Authors Billy Bostickson π΄π&π π
Coronavirus nsp10/nsp16 Methyltransferase Can Be Targeted by nsp10-Derived Peptide In Vitro and In Vivo To Increase or Decrease Replication and Pathogenesis
https://t.co/YMlsqFOvZp

Deyin Guo 2016:
Molecular mechanisms of coronavirus RNA capping and methylation
https://t.co/xdEU5goLB6

Deyin Guo 2012
Short peptides derived from the interaction domain of SARS coronavirus nonstructural protein nsp10 can suppress the 2β²-O-methyltransferase activity of nsp10/nsp16 complex
https://t.co/x30jPYFDTa

Deyin Guo 2013
Structure-function Analysis of SARS Coronavirus RNA Cap Guanine-N7 Methyltransferase.
https://t.co/NG57b85hcs

Deyin Guo 2016
Identification and Characterization of a Ribose 2β-O-Methyltransferase Encoded by the Ronivirus Branch of Nidovirales
https://t.co/zZ5V7erseD

A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories
2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
https://t.co/BfR0B2Hwsf

3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
https://t.co/G3mIptI54H

4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
Automation
5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
https://t.co/wdKyXghRYe
Advances in the directed evolution of proteins
https://t.co/1xXTtw1i3i
In vivo continuous directed evolution
https://t.co/xKRVSjVvo4

@DARPA @doddtra PREDICT & @EcoHealthNYC
What happened to FLIRT?
https://t.co/wgttFlgcP1
EcoHealth Alliance analyzed all airports in the US by assessing how often passengers trickle in from areas with confirmed cases of Zika virus.
2. βI believe this is something that people should know,β said Andrew Huff, vice-president of group, who oversees efforts to forecast the spread of infectious diseases. βIf you know where an infectious disease is, you can take precautions.β
Andrew Huff used to run their database

3. Andrew Huff
https://t.co/yqP8NbiN5X
Data papers:
https://t.co/mCdcAMTHNW
FLIRT (Zika)
https://t.co/MyUM3mt6c3
MANTLE https://t.co/dBsFAixRXv
MANTLE
4. GRITS and EcoHealth Alliance and DTRA
Global Disease Warning Software - May 28, 2015
EcoHealth Alliance and its Partners Create Biosurveillance Application with $2.2 million grant from the DTRA to continue the development of disease warning software
Global Rapid Identification Tool System (GRITS) software developed with ProMED, International Society for Infectious Disease, & Kitware
Biosurveillance app that enables infectious disease analysts to monitor textual data sources for infectious disease threats in near real time.
dsRNA viruses molecular biology
https://t.co/lwrQoo6ygG

2. Emerging Viruses Group
https://t.co/ND56gVGOAn

3. Structural Biology of Viral Genome Replication
https://t.co/wUqc0YIP3X

4. Molecular Biology of hepatitis Viruses & Gene Therapy
https://t.co/snBbxkhNsC

5. Insect Virus Genetic Engineering Lab
https://t.co/S68mVOA2Ob

From wildlife to humans with the PREDICT project
β an interview with Tracey
2. First of all we need to take into account the EcoHealth/PREDICT - Lipkin connection
https://t.co/kKtDTYQD9D
Read it carefully...
Ecohealth/DARPA and PREDICT UC Davis smuggled viruses back to the US for GOF work by Lipkin and Atlanta CDC BSL4 (Dr. Stuart Nichol) and USAMRIID

Yes, he was included above in the last tweet.
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) September 26, 2020
Lipkin is a key player in this sordid saga, who received viruses via Predict/Prophecy/Ecohealth/Darpa for experiments in the mailman lab (according to our "friend" Quammen in his book Spillover) found by @gdemaneuf pic.twitter.com/1fq20TSR3A
3. In the interview, Tracy Goldstein does not specifically clarify or state this fact, we need to read between the lines/
"examine the sequences of the coronavirus spike protein"
"examine" refers to US Lab work by Lipkin and others

4. They were sequencing as many coronaviruses as they could and experimenting on them at US Labs, seeing if they could become more pathogenic or cross the species barrier

5. Goldstein says:
"to further "understand & characterise" "prioritised viruses" to see if they can jump to humans.
Only one way to do that, isn't there?
Shipping back the deadliest viruses to the US via US consulates as exposed via leaks from Georgia
https://t.co/va6Be7w2HL

