https://t.co/n7fagVLYm0
We assembled two mammalian expression vectors and one DNA cassette inserted into African Swine Fever from PRJNA607174! What happened in GuangDong at Mar-Aug 2019???!

The legitimacy of those “samples”—completely destroyed. The CoV-like sequences—cloned. No data from the pCoV group should ever be trusted in any way anymore!
Note: the DNA cassette exist in both unintegrated and integrated forms. Likely using homology-directed recombination. Whatever they were trying to express it is not just one or two proteins. There were also SV40 Ori which is yet to be properly mapped.
https://t.co/O1FYnwX6Oj
Why you need expression vectors in VERO since these cells are never used as expression hosts? Especially since there were a load of different tags on these vectors. The proteins had novel tags both N and C, IgK, His, Myc—especially His tag. This is for
NiNTA purification. There is no way that anyone would tag a protein this way and only use it to transfect VERO cells. There are no other host cells in these datasets other than Manis Javanica. Only Manis Javanica and Chlorocebus Aethiops. VERO is never used for recombinant
Protein production, and there were no pigs in the dataset. Vectors therefore intended for Manis Javanica cells. This mean that the GD pangolins were intentionalally transfected with expression vectors and inoculated with recombinant ASFV expressing most likely CoV antigens.
GFP can’t even pass through the secretion pathway—why fuse that behind an IGk secretion signal?
SV40 pA.
Did they GFP-tag some of these proteins and make a recombinant ASFV just to make up the symptoms and histopathology? Very likely!
ASFV particles are found in Double-membraned vesicles when assembled, ASFV particles with GFP tagging glows green granularly in tissue, ASFV causes pneumonia and thromboembolism—it is the perfect body double for a fake CoV in all and every “supporting picture” they made!
Inoculate with rASFV. Mix in some CoV pEASY-T1 clones. Add in pcDNA3.1+/SV40pA-S-E-M-N. You get the perfect fake sample for a fake CoV!
@DrLiMengYAN1
Why there were no 5’- and 3’- sequence for MP789? The SRA had these junctions erased.
So to not be immediately obvious. But the other vector backbone sequences were left behind.
Synthetic sequences invalidate the supposed origin of the samples. Either Reused VERO stock or clone-derived sequences created the trace CoV reads.

More from Health

🚨Important changes to lockdown/self-isolation regulations from 5pm

The Health Protection (Coronavirus, Restrictions) (All Tiers and Self-Isolation) (England) (Amendment) Regulations 2021

£800 'house party' FPN & police can now access track & trace data

https://t.co/k9XCpVsXhC


“Large gathering offence”

As trailed by Home Secretary last week there is now a fixed penalty notice of £800 (or £400 if you pay within 14 days) for participating in an gathering of over 15 people in a private residence


Fixed Penalty Notices double for each subsequent “large gathering offence” up to £6,400

Compare:
- Ordinary fixed penalty notice is £200 or £100 if paid in 14 days
- Holding or being involved in the holding of a gathering of over 30 people is £10,000


Second big change:

Since September has been a legal requirement to sell-isolate if you test positive/notified by Track & Trace of exposure to someone else who tested positive

Police can now be given access to NHS Track & Trace data if for the purpose of enforcement/prosecution


This will make it easier for police to enforce people breaking self-isolation rules. Currently there has been practically no enforcement.

Data says only a small proportion of people meant to be self-isolating are fully doing so.
Thread on how atheism leads to mental retardation (backed with medical citations🧵💉)

To start with, atheism is an unnatural self-contradicting doctrine.

Medical terminology proves that human beings are naturally pre-disposed to believe in God. Oxford scientists assert that people are "born believers".

https://t.co/kE0Fi588yn
https://t.co/OqyXcGIMJn


It should be known that atheism could never produce an intelligently-functioning society and neither ever will.

Contrastingly, Islam produced several intellectuals & polymaths, was on the forefront of scientific development, boasting 100% literacy


It is also scientifically proven that atheism led to lesser scientific curiosity and scientific frauds, which is also why atheists incline to pseudo-science.

Whereas, religion in general and Islam in particular boosted education.

https://t.co/19Onc84u3g


Atheists are also likely to affected by pervasive mental and developmental disorders like high-functioning autism.

Cognitive Scientists and renowned Neurologists found that more atheism is leads to greater autism.

https://t.co/zRjEyFoX3P

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