Let Uncle Ralph educate you
Synthetic Viral Genomics:
Risks and Benefits for Science and Society

"Alternatively, “No See’m” sites can be used to insert foreign genes into viral, eukaryotic, or microbial genome or vector, simultaneously removing all evidence of the restriction sites that were used in the recombinant DNA manipulation"

By orientating the restriction sites as “No See’m”, the sites are removed during reassembly, leaving only the desired mutation in the final DNA product.

The dual properties of strand specificity & a variable end overhang that can be tailored to match any sequence allow for Esp3I sites to be engineered as “universal connectors” that can be joined with any other four nucleotide restriction site overhangs (e.g. EcoRI, PstX1, BamH1)

Seamless assembly (also called No See’m Sites (85)) cascades have been used to assemble full length cDNAs of the coronaviruses mouse hepatitis virus, transmissible gastroenteritis virus, infectious bronchitis virus and SARS-CoV (Refs: 85,86,87)

1. Eye and Eye

2. Mr.

3. Panic in Babylon - The Upsetter
International Panic
Universal Panic
Babylon

4. LORD KITCHENER - London Is the Place for

5. MAX ROMEO - One Step

1. Woke up this afternoon to see countless Liberal Bourgeois around the world getting their knickers in a twist about what USA has been promoting in other countries around the world for generations, and insulting the great tradition of Historical Anarchism while they are at it.

2. Some more of the same nonsense

3. Same in the UK a few years ago

4. The USA has been promoting this kind of so-called "Anarchy" for decades!
This has nothing to do with Anarchism of course, which is a completely rational philosophical & political movement (despite worthy yet sometimes misguided actions of certain brave acolytes over the years)

5. Why will there never be a coup d’etat in Washington? … Because there is no American embassy there!