaTG13)
- Mon 2018-10-08 to Sun 2018-10-14 (no label and TSS)

5. ORF8 or Fate?
What is ORF8? This is Orf8!
https://t.co/21ugtiinhL

This is Orf8 pic.twitter.com/Vnkml5zyHu
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) July 16, 2020

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 STRANGE TO SEE THIS IN Wuhan Hu-1
Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1
Clostridium_difficile
Bat coronavirus BM48-31/BGR/2008
Human coronavirus 229E

@franciscodeasis @spectropan @ResearchGate @TheSeeker268 A Bulgarian Bat Virus mentioned in Lam et al:
Identification of 2019-nCoV related coronaviruses in Malayan pangolins in southern China
https://t.co/XRV8YfQ62F

@franciscodeasis @spectropan @ResearchGate @TheSeeker268 Bat coronavirus BM48-31/BGR/2008

Drosten

https://t.co/TB7hfDmsD0
and
https://t.co/JiVyAkL3H5

@franciscodeasis @spectropan @ResearchGate @TheSeeker268 Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences.
https://t.co/apTzRc4RnK

@franciscodeasis @spectropan @ResearchGate @TheSeeker268 Complete Genome Sequence of a SARS - Related Coronavirus from Kenyan Bats
Tao and Tong
https://t.co/pz17H1Abvm

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

1. I hate Tax Returns but these EcoDeath Alliance ones may be interesting to peruse:
2001: https://t.co/lws5YcYOwH
2002:
https://t.co/UOhCT2WrZR
2003:
https://t.co/GPaxtSHQWI
2005:
https://t.co/RPEK23bVzQ
2006:
https://t.co/6WYeJL2a8e
2007:

2. EcoDeath Alliance Tax Returns
2008:
https://t.co/27Pq9nx57i
2009:
https://t.co/KIcsoZP5tV
2010:
https://t.co/rJ1dq89hz9
2011:
https://t.co/txsItdtEwS
2012:

3. EcoDeath Alliance Tax Returns 2015 - 2020
2015:
https://t.co/BnMr9StUAR
2016 Audit:
https://t.co/jdBIPvAW3m
2016 Full Filing:
https://t.co/qBXdh4zeZ5
Form 990" for fiscal year ending June

4. Eco Death Alliance Tax Returns and Audits 2017
Audit for period ending June 2017
https://t.co/vjFt069KbY
Full Filing" for fiscal year ending June 2017
https://t.co/2a73SMm04U
Form 990" for fiscal year ending June

5. Eco Death Alliance Tax Returns and Audits 2018
Form 990 for period ending June 2018
https://t.co/WFdUnpe0dg
Audit for period ending June 2018
https://t.co/NwaKlSm8pV
"Full Filing" for fiscal year ending June

Coroldo1
@coroldo1

#CNIC #ZHAOXIANG #XINLIHECHUANG
Thread about Zhaoxiang et al, team of China national influenza center, one of the authors of first SARSCOV2 genomes in GISAID, evidence of funnelling money into one of his/theirs private company Xinlihechuang(Beijing).1/n

#CNIC #ZHAOXIANG #XINLIHECHUANG
Zhaoxiang's bio
2/n

#CNIC #ZHAOXIANG #XINLIHECHUANG
Wangdayan's bio
3/n

#CNIC #ZHAOXIANG #XINLIHECHUANG
Xinlihechuang(Beijing)'s product manual in 2013
4/n

#CNIC #ZHAOXIANG #XINLIHECHUANG
Please be noted: Zhaoxiang made the purchase plan, Zhaoxiang sat on the bidding panel committee to deside who won the bidding, and he chose his own company as winner as always. Here only presented a FEW cases in 2019
5/n

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

Same covid-19 mutations are appearing in different places, which may or may not be strange....
41,000 mutations documented in 880 lineages.
Most in spike protein
What explains it?

