@gut_health
@bkoskella
@RSocPublishing
Check out this special feature of #ProcB edited by @bkoskella @gut_health and me. Thanks to the authors for their excellent article contributions - and all articles are #freetoread. Hopefully there is something of interest for all #microbiome researchers here @RSocPublishing
#ProcB Application of ecological and evolutionary theory to #microbiome community dynamics @jamesemcdonald @bkoskella https://t.co/fqOH0kVkaE. All Special Feature papers Free to Read - https://t.co/S02w5ODyHQ pic.twitter.com/jLk3eai1eO
— Royal Society Publishing (@RSocPublishing) January 1, 2021
@gut_health
@bkoskella
@RSocPublishing
@PhilipLeftwich
Matthew P. Edgington
and Tracey Chapman
@clydeandforth1
Martin Broberg
Sandra Denman
@jamesemcdonald
Inga Leena Angell
Knut Rudi
@CristinaHerren
@WillPerryMEFGL
Elle Lindsay, Christopher James Payne, Christopher Brodie and Raminta Kazlauskaite
@BrianTrevelline Jahree Sosa, Barry K. Hartup and
@KevinDKohl
Shen Jean Lim and @Symbionticism
Adam Ossowicki, Vittorio Tracanna, Marloes Petrus, @GillesvanWezel Jos Raaijmakers, Marnix Medema @PaolinaGarbeva
@cgreysongaito, Timothy Bartley, Karl Cottenie, Will Jarvis, @Newman_lab and @M_Stothart
@PaulineDScanlan
@M_Stothart
Rupert Palme
@Newman_lab
More from Science
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
What are the classics of the "Science of Science" or "Meta Science"? If you were teaching a class on the subject, what would go in the syllabus?
Here's a (very disorganized and incomplete) handful of suggestions, which I may add to. Suggestions welcome, especially if you've dug into relevant literatures.
1. The already classic "Estimating the reproducibility of
psychological science" from the Open Science Collaboration of @BrianNosek et al. https://t.co/yjGczLZ6Je
(Look at that abstract, wow!)
Many people had pointed out problems with standard statistical methods, going back decades (what are the best refs?). But this paper was a sledgehammer, making it impossible to ignore the question: what, if anything, were we actually learning from all those statistical studies?
2. Dean Keith Simonton's book "Creativity in Science: Chance, Logic, Genius, and Zeitgeist". If an essentially scientometric book could be described as a fun romp through science & creativity, this would be it
Here's a (very disorganized and incomplete) handful of suggestions, which I may add to. Suggestions welcome, especially if you've dug into relevant literatures.
1. The already classic "Estimating the reproducibility of
psychological science" from the Open Science Collaboration of @BrianNosek et al. https://t.co/yjGczLZ6Je
(Look at that abstract, wow!)
Many people had pointed out problems with standard statistical methods, going back decades (what are the best refs?). But this paper was a sledgehammer, making it impossible to ignore the question: what, if anything, were we actually learning from all those statistical studies?
2. Dean Keith Simonton's book "Creativity in Science: Chance, Logic, Genius, and Zeitgeist". If an essentially scientometric book could be described as a fun romp through science & creativity, this would be it
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**Thread on Bravery of Sikhs**
(I am forced to do this due to continuous hounding of Sikh Extremists since yesterday)
Rani Jindan Kaur, wife of Maharaja Ranjit Singh had illegitimate relations with Lal Singh (PM of Ranjit Singh). Along with Lal Singh, she attacked Jammu, burnt - https://t.co/EfjAq59AyI
Hindu villages of Jasrota, caused rebellion in Jammu, attacked Kishtwar.
Ancestors of Raja Ranjit Singh, The Sansi Tribe used to give daughters as concubines to Jahangir.
The Ludhiana Political Agency (Later NW Fronties Prov) was formed by less than 4000 British soldiers who advanced from Delhi and reached Ludhiana, receiving submissions of all sikh chiefs along the way. The submission of the troops of Raja of Lahore (Ranjit Singh) at Ambala.
