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Saved by @BillyBostickson

How SARS2 was stitched together
Nowadays,SARS2 like viruses can be generated in lab within weeks https://t.co/uWAI0oMrIU
If this was possible in Wuhan 2018/19 is debatable.
However, Shi Zhengli had 2 grants to investigate which manipulations enable bat viruses to go pandemic.
1/x

 This was pointed before by members of DRASTIC https://t.co/By9hljwjuY, @TheSeeker268 @ydeigin
CV: https://t.co/fXlqRIJoWO
Let's say the WIV did not have yeast artificial cromosomes yet, they would have had to rely on bacteria as described here:
https://t.co/F4C9HfVcXv
2/x
This method has now evolved using in vitro assembly and transcription, which significantly speeds up the process and reduces costs, as described here:
https://t.co/Qi180L3c9u
In either case, one would need a special kind of Type IIS endonucleases (DNA cleaving enzymes):
3/x
These relatively rare enzymes cut outside of their recognition site and can produce variable 4 nucleotide long DNA sticky ends. Different sticky ends enable an ordered assembly of the full viral genome by ensuring that always the right ends are stitched together by ligases.
4/x
Also, there should be not too many fragments, and fragments should not be longer than ~8 thousand bases.
All these parameters, that do not have any advantage in natural evolution, are miraculously also met by SARS2.
This backbone also enables super easy RBM/FCS manipulations.
5/x
Miraculously, the S1 spike, pangolin-derived RBM, and MERS-like FCS can all be conveniently replaced with simple BsaI digests and new inserts.
Such variants of RaTG13 could be produced for a few $100.
6/x
Lets take a look at RaTG13, the most likely template virus.
The restriction sites are much less irregular, the first fragment would have probably been to big (10.4kb)
Some sites were re-used (doesn't alter the protein sequence), others newly removed/introduced.
smoking gun#4?
7/7
Side note: all sticky ends derived from such a SARS2 digestion are different, which is required for proper reassembly. This in itself is not very unusual given there are 4^4 possible sticky ends.
Side note2: we see 1-2% mutations between RaTG13 and SARS2 on protein level, suggesting that the recombinant RaTG13-PangolinRBM-FCS CoV was subsequently cultured (=tested for infectivity) on human cells/in ACE2 transgenic animals. We could have lost a cleavage site there.
Side note3:
yes, this is no ultimate proof that SARS2 was build in a lab.
It's yet another BIG smoking gun inconsistant with natural evolution.
I picked a provokative title as virologists seem to be ok with condemning origin theories in @Nature with zero evidence these days.
Side note4:
wow, this has gone viral🙃.
I do not want to tell anybody how to interpret this.
Still, few personal notes:
-none of this indicates SARS2 was build as a weapon
-the aim of the project may have been understanding pandemics
-warnings were ignored & safety abysmal (BSL2)
-similarly risky research has been done in the US and Europe as well
-it has seen a boom since the pandemic
-it is done mostly unregistered, unregulated and under inappropriate safety conditions
-this mess could be much worse if the technology got into the hands of terrorists
😱
Side note5: for specialists:yes, some evidence suggests RaTG13 was manipulted/not the direct template of SARS2. RaTG13 could also be a cousin derived from different passaging experiments with the same, 98-99% RaTG13 identical ancestor (Ra4991?).
https://t.co/efJe7adVFX
1% difference on protein level is not that much.
Delta variant is ~1.5% different in S1 from first SARS2 samples, mutations can accumulate faster during lab passaging.
The reason that we do not know for sure is not the lack of evidence, it's the lack of access to the evidence!

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Khatvaanga
Khatvaanga
@khatvaanga
Sri Gurubhyo Namah.
Some tweets on Adi Shankaracharya and in particular the Guru Parampara of @sringerimath. [I had done this series earlier 5 years ago on account of Shishya Sweekaram].


Adi Shankara established the Sringeri Peetham as the 1st of 4 amnaaya peetams.

