How SARS2 was stitched together
Nowadays,SARS2 like viruses can be generated in lab within weeks https://t.co/uWAI0oMrIU
If this was possible in Wuhan 2018/19 is debatable.
However, Shi Zhengli had 2 grants to investigate which manipulations enable bat viruses to go pandemic.
1/x

This was pointed before by members of DRASTIC https://t.co/By9hljwjuY, @TheSeeker268 @ydeigin
CV: https://t.co/fXlqRIJoWO
Let's say the WIV did not have yeast artificial cromosomes yet, they would have had to rely on bacteria as described here:
https://t.co/F4C9HfVcXv
2/x

This method has now evolved using in vitro assembly and transcription, which significantly speeds up the process and reduces costs, as described here:
https://t.co/Qi180L3c9u
In either case, one would need a special kind of Type IIS endonucleases (DNA cleaving enzymes):
3/x

These relatively rare enzymes cut outside of their recognition site and can produce variable 4 nucleotide long DNA sticky ends. Different sticky ends enable an ordered assembly of the full viral genome by ensuring that always the right ends are stitched together by ligases.
4/x

Also, there should be not too many fragments, and fragments should not be longer than ~8 thousand bases.
All these parameters, that do not have any advantage in natural evolution, are miraculously also met by SARS2.
This backbone also enables super easy RBM/FCS manipulations.
5/x