Dabistan a contemporary book on Sikh History tells us that Guru Hargobind broke Naina devi Idol Same source describes Guru Hargobind serving a eunuch
YarKhan. (ref was proudly shared by a sikh on twitter)
Gobind Singh followed Bahadur Shah to Deccan to fight for him.
In Zafarnama, Guru Gobind Singh states that the reason he was in conflict with the Hill Rajas was that while they were worshiping idols, while he was an idol-breaker.
And idiot Hindus place him along Maharana, Prithviraj and Shivaji as saviours of Dharma.
(I am forced to do this due to continuous hounding of Sikh Extremists since yesterday)
Rani Jindan Kaur, wife of Maharaja Ranjit Singh had illegitimate relations with Lal Singh (PM of Ranjit Singh). Along with Lal Singh, she attacked Jammu, burnt - https://t.co/EfjAq59AyI
Tomorrow again same thing happens bcoz fudus like you are creating a narrative oh Khalistan. when farmers are asking MSP. (RSS ki tatti khane wale Kerni sena ke kutte).
— Ancient Economist (@_stock_tips) December 5, 2020
U kill sikhs in 1984 just politics. To BC low IQ fudu Saale entire history was politics.
Hindu villages of Jasrota, caused rebellion in Jammu, attacked Kishtwar.
Ancestors of Raja Ranjit Singh, The Sansi Tribe used to give daughters as concubines to Jahangir.
The Ludhiana Political Agency (Later NW Fronties Prov) was formed by less than 4000 British soldiers who advanced from Delhi and reached Ludhiana, receiving submissions of all sikh chiefs along the way. The submission of the troops of Raja of Lahore (Ranjit Singh) at Ambala.
Dabistan a contemporary book on Sikh History tells us that Guru Hargobind broke Naina devi Idol Same source describes Guru Hargobind serving a eunuch
YarKhan. (ref was proudly shared by a sikh on twitter)
Gobind Singh followed Bahadur Shah to Deccan to fight for him.
In Zafarnama, Guru Gobind Singh states that the reason he was in conflict with the Hill Rajas was that while they were worshiping idols, while he was an idol-breaker.
And idiot Hindus place him along Maharana, Prithviraj and Shivaji as saviours of Dharma.
I think a plausible explanation is that whatever Corbyn says or does, his critics will denounce - no matter how much hypocrisy it necessitates.
Corbyn opposes the exploitation of foreign sweatshop-workers - Labour MPs complain he's like Nigel
He speaks up in defence of migrants - Labour MPs whinge that he's not listening to the public's very real concerns about immigration:
He's wrong to prioritise Labour Party members over the public:
He's wrong to prioritise the public over Labour Party
One of the oddest features of the Labour tax row is how raising allowances, which the media allowed the LDs to describe as progressive (in spite of evidence to contrary) through the coalition years, is now seen by everyone as very right wing
— Tom Clark (@prospect_clark) November 2, 2018
Corbyn opposes the exploitation of foreign sweatshop-workers - Labour MPs complain he's like Nigel
He speaks up in defence of migrants - Labour MPs whinge that he's not listening to the public's very real concerns about immigration:
He's wrong to prioritise Labour Party members over the public:
He's wrong to prioritise the public over Labour Party
Krugman is, of course, right about this. BUT, note that universities can do a lot to revitalize declining and rural regions.
See this thing that @lymanstoneky wrote:
And see this thing that I wrote:
And see this book that @JamesFallows wrote:
And see this other thing that I wrote:
One thing I've been noticing about responses to today's column is that many people still don't get how strong the forces behind regional divergence are, and how hard to reverse 1/ https://t.co/Ft2aH1NcQt
— Paul Krugman (@paulkrugman) November 20, 2018
See this thing that @lymanstoneky wrote:
And see this thing that I wrote:
And see this book that @JamesFallows wrote:
And see this other thing that I wrote:
