Authors Ersa Flavinkins
7 days
30 days
All time
Recent
Popular
https://t.co/n7fagVLYm0
We assembled two mammalian expression vectors and one DNA cassette inserted into African Swine Fever from PRJNA607174! What happened in GuangDong at Mar-Aug 2019???!
The legitimacy of those “samples”—completely destroyed. The CoV-like sequences—cloned. No data from the pCoV group should ever be trusted in any way anymore!
Note: the DNA cassette exist in both unintegrated and integrated forms. Likely using homology-directed recombination. Whatever they were trying to express it is not just one or two proteins. There were also SV40 Ori which is yet to be properly mapped.
https://t.co/O1FYnwX6Oj
Why you need expression vectors in VERO since these cells are never used as expression hosts? Especially since there were a load of different tags on these vectors. The proteins had novel tags both N and C, IgK, His, Myc—especially His tag. This is for
NiNTA purification. There is no way that anyone would tag a protein this way and only use it to transfect VERO cells. There are no other host cells in these datasets other than Manis Javanica. Only Manis Javanica and Chlorocebus Aethiops. VERO is never used for recombinant
We assembled two mammalian expression vectors and one DNA cassette inserted into African Swine Fever from PRJNA607174! What happened in GuangDong at Mar-Aug 2019???!
The legitimacy of those “samples”—completely destroyed. The CoV-like sequences—cloned. No data from the pCoV group should ever be trusted in any way anymore!
Note: the DNA cassette exist in both unintegrated and integrated forms. Likely using homology-directed recombination. Whatever they were trying to express it is not just one or two proteins. There were also SV40 Ori which is yet to be properly mapped.
https://t.co/O1FYnwX6Oj
Why you need expression vectors in VERO since these cells are never used as expression hosts? Especially since there were a load of different tags on these vectors. The proteins had novel tags both N and C, IgK, His, Myc—especially His tag. This is for
NiNTA purification. There is no way that anyone would tag a protein this way and only use it to transfect VERO cells. There are no other host cells in these datasets other than Manis Javanica. Only Manis Javanica and Chlorocebus Aethiops. VERO is never used for recombinant
@BallouxFrancois https://t.co/DF40S9biYF
Unfortunately the observed ORF8 inactivation or the nsp6 SGF deletion are both indicative of T cell depletion, yet the deletion of HV and Y suggest functional B cell immunity—specifically the HV deletion is not observed in the patient nor the ORF8
Deactivation. The nsp6 SGF is located in an ER exposed loop that is conserved even in long passage within immunocompromised patients. The loop likely interacts with nsp3 and help antagonizing cellular autophagy. And human-like B cell immunity does not cause deletion here.
Cellular autophagy help display peptides on MHC class II molecules, and ORF8 removed MHC class I molecules. The loss of both functions suggest the host cytotoxic T cell immunity is not functional yet the S deletions are consistent with B cell immunity in an altered host
Environment. As the long covid-associated immnocompromisation was not found to cause either SGF deletions (or any deletions) in ORF1ab nor does it inactivate ORF8, Cytotoxic T cells are functional enough in these patients to require both functions to remain intact.
It can not be HIV since that https://t.co/yKJIxeSzlf depletes helper T cells instead of cytotoxic T cells https://t.co/h0aN2vnkLk ,ironically creating a patient condition that is B-cell deficient but not T-cell deficient.
Unfortunately the observed ORF8 inactivation or the nsp6 SGF deletion are both indicative of T cell depletion, yet the deletion of HV and Y suggest functional B cell immunity—specifically the HV deletion is not observed in the patient nor the ORF8
Deactivation. The nsp6 SGF is located in an ER exposed loop that is conserved even in long passage within immunocompromised patients. The loop likely interacts with nsp3 and help antagonizing cellular autophagy. And human-like B cell immunity does not cause deletion here.
Cellular autophagy help display peptides on MHC class II molecules, and ORF8 removed MHC class I molecules. The loss of both functions suggest the host cytotoxic T cell immunity is not functional yet the S deletions are consistent with B cell immunity in an altered host
Environment. As the long covid-associated immnocompromisation was not found to cause either SGF deletions (or any deletions) in ORF1ab nor does it inactivate ORF8, Cytotoxic T cells are functional enough in these patients to require both functions to remain intact.
It can not be HIV since that https://t.co/yKJIxeSzlf depletes helper T cells instead of cytotoxic T cells https://t.co/h0aN2vnkLk ,ironically creating a patient condition that is B-cell deficient but not T-cell deficient.