BC HEALTH
Saved by @Alex1Powell

https://t.co/n7fagVLYm0
We assembled two mammalian expression vectors and one DNA cassette inserted into African Swine Fever from PRJNA607174! What happened in GuangDong at Mar-Aug 2019???!

 The legitimacy of those “samples”—completely destroyed. The CoV-like sequences—cloned. No data from the pCoV group should ever be trusted in any way anymore!
Note: the DNA cassette exist in both unintegrated and integrated forms. Likely using homology-directed recombination. Whatever they were trying to express it is not just one or two proteins. There were also SV40 Ori which is yet to be properly mapped.
https://t.co/O1FYnwX6Oj
Why you need expression vectors in VERO since these cells are never used as expression hosts? Especially since there were a load of different tags on these vectors. The proteins had novel tags both N and C, IgK, His, Myc—especially His tag. This is for
NiNTA purification. There is no way that anyone would tag a protein this way and only use it to transfect VERO cells. There are no other host cells in these datasets other than Manis Javanica. Only Manis Javanica and Chlorocebus Aethiops. VERO is never used for recombinant
Protein production, and there were no pigs in the dataset. Vectors therefore intended for Manis Javanica cells. This mean that the GD pangolins were intentionalally transfected with expression vectors and inoculated with recombinant ASFV expressing most likely CoV antigens.
GFP can’t even pass through the secretion pathway—why fuse that behind an IGk secretion signal?
SV40 pA.
Did they GFP-tag some of these proteins and make a recombinant ASFV just to make up the symptoms and histopathology? Very likely!
ASFV particles are found in Double-membraned vesicles when assembled, ASFV particles with GFP tagging glows green granularly in tissue, ASFV causes pneumonia and thromboembolism—it is the perfect body double for a fake CoV in all and every “supporting picture” they made!
Inoculate with rASFV. Mix in some CoV pEASY-T1 clones. Add in pcDNA3.1+/SV40pA-S-E-M-N. You get the perfect fake sample for a fake CoV!
@DrLiMengYAN1
Why there were no 5’- and 3’- sequence for MP789? The SRA had these junctions erased.
So to not be immediately obvious. But the other vector backbone sequences were left behind.
Synthetic sequences invalidate the supposed origin of the samples. Either Reused VERO stock or clone-derived sequences created the trace CoV reads.

More from Health

Olumuyiwa Igbalajobi, Ph.D
Olumuyiwa Igbalajobi, ...
@olumuyiwaayo
Public Health Scholarships

This may help for those considering MS/PhD in Public Health

1. The Erasmus Mundus Joint Master Degree in Public Health in Disasters
https://t.co/1Z5qpstsSu

2. Afya Bora Global Health

3. Carl Duisberg Scholarships

https://t.co/HnNXdbWBxy

4. Commonwealth Scholarships for Developing Countries

https://t.co/3fWGf5b2OH

5. Fellowships in Public Health & Tropical

6. Fellowships to Promote Mental Health Journalism

https://t.co/MVV9PFsBJ1

7. 2021-22 Jeroen Ensink Memorial Fund

8. Paul S. Lietman Global Travel Grant for Residents & Fellows

https://t.co/qK76R495QT

9. Global Health Internships and Funding

https://t.co/FD9Gh2wXvO

10. Kofi Annan Global Health Leadership

11. MA in European Public Health

https://t.co/5x0Vr7b1j8

12. MSc in Public Health Scholarships - Maastricht University,
HEALTH
Douglas Ross MP
Douglas Ross MP
@Douglas4Moray
A thread on the latest Sturgeon-Salmond revelations. 👇
1/13

Evidence has emerged of a deliberate cover-up.

A crucial detail was apparently hidden from the public.

According to Sky, the official knew it would potentially end their career if it came to light.

This is important because it may undermine Nicola Sturgeon’s story. 2/13

Throughout the Alex Salmond affair, the First Minister has dodged questions at every turn.

She has tried to hide behind legalities, false narratives, straw men.

Every tactic in the book has been rolled out to stop the truth reaching the public. 3/13

To her utmost shame, the First Minister uses the women who were so badly let down by her government as a shield.

She argues they are being attacked, when nothing could be further from the truth.

We all want to get to the bottom of what happened here - for those women. 4/13

Instead of justice, those women have been caught in this uncivil war - that they never wanted to be part of.

They’ve been failed by basic, unforgivable errors from Nicola Sturgeon’s government.

And failed ever since by the SNP’s obstruction over what went on. 5/13
HEALTH
Advertisement
Anosognosiogenesis
Anosognosiogenesis
@pookleblinky
Fun fact: the standard advice for if a dog, cat, or human bites your hand, is to feed the bite. Push your hand further into their mouth, forcing the jaw open.

Nurses, EMTs, veterinarians, etc are taught to respond this

The advice for a human biting you is to jam your hand or arm deeper into their mouth, and rub the spot under their nose back and forth.

If you attempt to remove your hand, or forearm, the teeth do what they're supposed to and gouge out a chunk.

This vicious wolf attack illustrates proper technique.


So: every EMT and nurse who gets bitten by a human, is trained to jam their hand or forearm deeper into the mouth, feeding the bite so the jaw widens enough to remove the limb

Also, every redneck learns this along with the advice of "never run from an angry dog"
HEALTH
Dr. Adam H. Domby
Dr. Adam H. Domby
@AdamHDomby
Now you know I love to sh-t in Harvard. But I also like accuracy. So I decided to go look at Harvard’s catalog to see its lack of military history that this article describes (they only teach history of pets it claims) and what I found shocked me! Shocked me! A thread: 1/


First off, Harvard students literally have multiple sections of military history that they can take listed. (It appears these ones are taught at MIT, so they might have to walk down the street for these) but... 2/


Say they want to stay on campus...they can only take numerous classes on war and diplomacy...3/


They have an entire class on Yalta. That’s right. An entire class on Yalta. 4/


But wait! There is more! They can take the British Empire, The Fall of the Roman Empire for those wanting traditional topics... 5/
HEALTH
Rebecca Fitzgerald Lab
Rebecca Fitzgerald Lab
@RFitzgerald_lab
Here is a tweetorial from our latest publication in @Annals_Oncology about longitudinal tracking of esophageal adenocarcinoma. #OesophagealCancer #EsophagealCancer https://t.co/QWvrzBXmK7 1/8

We sequenced 245 plasma samples from 97 patients with oesophageal adenocarcinoma using a 77 gene pan-cancer ctDNA panel. 2/8


Variants derived from previously characterised driver oesophageal adenocarcinoma genes had a significantly higher VAF than variants from other genes, indicating selection. 3/8


Peripheral blood cell samples were also sequenced for 78/97 patients. CHIP mutations were identified in 23% of cases, longitudinal tracking of CHIP variants suggested these variants were dynamic over time. 4/8


We found patients that were ctDNA positive post-surgery had a significantly poorer survival than ctDNA negative patients, and the elimination of CHIP variants improved the positive predictive value. 5/8
HEALTH , FOR LATER READ

You May Also Like

Khatvaanga
Khatvaanga
@khatvaanga
Sri Gurubhyo Namah.
Some tweets on Adi Shankaracharya and in particular the Guru Parampara of @sringerimath. [I had done this series earlier 5 years ago on account of Shishya Sweekaram].


Adi Shankara established the Sringeri Peetham as the 1st of 4 amnaaya peetams.

The four being
1. Sarada Peetam at Sringeri.
2. Govardhana Peetam at Puri
3. Kalika at Dwaraka
4. Jyothir at Badarikashramam.

He installed his 4 most prominent shishyas as heads of the peetams.

Sureshwaracharya was assigned to Sringeri as its head.

Sringeri is a corruption of Rshyashrunga giri.

Rshyashrunga maharshi [and his father Sri Vibandhaka Maharshi] did their tapas at this place.

It is at Sringeri that Shankara spent 12 of His precious 32 years!!

That gives you and idea how much He loved this place.

Rshyashruga maharshi had a horn on his forehead, a result of being born to a deer. His mere presence was enough to shower rains!!

Rshyashrunga was one who led Putrakamesthi Yaagam of Dasaratha Maharaj and which resulted in Sri Rama's birth!!
ALL
Dr. Jane Clare Jones
Dr. Jane Clare Jones
@janeclarejones
So, on the subject of bonkers hyperbolic pretzeling over the Bell judgement, Grace 'destroy books I don't like & make inappropriate jokes about sterilising teenage girls' Lavery has some thoughts.

Tell me why my feminism is wrong Grace.

Oh

Well, if anyone thought the Bell judgment was going to make TRAs reconsider making massively overblown claims with no evidence backed up with nothing but a thick wadge of emotional blackmail.... HAHAHA, no one thought that.