Considering international transport of pathogens under PREDICT & DTRA rules, SCH014 & possibly other viruses from Yunnan must have been sent via a US Consulate, either Wuhan or Chengdu as hand-carry items to Dr. Stuart Nichol at Atlanta CDC exactly as was done in Georgia in 2012! pic.twitter.com/owU8BMZnaD
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) September 4, 2020
Interesting expose of China's top bioweapons expert who oversaw fake pangolin research
Paper 1: https://t.co/TrXESKLYmJ
Paper 2:
https://t.co/9LSJTNCn3l
Pangolin
https://t.co/2FUAzWyOcv

Ruifu, obviously obsessed with PLAGUE,
https://t.co/7A9NiRlNoz
is a friend of Oliver Pybus (one of the Holmes, Andersen, Rambaut, Lipkin Gang)
Oliver Pybus helped Ruifu pump out CCP Propaganda
see next tweet
Pybus and Ruifu
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
https://t.co/fl8yh0PSI8
and
China's control measures may have prevented 700,000 COVID-19
Email for academic queries:
[email protected] (H.T.);
[email protected] (C.D.); [email protected] (O.G.P.);
[email protected] (B.T.G.)
[email protected] (R.Y.)

Pangoscam

I found the original H1N1-H5N1 "novel" viruses emails! Yes!
https://t.co/MoPgxYJIEv
Kawaoka Busted!
2. Kawaoka Busted!
More email evidence here:
More Smoking Gun Emails From Kobe University Virologist Teridah Ernala Ginting
Acts Of Espionage Against Indonesia by Akiko Makino and Others From Kobe University's "Secret"
3. NAMRU 2 Indonesia
(where Kawaoka organised theft of viruses)
(Now moved to Phnom Penh)
https://t.co/snRmicOIGN
History:
https://t.co/vEjltSDTOj
Perspectives on the DOD Global Emerging Infections Surveillance System:
GEIS at NAMRU 2, Indonesia (PDF)
https://t.co/SKm22oqBD4

4. NAMRU 2 Indonesia Controversy (2008)
Indonesia Suspends US NAMRU Lab Operations
https://t.co/kLW3zhiDVz
Supari On NAMRU-2
https://t.co/0wPbF4TpTJ
Indonesian Lawmakers Call For NAMRU-2 Probe
https://t.co/S4zZ4TaksW
NAMRU-2 Debate Turns
5. NAMRU Indonesia Controversy Part 2
U.S. insists on immunity for all Namru-2 laboratory staff
https://t.co/KelkEqJQTn
A "fact sheet" on NAMRU-2
https://t.co/Z08aLEDIJ4
US Embassy Half Truths:
https://t.co/b0nP0aVIwV
Indonesia: More attacks on
https://t.co/VUYbsKGncx
Kristian G. Andersen
Andrew Rambaut
Ian Lipkin
Edward C. Holmes
Robert F. Garry
2. Thanks to @newboxer007 for forwarding the link to the research by an Australian in Taiwan (not on
3. K.Andersen didn't mention "competing interests"
Only Garry listed Zalgen Labs, which we will look at later.
In acknowledgements, Michael Farzan, Wellcome Trust, NIH, ERC & ARC are mentioned.
Author affiliations listed as usual.
Note the 328 Citations!
https://t.co/nmOeohM89Q

4. Kristian Andersen (1)
Andersen worked with USAMRIID & Fort Detrick scientists on research, with Robert Garry, Jens Kuhn & Sina Bavari among
Our Hans Kristian Andersen working with Jens H. Kuhn, Sina Bavari, Robert F. Garry, Stuart T. Nichol,Gustavo Palacios, Sheli R. Radoshitzky from USAMRIID and Fort Detrick to tell more fairy tales? Full emails listed for queries...https://t.co/kLRoQTxiGD pic.twitter.com/uHNuGraPP2
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) August 26, 2020
5. Kristian Andersen (2)
Works at Scripps Research Institute, which WAS in serious financial trouble, haemorrhaging 20 million $ a year.
But just when the first virus cases were emerging, they received great news.
They issued a press release dated November 27, 2019:
Laulima Government Services (Fort Detrick BSL4) Contractor
(Alaka`ina Foundation) Hawaii DOD
Took over contract from Battelle
2/4
Tunnell Government Services
Jens Kuhn jumped ship from Battelle to steal
3/4 Jens Kuhn
https://t.co/rLxUmZoaQL
https://t.co/bOLwU9kTpW
https://t.co/WLi5NBTDEN
https://t.co/UF9gXup0El
https://t.co/5eVmln5sqN
wrote 6 tedious taxonomy papers with Fouchier
https://t.co/BAiXesvPxd
https://t.co/h4H4k7X4E3
Farzan & Kuhn ACE-2 &
4/4 Animal Origins of SARS Cov:
Insight from ACE2-S-Protein Interactions
https://t.co/sZp1fC4AdW
insights gained from study of SARS-CoV spike protein, which mediates viral entry & interaction with ACE2 receptor for SARS-CoV
Fang Li, Jens H. Kuhn, Michael Farzan

