Authors Billy Bostickson πŸ΄πŸ‘&πŸ‘ πŸ†“

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@EricTopol @NBA @StephenKissler @yhgrad B.1.1.7 reveals clearly that SARS-CoV-2 is reverting to its original pre-outbreak condition, i.e. adapted to transgenic hACE2 mice (either Baric's BALB/c ones or others used at WIV labs during chimeric bat coronavirus experiments aimed at developing a pan betacoronavirus vaccine)

@NBA @StephenKissler @yhgrad 1. From Day 1, SARS-COV-2 was very well adapted to humans .....and transgenic hACE2 Mice


@NBA @StephenKissler @yhgrad 2. High Probability of serial passaging in Transgenic Mice expressing hACE2 in genesis of SARS-COV-2


@NBA @StephenKissler @yhgrad B.1.1.7 has an unusually large number of genetic changes, ... found to date in mouse-adapted SARS-CoV2 and is also seen in ferret infections.
https://t.co/9Z4oJmkcKj


@NBA @StephenKissler @yhgrad We adapted a clinical isolate of SARS-CoV-2 by serial passaging in the ... Thus, this mouse-adapted strain and associated challenge model should be ... (B) SARS-CoV-2 genomic RNA loads in mouse lung homogenates at P0 to P6.
https://t.co/I90OOCJg7o
Same covid-19 mutations are appearing in different places, which may or may not be strange....
41,000 mutations documented in 880 lineages.
Most in spike protein
What explains it?


https://t.co/MvHzUb4peo


https://t.co/PAwgC3lgsO


https://t.co/f9rnyFI0vq


https://t.co/wAlyNzuI0W
"The coronavirus in Italy since September 2019"
Unexpected detection of SARS-CoV-2 antibodies in the prepandemic period in Italy
First Published November 11, 2020
https://t.co/nhlccks5AS
Smashed Paywall thanks to @Sci_Hub
https://t.co/wWPFJMjjFJ

Maybe a coincidence but an agreement was recently signed by the authors' institute and guess who?
https://t.co/vh7qX3hyPD


The Lead Author specifically of the paper:
"Unexpected detection of SARS-CoV-2 antibodies in the prepandemic period in Italy"
is Giovanni Apolone of the "Fondazione IRCCS Istituto Nazionale Tumori" Milan, Italy


Some funding is involved....
Anyone good at Italian feel free to comment?


"You findee sars cov 2 in old sample back September time, we give you big fat red envelopeee, okey dokey?"

More has emerged regarding the Chinese connection
"Oncology research, Italy and China close to the collaboration protocol"
.https://t.co/Mt5hO9Dggc
@snip96581187 @Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen Clearly, because as I have been saying for 8 months now, DTRA and DARPA have been using Ecohealth and UC Davis to collect novel pathogens for gain of function work back in the USA. I have documented this in many threads which I will post here just to annoy everyone.

@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
1/X Bellagio Rockefeller Initiative (Global Virome Project)
Another look at some key people in the GVP & their media statements since January 2020 about "natural origin" and impossibility of a "lab leak"
Full List of 18 scientists in photograph is here:
https://t.co/yHoAjwQjI8


2/X
We have already looked closely at Dennis Carroll, Peter Daszak, George Gao, W. Ian Lipkin & seen their public statements supporting "natural origin" & hysterical attacks on "lab leak" theories.
This thread focuses on:
Michael Kurilla
Jonna Mazet
Edward Rubin (Metabiota)

3/X
If we look at the leadership team of GVP, you will see some familiar faces:
https://t.co/WdT8sNnfmy
Most familiar is probably Peter Daszak, the wealthy Treasurer, also with the key role of Secretary.
Note: USAID PREDICT Project - EcoHealthAlliance


4/X
The "Chair" is Dr. Dennis Carroll
We will have a look at his media statements on the coronavirus pandemic by inputting his name in Twitter search box (very easy!)
Note: Previously was Director of USAID Emerging Pandemic Threats Program


5/X Dennis Carroll's video media statements (1):
CNN
(note Qinghai Thangka positioned in background which Daszak also shows off in his videos obtained during EcoHealth virus hunt with
PCR Drosten Fail Compilation
1. Ten Fatal Errors: Scientists Attack Paper That Established Global PCR Driven Lockdown
https://t.co/25p88F2xu6
2. Review report Corman-Drosten et al.
https://t.co/8rlT4MWoyI
3. Consequences of false

PCR Drosten Fail Compilation
4. Files for download
https://t.co/SqDrZjB9em
5. Addendum
https://t.co/IygCWvnAsP
6. False positives in reverse transcription PCR testing for SARS-CoV-2
https://t.co/HxpSZkzZ7Q
7. Discussion on Retraction

PCR Drosten Fail Compilation
8. 124 Tweet Thread by Bobby Rajesh Malhotra
@Bobby_Network
https://t.co/cdot5yIeuf
9. Another shorter thread by @Bobby_Network
https://t.co/kqFW9GsSeD
10. Thread by Abir Ballan

PCR Drosten Fail Compilation
11. Mini Thread by @Kevin_McKernan
https://t.co/pntydnd80L
12. Another longer thread by @Kevin_McKernan
https://t.co/5hBYIlXFnQ
13.
1/ Directed Evolution and Serial passage of RNA Viruses
A quick (I hope) thread on RNA viruses & some others, mainly for my own learning process, but also as evidence (as if it was needed) of what modern virology & microbiology is capable of doing to viruses in their laboratories