A high court in the UK made a delimited judgment about teenager's ability to consent to puberty blockers. This puts all trans people everywhere in the world at risk.

Because if any human anywhere has any thoughts that deviate in any way from the rote line dictated by

the trans rights movement, this puts all trans people everywhere in mortal danger.

Let's be honest Grace. It doesn't put trans people at risk. It puts trans ideology at risk. Because trans ideology depends on the idea of innate gender identity, and the trans child is the

necessary material evidence of the ontology of gender identity.

That is, children are being medicalised to provide evidence to underwrite adults identities.

Nothing to see here.
LAW
Advertisement
ArmaniTalks \U0001f399\U0001f525
ArmaniTalks 🎙🔥...
@ArmaniTalks
Your Most PRECIOUS Currencies:

-Energy

-Time

-Attention

-Trust

-Love

-Loyalty

-Respect

Only give these currencies to high value people. Never spend these currencies on low value shitheads eg: trolls, weasels, snakes, naysayers etc.

'Wait, I thought money was the most important currency?'

Nah.

Once you adopt abundance mentality, you realize their is no shortage of money.

Making the dollars your most important currency will have you leading an empty life.

Time to flip your perspective.

👇

▪Energy

When you strip yourself to the core, you are an emotional creature.

Emotions are your internal worlds energy.

Harnessing that energy is crucial for leveraging yourself to obtain whatever you want.

You have a finite amount everyday, so spend it wisely.

▪Time

A second that is lost will never be returned.

You start valuing the hell out of this currency the more you mature.

As the years start adding up, you realize time is precious.

You must always have a scarcity mindset towards time.

Once you do so, you will not be lazy.

▪Attention

You can be here, but not present.

Attention is completely mental.

Giving someone your attention means you are clearing up mental bandwidth to make room for them.

Only give your attention to people who help you grow.

For the negative ones?

Ignore their existence
LIFE
Seth Abramson
Seth Abramson
@SethAbramson
(THREAD) Pre-election Russia collusion may take down Trump. It's equally possible the Trumps' pre- and post-election collusion with Saudi Arabia, the UAE, and Israel will do it—a course of collusion also connected to the Kremlin. I explain here. I hope you'll read on and retweet.


1/ I must stress how unbelievably complex the "Grand Bargain" theory of the Trump-Russia case is—a different thing from saying it's not substantiated. It's substantiated in *almost every single particular*—it just *also* happens to be very confusing. Not byzantine—just confusing.

2/ The basics: Saudi Arabia, Israel, and the UAE all view Iran as their chief regional enemy. Iran is propped up by Russia. Therefore the Saudis, Israelis, and Emiratis all need a US government willing to find a way to get the Kremlin to *stop* supporting Iran in the Middle East.

3/ The best way to get Russia to stop supporting Iran—or reduce support—was/is to drop all sanctions on Russia over its 2014 annexation of Crimea, as that'd be worth *trillions* to the Kremlin over the next decade. Everyone knew that Clinton wouldn't do this—and that Trump would.

4/ Per the NYT, on August 3, 2016, Donald Trump Jr. met secretly at Trump Tower with a Saudi and Emirati emissary, George Nader, as well as an Israeli intelligence expert, Joel Zamel, with *significant* ties to both Israeli intelligence *and* Russian oligarchs allied with Putin.
POLITICS
Aneesh Philomina Antony
Aneesh Philomina Anton...
@ProdigalTrader
If u are looking to enter a scrip just before the breakout
what would u look for?
How would u identify scrips just before breakout?

Here are a few pointers
Carefully understand the them and use at ur own discretion
(All comments r compiled from a question i posed)

1/23

Price is testing the same resistance repeatedly since long.
But now in recent times it's forming multiple candles near the same resistance with low volume.
Observe it and see for opposite move.
As soon as u get a positive candle, get in to it.

2/23

1. Volume buildup (vol > when resistance point created) along with, increase in OI, increase in delivery % on HTF
2. On LTF, price stalling around breakout area for at least 1-2hr.
3. No major events.

3/23

Stock reaching breakout LVL with HL-HH
Contraction of price and volume near it especially near 20 days ma.
If there is any breach of the latest HL it should be followed by a bullish candle with good volume.
Left side of the chart has a rally with a very steep slope of ma.

4/23

Number of wide range green bars with high volumes.
If stock is at its ATH then it adds more conviction bcz there wont b supply after that point

Consolidation below the resistance, volume of the candles & strong bullish candle closing at near highs just below the resistance

5/23
TRADING , ALL