Kuhn's Appendix
https://t.co/QzTIyfdS0M
Global RNA Virome
https://t.co/zEdDcpoZKJ
S proteins of HCOVNL63 & SARS bind ACE 2 (with Farzan) https://t.co/K3RuTBo6R6!
Chapters 21, 22, 23 https://t.co/ckugrvRFwv
Chapter 57 in 1500 Page Book (Clinical Virology)
https://t.co/6ssn2LV0kS

We want to collate all references to DRASTIC in academic papers & media articles
Here are a few:
medium article by @emmecola
thorough report by @netpoette
@ColinDavdButler 's Paper
Please add any links to this thread. Tks!

2. More References
Papers by @MonaRahalkar and @BahulikarRahul
Papers by @Rossana38510044 and @ydeigin
Medium articles & papers by
@gdemaneuf & @Rdemaistre
Papers by @flavinkins (Daoyu Zhang)
Papers by "Anon" & "interneperson"
French News - le Monde
Can anyone remember any more?
3. More References
Papers & Blog Posts by @Harvard2H (Sirotkin & Sirotkin)
260 Questions for WHO collated by @billybostickson
If you find mentions of our individual names or "DRASTIC" in Papers or News, please forward here to this thread as links or screenshots.
Histoire du COVID-19 β chapitre 6 - Partie 2 : Pourquoi le sΓ©quenΓ§age complet du virus RaTG13 n'a pas Γ©tΓ© communiquΓ© par Shi Zheng Li avant fΓ©vrier 2020 ? https://t.co/MYEZZSAzaE

SARS-CoV-2: lab-origin hypothesis gains traction
BY ANNETTE GARTLAND ON OCTOBER 12, 2020
https://t.co/sPs1y8Herg

(central to coronavirus's ability to attach to host cells)
Beef, pork poultry, seafood are rich in heparan sulfate (glycosaminoglycans), highly charged anchors for SARSCoV2
Meat surfaces critical factors for transmission
https://t.co/yP6lH22OTF
1/5 Science behind that tweet just in case anyone thinks I speak without awareness ;)
Glycosaminoglycans from fresh water fish processing discard - Isolation, structural characterization, and osteogenic activity
https://t.co/fyjac03IuV
SALMON MOUTHS
2/5 Salmon Mouths 2
Characterization of Glycosaminoglycans in Gaping and Intact Connective Tissues of Farmed Atlantic Salmon (Salmo salar) Fillets by Mass
3/5 Why does SARS-COV-2 like meat & seafood?
Glycosaminoglycan binding motif at S1/S2 proteolytic cleavage site on spike glycoprotein may facilitate novel coronavirus (SARS-CoV-2) host cell entry
https://t.co/DpHF1WmxeG
Glycosaminoglycans and
4/5 Glycosaminoglycans
Role of in Infectious Disease
(same authors as above)
https://t.co/3q5dn2Io3Q
Glycosaminoglycans in Infectious Disease
https://t.co/Dep7aJlpLP
Murine Coronavirus with an Extended Host Range Uses Heparan Sulfate as an Entry
International Training Course on Biosafety Laboratory Management and Technology
1. Daniel Feakes, head of UN Biological Weapons Convention implementation support agency
2. RenΓ© Courcol, quality control system expert, France's BSL

