Check out our last preprint- We use single-cell & spatial technologies to map the human uterus and inform organoids experiments

Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro

We generate single-cell and spatial reference maps of the human uterus and dissect the signalling pathways that determine cell fate of the epithelial lineages in the lumenal and glandular microenvironments.
To study cell-signalling we use Cell2location (@vitaliikl) and develop CellPhoneDBv3 that considers cellular coordinates for the inference of cell-cell communication
We mapped 3D endometrial organoid cultures and benchmark the model to our uterine atlas. To do so, we develop a novel computational pipeline to align in vitro and in vivo datasets.
Our comparison highlights common pathways regulating the differentiation of secretory and ciliated lineage in vivo and in vitro.
Informed by the in vivo dataset, we show in vitro that downregulation of WNT or NOTCH pathways increases the differentiation efficiency along the secretory and ciliated lineages, respectively
We are excited about how our mechanistic insights of the healthy endometrium may help us understand neglected uterine pathologies, such as endometriosis or endometrial cancer
Amazing collaborative work with Turco lab @CamPathology @teichlab @bayraktar_lab led by amazing scientists @LuzGarAl @krobertssci @NikolKons & Louis-F Hanfield
A big thank you @MoffettAm485 for all fruitful discussions & help. Such an amazing journey!
Big thanks also @luiza_moore @KSaebParsy & many others for your guidance, discussions and sample acquisition.

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I think about this a lot, both in IT and civil infrastructure. It looks so trivial to “fix” from the outside. In fact, it is incredibly draining to do the entirely crushing work of real policy changes internally. It’s harder than drafting a blank page of how the world should be.


I’m at a sort of career crisis point. In my job before, three people could contain the entire complexity of a nation-wide company’s IT infrastructure in their head.

Once you move above that mark, it becomes exponentially, far and away beyond anything I dreamed, more difficult.

And I look at candidates and know-everything’s who think it’s all so easy. Or, people who think we could burn it down with no losses and start over.

God I wish I lived in that world of triviality. In moments, I find myself regretting leaving that place of self-directed autonomy.

For ten years I knew I could build something and see results that same day. Now I’m adjusting to building something in my mind in one day, and it taking a year to do the due-diligence and edge cases and documentation and familiarization and roll-out.

That’s the hard work. It’s not technical. It’s not becoming a rockstar to peers.
These people look at me and just see another self-important idiot in Security who thinks they understand the system others live. Who thinks “bad” designs were made for no reason.
Who wasn’t there.

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