https://t.co/MvHzUb4peo

1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice https://t.co/TNE4tuLkqN
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021

https://t.co/PAwgC3lgsO

2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2 https://t.co/B3eR764c1Z
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 18, 2021

https://t.co/f9rnyFI0vq

1. Reversion of molecularly engineered, partially attenuated, very virulent infectious bursal disease virus during infection of commercial chickenshttps://t.co/wOg1Ekl3Xp
2. Reversion to Virulence of Attenuated Canine Distemper Virus In Vivo and In Vitrohttps://t.co/xhjOEYEWTY
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021

https://t.co/wAlyNzuI0W

3. pathogenic reversion of simian immunodeficiency virus SIVmac239Deltanef increases viral replicationhttps://t.co/jHmujgDVAC
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) February 24, 2021

Coroldo1
@coroldo1

#CHINACDC #SHANGHAICDC #IVDC #COMPANIES #SARSCoV2
This thread tends to give background historical information regarding authors of the first genomes and their own/affiliated private companies which benefit from outbreaks over ten year prior to covid-19.

#CHINACDC #CNIC Xiang Zhao, the author of first 3 SARS-COV-2 genomes of IVDC in GISAID, also many other papers. Started his own secret company in 2012, made home-brew kits in CNIC lab, then sell back to CNIC and CDCs all over the country via his company, 1/2

#CHINACDC #CNIC funneling national funding into own pockets. Together with him, members of CNIC and one later become key member of Shanghai Bio-germ https://t.co/0T0TlibxaA 2/2

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

1. Not surprising that @Metabiota knew first, is it?
Early Warning of COVID-19 Enabled 100%+ Revenue
Growth in 2020 for Events Software Company
https://t.co/cVs8XuKHG9

2.

4. Metabiota
Munich Re & In-Q-Tel Select Metabiota to Gain Deeper Insights into Epidemic Risk and Global Preparedness for Infectious Diseases
AUG. 2017 https://t.co/vk444G8Ivx
Previously mentioned here:https://t.co/XefQfYfBya
and here:https://t.co/IHHO6fOm9q
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) November 29, 2020

3.

1. CIA-In Q Tel-Metabiota-DTRA-Ecohealth-DARPA
Accelerating the Acquisition and Implementation of New Technologies for Intelligence:
The Report of the Independent Panel on the Central Intelligence Agency In-Q-Tel Venturehttps://t.co/rG9XVt23fN pic.twitter.com/UfS9TYBipc
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) November 29, 2020

4.

9. Global Viral Legacy Projects in Asia & Africa
Now shifted to Metabiota which works closely with Ecohealth under the aegis of DTRA & DARPAhttps://t.co/LXpEFEiuZb
Asia (China deleted only mentions Vietrnam)https://t.co/RRDs7uhm4S
Africa (PREDICT 1 & 2)https://t.co/fUa4DdBmGr pic.twitter.com/eUn4jigA8w
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) November 24, 2020

5.

April Fool's Joke or a Warning?
2019 is the year of alignment, says Gretchen Hayes
Metabiota, Munich Reinsurance and US Intel Spin-Offshttps://t.co/G0fMUFmEVq pic.twitter.com/DzmalzlBvt
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) September 2, 2020

Rossana Segreto
@Rossana38510044

1. The same “gang” of the Proximal Origin paper
https://t.co/Xmp20I58AQ
in action again (only Lipkin is missing, who knows why) to push once more faulty arguments in support of SARS2’s natural

2. The newly identified Cambodian, Thai and Japan sequences are presented as evidence that some odd features of the SARS2’s genome are natural because present in other natural viruses, as RaTG13 and pangolin CoV were used in The Proximal paper.

3. First the FCS. @ydeigin and I wrote a preprint where we show that the claimed partial FCS insertion in RmYN02 is highly doubtful and the same applies to the new bat CoV sequences

4. They present again only an amino acid biased alignment as in the Zhou paper
https://t.co/i9OZchD4LT
“adjusted by visual inspection” to push their false conclusion that all these CoVs have a partial FCS insertion at the S1/S2 junction and the FCS of SARS2 is therefore natural

5. SARS2 remains the only Sarbecovirus with a functioning FCS, as we describe here:
https://t.co/viqvfzv1Xa
and that can be easily inserted with the Seamless technology.

$Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193$

Billy Bostickson 🏴👁&👁 ...
@BillyBostickson

@fapsid @EricTopol @NBA @StephenKissler @yhgrad 1. Reversion of molecularly engineered, partially attenuated, very virulent infectious bursal disease virus during infection of commercial chickens
https://t.co/wOg1Ekl3Xp
2. Reversion to Virulence of Attenuated Canine Distemper Virus In Vivo and In

@EricTopol @NBA @StephenKissler @yhgrad 3. pathogenic reversion of simian immunodeficiency virus SIVmac239Deltanef increases viral

@EricTopol @NBA @StephenKissler @yhgrad 4. Genetic suppression in
reovirus: Ramig et al. 1977
papovavirus: Shortle et al. 1979
picornavirus: King et al. 1980
vaccinia virus: McFadden et al. 1980
influenza virus: Tolpin et al. 1981
herpesvirus: Hall and Almey 1982
adenovirus: Kruijer et al.

@EricTopol @NBA @StephenKissler @yhgrad 5. reversion to virulence of live attenuated vaccines
1. Evolutionary reversion of live viral vaccines: Can genetic engineering subdue it?
https://t.co/9lY6ji88Gr
2. Mechanisms Causing Reversion to Virulence in an Attenuated

@EricTopol @NBA @StephenKissler @yhgrad 6. reversion to virulence of live attenuated vaccines
3. The double-edged sword: How evolution can make or break a live-attenuated virus vaccine
https://t.co/jRJNTAR6tn
4. Selection Versus Mutation: Reducing the Risk of Vaccine

Coroldo1
@coroldo1

This thread is #Genbank records regarding #MN908947. Evidence only, any further analysis upon this record subjects to individuals' own discretion. So, let's roll.

1/n

2/n

3/n

4/n

$PC\U0001f30e\u262e$

PC🌎☮
@pc_432

Moderna CEO Stephane Bancel was previously CEO of bioMerieux in France from 07-10.

Alain Merieux, who owns bioMerieux, was instrumental in the creation of the Wuhan Institute of Virology P4 Lab.

The same people who helped create the virus, also helped to create the vaccines...

Moderna partnered with French Pasteur Institute in 2015 to develop mRNA vaccine technology.

Pasteur Institute partnered with the Wuhan P4 Laboratory in 2017 along with the Merieux Foundation to study emerging viruses...
https://t.co/yFsHwrNYaK
https://t.co/9M5lydBKhM

Nobel prize winning scientist Luc Montagnier asserts that Sars-Cov-2 is man-made and originated from the Wuhan Institute of Virology.

Montagnier did extensive work with the Pasteur Institute in France which was partnered with the Wuhan P4.

Merieux Foundation & the Chinese government have worked together since 1965, and partnered to study emerging pathogens in Africa in 2015.

Their research included "PATHOGENS CARRIED BY BATS" that provoke respiratory diseases.

🚨🚨🚨
https://t.co/gVwpT0ssqI

Coroldo1
@coroldo1

A thread ONLY aimed to present PREVIOUS information on PREVIOUS outbreaks. History sometimes repeats itself, so do persons/institutions/procedures. Any analysis based on reappearance of similar institutions/procedures/persons should take difference in time/tech into consideration

#MERS The 2012-2013 also had similarities, the first beta coronavirus outbreak after 2003 #SARS, person in charge was the same compared with #COVID19.
So, let's roll.

#MERS 20131015 presentation by Wenjie Tan of China CDC, on pathogenicity and detection. 1/12

#MERS 2/12

#MERS 3/12

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