2/ In Vivo Continuous Directed Evolution
1 Recent advances in the field of in vivo continuous evolution
2 Advances in continuous culture of microbes enable continuous evolution
3 Viruses, prokaryotes, & eukaryotes mediate in vivo continuous evolution
https://t.co/BfR0B2Hwsf


3/ Continuous Directed Evolution of Biomolecules
By greatly accelerating laboratory evolution, PACE may provide solutions to otherwise intractable directed evolution problems and address novel questions about molecular evolution.
https://t.co/G3mIptI54H


4/ CDE for Strain & Protein Engineering
Inducible hypermutator systems accelerate strain evolution without compromising genome integrity.
Site-specific in vivo mutagenesis strategies have facilitated continuous protein engineering.
Automation

5/ Targeted mutagenesis:
A sniper-like diversity generator in microbial engineering
https://t.co/wdKyXghRYe
Advances in the directed evolution of proteins
https://t.co/1xXTtw1i3i
In vivo continuous directed evolution
https://t.co/xKRVSjVvo4
@Pirbright_Inst @NCPV @Micro_MDPI Can you comment on Professor Gallaher's statement concerning the possibility of SARS-COV-2 recombining with

@NCPV @Micro_MDPI Gallaher's "Prophecy" Is it Likely?
MERS + SARS-COV-2 Combo Event
MERS Situation Update - January 2020
https://t.co/K2AB0fTpJT
SA Cases:
https://t.co/ix083ozN0v
UAE Cases:
https://t.co/eCBVg3oSPo


@NCPV @Micro_MDPI 1. "Specificity of S is largely responsible for widespread & rapid communicability of SARS-CoV-2, with relatively low virulence in most infected; in contrast, spread of MERS is more easily contained, but its virulence is much higher, at 35%."

@NCPV @Micro_MDPI 2. A virus with high communicability of SARS-CoV-2 & the virulence of MERS would be apocalyptic in its impact!
There is a breakpoint sequence in MERS S (25215CAGAT) that is similarly very close in relative position to 25047CAGAT in SARS-CoV-2.....

@NCPV @Micro_MDPI 3. There is a 4th breakpoint sequence, between the receptor binding domain of MERS & the S1/S2 junction, that is precisely conserved in sequence & position with the corresponding location of SARS-CoV-2:
23577CAGAC in MERS
23300CAGAC in SARS-CoV-2
Tip from the Monkey
Pangolins, September 2019 and PLA are the key to this mystery
Stay Tuned!


1. Yang


2. A jacobin capuchin dangling a flagellin pangolin on a javelin while playing a mandolin and strangling a mannequin on a paladin's palanquin, said Saladin
More to come tomorrow!


3. Yigang Tong
https://t.co/CYtqYorhzH
Archived: https://t.co/ncz5ruwE2W


4. YT Interview
Some bats & pangolins carry viruses related with SARS-CoV-2, found in SE Asia and in Yunnan, & the pangolins carrying SARS-CoV-2 related viruses were smuggled from SE Asia, so there is a possibility that SARS-CoV-2 were coming from
The Georgian DTRA bioweapon leak is back up, grab shit while you can'
https://t.co/peAcofLvB7

thread with relevant documents from
https://t.co/peAcofLvB7
related to Gilead, DTRA and other topics
(including some bizarre and deadly inventions by an American engineer of death)


1. Category: Biological weapons delivery vehicles
UAV to spread infected insects in the air
US Patent No. 8,967,029 dated March 3, 2015
Unmanned aerial vehicle for the spread of infected insects in the air
https://t.co/W2Bsq1eKtC


2. Hollow ammunition for a capsule with toxic content
US Patent No. 8,794,155 dated August 5, 2014
The invention is an ammunition for hand-held firearms, with the help of which it is possible to hit the object with toxic substances.
https://t.co/SVZ2d2giA1


3. Toxic Substance Trap Cartridge
US Patent No. 9,052,175 dated June 9, 2015
The patent discloses a cartridge for a pistol or machine gun filled with chemical or biological agents.
https://t.co/YCtr0Fosl5
@Daoyu15 @franciscodeasis @spectropan @ResearchGate @TheSeeker268 STRANGE TO SEE THIS IN Wuhan Hu-1
Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1
Clostridium_difficile
Bat coronavirus BM48-31/BGR/2008
Human coronavirus 229E


@franciscodeasis @spectropan @ResearchGate @TheSeeker268 A Bulgarian Bat Virus mentioned in Lam et al:
Identification of 2019-nCoV related coronaviruses in Malayan pangolins in southern China
https://t.co/XRV8YfQ62F


@franciscodeasis @spectropan @ResearchGate @TheSeeker268 Bat coronavirus BM48-31/BGR/2008

Drosten

https://t.co/TB7hfDmsD0
and
https://t.co/JiVyAkL3H5


@franciscodeasis @spectropan @ResearchGate @TheSeeker268 Genomic characterization of severe acute respiratory syndrome-related coronavirus in European bats and classification of coronaviruses based on partial RNA-dependent RNA polymerase gene sequences.
https://t.co/apTzRc4RnK


@franciscodeasis @spectropan @ResearchGate @TheSeeker268 Complete Genome Sequence of a SARS - Related Coronavirus from Kenyan Bats
Tao and Tong
https://t.co/pz17H1Abvm
1. Not surprising that @Metabiota knew first, is it?
Early Warning of COVID-19 Enabled 100%+ Revenue
Growth in 2020 for Events Software Company
https://t.co/cVs8XuKHG9


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