Here is a tweetorial from our latest publication in @Annals_Oncology about longitudinal tracking of esophageal adenocarcinoma. #OesophagealCancer #EsophagealCancer https://t.co/QWvrzBXmK7 1/8

We sequenced 245 plasma samples from 97 patients with oesophageal adenocarcinoma using a 77 gene pan-cancer ctDNA panel. 2/8
Variants derived from previously characterised driver oesophageal adenocarcinoma genes had a significantly higher VAF than variants from other genes, indicating selection. 3/8
Peripheral blood cell samples were also sequenced for 78/97 patients. CHIP mutations were identified in 23% of cases, longitudinal tracking of CHIP variants suggested these variants were dynamic over time. 4/8
We found patients that were ctDNA positive post-surgery had a significantly poorer survival than ctDNA negative patients, and the elimination of CHIP variants improved the positive predictive value. 5/8
In summary, we demonstrate in a large, national, prospectively-collected dataset that ctDNA in plasma following surgery for EAC is prognostic for relapse. Inclusion of peripheral blood cell samples can reduce or eliminate false positives from CHIP. 6/8
In the future, post-operative ctDNA could be used to risk stratify patients into high- and low-risk groups for intensification or de-escalation of adjuvant chemotherapy. 7/8
Many thanks to our founders and all patients who participated within the OCCAMS consortium framework. The study was carried out by our brilliant PhD student Emma Ococks, medical oncologist @LizzySmyth1, postdoc @AFrankell, and postdoc @neus_snows among others @MRC_CU. 8/8
@threadreaderapp unroll please

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This is the $1mln question still without an answer: why were these workers cleaning bat guano from that abandoned mine?

Surprisingly we simply don't know.

China would have all interest in clarifying that point if for instance they were prospecting or selling guano. It did not.


What we know is that EcoHealth + WIV were sampling bat sites in the vicinity at the exact time of the workers being in that mine.

#DRASTIC wrote about this and about other oddities in the official story:

Maybe it's just one of these coincidences.

Then it gets interesting: about a year after the miners death, Olival & Epstein from EcoHealth Alliance co-authored a paper about the coronavirus risk infection from bat guano collection.

No mention of the

That paper oddly used some old bat samples collected by DARPA in 2006/7 at the famous Thai bat cave.

It never mentioned that the Thai monks have been doing this every Sunday for many many years without infection.

But most interestingly it never mentioned the Mojiang mine accident, even if the perfect timing and recycling of old DARPA bat samples seem to point to a likely knowledge of it.

Anyway, the idea was to ask for more money, as you correctly

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