List of Lecturers with short bios at
November 3 - 9, WIV Training Course: https://t.co/Bsd9yO40JP
YUAN Zhiming Professor, Wuhan Institute of Virology, CAS (Deputy Director)
SONG Donglin, Professor, Wuhan Institute of Virology, CAS ( Deputy Director of Wuhan BSL-4 Laboratory.)
Dr. TONG Xiao Senior Engineer at WIV (Molecular Virology & (HIV) infection, mechanical & vaccine immunology) On-site Manager & Chief engineer for WIV BSL-4 Lab construction, maintenance & biosafety equipment R&D.
Zhengli Shi, Director of WIV Center for Emerging Infectious Diseases, now in charge of scientific activity in BSL3 & BSL4. Viral pathogen discovery through sequencing techniques, wildlife-borne viral pathogens, particularly bat-borne viruses since 2004.
DENG Fei, Principle Investigator in Research Group on Viruses Resources & Biotechnology in WIV, also Director of Microorganisms & Viruses Resource Center.
Graduate of ECUST, went to Great Lakes Forestry Center, Canada in 2001 & Wageningen University, NL to study baculovirus.
The documents offer a glimpse into the military-academic complex of scientists who study how to prevent spillovers of potential pandemic pathogens from bats, raising questions about contagion risks, for example, of shipping of bats and rats infected with dangerous pathogens.

Does Rocky Mountain Laboratory ring a bell?
It should do, because that is exactly where Angela "It's all a Conspiracy" Rasmussen worked on DARPA programs
https://t.co/O22i48vG7K
No wonder, @MonaRahalkar
From Rocky Mountain to Mailman, Columbia and Lipkin

I don't like to blown my own whistle too much but I identified this PREEMPT transmissible bat vaccine research back in
PREEMPT novel transmissible bat vaccine https://t.co/PRyCWJiLzr pic.twitter.com/JLd8fKxOLu
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) October 29, 2020
and in more depth
1. Thread on Animal Vaccination - US Military
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) September 25, 2020
Prediction of Spillover Potential & Interventional En Masse Animal Vaccination to Prevent Emerging Pathogen Threats in Current and Future Zones of US Military Operationhttps://t.co/u3e229XP19
Thanks to @R_H_Ebright for paving the way
The filers of the patent included a bat coronavirus researcher and colleague of those who have researched the suspected SARS outbreak at the Mojiang mineshaft,
Remember Zhengli Shi liked to feed bats?
Maybe she did not inform Peter Daszak about
via @Biol4Ever https://t.co/ozjkXQ4Ilyhttps://t.co/260rH20LSr pic.twitter.com/4i8G2XHLEa
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) January 29, 2021
Someone had to feed all the bats that WIV researchers "bagged" to take back to their laboratory during sampling expeditions
5. Finally, a WIV researcher confesses to "bagging a dozen live bats" for further work at the laboratory
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) December 11, 2020
Thanks to @waittwoweeks for finding this useful piece of information to confound the lies of the guilty ones.https://t.co/s3ylN7t1tC pic.twitter.com/Xj2VsKc7ng
You can't have bloody bats flying around a lab, can you?
Hence the Bat Cage
16. Wuhan Institute of Virology's Patents
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) August 9, 2020
Including BAT CAGES
(A kind of carnivorism bat rearging cage)
Design for a tourniquet for treating wounds to the finger in biosafety labs filed by the Wuhan Institute of Virology on 15 Nov 2019https://t.co/V3Yc9Yrujt@interne41914499 pic.twitter.com/d2lqNiwTRL
Of course, a bat like a human gets hungry and like a human, a hungry bat is a an angry bat.
Find out how exactly Zhengli Shi fed her pet bats, it's all their in truly disgusting detail in a patent discovered by @interne41914499
Read it slowly &
I have been going back to Chmura's Thesis work after reading the confessions of bat abuse in Quammen's spillover to look for more clues as to what was going on over the last two decades with bat research in
2. A previously aborted
1/4 From an obscure PhD thesis by Aleksei Avery CHMURA, now chief of staff at @EcoHealthNYC & author of:
— Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193 (@BillyBostickson) May 11, 2020
2013 WIV paper: https://t.co/ISK9ltN7h6
2016: https://t.co/RZBWvTJro0
2018: https://t.co/MZWk571PZp
Thesis
Evaluating Risks of Paramyxovirus and Coronavirus Emergence in China
3. The Chmura Story is in Chapter 38 of Quammen's Spillover
https://t.co/mFomOog94F

4. Some details are also mentioned here
via @gdemaneuf
5. Amusing
1/ I like the
— Gilles Demaneuf (@gdemaneuf) November 24, 2020
"Since the United States forswore offensive bioweapons research back in 1969, presumably DARPA's disease program is now aimed at preventing, not creating strategic surprises of the epidemiological sort".