The four being
1. Sarada Peetam at Sringeri.
2. Govardhana Peetam at Puri
3. Kalika at Dwaraka
4. Jyothir at Badarikashramam.

He installed his 4 most prominent shishyas as heads of the peetams.

Sureshwaracharya was assigned to Sringeri as its head.

Sringeri is a corruption of Rshyashrunga giri.

Rshyashrunga maharshi [and his father Sri Vibandhaka Maharshi] did their tapas at this place.

It is at Sringeri that Shankara spent 12 of His precious 32 years!!

That gives you and idea how much He loved this place.

Rshyashruga maharshi had a horn on his forehead, a result of being born to a deer. His mere presence was enough to shower rains!!

Rshyashrunga was one who led Putrakamesthi Yaagam of Dasaratha Maharaj and which resulted in Sri Rama's birth!!
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Daoyu
Daoyu
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@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
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Khatvaanga
Khatvaanga
@khatvaanga
Starting my 2nd thread on Sringeri Parampara. This will cover the Jagadgurus after Sri Vidyaranya to Sri Shivabhinava Nrisimha Bharati.

It is really very tough to match the brilliance and achievements of Vidyaranya. After whom came 15 - Chandrashekara Bharati [1386-1389].

The next Acharya was Nrisimha Bharati [1389-1408]. Harihara raya found a town for this Acharya - Narasimhapuram. Acharya helped the king by guiding him in political affairs. He also initiated the kind with mahamantras for spiritual development.

Subsequent Acharyas were
15-Puroshottama Bharati [1408 - 1448]
16-Shankara Bharati[1448 - 1455]
17-Chandrasekhara Bharati II [1455 - 1464]
18- Nrisimha Bharati II [1464 - 1479]
19-Puroshottama Bharati II [1479 - 1517]

20-Ramachandra Bharati [1517 - 1560]. Sadashiva Raya was emporer of Vijayanagara during this time.
21-Nrisimha Bharati III [1560 - 1573]
22-Nrisimha Bharati IV [1573 - 1576]
23-Nrisimha Bharati V [1576 - 1600]

24-Abhinava Nrisimha Bharati [1600-1623]. When he visited Malahanikareswara temple for first time he noticed there was no Vinayaka there so he took piece of turmeric and drew outline of Ganesha on a pillar and offered puja.
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Angela Jamison
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@SethAbramson @buzz\_chronicles @buzz\\\_chronicles @buzz_chronicles save as category @pdfmakerapp grab @pdfmakerapp grab this @readwiseio save @readwiseio save thread @threader compile summarize @Threadreaderapp unroll please @ttttreads unroll @unrollthread.com @SaveToBookmarks
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Angela Jamison
Angela Jamison
@AnJamison
@TheLastFarm @buzz\_chronicles @buzz\\\_chronicles @buzz_chronicles save as category @pdfmakerapp grab @pdfmakerapp grab this @readwiseio save @readwiseio save thread @threader compile summarize @Threadreaderapp unroll please @ttttreads unroll @unrollthread.com @SaveToBookmarks
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Aurangzeb sent his maternal uncle Shaista Khan with powerful army over 1,50,000 on the request of Badibegum Sahiba, Adilshahi sultanate

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Shaista Khan entered Deccan to kill Chhatrapati Shivaji Maharaj & to destroy his empire. Shahista Khan captured a lot of province and fort of Chakan near Pune.

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Till 3 years he gave lot of troubles to the subjects of Chhatrapati Shivaji Maharaj and he personally made Lal Mahal his destination to live at , which was the property of Chhatrapati Shivaji Maharaj . Lal Mahal, where Chhatrapati Shivaji Maharaj spent his childhood.

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Mangottu Bhagavathi Temple
#Palakkad #Kerala
#keralatemples 🚩

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Mookken Chathen, situated on the Northern 👇


side of the temple, is guarding Devi here.
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Amme Narayana
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